• Korean Journal of Veterinary Service
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• v.33 no.1
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• pp.29-35
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• 2010

This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with $1{\times}10^9$ CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis ($5.0{\times}10^9$ CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 ($4.4{\times}10^{10}$ CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.

• Preventive Nutrition and Food Science
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• v.8 no.4
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• pp.390-394
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• 2003

In the post-genome period, the technique for identifying gene expression has been progressed to high throughput screening. In the field of molecular nutrition, the use of screening techniques to clarify molecular function of specific nutrients would be very advantageous. In this study, we have evaluated Zn-regulated gene expression in Zn-deficient, homocystein-treated EA.hy926 cells, using cDNA microarray, which can be used to screen the expression of many genes simultaneously. The information obtained can be used for preliminary assessment of molecular and signaling events modulated by Zn under pro-atherogenic conditions. EA.hy926 cells derived from human umbilical vein endothelial cells were cultured in Zn-adequate (control, 15 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) Dulbecco's MEM media under high homocysteine level (100 $\mu$M) for 3 days of post-confluency. Cells were harvested and RNA was extracted. Total RNA was reverse-transcribed and the synthesized cDNA was labeled with Cy3 or Cy5. Fluorescent labeled cDNA probe was applied to microarray slides for hybridization, and the slide was then scanned using a fluorescence scanner. The expression of seven genes was found to be significantly decreased, and one significantly increased, in response to treatment of EA.hy926 cells with Zn-deficient medium, compared with Zn-supplemented medium. The upregulated genes were oncogenes and tumor suppressor genes, cell cycle-related genes and transporter genes. The down-regulated gene was RelB, a component of the NF-kappaB complex of transcription factors. The results of this study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, namely Zn. Furthur study, using tailored-cDNA array and vascular endothelial cell lines, would be beneficial to clarify the molecular function of Zn in atherosclerosis, more in detail.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.6
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• pp.1025-1029
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• 2000

This study was conducted to investigate whether a DNA comet assay could be applied for identifying irradiated pork and beef. Pork and beef were irradiated with Co-60 gamma rays at 0.1, 0.3, 0.5, 0.7 and 1.0 kGy, and stored in a freezer Cells separated from the samples were embedded in agarose gel on a slide, dissolved in a lysis solution, and electrophoresed at 2 V/cm for 2.0 min by horizontal electrophoesis. The cells were then stained with a silver staining in order to visualize the DNA using a micro-scope. The DNA fragments of the irradiated cells stretched or migrated out of the cells and formed tails towards the anode, giving the appearance of comets, while unirradiated cells formed very short or no tails. The distance of DNA migration increased with irradiation dose. Since the statistical analysis showed a significant correlation between tail length and irradiation dose, a DNA comet assay could provide not only identification but also estimation of the irradiation dose for irradiated beef and pork.

• Clinical and Experimental Pediatrics
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• v.63 no.6
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• pp.232-236
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• 2020

Background: Children today get access to smartphones at an early age. However, their ability to use mobile apps has not yet been studied in detail. Purpose: This study aimed to assess the ability of children aged 2-8 years to perform touchscreen gestures and follow prompting techniques, i.e., ways apps provide instructions on how to use them. Methods: We developed one mobile app to test the ability of children to perform various touchscreen gestures and another mobile app to test their ability to follow various prompting techniques. We used these apps in this study of 90 children in a kindergarten and a primary school in New Delhi in July 2019. We noted the touchscreen gestures that the children could perform and the most sophisticated prompting technique that they could follow. Results: Two- and 3-year-old children could not follow any prompting technique and only a minority (27%) could tap the touchscreen at an intended place. Four- to 6-year-old children could perform simple gestures like a tap and slide (57%) and follow instructions provided through animation (63%). Seven- and 8-year-old children could perform more sophisticated gestures like dragging and dropping (30%) and follow instructions provided in audio and video formats (34%). We observed a significant difference between the number of touchscreen gestures that the children could perform and the number of prompting techniques that they could follow (F=544.0407, P<0.05). No significant difference was observed in the performance of female versus male children (P>0.05). Conclusion: Children gradually learn to use mobile apps beginning at 2 years of age. They become comfortable performing single-finger gestures and following nontextual prompting techniques by 8 years of age. We recommend that these results be considered in the development of mobile apps for children.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.1
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• pp.99-109
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• 2015

In the construction industry, the interest for recycling aggregates is rising as more people demand for alternatives due to lack of supply of natural aggregates and environmental problems. However, in order for recycled aggregates to be used in infrastructures, stability and other factors need to be verified. Therefore, the objective of this study is to analyze the sensitivity of soil properties to secure slope safety according to various heights of embankment when bottom ash and dredged soil mixture is applied in the embankment. In most cases, all heights were safe for the slide for the embankment whether the water level is full or sudden draw down. The result of the sensitivity analysis revealed that the unit weight of embankments is highest among all factors to be considered. However, the sensitivity of the unit weight became smaller and the sensitivity of the friction angle of embankments increased with the height of embankments. The sensitivity of factors of core materials is very small because the core has weaker physical properties than those of the embankment. The effect of the height for each factor is different for each slope and water levels. The sensitivity of the unit weight of embankments is most affected when the height is 60m in the upstream slope. To conclude, bottom ash and dredged soil mixture can be applied in the embankment and different factors must be considered in different scale because the sensitivity depends highly on the height of embankments.

