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Microprogation And Environment Conditions Affecting On Growth Of In Vitro And Ex Vitro Of A. Formosanus Hay

  • Ket, Nguyen-Van;Paek, Kee-Yoeup
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11a
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    • pp.29-30
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    • 2002
  • The goal of this research was to develop the effectiveness of in vitro culture method for A. formosanus and study the environment in vitro conditions affecting on growth. The first series of experiments were examined to investigate the response of three different basal media, MS (Murashige and Skoog, 1962), Knudson (KC; Knudson, 1946) and modified hyponex on growth and multiplication during in vitro culture. Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with BA (1 mg1$\^$-1/) or TDZ (1-2 mg1$\^$-1/). Addition of activated charcoal (1%) to the TDZ containing medium promoted rapid shoot tip proliferation (11.1 shoots per explant) but the same medium had an opposite effect resulting in poor proliferation in the nodal explants. However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 media supplemented with 2% sucrose and 0.5% activated charcoal. Using bioreactor culture for scaling up was also shown the best way for multiple shoot induction and growth of this plant. The second series of experiments was studied to investigate the effect of physical environment factors on growth of in vitro plantlets. The Anoectochilus formosanus plantlets were cultured under different air exchange rate (0.1, 0.9, 1.2h$\^$-1/), without sucrose or supplement 20g.1$\^$-1/ (photoautotrophic or photomixotrophic, respectively), and different photosynthesis photon flux (40, 80, 120 ,${\mu}$mol.m$^2$.s$\^$-1/- PPF). Under non-enrichment CO$_2$ treatment, slow growth was observed in photoautotrophical condition as compared with photomixotrophical condition on shoot height, fresh weigh and dry weight parameters; High air exchange (1.2.h-l) was found to be inadequate for plant growth in photomixotrophical condition. On the contrary, under CO$_2$, enrichment treatment, the plant growth parameters were sharply (visibly) improved on photoautotrophic treatments, especially on the treatment with air exchange rate of 0.9.h-1. The growth of plant in photoautotrophic condition was not inferior compared with photomixotrophic, and the best growth of plantlet was observed in treatment with low air exchange rate (0.9.h-1). Raising the PPF level from 80 to 120${\mu}$mol.m$\^$-2/.s$\^$-1/ decreased the plant height, particularly at 120${\mu}$mol.m$\^$-2/.s$\^$-1/ in photoautotrophic condition, fresh weight and dry weight declined noticeably. At the PPF of 120${\mu}$mol.m$\^$-2/,s$\^$-1/, chlorophyll contents lowed compared to those grown under low PPF but time courses of net photosynthesis rate was decreased noticeably. Light quality mainly affected morphological variables, changes of light quality also positively affected biomass production via changes in leaf area, stem elongation, chlorophyll content. Plant biomass was reduced when A. formosanus were grown under red LEDs in the absence of blue wavelengths compare to plants grown under supplemental blue light or under fluorescent light. Stem elongation was observed under red and blue light in the present experiment. Smaller leaf area has found under blue light than with other lighting treatments. Chlorophyll degradation was more pronounced in red and blue light compared with white light or red plus blue light which consequent affected the photosynthetic capacity of the plant. The third series of experiment were studied to investigate the effect of physical environment factors on growth of ex vitro plants including photosynthesis photon flux (PPF), light quality, growing substrates, electrical conductivity (EC) and humidity conditions. In the present experiments, response of plant on PPF and light quality was similar in vitro plants under photosynthesis photon flux 40${\mu}$mol.m,$\^$-2/.s$\^$-1/ and white light or blue plus red lights were the best growth. Substrates testing results were indicated cocopeat or peat moss were good substrates for A. formosanus growth under the greenhouse conditions. In case of A. formosanus plants, EC is generally maintained in the range 0.7 to 1.5 dS.m-1 was shown best results in growth of this plant. Keeping high humidity over 70% under low radiation enhanced growth rate and mass production.

