• 대한의용생체공학회:의공학회지
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• 제16권3호
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• pp.279-288
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• 1995

Ultrasonic Doppler systems for the purpose of estimating blood flow velocity, blood flow volume, and flow imaging are commonly used due to advantages of non-invasive and real time observation. Specially, the technical developments of color flow mapping (2-D Doppler) systems have made a relatively rapid progress. However, the 2-D Doppler systems have several problems, such as the range ambiguity, low signal to noise ratio, and slow frame rate. The slow frame rate problem is resolved by using the spatial average which is a method to acquire more data samples for mean frequency estimation. In this paper, spatial average method using the 2nd order sampling instead of quadrature sampling is proposed. The experimental results show that the proposed methods have good performance and easy application to the color flow mapping system.

• 한국수정란이식학회지
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• 제18권1호
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• pp.43-50
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• 2003

정자의 동결보존을 위한 새로운 기술개발 목적은 동결과정에서 최소한의 손상으로, 응해 후 최대한 높은 활력도의 정자를 얻는 것이다 정자가 난자와 수정하기 위해서는 적당한 생존성과 운동성을 유지해야 하는데, 가장 일반적인 방법으로는 정자의 진진 운동성과 첨체의 정상 여부 및 형태 검사방법 등이 있다 본 연구는 사람 정액을 동결보존 할 때 semi-programmable freezer를 이용한 완만동결 방법과, 액체질소의 vapor를 이용한 급속동결 방법이 응해 후 정자의 운동양상과 생존율 및 형태에 미치는 영향을 알아보기 위해 실시하였다. 동결-응해 후 정자의 MOT, VCL, VSL, VAP는 각각 급속 동결방법에서 47.40$\pm$20.06%, 38.12$\pm$15.58 $\mu$m/s, 28.19$\pm$14.10 $\mu$m/s, 33.64$\pm$15.15 $\mu$m/s로 완만 동결방법인 43.39$\pm$18.79%, 33.91$\pm$13.50 $\mu$m/s, 19.98$\pm$10.88 $\mu$m/s, 24.60$\pm$11.72 $\mu$m/s보다 유의적으로 높았으나(p<0.05), LIN은 완만동결 방법이 34.64$\pm$11.36으로 급속동결 방법인 28.83$\pm$10.35보다 더 좋은 성적을 나타내었다(p<0.05). 고 활력정자를 나타내는 HYP 역시 급속동결 방법에서 2.77$\pm$2.71%로 완만동결 방법의 1.33$\pm$1.57%보다 유의적으로 높게 나타났다(p<0.05). 그러나 정자의 운동양태를 나타내는 MAD, WOB, DNC, DNM에 있어서는 완만동결 방법이 급속동결 방법에 비해 조금 더 좋은 성적을 보였으나 유의적인 차이는 없었다. 동결-응해 후 정자의 생존율 및 정상형태의 정자는 완만동결 방법이 각각 62$\pm$2.1%, 44$\pm$8.3%, 급속동결 방법은 60$\pm$2.2%, 46$\pm$7.7%로 유의적인 차이가 없이 비슷한 결과를 보였다. 따라서 사람 정액의 동결 보존 시 많은 시간과 고가의 장비가 필요한 완만동결 방법보다는 짧을시간동안 액체 질소만으로 간단히 시행할 수 있는 급속동결 방법이 더 효과적이라고 사료된다.

• 한국수정란이식학회지
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• 제11권3호
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• pp.271-281
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• 1996

