• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.523-526
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• 1994

This study was carried out to examine the salting and desalting of small casings from hog and to determine the shelf-life during cold storage. The concentration of salt in the casings equilibrated with that of the added salt after 1 day for 10%, after 2 days for 20% after 7 days for 40% salting level. During desalting at 15 and $30^{\circ}C$, residual salt concentrations in the casings decreased to less than 1% after 1 hour for 10% salt, after 12 hours for 20% salt and after 24 hours for 40% salt. The total colony count of the freshly prepared casing was about log10 4.2. The initial microflora of the prepared casings was dominated by lactic acid bacteria. The higher the salting level, the greater the microbial growth was suppressed during 6 months of storage at refrigerator temperature ($4^{\circ}C$). A salt content of 20% is satisfactory if the casings are being stored for less than 1 month before being used.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.839-844
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• 2012

This experiment was conducted to evaluate the effects of single or multi-particle size limestone on the egg shell quality, egg production, egg quality and feed intake in laying hens. A total of 280 laying hens (ISA brown) were used in this 10-wk trial. Laying hens were randomly assigned to 4 treatments with 14 replications per treatment and 5 adjacent cages as a replication (hens were caged individually). The experimental treatments were: i) L, basal diet+10% large particle limestone; ii) LS1, basal diet+8% large particle limestone+2% small particle limestone; iii) LS2, basal diet+6% large particle limestone+4% small particle limestone; iv) S, basal diet+10% small particle limestone. The egg production was unaffected by dietary treatments. The egg weight in S treatment was lighter than other treatments (p<0.05). The egg specific gravity in S treatment was lower than other treatments (p<0.05). The eggshell strength and eggshell thickness in S treatment were decreased when compared with other dietary treatments (p<0.05). The laying hens in LS1 and LS2 treatment had a higher average feed intake than the other two treatments (p<0.05). Collectively，the dietary multi-particle size limestone supplementation could be as efficient as large particle size limestone.

• Journal of Animal Science and Technology
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• 제60권6호
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• pp.11.1-11.7
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• 2018

After pubertal, cohort of small antral follicles enters to gonadotrophin-sensitive development, called recruited follicles. This study was aimed to identify candidate genes in follicular cyclic recruitment via analysis of protein-protein interaction (PPI) network. Differentially expressed genes (DEGs) in ovine granulosa cells of small antral follicles between follicular and luteal phases were accumulated among gene/protein symbols of the Ensembl annotation. Following directed graphs, PTPN6 and FYN have the highest indegree and outdegree, respectively. Since, these hubs being up-regulated in ovine granulosa cells of small antral follicles during the follicular phase, it represents an accumulation of blood immune cells in follicular phase in comparison with luteal phase. By contrast, the up-regulated hubs in the luteal phase including CDK1, INSRR and TOP2A which stimulated DNA replication and proliferation of granulosa cells, they known as candidate genes of the cyclic recruitment.

• 한국임상수의학회지
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• 제24권3호
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• pp.467-472
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• 2007

Alimentary lymphoma accounts for approximately 5% of neoplasm and diffuse lesion is more common than solitary nodular form in dogs. An eleven year-old male Yorkshire terrier was examined because of nonspecific gastrointestinal symptoms such as anorexia and vomiting for 10 days. An abdominal mass was palpated, which was originated from small intestinal wall in abdominal ultrasonography. Small intestine was obstructed by hypoechoic mass and lost normal layering and measured 24.5m. After fine-needle aspiration, septic peritonitis due to intestinal rupture occurred and emergency surgery was performed. Solitary mass was found in small intestine and diagnosed as alimentary lymphoma through histopathologic examination. Conclusively, abdominal ultrasonouaphy could verify the thickened bowel, loss of wall layering and decrease of motility and percutaneous ultrasound-guided fine-needle aspiration is considered as useful diagnostic technique, especially in nodular form of alimentary lymphoma.

• 한국임상수의학회지
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• 제23권3호
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• pp.325-328
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• 2006

10년령의 암컷 Miniature Schnauzer개가 지속적인 식욕결핍, 이장열, 구토, 복통 등의 증상으로 내원하였다. 혈액검사 상에서 백혈구 수치는 만성 염증 소견을 나타내었고, 혈액 화학검사에서는 심한 담즙정체를 나타내는 중등도의 간담도계 이상 지표를 보여주었다. 복부 방사선과 초음파상에서 간종대, 담낭내 슬러지와 결석, 방광내 결석등이 관찰되었다. 이러한 진단 소견을 바탕으로 본 증례를 방광 결석증이 합병된 담낭내 담석과 슬러지에 의한 간외성 담관 폐색증으로 진단하였다. 담석과 방광결석을 담낭절제술과 방광절제술을 통해 담낭과 방광으로부터 각각 제거하였으며, 수술 후 환축의 임상증상 개선은 매우 빠르게 진행되었다.