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The Effects of Visual and Auditory Stimulation on the Ability to Perform Exercise (시각과 청각 자극이 운동수행능력에 미치는 영향)

  • Park, Kwang-Hyun;Kim, Yu-Min;Kim, Hyun-A;Seo, Han-Bit;Son, Won-Bin;Song, Eun-Ji;Shin, Su-Jin;Ahn, Ha-Rim;Lee, Choong-Jung;Cho, Min-Ok;Kim, Min-Hee
    • PNF and Movement
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    • v.14 no.2
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    • pp.121-130
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    • 2016
  • Purpose: The purpose of this study was to investigate the effects of visual and auditory stimulation on the ability to perform exercise. Methods: One hundred twenty subjects were randomly divided into four groups (Green light and Fast tempo music, GF; Green light and Slow tempo music, GS; Red light and Fast tempo music, RF; and Red light and Slow tempo music, RS). One of either two visual stimuli or one of two auditory stimuli were applied to each group. The experiment was conducted randomly twice in two environments: one had visual and auditory stimuli and one had no stimulation. Muscle strength, grip, endurance, quickness, agility, concentration, and balance were measured to determine the ability to perform exercise. Results: Significant differences were found in the muscle strength of the participants who were exposed to the auditory factor and the interaction of visual and auditory factors. In endurance, significant differences were found in all of the factors: visual, auditory, and the interaction of visual and auditory. In quickness, agility, and balance ability there were significant differences in the visual factor. In concentration, there was a significant difference in the auditory factor. Conclusion: Visual stimuli, auditory stimuli, and their interaction influenced the ability to perform exercise. These facts imply that providing the proper environmental stimulation is important to increase the ability to perform during exercise.

VARIABLE STARS IN THE REGION OF THE OPEN CLUSTER NGC 457 (산개성단 NGC 457 영역의 변광성)

  • Jeon, Young-Beom;Park, Yoon-Ho;Lee, Sang-Min
    • Publications of The Korean Astronomical Society
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    • v.32 no.3
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    • pp.421-438
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    • 2017
  • Through the short-period variability survey program, we obtained time-series BV CCD images for $1.5^{\circ}{\times}1.0^{\circ}$ region around the young open cluster NGC 457. As a result, we have detected 61 variable stars including 31 new ones after checking light curves of all stars by eyes. The 61 variable stars were included 14 ${\delta}$ Scuti variable stars, a ${\beta}$ Cephei variable star, 10 variable Be and slowly pulsating B stars, 13 eclipsing binary stars, 21 semi-long periodic or slow irregular variables and an RR Lyrae variable star, respectively. Many variable B-type stars were known through a well-defined zero-age main sequence to the ${\beta}$ Cepheid region of NGC 457. Most of the variable B-type stars found this paper were known variable stars. But, 11 out of 14 ${\delta}$ Scuti variable stars were newly discovered. The new variable stars except for ${\delta}$ Scuti stars were 4 variable B-type stars, 5 eclipsing binaries and 11 semi-long periodic or slow irregular variables. We have performed frequency analysis for all ${\delta}$ Scuti stars, a ${\beta}$ Cepheid star and an RR Lyrae star.

Stress Corrosion Cracking of High Strength Al-Zn-Mg-Cu Aluminum Alloy with Different Compositions (고강도 Al-Zn-Mg-Cu 합금에서 조성에 따른 응력부식균열 특성)

  • Kim, Jun-Tak;Kim, Sang-Ho
    • Journal of the Korean institute of surface engineering
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    • v.41 no.3
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    • pp.109-113
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    • 2008
  • High strength 7xxx series Al-Zn-Mg alloy have been investigated for using light weight automotive parts especially for bump back beam. The composition of commercial 7xxx aluminum has the Zn/Mg ratio about 3 and Cu over 2 wt%, but this composition isn't adequate for appling to automotive bump back beam due to its high resistance to extrusion and bad weldability. In this study the Zn/Mg ratio was increased for better extrusion and Cu content was reduced for better welding. With this new composition we investigated the effect of composition on the resistivity against stress corrosion cracking. As the Zn/Mg ratio is increased fracture energy obtained by slow strain rate test was decreased, which means degradation of SCC resistance. While the fracture energy was increased with Cu contents although it is below 1%, which means improvement of SCC resistance. These effects of composition change on the SCC resistivity were identified by observing the fracture surface and crack propagation.

Slow Mass Movement on a Subalpine Slope of Mount Halla, Jeju Island (한라산 아고산대에서의 사면 물질 이동)

  • Kim, Tae-Ho
    • Journal of the Korean Geographical Society
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    • v.45 no.3
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    • pp.375-389
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    • 2010
  • In order to investigate the rates and factors of slow mass movement on a subalpine slope of Mount Halla, two painted stone lines were monitored in a bare patch at 1,710 m a.s.l. The mean movement of surface gravels is 58.2 cm, equivalent to 0.24 cm/day. However, the rates of movement vary with seasons. Compared with 0.05 cm/day of a non-frozen season, a frozen season shows 0.3 cm/day. It implies that the movement of surface gravels could be largely controlled by periglacial processes during a frozen season. In particular, frost creep including needle ice creep plays a main role in the movement of gravels under the thermal and soil conditions which are favorable for needle ice development. Since line II is located at a steeper slope than line I, the movement of line II was always larger than that of line I. However, slope gradient is not the most dominant factor contributing the movement of gravels, which can be interrupted by downslope big gravels and vegetation patches. The size and specific weight of gravels also can influence the movement of gravels. Porous and light scoriae result in relatively quick movement of gravels on the subalpine slope of Mount Halla.

