To evaluate the effects of slow release fertilizer and chemical dispersant on oil biodegradation, mesocosm studies were conducted on sand seashore. The rapid removal rates (85%) of aliphatic hydrocarbons and the simultaneous decreases of n-$C_{17}$/pristane (69%) and $n-C_{18}/phytane$ (61%) ratios by the addition of slow-release fertilizer (SRF) within 37 days of experiment indicated that SRF could enhance the oil degrading activity of indigenous microorganisms in sand mesocosm. Although the growth of heterotrophic bacteria and petroleumdegrading bacteria in the mesocosm treated with $Corexit 9527^{R}$ was stimulated, the biological oil removal based on the ratios of $Corexit 9527^{R}$ and $n-C_{18}/phytane$ was inhibited. Removal rates of aliphatic hydrocarbons (56%), and n-$C_{17}$/pristane (27%) and $n-C_{18}/phytane$ (17%) ratios by the addition of chemical dispersant $Corexit 9527^{R}$ were similar or lower than those values of control (50, 60, 46%), respectively. The biodegradation activity, however, when simultaneously treated with SRF and $Corexit 9527^{R}$, was not highly inhibited and even recovered after the elimination of chemical dispersant. From these results it could be concluded that the addition of SRF enhanced the oil removal rate in oligotrophic sand seashore and chemical dispersant possibly inhibit the oil biodegradation. Hence, in order to prevent the unrestrained usage of chemical dispersant in natural environments contaminated with oil, the National Contingency Plan of Oil Spill Response should be carefully revised in consideration of the application for bioremedaition techniques.
CHOI, SUNG-CHAN;KAE KYOUNG KWON;JAE HAK SOHN;SANG-JIN KIM
Journal of Microbiology and Biotechnology
/
v.12
no.3
/
pp.431-436
/
2002
Effects of fertilizer additions for oil degradation were examined in sand seashore mesocosms. Within 37 days, up to $85\%$ removal was achieved by the addition of slow-release type fertilizer (SRF) with the initial degradation rate of 423.3 mg oil $(kg sand)^-1\;day^-1$. The removal was mostly of biological origin based on the changes of $C_17$ /pristane and $C_18$/phytane ratios from 2.60 to 0.81 and from 3.55 to 1.29, respectively. The addition of oleophilic fertilizer (Inipol EAP22) was less effective and resulted in the removal of $64\%$ of the added oil ($3\%$, v/v) with a lower initial degradation rate. Petroleum-degrading bacteria had achieved a value of $1{\times}10^8$ CFU $(g sand)^-1$ at Day 3 and this peak exactly coincided with the initial degradation in the SRF-treated mesocosm. In this mesocosm, surface tension values were decreased drastically during Days 3 and 8, suggesting that microbially-produced surface-active agents actively enhanced the oil degradation rate and cell proliferation. Although the Inipol-treated mesocosm appeared to show significantly enhanced oil degradation compared to that of the untreated control mesocosm, Inipol was found to be less effective than SRF in enhancing a true oil-degrader when compared under similar experimental conditions.
Kim, Daegyun;Cho, Kwangrae;Won, Taejin;Bak, In-Tae;Yoo, Gayoung
Korean Journal of Environmental Biology
/
v.32
no.4
/
pp.327-334
/
2014
Emission reduction of $CH_4$ (methane gas) from rice paddy soil is a very important measure for climate change mitigation in agricultural sector. In this study, we investigated the changes in crop yield and $CH_4$ emissions in response to application of biochar and fertilizers. The experimental site is located in Hwasung, Kyunggido and experimental design is the split-plot method with three replicates. Treatments included rice straw (RS) and biochar (BC) amendments nested with the conventional NPK fertilizer (NPK) and slow release fertilizer (SRF). Control was also prepared with the soil with the conventional NPK fertilization with no amendment. Measurement of $CH_4$ emission was conducted during the growing season of 2014 using a dynamic chamber method. The results showed that application of rice straw increased daily $CH_4$ emission rate by 15%, while application of biochar reduced daily $CH_4$ emission rate by 38%. When we combined biochar application with slow release fertilizer, $CH_4$ emission was reduced by 45%. Further, the crop yield was also increased in all treatments compared with the control except for the treatment of rice straw application with slow release fertilizer. Overall results imply that biochar amendment to agricultural soil can be an effective strategy to decrease annual $CH_4$ emission with no reduction in crop yield.