• 대한의용생체공학회:의공학회지
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• 제25권2호
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• pp.97-102
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• 2004

소 동물 촬영에 이용될 수 있는 고해상도의 x-선 cone-beam micro computed tomography (micro-CT) 시스템을 개발하였다 Micro-CT 시스템은 120${\times}$120 mm2 의 2차원 평판 x-선 감지기, micro-focus x-선 발생장치, 주사 기구부, 병렬처리 영상 재구성 시스템으로 이루어져 있다. 개발된 시스템의 성능을 평가하기 위해 대조도와 공간해상도를 측정하였다. 대조도 실험에서는 95 mGy 에서 36 CT-번호를 구별할 수 있음을 확인하였고, 공간 해상도 실험에서는 14 lp/mm 의 성능을 확인하였다. 소 동물 촬영 결과로 실험용 쥐의 대퇴부, 심장 그리고 복부 동맥 혈관을 촬영한 경상을 제시하였다. 개발된 micro-CT 시스템은 소 동물을 이용한 생명공학 분야 연구에 널리 이용될 수 있을 것으로 기대된다.

• Journal of Chest Surgery
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• 제30권6호
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• pp.566-572
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• 1997

유전자 치료등 폐암에 대한 새로운 치료법의 개발및 그 효능의 검증에 있어 적절한 동물 모델이 없음은 큰 제 한점중의 하나이다. 특히 종양의 생물학적 특성이나 치료에의 효과등이 장기자체의 환경에 크게 영향 을 받는다는 사실은, 인체에서의 폐암의 특성을 가지며 폐에 정 위적으로 발생하는 폐암의 동물모델의 개발 을 시급하게 한다. 저자등은 Nude rat을 대상 동물로 하여, 개흉하에 종양세포 부유액을 원하는 폐말단 부위에 직접 주입함으로 폐에 정 위적으로 폐암의 발달을 유도하였으며 이를 이용하여 발생된 비소세포 폐암의 병태를 연구하였다. 종양은 실험 대상 등물에서 모두 발생하였으며 이용한 두 가지 종류의 세포주(NCI-H46O과 NCI-H1299)에서 모두 효과적으로 발생하였다. 발생된 폐종양은 시간 경과에 따라 주위 조직으로의 침윤과 종격동 전이의 양상를 보였다. 종양 숙주 동물의 평균 수명은 약 5주 정도였다. 저자등이 개발한 비소세포폐암의 동물 모델은 기관지를 통한 종양 세포 주입법에 의한 폐암 모델에 비해 국소적으로 진행된 폐암을 원하는 부위에 정확히 만들 수 있음은 물론 외과적 처치를 비롯한 국소적 치료 방법의 개발이나 ?과의 검증에 두루 이용되기에 적절하다고 사료된다.

• Toxicological Research
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• 제29권1호
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• pp.1-6
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• 2013

The process of drug discovery and development requires substantial resources and time. The drug industry has tried to reduce costs by conducting appropriate animal studies together with molecular biological and genetic analyses. Basic science research has been limited to in vitro studies of cellular processes and ex vivo tissue examination using suitable animal models of disease. However, in the past two decades new technologies have been developed that permit the imaging of live animals using radiotracer emission, X-rays, magnetic resonance signals, fluorescence, and bioluminescence. The main objective of this review is to provide an overview of small animal molecular imaging, with a focus on nuclear imaging (single photon emission computed tomography and positron emission tomography). These technologies permit visualization of toxicodynamics as well as toxicity to specific organs by directly monitoring drug accumulation and assessing physiological and/or molecular alterations. Nuclear imaging technology has great potential for improving the efficiency of the drug development process.

• 센서학회지
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• 제26권4호
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• pp.245-250
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• 2017

A novel method to implement a birdcage-type receiving coil sensor for use in a magnetic resonance imaging(MRI) system has been demonstrated employing a flexible printed circuit board (FPCB) fabrication technique. Unlike the conventional methods, the two-dimensional shape of the coil sensor is first implemented as a FPCB and then it is attached to the surface of a cylindrical supporting structure to implement the three-dimensional birdcage-type coil sensor. The proposed method is very effective to implement object-specific MRI coil sensors especially for small animal measurements in research and preclinical applications since the existing well-developed FPCB-based techniques can easily meet the requirements on accuracies and costs during coil implement process. The performances of the coil sensor verified through $^1H$ 1.5T MRI measurements for small animals and it showed excellent characteristics by providing a high spatial precision and a high signal-to-noise ratio.

• 한국임상수의학회지
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• 제36권2호
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• pp.93-97
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• 2019

Healing of 48 cases of radial fractures in small breed dogs treated with acrylic pin external fixation was evaluated retrospectively to investigate the factors affecting the healing of radial fractures. The mean age of the subjects was 15.02 months, the mean body weight was 3.48 kg, and the mean maximum length of the radius (MLR) was 90.77 mm. External skeletal fixation frames type 1a, 2a, and 3 were used; among them type 2a was used most frequently (75%). The mean time to clinical union (TCU) was 67.17 days, and the success rate was 95.8%. Age, bodyweight, MLR, and occurrence of complications increased TCU significantly. Among complications, pin loosening, swelling of the operated forelimb tissue for more than 2 weeks, and coexistence of two or more complications increased TCU significantly (p < 0.05). There was no relationship between location of the fracture and TCU. The results of this study may be useful to predict the prognosis of radial fractures in small dogs.