• Journal of the Korean Professional Engineers Association
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• v.16 no.3
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• pp.41-53
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• 1983

The Dangri Crushed Stone Quarry is located in Dangridong, Busan City, and around the estuary of the Nagdong River. The quarry is considered to be a very promised one in the urban area from the standpoints of the assessment of the aggregate rank, the environmental impacts and the transportation distance. The crushed stones for aggregate of the quarry marks the higher rank in the gravity, the absorption ratio, the abrasion ratio, and the stability in comparison with the JISA 5005. The basement vibrations of the residential section in the vicinity of the quarry, which are arised by the millisecond blasting at the quarry site using the gelatin dynamites less than 39kg in weight, are measured to assess the vibration nuisance. The values of acceleration and the magnitudes are less than eight gals and O on the Richter scale respectively, the vibration nuisance thus can be ignored in such scales of the experiments. The traffic vibrations of the residential section are slightly susceptible. In the experiments, the traffic vibrations appears to be sensibler to the basement than the explosion vibration. The explosion noises in the experiments are not checked not only on the RION Sound Level Meter but also to our ears. The values of traffic noises also are in the safety values of the noise nuisance. The crush dust suspends in the air toward the upper valley in the opposite side of the residential area because of the influences of the sea breeze and the valley wind in the daytime, and the monsoon and the topographic disposition. the dust nuisance thus would not be remained in problem. The quarry is operated in the daytime only. The traffic dust in the residential area will be reduced by the faultless pavement and the careful driving. The elaborate survey on the ridges and peaks surrounded the quarry is recommended to prevent in advance the accidents of the rock slide. Moreover, it is required to make an advisory committee to develop the industry and to save the techniques. The most important matter is the accomodation between the attitude of the enterprising man for the social responsibility to the public nuisance and the cooperative spirit of the inhabitants for the industry.

• Journal of the Ergonomics Society of Korea
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• v.30 no.5
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• pp.605-611
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• 2011

Objective: The aim of this study was to investigate the influence of seat heights for optimizing the ingress/egress performance in the elderly people. Background: Recently elderly users have been increasing in number and the ease of ingress/egress of the vehicle becomes an important issue. Method: Seven elderly subjects participated in this study(age: 71.7${\pm}$3.6yr, height: 167.7${\pm}$5.4cm, weight: 68.1${\pm}$11.5kg). Each subject performed the sitting and rising task from comfortable seated position on the chair under the following conditions: (1) with a lumbar support and (2) without a lumbar support. We measured EMG activities of seven muscles(tibialis anterior, soleus, gastrocnemius, vastus lateralis, rectus femoris, biceps femoris and erector supinae) and ranges of motion in lower limb during sitting and standing from three different heights (400mm, 500mm and 600mm from ground) of slide-up seat. Results: Muscle activities and angular movements of hip and knee joints during standing-up and sitting-down with a high seat height(600mm) were reduced mean 30.4% in extensor muscles, 57.11% in flexor muscle, 18.74% in erector muscle and 31.0% in joints compared with a low seat height(400mm). Conclusion: Muscle activities and joint movements in hip and knee were reduced when rising/sitting from a high seat height(600mm) compared with a low seat height(400mm). Application: This study can be used to design vehicle that are easy to get in and out of by older peoples with or without impairments.

• Spatial Information Research
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• v.20 no.3
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• pp.57-66
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• 2012

Gangwon-do has been suffering extensive landslide dam age, because its geography consists mainly of mountains. Analyzing the related factors is crucial for landslide prediction. We digitized the landslide and non-landslide spots on an aerial photo obtained right after a disaster in Inje, Gangwon-do. Three landslide factors-topographic, forest type, and soil factors-w ere statistically analyzed through GIS overlap analysis between topographic map, forest type map, and soil map. The analysis showed that landslides occurred mainly between the inclination of $20^{\circ}$ and $35^{\circ}$, and needleleaf tree area is more vulnerable to a landslide. About soil properties, an area with shallow effective soil depth and parent material of acidic rock has a greater chance of landslide.

• Journal of the Korean earth science society
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• v.24 no.4
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• pp.281-289
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• 2003

The purpose of this study is to isolate characteristics of exhibits particularly considering educational perspectives and effectiveness in attracting the attention of visitors in famous Natural History Museums. The target exhibits were selected from the Natural History museum in London, the Australian Museum in Sydney, the American Museum of Natural History in New York, and the Smithsonian Museum in Washington D.C. A total of 107 sample exhibits used in this study from these four museums were selected as representative solid earth science education related exhibitions including fossils, geological history, and minerals. For the investigation, this study developed a checklist with seven items based on reviewing previous literature and articles pertaining to exhibit characteristics of natural history museums and science centers. Also the educational perspectives focused on classifications of exhibitions by Hein (1997). In this study, all the sample exhibit, were taken pictures of and stored on slide films for lated examination. Respectively exciting and positive results were found in all four museums. The analyses, however, indicated weaknesses as well as strengths in the four museum exhibits.

• Journal of Dental Rehabilitation and Applied Science
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• v.31 no.3
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• pp.273-282
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• 2015

Centric occlusion-maximum intercuspation (CO-MI) discrepancy is one of main causes of evoking premature contact and resultant mandibular shift. These non-physiological conditions can induce temporomandibular disease, periodontitis, and non-carious cervical lesion. Therefore, if CO-MI discrepancy exists in patients who need extensive prosthetic rehabilitation, it must be corrected and then physiological occlusion must be restored. This report describes the treatment procedure of removing CO-MI discrepancy and prosthetic rehabilitation in a patient with 3.5 mm discrepancy, multiple caries and periodontitis. Proper mandibular position and modified opening & closing movement were confirmed by ARCUSdigma II and transcranial radiograph.