• Title/Summary/Keyword: slaughter method

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Rapid diagnosis of bovine tuberculosis in slaughter cattle using PCR (PCR 기법을 이용한 도축 소의 결핵병 신속진단)

  • Koh, Ba-Ra-Da;Kim, Hyun-Joong;Park, Duk-Woong;Park, Seong-Do;Kim, Jae-Ik;Park, Jong-Tae;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
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    • v.30 no.3
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    • pp.393-406
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    • 2007
  • Bovine tuberculosis is an important zoonosis worldwide. Mycobacterium bovis, the causative agent of this disease in cattle, is also a pathogen for humans and several economically important animals. The cases of tuberculosis are reported in two cow found at slaughter house located in Gwangju city. Histopathologically, in the lymph nodes, granulomas consisted of large areas of necrosis surrounded by variable thick bands of cellular infiltrate containing macrophages, Langhans-type multinucleated giant cells and lymphocytes. Lesions in the lung followed the same developmental pattern as did lesions in the lymph nodes with some exceptions. With the acid-fast staining, numerous mycobacteria were revealed in the lung and lymph nodes. M bovis was confirmed as a causative agent in these cattle using bacterial isolation and PCR and restriction fragment length polymorphism method based on a unique 12.7 kb fragment insertion sequence from the Mycobacterium tuberculosis genome and the pncA polymorphism, The insertion element IS6110 and IS1081 were present M bovis isolated from lungs and lymph nodes of cattle using PCR assay. These cases are interesting and important in public health aspect that M bovis-infected cattle were found during a routine post-mortem inspection at slaughter house.

Discrimination of cows from heifers by antemortem and postmortem inspection in slaughter house (도축장의 생.해체검사를 통한 경산우 및 미경산우 감별법(I))

  • Choi, Im-Yong;Choi, Tae-Suk;Lee, Dal-Ju;Lee, Hae-Yeong;Rho, Chang-Sik;Kim, Kyu-Hiun;Kim, Eun;Kweon, Taek-Boo;Lee, Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.30 no.3
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    • pp.429-436
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    • 2007
  • This study was performed to make helpful standard for discrimination of cows from heifers by uterine and ovarian section inspection. A total of 3,736 heifers and 249 cows were inspected from January to November 2006 in Karak Market Slaughter house. Firstly, it was obvious that non-corpus albicans were observed in almost heifers(3,597/3,736, 96.3%), but there were distinctive corpus albicans in all cows (249/249 100%). Secondly, another evident was that the length and diameter of the horn in uterus was below 30mm in 94.5% (3,267/3,736) heifers, but above 30mm in 90.4% (225/245) cows, and it was easy to distinguish between right and left horn by difference of size in 87.6% (218/249) cows. Thirdly the diameters of pelvic cavity was below 220mm in 96.8% (3,615/3,736) heifers, but was over 200mm in 85.1% (212/249) cows. Fourthly another difference was found in second sacrum union condition and 13th thoracic vertebral process's states. In case of heifers, it was distinctive five lines in each second sacrum, but it was non-distinctive or united in the same each lines of the sacrum in cows. Fifthly in the results of 13th thoracic vertebral colors, the ratio between red color and white color was 7 : 3 in heifers (3,385/3,736, 90.6%), but that was 2 : 8 in cows (199/249, 79.9%). Consequently, it was possible to use the method which discriminated cows from heifers by uterine and ovarian section inspection in large scaled field slaughter house.

A STANDARD METHOD FOR JOINTING CAMEL CARCASSES WITH REFERENCE TO THE EFFECT OF SLAUGHTER AGE ON CARCASS CHARACTERISTICS IN NAJDI CAMELS. 3. PARTITION AND DISTRIBUTION OF CARCASS FAT

