• Title/Summary/Keyword: skin layer

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Development of Rodent Repellent Cable With High Stability (고안정성을 가진 방서 케이블의 개발)

  • Yang, W.Y.;Kang, T.O.;Kim, K.S.;Chun, C.O.
    • Proceedings of the KIEE Conference
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    • 1994.07b
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    • pp.1354-1356
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    • 1994
  • In order to protect cable from rat's biting, a development of rodent repellent is described. This cable has double coated sheath such as construction of skin layer over the conventional cable sheath. Stabilized rodent repellent agent which has good compatibility with PVC is employed in a skin layer. Rodent repellent layer has highly residual properties of agent through the manufacturing process, and meets the general requirements of cable sheath. Also, rat repellent effect is excellent.

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Early Healing Responses of the Skin Wounds in the Korean Fire-Bellied Toad, Bombina orientalis (무당개구리 (Bombina orientalis) 피부의 초기 상처치유반응)

  • Jeong, Moon-Jin;Moon, Myung-Jin
    • Applied Microscopy
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    • v.28 no.3
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    • pp.329-343
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    • 1998
  • Early wound healing responses in the skin of the Korean fire bellied toad, Bombina orientalis, were examined using histochemical , scanning and transmission electron microscopical techniques. Cutaneous wounds were induced by sharp razor blade , and maintained in special cages for up to 3 days. By this treatment whole parts of epithelium and dermis were profoundly affected. After injury, retraction of damaged surface of the skin was observed. At 3 hours after injury, poly-band was form by mucous substance. The wound skin consisted of mucous gland secreted mucous substance which flows into wound cavity and the wound area was sealed by mucous substance which form thick layer at 6 hours. It appeared that natural disposition showed acid and neutral mucous substance by reaction of PAS and Alcian blue (pH 2.5) staining. After formation of the poly-band, epithelial cells were formed by migration of regenerating epithelial cells. These cells moved over the wound surface by migration at 12 hours after wounding. Poly-band was much increased in thickness and migrating cells were completely formed wound closure at 24 hours post-wounding. After 72 hours regenerated epithelial layer consists of four or five cell layers which are similar to clear, granular, and spinous layer.

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Structure and Histochemistry of Skin of Mud Loach, Misgurnus anguillicaudatus (Pisces, Cobitidae), from Korea (한국산 미꾸리 Misgumus anguillicaudatus (Pisces, Cobitidae) 피부 구조 및 조직화학적 특징)

  • Park, Jong-Young;Kim, Ik-Soo
    • Korean Journal of Ichthyology
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    • v.11 no.2
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    • pp.109-116
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    • 1999
  • The structure of skin of a mud loach Misgurnus anguillicaudatus was described in relation with their histochemical nature from four regions of the skin. The epidermis has a strongly thick layer of two glandular cells, consisting of a elongate mucous cell and club cell, and a thin layer of superficial layer. The secretion of the elongated mucous cell was acid mucopolysaccharides in nature but the club cell did not give any histochemical reaction. A well defined lymphatic system, comprising small lymphocytes was present in the stratum germinativum layer of the epidermis. A pit organ of a pear-shaped structure was present below the epithelial cells and lie directly on the basement membrane. The organ has blood vessels serving the sense organs of the epidermis. There was a definite area showing acid mucopolysaccharides in the stratum laxum layer of the dermis. Small scales are present deep in the dermis except the top of the head. A great number of blood capillaries were found just under basement membrane. These structural features of skin in M. anguillicaudatus seem to be closely related with cutaneous respiration using air.

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Radioprotective effects of dimethyl sulfoxide in the artificial skin reconstructed with cultured human cells (배양된 사람 세포를 이용해 제작한 인공피부에서 방사선조사에 대한 dimethyl sulfoxide의 보호 효과)

  • Ryu Young-Ha;Choi Karp-Shik;Song In-Hwan
    • Imaging Science in Dentistry
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    • v.32 no.1
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    • pp.11-18
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    • 2002
  • Purpose: To evaluate cultured human artificial skin as an experimental model for studying radiation effects in vitro. Materials and Methods: The skin was constructed by culturing keratinocytes over collagen lattice which made by culturing fibroblasts. Two groups were irradiated to gamma rays at single dose of 25 Gy with or without 3.5% of DMSO. Ultrastructures were investigated by electron microscopy after irradiation. The number of epidermal layers and expression of cytokeratin (CK) 14 & 10 were also seem by light microscopy. Results: At 2 days after irradiation in experimental group without DMSO, necrotic cells were rarely found in the spinosal layer and undercornified cells were visible in the homey layer. Similar findings were also found in experimental group with DMSO but in mild form. The number of epidermal layers in experimental group without DMSO were significantly fewer than other group. CK 14 expressed in all the layer excluding homey layer but CK 10 expressed over 3∼4 basal layers. Such patterns of CK expression were similar to all groups. It is suggested that structures of the keratinocytes and epidermal formation could be disturbed by irradiation in artificial skin and that DMSO can protect these damages. Conclusion : Therefore this work could be used as an organotypic experimental model in vitro using human cells for studying radiation effect in skin. Furthermore structural findings provided in this study could be used as useful basic data in further study using this model.