To find out the optimum application method of slow-releasing fertilizer(SRF) and conventional fertilizer(CF) with different fertilization rate under two culture methods[l0-day old seedling machine transplanting(MT) and direct-sowing on dry paddy(DS)] in the south-western region(clay loam soil) of Korea, used were Chosun slow-releasing fertilizer(silicate latex coated fertilizer: N-P$_2$O$_{5}$-K$_2$O =18-12-13) and conventional fertilizer. Plant height and number of tillers with different two culture methods were higher at MT than DS in early growth. The ratio of dry weight in heading stage was higher at CF than SRF in MT than DS and especially, SRF 80% + CF 20% than SRF 100% or CF 100%. Leaf area index (LAI) in heading stage was higher at CF in MT but higher at SRF in DS than their counterparts. Chlorophyll content was higher at SRF than in CF expect for heading stage(HS), especially in DS. It was highest at HS in CF without its difference during maximum tillering stage(MTS) and panicle formation stage(PFS), while highest at PFS in SRF with tendency of gradual increase and decrease before and after PFS, respectively. Heading was delayed 2~3 days at SRF in two cultrue methods and 4~5 days at SRF in DS in comparison with CF in MT with delay of 2 days at DS compared with MT. Culm length was longer at CF in MT and at SRF in DS than their counterparts. Panicle number per m was more at SRF and in DS. Filled grain ratio was higher at CF and in MT. Yield was obtained 101 and 100% at 100% and 80% level of SRF in DS respectively, and 96% at 80% level of CF in MT, compared with conventional application method (516kg /l0a), and increased 2~4% at DS and 0~3% at MT in SRF. Yield was high in order of 100%(SRF) =80%(SRF) + 20%(CF) > 100%(SRF) + 20%(CF) > 80%(SRF) at MT and 80%(SRF) + 20%(CF) =100%(SRF) > 80%(SRF) =100%(SRF) + 20%(CF) at DS.
This experiment was conducted to determine the rice quality between slowlelease fertilizer(SRF) and conventional fertilizer (CF) with the amount of 100% and 80% under direct seeding on dry paddy field, respectively. In rice quality, the percentage of matured grain and immatured grain was higher in CF with machine transplanting than in SRF with direct seeding, and the less amount of fertilizer applied, the lower percentage of immatured grain was obtained. The percentage of carbohydrate showed the difference between CF and SRF, and higher at the level of 100% applicated than that of 80% application. The contents of fat and ash were higher in CF with less fertilizer, and there was no trend to different cultural methods but it was related to the amount of fertilizer in amylose content. The nitrogen and sodium content of milled rice was high in SRF under machine transplanting as well as direct seeding. Calcium and magnesium content showed high in direct seeding but there was not significantly difference between CF and SRF.
Kim, Young-Sun;Kim, Tack-Soo;Ham, Suon-Kyu;Ban, Su-Won;Lee, Chang-Eun
Asian Journal of Turfgrass Science
/
v.23
no.1
/
pp.111-122
/
2009
Compound fertilizer contained slow release nitrogen was used at golf course management because of promoting turfgrass growth and decreasing fertilizing labor. But, it was hardly published about turf grass growth by its effect and form like isobutylidene diurea(IBDU) and methylene urea(MU) in golf course management of Korea. This study was conducted to investigate the effect of compound fertilizer contained slow release nitrogen such as IBDU and MU on creeping bentgrasss and the change in nitrogen in the root zone after application of fertilizers at AM turfgrass research institute with soil analysis, chlorophyll content index, leaf area index and dry weight during 5 months from July to december in 2007 year. Fertilizer treatments were designed as following; non-fertilizer(NF), straight fertilizer(CF), treatment 1(MU), treatment 2(IBDU 1), and treatment 3(IBDU 2). The every treatments were arranged a randomized complete block design with three replications. Results obtained were summarized as follows: As relative to time after N fertilizers application, ammonium and nitrate in soil were increased, but T-N was decrease. $NO_3$-N and T-N content of slow release fertilizer(SRF), the treatment MU, IBDU 1 and IBDU 2, in soil was higher than that of CF, and the change of ratio of available N and T-N by elapsed time after fertilizing SRFs and CF was higher about 5% in root zone of SF to l5days, but in root zone of SRF from 30days about $5{\sim}10%$. Compared with NF, the turf color index was increased 6.5%, 6.7%, 5.9% and 5.5%, respectively, the chlorophyll content 33.2%, 38.4%, 35.1% and 37.1%, the dry weight 76.2%, 77.7%, 69.5 and 72.3% and shoot number 28%, 52%, 56% and 51%. The chlorophyll content index of MD, IBDU 1 and IBDU 2 was increased $2.6{\sim}5.2%$ than that of CF, shoot number $18{\sim}22%$ but turf color index and dry weight was analogous to that of CF. These results showed that the SRF application was supplied nitrogen for a long time in root zone by increasing an available nitrogen, and so bring turf growth such as chlorophyll content and shoot number in creeping bentgrass.