  • Abouheif, M.A.;Basmaeil, S.M.;Bakkar, M.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.4 no.3
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    • pp.219-225
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    • 1991
  • The influence of age on the relative growth patterns of subcutaneous, intermuscular, intramuscular, perirenal, channel and hump fat in relation to the total fat weight in carcass sides of 18 Najdi male camels averaging 8, 16 and 26 months of age has been investigated. The total fat weight in a carcass side increased (p<.01) from 17.3% to 27.1% as the camel age increased from 8 to 26 months. However, at all ages studied, intermuscular fat weight was the largest fat depot, followed, in order, by subcutaneous and intramuscular fat. The change in weight of the intramuscular, intermuscular and subcutaneous fat between 8 and 26 months of age was greater, reaching 6.7, 4.3 and 4 times respectively, than the hump, channel and perirenal fat weight which increased by 3.6, 2.5 and 2.3 times, respectively. The allometric growth coefficient (${\beta}$) for intramuscular fat in relation to the total carcass fat weight was the highest, followed, in order, by intermuscular, subcutaneous, hump, channel and perirenal fat.

A STANDARD METHOD FOR JOINTING CAMEL CARCASSES WITH REFERENCE TO THE EFFECT OF SLAUGHTER AGE ON CARCASS CHARACTERISTICS IN NAJDI CAMELS. II. VARIATION IN LEAN GROWTH AND DISTRIBUTION

  • Abouheif, M.A.;Basmaeil, S.M.;Bakkar, M.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.3
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    • pp.155-159
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    • 1990
  • The growth of lean from nine wholesale cuts in relation to the total lean weight in carcass side were evaluated with three allometric equations for 18 Najdi male camels slaughtered at 8, 16 and 26 months of age. The allometric growth coefficients indicated that as the camels grew, weights of lean form brisket and flank cuts increased relatively more rapidly than the total lean in carcass side (${\beta}$ >1.1) and that weights of lean from wholesale shoulder and rump cuts increased relatively less rapidly (${\beta}$ <.9) than the total lean weight from carcass side. The growth coefficients for the lean in the thoracic limb and pelvic limb showed a dorsoventral rise in the growth impetus, with coefficients for the thoracic limb correspondingly higher than those of pelvic limb. The growth coefficients for the lean from shoulder, rib, flank and leg cuts increased (p<.01) with increased weight of total lean in carcass side, whereas it decreased with increased total lean weight in carcass side for the lean in neck, brisket, plate, loin and rump cuts. At a constant weight of total lean in carcass side, older camels had larger coefficients for lean in neck, brisket, rump, flank and leg cuts, but had smaller coefficients for lean in shoulder, rib, plate and loin cuts.

Comparison of Blood Loss and Meat Quality Characteristics in Korean Black Goat Subjected to Head-Only Electrical Stunning or without Stunning

  • Bakhsh, Allah;Ismail, Ishamri;Hwang, Young-Hwa;Lee, Jung-Gyu;Joo, Seon-Tea
    • Food Science of Animal Resources
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    • v.38 no.6
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    • pp.1286-1293
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    • 2018
  • This study assessed the effects of non-stunning (NS) and head-only electrical stunning (HOES) slaughtering condition on meat quality traits of longissimus lumborum (LL) muscle from Korean black goat (KBG). Ten KBGs (18 months) were assigned into two groups and exposed to either NS or HOES treatments. Blood loss (BL) % was measured after exsanguination, and meat quality traits including muscle pH, meat color measurements (CIE $L^*$, $a^*$, $b^*$, Chroma, and hue angle), water-holding capacity (WHC), Warner-Bratzler shear force (WBSF), and sarcomere length were measured at 24 h postmortem. Results indicated that NS and HOES had no significant difference on BL %, the rate of pH decline, meat color properties, and WHC (p>0.05). It has only a small effect on WBSF and sarcomere length values, but the difference was marginal. These results suggested that meat quality of LL muscle from goat might not be affected by slaughter methods because neither NS nor HOES did result in poor quality of meat.