The effect of superovulation (PMSG, FSH) on the ovarian response of matured cows were tested. The survival rates of bovine embryos and ovarian oocytes frozen by slow, rapid freezing and vitrification were investigated. A total of 15 heads of cow were devided into 3 groups by injection dose of GTH (PSMG, FSH). Each group was superrovulated with injections of 2, 500, 3, OOOJU PSMG and 40mg FSH followed by injection of 30mg PGF2a. Embryos were non-surgically recovered from superovulated cows 6~7days after estrus. The recovered embryos were frozen in 10% glycerol + 10% sucrose by slow and rapid freezing. Ovarian oncytes were frozen in 20% g]ycerol+l0% ethylene glycerol + 30% Ficol + 10% sucrose by vitrification and the survival of frozen embryos and ovarian oncytes were judged by FDA-test. The results are summarized as follows; 1. Estrus after the injection of 2500, 3000 I.U. PMSG and 4Omg FSH were 32.8, 35.0 and 43.4 and the duration of estrus were 18.6, 18.8 and 22.4 hours respectively. 2. The average sizes of the left ovaries were 5.4cm (2, 500 IU PMSG), 5.1cm (3, OOOIU PMSG) and 6.4cm (FSH), and the right were 6.2cm (2, 5001U PMSG), 5.7cm (3, OOOIU PMSG) and 7.&m (FSH) respectively. There were significant differences in the right overies among treatments (P<0.05). 3. The average number of ovarian follicles in the left ovaries were 4.8 (2, 500 IU PMSG), 5.2(3, 000 IU PMSG) and 7.8 (FSH) respectively. There were significant difference in the right ovaries among treatments (P<0.05). 4. In the average numbers of ovulation points in the left ovaries were 3.0 (2, 5001U PMSG), 3.2 (3, OOOIU PMSG) and 4.4 (FSH) respectively, and the right were 7.2 (2, 5001U PMSG), 7.8(3, 000IU PMSG) and 11.4 (FSH). There were significant differences in the right ovaries among treatments (P<0.05). 5. The numbers of the recovered embryos were 20 (2, 5001U PMSG), 19 (3, 000 IU PMSG) and 21 (FSH) respectively, and oncytes and degenerted oncytes were 6.5 and 11.0 Estrus periods of post parturation were 52.4days (2, 5001U PMSG), 69.8days (3, OOOIU PMSG) and 62.4days (FSH) respectively. 6. The FDA score of cow morulae frozen by slow freezing, sernirapid frezing and vitrified freezing was higher in slow (3.1) and vitrified freezing (3.0) than that in semirapid freezing (1.28). The FDA-scores of cow, pig and rabbit ovarian oocytes frozen in 20% glycerol + 10% ethylene glycol + 30% Ficoll + 10% sucrose by vitrification were higher in cows (3.3) than both in pigs (2.6) and rabbits (2.3).

• Agricultural and Biosystems Engineering
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• 제3권1호
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• pp.10-17
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• 2002

Quality attributes of apple are greatly affected by the cooling rate and environmental conditions during storage. Studies were conducted to evaluate the effect of cooling rate on different quality attributes of apple. The effect of $O_2$ pulldown rate of the CA chamber on the quality of apple was also determined. Two methods were used viz. conventional CA procedure and rapid CA procedure. Apples stored by medium and slow cooling methods lost its flesh firmness significantly from an initial level of 4.55 kg to 2.83 kg and 2.27 kg, respectively on 35 days after storage whereas, in rapid cooling, the firmness level changed from 4.55 kg to 3.20 kg on 35 days after storage. At the end of 35 days of storage, titratable acidity decreased insignificantly from an initial value of 0.24l% to 0.239% in the case of rapid CA whereas in the case of conventional CA it dropped significantly to 0.215% from its initial level. The initial flesh firmness of 4.55 kg also changed significantly to 4.05 kg on 35 days after storage in conventional CA whereas in rapid CA it changed to 4.36 kg, which was found to be non-significant at 5% level of significance. Total soluble solids increased from an initial level of $12.43^{\circ}$Bx to $12.60^{\circ}C.$ Bx on 35 days after storage in rapid CA whereas it increased to $13.07^{\circ}$ Bx in conventional CA. Ascorbic acid content of apple juice decreased insignificantly from 6.67 mg/100 mL to 5.87 mg/100 mL on 35 days after storage in rapid CA whereas in conventional CA, it decreased significantly to 5.27 mg/100 mL from its initial level.