A Study on Link Travel Time Prediction by Short Term Simulation Based on CA (CA모형을 이용한 단기 구간통행시간 예측에 관한 연구)

  • 이승재;장현호
    • Journal of Korean Society of Transportation
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    • v.21 no.1
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    • pp.91-102
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    • 2003
  • There are two goals in this paper. The one is development of existing CA(Cellular Automata) model to explain more realistic deceleration process to stop. The other is the application of the updated CA model to forecasting simulation to predict short term link travel time that takes a key rule in finding the shortest path of route guidance system of ITS. Car following theory of CA models don't makes not response to leading vehicle's velocity but gap or distance between leading vehicles and following vehicles. So a following vehicle running at free flow speed must meet steeply sudden deceleration to avoid back collision within unrealistic braking distance. To tackle above unrealistic deceleration rule, “Slow-to-stop” rule is integrated into NaSch model. For application to interrupted traffic flow, this paper applies “Slow-to-stop” rule to both normal traffic light and random traffic light. And vehicle packet method is used to simulate a large-scale network on the desktop. Generally, time series data analysis methods such as neural network, ARIMA, and Kalman filtering are used for short term link travel time prediction that is crucial to find an optimal dynamic shortest path. But those methods have time-lag problems and are hard to capture traffic flow mechanism such as spill over and spill back etc. To address above problems. the CA model built in this study is used for forecasting simulation to predict short term link travel time in Kangnam district network And it's turned out that short term prediction simulation method generates novel results, taking a crack of time lag problems and considering interrupted traffic flow mechanism.

Photocatalytic Activity of Hierarchical N doped TiO2 Nanostructures

  • Naik, Brundabana;Kim, Sun Mi;Jung, Chan Ho;Park, Jeong Young
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.669-669
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    • 2013
  • Hierarchical N doped TiO2 nanostructured catalyst with micro, meso and macro porosity have been synthesized by a facile self-formation route using ammonia and titanium isopropoxide precursor. The samples were calcined in different calcination temperature ranging from $300^{\circ}C$ to $800^{\circ}C$ at slow heating rate ($5^{\circ}C$/min) and designated as NHPT-300 to NHPT-800. $TiO_2$ nanostructured catalyst have been characterized by physico-chemical and spectroscopy methods to explore the structural, electronic and optical properties. UV-Vis diffuse reflectance spectra confirmed the red shift and band gap narrowing due to the doping of N species in TiO2 nanoporous catalyst. Hierarchical macro porosity with fibrous channel patterning was observed (confirmed from FESEM) and well preserved even after calcination at $800^{\circ}C$, indicating the thermal stability. BET results showed that micro and mesoporosity was lost after $500^{\circ}C$ calcination. The photocatalytic activity has been evaluated for methanol oxidation to formaldehyde in visible light. The enhanced photocatalytic activity is attributed to combined synergetic effect of N doping for visible light absorption, micro and mesoporosity for increase of effective surface area and light harvestation, and hierarchical macroporous fibrous structure for multiple reflection and effective charge transfer.

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Differential expression of the enzymes regulating myosin light chain phosphorylation are responsible for the slower relaxation of pulmonary artery than mesenteric artery in rats