A Preliminary Study: Antibiotic Resistance of Escherichia coli and Staphylococcus aureus from the Meat and Feces of Various South African Wildlife Species

  • van den Honert, Michaela Sannettha;Gouws, Pieter Andries;Hoffman, Louwrens Christiaan
    • Food Science of Animal Resources
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    • v.41 no.1
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    • pp.135-144
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    • 2021
  • This study determined the antibiotic resistance patterns of Escherichia coli and Staphylococcus aureus from the raw meat and feces of three game species from three different farms across South Africa. The Kirby-Bauer disk diffusion method was used according to the Clinical and Laboratory Standards Institute 2018 guidelines. E. coli was tested against ampicillin, ceftazidime, chloramphenicol, streptomycin, sulphafurazole and tetracycline. S. aureus was tested against tetracycline, erthromycin, vancomycin, penicillin, oxacillin and cefoxitin. There were no significant differences in the E. coli antibiotic resistance profiles between the meat and fecal samples (except towards ceftazidime where 5% of the meat isolates were resistant and 0% of the fecal isolates). The S. aureus meat isolates showed high (75%) resistance towards penicillin and on average, 13% were resistant to oxacillin/ cefoxitin, indicating methicillin resistance. The results from this study indicate that there is incidence of antibiotic resistant bacteria from the feces and meat of wildlife species across South Africa, suggesting that cross contamination of the meat occurred during slaughter by antibiotic resistant bacteria from the abattoir personnel or equipment and or from carcass fecal matter. In addition, the results highlight the importance of food safety and hygiene procedures during slaughter to prevent cross-contamination of antibiotic resistant bacteria, as well as pathogens, onto raw meat.

Modeling net energy requirements of 2 to 3-week-old Cherry Valley ducks

  • Yang, Ting;Yu, Lexiao;Wen, Min;Zhao, Hua;Chen, Xiaoling;Liu, Guangmang;Tian, Gang;Cai, Jingyi;Jia, Gang
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.10
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    • pp.1624-1632
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    • 2020
  • Objective: A total of three hundred unsexed ducks were utilized to estimate net energy requirements of maintenance (NEm) and weight gain (NEg) for 2 to 3-week-old Cherry Valley ducks and to establish a model equation to predict NE requirements using the factorial method. Methods: To determine the apparent metabolizable energy (AME) of the diet, fifty 7-day-old ducks at approximately equal body weights (BWs) were randomly assigned into five groups that were fed at different levels (ad libitum, 85%, 75%, 65%, and 55% of ad libitum intake), and the endogenous acid-insoluble ash as indigestible marker. The two hundred and fifty 7-day-old ducks were used for a comparative slaughter experiment. At the beginning of the experiment, ten ducks were sacrificed to determine the initial body composition and energy content. The remaining ducks were randomly assigned into five groups (same as metabolic experiment). Ducks of the ad libitum group were slaughtered at 14 and 21-day-old. At the end of the experiment, two ducks were selected from each replicate and slaughtered to determine the body composition and energy content. Results: The results of the metabolizable experiment showed AME values of 13.43 to 13.77 MJ/kg for ducks at different feed intakes. The results of the comparative slaughter experiment showed the NEm value for 2 to 3-week-old Cherry Valley ducks was 549.54 kJ/kg of BW0.75/d, and the NEg value was 10.41 kJ/g. The deposition efficiency values of fat (Kf) and crude protein (Kp) were 0.96 and 0.60, respectively, and the values of efficiency of energy utilization (Kg) and maintenance efficiency (Km) were 0.75 and 0.88, respectively. Conclusion: The equation for the prediction of NE requirements for 2 to 3-week-old Cherry Valley ducks was the following: NE = 549.54 BW0.75+10.41 ΔW, where ΔW is the weight gain (g).

A Study on the Bovine Sarcocystis -(Report II ):A Survey on the Bovine Sarcocystis in the Muscles of Heart, Liver, Kidney, Tongue, Diaphragm and Masseter - (소의 주근포자충에 관한 연구 -(제 II 보) : 심장, 간장, 신장, 설, 횡격막 및 교근내 주근포자충의 조사연구-)

  • Yoon Hwa-Joong;Ra Seong-An
    • Journal of the korean veterinary medical association
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    • v.24 no.2
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    • pp.92-97
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    • 1988
  • One hundred eighteen cattle in the slaughter houses of kyeong ki-do were examined for Sarcocystis cruzi using macroscopic and microscopic method of detection. Sausage-shaped Sarcocystis were detected from the heart, diaphragm, tongue, masseter, liver, kid