• 한국수정란이식학회지
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• 제29권3호
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• pp.235-240
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• 2014

생쥐 2세포기, 4세포기, 8세포기를 각 발생 단계에서 채취하여 동결 보호제를 첨가한 서로 다른 배양액에서 배양하고, 배아의 동결 보존 및 융해 시 급속 처리와 저속 처리 단계로 비교하여 이들 조건이 배아의 생존과 발현에 미치는 영향을 조사하여 다음의 결과를 얻었다. 동결 보호제로 처리하여 배양액을 달리한 경우, 급속 단계에서는 모든 배양액에서 비슷한 발생율을 보였고, 저속단계의 4세포기와 8세포기는 D-PBS에서 높은 발생율을 보였다(P<0.05, P<0.01). 배아의 발생 시기에 따른 동결 보존 후, 발생율은 2, 4, 8세포기로 넘어갈수록 발생율의 증가를 보여 8세포기에서 발생율이 가장 높았다(P<0.01). 동결 보호제의 처리단계에 따른 발생율은 2세포기의 급속 단계에서는 유사하였으나, 4세포기와 8세포기는 저속단계에서 높은 발생율을 보였으며(P<0.05), 특히 8세포기에서 가장 높았다(P<0.01).

• 한국가축번식학회지
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• 제8권1호
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• pp.22-28
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• 1984

This experiment was carried out to investigate effects of DMSO or ethylene glycol as a cryopotectant and of freezing methods on survival rate of forozen-thawed rat 2-cell embryos by morphological observation. 2-cell embryos were recovered from oviducts of Sprague Dawley females mated with males of same strain on day 2 of pregnancy after inducing superovulation by intrapertioneal injection of PMSG and HCG. In slow freezing and thawing groups, embryos were frozen to -79$^{\circ}C$ or -196$^{\circ}C$ in a glass test tube containing 0.2ml PBI with 1.5M DMSO or 1.2M ethylene glycol at a rate of 0.3-1.0C/min. and thawed slowly. When samples were frozen to -79$^{\circ}C$, higher survival rate was obtained in the medium containing DMSO (43.9%) than ethylene glycol (41%). And similar result was obtained (32.5% in DMSO vs. 31.4% in ethylene glycol) when samples were frozen. In rapid freezing and thawing groups, embryos were frozen to -79 or -196$^{\circ}C$ in a glass test tube containing 0.2ml of PBI with 1.5M DMSO or 1.2M ethylene glycol by rapid cooling, and thawed rapidly. When samples were frozen to -79$^{\circ}C$, 1.5M DMSO (13.2%) was more effective than 1.2M ethylene glycol (6.1%). When the storage temperature was -196$^{\circ}C$, survival rates were 9.8% in 1.5M and 5.4% in 1.2M ethylene glycol.

• 사상체질의학회지
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• 제19권3호
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• pp.126-143
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• 2007

1. Objectives The purpose of this study is to find out the grade of practical use, the important element and the significant characteristics of Sasangin' s face, voice, skin and pulse diagnostic impression, in Sasang constitutional clinical diagnosis. 2. Methods We analysed the survey data about Sasangin' s face, voice, skin and pulse diagnostic impression, drawn up by specialist in Sasang constitutional medicine. 3. Results and Conclusions (1) In the application degree of face feature, the case which it was applied with 20-40% and 40-60% were 16 people(43.2%) respectively. In voice, the case applied with 0-20% was 19 people(51.4%), in skin, 0-20% and 20-40% were 14 people(37.8%) respectively and in pulse diagnosis, 0-20% were 25 people(73.0%). (2) In constitutional diagnosis, the important element of face were 'frontal whole shape', 'whole impression' and 'size and shape of eye, ear, mose and mouth', the important element of voice 'speed of speech', 'purity and impurity' and 'pitch', the important element of skin 'thickness', 'feel of touch' and 'size of skin pores' and the important element of pulse diagnosis 'speed of pulse', 'sinking and floating' and 'weakness and firmness'. (3) The important face characteristics of Taeyangin were 'bright eye', 'broad forehead' and 'strong impression', Soyangin 'protruding forehead', 'thin and small lips', 'narrowing and sharp chin', Taeumin 'thick lips', 'flat face', 'large eye, nose, ear and mouth' and Soeumin 'long and slender face', 'downward slanting eyes' and 'small eye, nose, ear and mouth', The important voice characteristics of Taeyangin were 'loud' and 'clear', Soyangin 'rapid' and 'high-pitched tone', Taeumin 'chick', 'slow' and 'low-pitched tone' and Soeumin 'small and feeble' and 'slow'. The important skin characteristics of Taeyangin were 'thin' and 'white', Soyangin 'thin', 'smooth' and 'elastic', Taeumin 'thick', 'large skin-pore', 'coarse' and Soeumin 'soft', 'thin' and 'subtle skin-pore'. The important pulse characteristics of Taeyangin were 'rapid' and 'large', Soyangin 'rapid' and 'floating', Taeumin 'tense', 'long' and 'solid' and Soeumin 'fine', 'weak' and 'slow'.