  • Seung Beom Oh;Suhan Cho;Hyun Jong Kim;Sung Joon Kim
    • The Korean Journal of Physiology and Pharmacology
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    • v.28 no.1
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    • pp.49-57
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    • 2024
  • While arterial tone is generally determined by the phosphorylation of Ser19 in myosin light chain (p-MLC2), Thr18/Ser19 diphosphorylation of MLC2 (pp-MLC2) has been suggested to hinder the relaxation of smooth muscle. In a dual-wire myography of rodent pulmonary artery (PA) and mesenteric artery (MA), we noticed significantly slower relaxation in PA than in MA after 80 mM KCl-induced condition (80K-contraction). Thus, we investigated the MLC2 phosphorylation and the expression levels of its regulatory enzymes; soluble guanylate cyclase (sGC), Rho-A dependent kinase (ROCK) and myosin light chain phosphatase target regulatory subunit (MYPT1). Immunoblotting showed higher sGC-α and ROCK2 in PA than MA, while sGC-β and MYPT1 levels were higher in MA than in PA. Interestingly, the level of pp-MLC2 was higher in PA than in MA without stimulation. In the 80K-contraction state, the levels of p-MLC2 and pp-MLC2 were commonly increased. Treatment with the ROCK inhibitor (Y27632, 10 µM) reversed the higher pp-MLC2 in PA. In the myography study, pharmacological inhibition of sGC (ODQ, 10 µM) slowed relaxation during washout, which was more pronounced in PA than in MA. The simultaneous treatment of Y27632 and ODQ reversed the impaired relaxation in PA and MA. Although treatment of PA with Y27632 alone could increase the rate of relaxation, it was still slower than that of MA without Y27632 treatment. Taken together, we suggest that the higher ROCK and lower MYPT in PA would have induced the higher level of MLC2 phosphorylation, which is responsible for the characteristic slow relaxation in PA.

Tri-branched tri-anchoring organic dye for Visible light-responsive dye-sensitized photoelectrochemical water-splitting cells (염료감응형 광전기화학 물분해 전지용 Tri-branched tri-anchoring organic dye 개발)

  • Park, Jeong-Hyun;Kim, Jae-Hong;Ahn, Kwang-Soon
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.87-87
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    • 2010
  • Photoelectrochemical (PEC) systems are promising methods of producing H2 gas using solar energy in an aqueous solution. The photoelectrochemical properties of numerous metal oxides have been studied. Among them, the PEC systems based on TiO2 have been extensively studied. However, the drawback of a PEC system with TiO2 is that only ultraviolet (UV) light can be absorbed because of its large band gap (3.2 - 3.4 eV). Two approaches have been introduced in order to use PEC cells in the visible light region. The first method includes doping impurities, such as nitrogen, into TiO2, and this technique has been extensively studied in an attempt to narrow the band gap. In comparison, research on the second method, which includes visible light water splitting in molecular photosystems, has been slow. Mallouk et al. recently developed electrochemical water-splitting cells using the Ru(II) complex as the visible light photosensitizer. the dye-sensitized PEC cell consisted of a dye-sensitized TiO2 layer, a Pt counter electrode, and an aqueous solution between them. Under a visible light (< 3 eV) illumination, only the dye molecule absorbed the light and became excited because TiO2 had the wide band gap. The light absorption of the dye was followed by the transfer of an electron from the excited state (S*) of the dye to the conduction band (CB) of TiO2 and its subsequent transfer to the transparent conducting oxide (TCO). The electrons moved through the wire to the Pt, where the water reduction (or H2 evolution) occurred. The oxidized dye molecules caused the water oxidation because their HOMO level was below the H2O/O2 level. Organic dyes have been developed as metal-free alternatives to the Ru(II) complexes because of their tunable optical and electronic properties and low-cost manufacturing. Recently, organic dye molecules containing multi-branched, multi-anchoring groups have received a great deal of interest. In this work, tri-branched tri-anchoring organic dyes (Dye 2) were designed and applied to visible light water-splitting cells based on dye-sensitized TiO2 electrodes. Dye 2 had a molecular structure containing one donor (D) and three acceptor (A) groups, and each ended with an anchoring functionality. In comparison, mono-anchoring dyes (Dye 1) were also synthesized. The PEC response of the Dye 2-sensitized TiO2 film was much better than the Dye 1-sensitized or unsensitized TiO2 films.

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Multiple FBG Sensor System Using Code Division Multiple Access (코드분할 다중화 방식을 이용한 다중 광섬유 브래그 격자 센서 시스템)

  • Ryu, Hyung-Don;Lee, Ho-Joon
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.38 no.8
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    • pp.27-33
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    • 2001
  • The performance of the ordinary Fiber Bragg Grating(FBG) sensor strain measurement system, which uses Fabry-Perot filter for scanning wavelength, has limitation for application because of hysteresis characteristics of PZT element in the filter, slow scan rate of the filter and the high cost of system. We proposed and experimented a multiple FBG sensor system using light emitting diode(LED) as a light source and adapting Code Division Multiplexing(CDM) method to separating out individual sensor signal. Output signals for a applied static and dynamic strain and crosstalk levels between sensor signals were measured. The price of the system is very loss and the response speed is very fast. Crosstalk levels between sensor signals below - 30 dB were demonstrated.

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