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Comparison of residual antibiotic materials in meet -Slaughtered cattle and swine in Seoul- (식육중 잔류항균물질 비교 조사 -서울지역 도축 소와 돼지를 중심으로-)

  • 변정옥;강영일;이달주;황래홍;이양수;이병동
    • Korean Journal of Veterinary Service
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    • v.25 no.3
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    • pp.229-236
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    • 2002
  • This study was carried out to compare the residual antibiotic materials in muscles of slaughter cattle and swine from slaughterhouses in Seoul from 2000 to 2001 by EEC-4-plate method, Charm II and HPLC method. 1. Residual antibiotic materials were detected from 95 samples(0.8%) by EEC-4-plate and 57 samples(10.2%) by Charm II. The final HPLC method determined the positives are 43(45.3%) and 27(47.3%) respectively. 2. The detection ratios were 45% by EEC-4-plate and 47% by Charm II. 3. Seventy samples were classified as tetracyclines 56(75.7.4%), sulfonamides 10(14.9%), $\beta$-lactam 6(8.1%) chloramphenicol 1(1.4%). Three of them were confirmed to be positive simmultaneously for tetracyclines, sulfonamides and chloramphenicol. 4. The highest residual concentration of chlortetracycline, oxytetracycline, sulfamethazine, sulfadimethoxine, sulfaquinoxaline, penicillin, ampicillin and chloramphenicol were 0.34, 11.29, 68.16, 0.13, 4.0, 0.12, 0.4 and 0.04ppm, respectively.

Evaluation of Amino Acid and Energy Utilization in Feedstuff for Swine and Poultry Diets

  • Kong, C.;Adeola, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.7
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    • pp.917-925
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    • 2014
  • An accurate feed formulation is essential for optimizing feed efficiency and minimizing feed cost for swine and poultry production. Because energy and amino acid (AA) account for the major cost of swine and poultry diets, a precise determination of the availability of energy and AA in feedstuffs is essential for accurate diet formulations. Therefore, the methodology for determining the availability of energy and AA should be carefully selected. The total collection and index methods are 2 major procedures for estimating the availability of energy and AA in feedstuffs for swine and poultry diets. The total collection method is based on the laborious production of quantitative records of feed intake and output, whereas the index method can avoid the laborious work, but greatly relies on accurate chemical analysis of index compound. The direct method, in which the test feedstuff in a diet is the sole source of the component of interest, is widely used to determine the digestibility of nutritional components in feedstuffs. In some cases, however, it may be necessary to formulate a basal diet and a test diet in which a portion of the basal diet is replaced by the feed ingredient to be tested because of poor palatability and low level of the interested component in the test ingredients. For the digestibility of AA, due to the confounding effect on AA composition of protein in feces by microorganisms in the hind gut, ileal digestibility rather than fecal digestibility has been preferred as the reliable method for estimating AA digestibility. Depending on the contribution of ileal endogenous AA losses in the ileal digestibility calculation, ileal digestibility estimates can be expressed as apparent, standardized, and true ileal digestibility, and are usually determined using the ileal cannulation method for pigs and the slaughter method for poultry. Among these digestibility estimates, the standardized ileal AA digestibility that corrects apparent ileal digestibility for basal endogenous AA losses, provides appropriate information for the formulation of swine and poultry diets. The total quantity of energy in feedstuffs can be partitioned into different components including gross energy (GE), digestible energy (DE), metabolizable energy (ME), and net energy based on the consideration of sequential energy losses during digestion and metabolism from GE in feeds. For swine, the total collection method is suggested for determining DE and ME in feedstuffs whereas for poultry the classical ME assay and the precision-fed method are applicable. Further investigation for the utilization of ME may be conducted by measuring either heat production or energy retention using indirect calorimetry or comparative slaughter method, respectively. This review provides information on the methodology used to determine accurate estimates of AA and energy availability for formulating swine and poultry diets.