Objective of this research was to determine the effect of application rate of a slow release fertilizer (SRF) in three root media, peatmoss+vermiculite (1:1, v/v; PV), peatmoss+composted rice hall (1:1, v/v; PR), and peatmoss+composted pine bark (1:1, v/v; PB), on growth and nutrient contents of potted chrysanthemum 'Lima Honey'. All media contained polyacrylic acid sodium salt at a rate of $4.5g L^{-1}$. The fresh and dry weights at 43 days after transplanting did not show statistical differences among treatments in each root media. Elevated application rate of SRF increased fresh and dry weights at 80 days after transplanting in PV and PB media, but not in PR medium. Elevated application rates of SRF resulted in the increase of tissue phosphorus content and decrease of tissue Ca, Na, and Zn contents at both 43 and 80 days after transplanting. Elevated application rates of SRF resulted in the decrease of pH and increase of EC and concentrations of ${NO_3}^-$ and ${P_2O_5}^{3-}$, K, Ca, and Mg in the soil solution of PV and PR media. The trends of those in PR media were also similar except ${NO_3}^-$. The differences among treatments in EC at 80 days after transplanting were less significant as compared to those at 43 days after transplanting in three media.
Biological treatment of Arabian light crude oil-contaminated pebble was investigated in laboratory microcosms after supplementation with inorganic nutrients and oil-degrading microorganisms. Glass columns ($10cm{\times}20cm$) were used as microcosms and each microcosm was filled with pebbles of diameter less than 40 mm. After initial oil contamination of 2.4% (w/v), Inipol EAP-22 or slow release fertilizer (SRF) was added as inorganic nutrients and microorganisms were sprayed over pebbles. When $C_{17}$/pristane and $C_{18}$/phytane ratios were used as a marker for oil biodegradation, both ratios for microcosm supplemented with SRF and microorganisms were the lowest (below detectable range) after 92 days. Elimination of oil by abiotic processes, however, were minimal with decrease of $C_{17}$/pristane and $C_{18}$/phytane ratios from 3.55 and 2.41 to 3.06 and 1.50, respectively. The numbers of heterotrophic and oil-degrading microorganisms, and biological activity (dehydrogenase activity) corresponded to the course of biodegradation activities in all microcosms. During the whole experimental period, there was no significant nutrient deficiency only in the microcosm with SRF and microorganisms. It seemed that a continuous supply of inorganic nutrients using SRF was the most important factor for the successful performance of biological treatment in oil-contaminated pebbles.
This experiment was conducted to investigate the effect of water soluble fertilizer(WSF) and slow release fertilizer(SRF) on the growth of carnation(Dianthus caryophyllus 'Invitation') cultured in C-channel mat irrigation system. Plants grown in $0.8{\~}1.0\;g{\cdot}L^{-1}$ of WSF showed the highest quality, especially on fresh and dry weight of aerial part, leaf number, total leaf area, plant height, and branch number. All plants showed increase of growth rate around 60 days after treatment, although there was a different increase rate. Total leaf area decreased its increase rate after 90 days after treatment. Carnations supplied by WSF showed better growth and quality than SRF, and the optimum concentration range of WSF for pot-carnation was $0.8{\~}1.0\;g{\cdot}L^{-1}$ in C-channel mat irrigation system.
Slow-release fertilizers (SRF) have been used to reduce nutrient loss through increasing fertilizer efficiency and to save labor. Several SRFs were developed for rice plant in Korea, but there is few for horticultural crop plants. Two slow-release complex fertilizers, 100T and 150T, which made for controlling nitrogen release time up to 100 and 150 days, respectively, were selected for the incubation test cto evaluate nitrogen (N) release rate in soil. The N of urea selected as the control was completely released within a week after application. Sixty three and 53% of total N were released from 110T and 150T of slow release fertilizers within 8th weeks after application, respectively. For pepper cultivation CF110 and CF150, new slow-release complex fertilizer, were made of mixing 40% of conventional fertilizer and 60% of 110T and 150T, respectively, based on the amount of recommended fertilizer for pepper cultivation $(N-P_2O_5-K_2O=190-112-149\;kg\;ha^{-1})$, and were totally applied before pepper transplanting in the field as the basal fertilizer. Inorganic N $(NH_4^+-N+NO_3^--N)$ concentration in soil was higher in the CF110 treatment than in the control (NPK) at all period of pepper cultivation. In the CF150 treatment concentration of inorganic N in soil was low compared to control up to 8th weeks after transplanting. However, there was no difference in plant height and nutrient content of pepper leave between CF110 treatment and the control. In comparison, plant height was significantly lower in CF150 than the control and CF110 treatments. Around 4% of fresh pepper yield was increased in CF110 compared to the control, but it was decreased to about 2% by CF150 treatment. Conclusively, CF110 form could be recommended as a slow release fertilizer for pepper cultivation.
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