• Journal of Acupuncture Research
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• 제29권5호
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• pp.151-158
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• 2012

Objectives : We hypothesized that reinforcing-reducing manipulations produce different effects by quantity of stimulation, and examined whether the difference is statistically significant or not. Methods : The study was conducted to the students of College of Oriental Medicine, Dongguk University. We needled into $ST_{36}$ and applied 6 kinds of basic reinforcing-reducing manipulations in Huang di nei jing by randomized sequence. And we had the students to fill up the questionnaire in form of visual analog scale(VAS). We examined whether significant differences in VAS existed between reinforcing-reducing manipulations(by t-test). And we examined whether significant differences in VDRRT(VAS difference between reinforcing-reducing technique) values existed along the gender, body mass index(BMI), age(by t-test), and psychological sensitivity(by ANOVA). Results : 1. The total stimulation quantity from reducing technique was significantly higher than that from reinforcing technique. Individually VAS values of reducing techniques were significantly higher in rotation, slow-rapid, lift-thrust, respiration and open-close manipulation. 2. The highest stimulation quantity was from rotation manipulation. And the highest VDRRT value was from slow-rapid manipulation. 3. When VDRRT values were analyzed by gender, age, BMI, and psychological sensitivity, VDRRT was significantly different by BMI in rotation manipulation, and by psychological sensitivity in rotation, lift-thrust, and slow-rapid manipulation. Conclusions : We could verify that reinforcing-reducing techniques induce different therapeutic effects by different quantities of stimulations. It suggests that reinforcing-reducing techniques could be applied universally, regardless of gender or age. Further studies are needed to consider control group, or patient groups in characteristic conditions.

• 한국철도학회논문집
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• 제8권6호
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• pp.610-616
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• 2005

The Incheon International Airport Express(I'Rex) is the railroad that connects Incheon International Airport, Gimpo Airport and Seoul Station. I'Rex is now under construction, and it will be connected to Gimpo Airport by 2007, to Seoul Station by 2010. However, there has been no discussion on how th connect different routes of railway Systems. Therefore it seems likely that rail passenger demand will be lower than that of limousine bus. Therefor, this paper proposes 4 effective connecting methods for I'Rex - (1) Shuttle transport, (2) Short-distance connecting transport (Inside I'Rex, Seoul Subway Line 9, 'Han-River line') (3) Mid-distance connecting transport (Jung-ang, Tae-baek, Young-dong line) (4) Long-distance connecting transport (Korean Train Express (Korean high speed railroad)) These methods can increase the connectivity of I'Rex with existing railroads and the competition power of I'Rex against a highway.

• Molecules and Cells
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• 제37권3호
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• pp.202-212
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• 2014

ClC-1 is a member of a large family of voltage-gated chloride channels, abundantly expressed in human skeletal muscle. Mutations in ClC-1 are associated with myotonia congenita (MC) and result in loss of regulation of membrane excitability in skeletal muscle. We studied the electrophysiological characteristics of six mutants found among Korean MC patients, using patch clamp methods in HEK293 cells. Here, we found that the autosomal dominant mutants S189C and P480S displayed reduced chloride conductances compared to WT. Autosomal recessive mutant M128I did not show a typical rapid deactivation of Cl- currents. While sporadic mutant G523D displayed sustained activation of $Cl^-$ currents in the whole cell traces, the other sporadic mutants, M373L and M609K, demonstrated rapid deactivations. $V_{1/2}$ of these mutants was shifted to more depolarizing potentials. In order to identify potential effects on gating processes, slow and fast gating was analyzed for each mutant. We show that slow gating of the mutants tends to be shifted toward more positive potentials in comparison to WT. Collectively, these six mutants found among Korean patients demonstrated modifications of channel gating behaviors and reduced chloride conductances that likely contribute to the physiologic changes of MC.