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Correlation of the Wall Skin-Friction and Streamwise Velocity Fluctuations in a Turbulent Boundary Layer(II) (난류경계층에서 벽마찰력과 유동방향 속도성분과의 상관관계(II))

  • Yang, Jun-Mo;Yu, Jeong-Yeol;Choe, Hae-Cheon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.21 no.3
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    • pp.427-435
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    • 1997
  • Conditional sampling techniques are utilized to investigate the relation between the wall skin-friction and stream wise velocity fluctuations in a turbulent boundary layer. Conditionally averaged results using a peak detection and the VITA (variable-interval time-averaging) technique show that a high skin friction is associated with high frequency components of the wall skin-friction fluctuations. The conditionally averaged wall skin-friction fluctuations obtained by using the VITA technique have a positively-skewed characteristics compared with the conditionally averaged stream wise velocity fluctuations. It is confirmed that there exists a phase shift between the wall skin-friction and stream wise velocity fluctuations, which was also found from the long-time averaged space-time correlations. The amount of phase shift between the wall skin-friction and stream wise velocity fluctuations is the same as that from the long-time averaged space-time correlations and does not change despite the variation of the detection threshold.

Three-Dimensional Skin Tissue Printing with Human Skin Cell Lines and Mouse Skin-Derived Epidermal and Dermal Cells

  • Jin, Soojung;Oh, You Na;Son, Yu Ri;Kwon, Boguen;Park, Jung-ha;Gang, Min jeong;Kim, Byung Woo;Kwon, Hyun Ju
    • Journal of Microbiology and Biotechnology
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    • v.32 no.2
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    • pp.238-247
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    • 2022
  • Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.

Quality Characteristics of Yellow Layer Cake Added with Coffee Silver Skin (커피 실버스킨을 첨가한 옐로우 레이어 케이크의 품질특성)

  • An, Hye-Lyung;Hwang, Yoon-Kyung
    • Culinary science and hospitality research
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    • v.19 no.3
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    • pp.33-45
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    • 2013
  • This study was conducted to evaluate the effect of substitution of the flour with coffee silver skin (at the levels of 0%, 3%, 5%, and 7%) on the characteristics of yellow layer cake. The specific gravity of batter increased significantly as the content of silver skin increased. The specific volume of cake decreased significantly as the content of silver skin increased. The volume index decreased significantly as the silver skin content increased, while the symmetry index and uniformity index were not influenced. It was found that control had the highest cake indexes. Texture analysis showed that control had the lowest hardness and highest springiness. Hardness value of cakes was increased by adding silver skin; however, the springiness value of cakes with silver skin decreased during storage. According to sensory evaluation, experiment groups scored the higher points in texture acceptance than control. Above all, SS5 scored the highest points in aroma, taste and SS3 scored the highest points in appearance and overall acceptance.

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Experimental Investigation on the Drag Reduction Mechanism of Outer-layer Vertical Blades Array using Stereoscopic Time-Resolved PIV (스테레오 시간분해 입자영상유속계를 이용한 외부경계층 수직날 배열에 의한 마찰저항 저감 기구에 관한 실험적 조사)

  • Lee, Inwon;Park, Seong-Hyeon;Chun, Ho-Hwan;Hwang, Arom;An, Nam-Hyun
    • Journal of Power System Engineering
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    • v.17 no.6
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    • pp.95-101
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    • 2013
  • A stereo PIV measurements in a circulating water channel has been performed to investigate the skin friction reduction mechanism of the outer-layer vertical blades first devised by Hutchins. In a recent PIV measurement study, considerable skin friction reduction was achieved as much as 2.73%~7.95% by outer-layer vertical blades array. In the present study, the influence of vertical blades array upon the characteristics of the turbulent coherent structures was analyzed by proper orthogonal decomposition method. It is observed that the vortical structures are cut and deformed by blades array and also the turbulent intensity and the Reynolds stress were weakened by the blades. These phenomena strongly associate the skin-friction drag reduction mechanism in the turbulent boundary layer flow.

Microstructural Morphology and Bending Performance Evaluation of Molded Microcomposites of Thermotropic LCP and PA6 (액정폴리머/폴리아미드6 미시복합재료의 내부구조 및 기계적 굽힘성능 평가)

  • ;Kiyoshi Takahashi
    • Composites Research
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    • v.12 no.6
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    • pp.53-64
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    • 1999
  • Microstructural morphology and bending strengths of moulded composites of thermotropic liquid crystalline polymer(LCP) and polyamide 6 (PA6) have been studied as a function of epoxy fraction. Injection-moulding of a composite plaque at a temperature below the melting point of the LCP fibrils generated a multi-layered structure: the surface skin layer with thickness of $65\;-\;120{\mu\textrm{m}}$ exhibiting a transverse orientation; the sub-skin layer with an orientation in the flow direction; the core layer with arc-curved flow patterns. The plaques containing epoxy 4.8vol% exhibited superior bending strength and large fracture strain. With an increase of epoxy fraction equal to and beyond 4.8vol%, geometry of LCP domains was changed from fibrillar shape to lamella-like one, which caused a shear-mode fracture. An analysis of the bending strength of the composite plaques by using a symmetric layered model beam suggested that addition of epoxy component altered not only the microstructural geometry but also the elastic moduli and strengths of the respective layers.

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Characteristics of Strength and Deformation of Aluminum Honeycomb Sandwich Composites Under Bending Loading (굽힘 하중을 받는 알루미늄 하니컴 샌드위치 복합재료의 강도 및 변형 특성)

  • Kim Hyoung-Gu;Choi Nak-Sam
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2004.10a
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    • pp.61-64
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    • 2004
  • The strength characteristics as well as deformation behaviors of honeycomb sandwich composite (HSC) structures were investigated under bending in consideration of various failure modes such as skin layer yielding, interface-delamination, core shear deformation and local buckling. Deformation behaviors of honeycomb sandwich plates were observed with various types of aluminum honeycomb core and skin layer. Their finite-element analysis simulation with a real model of honeycomb core was performed to analyze stresses and deformation behaviors of honeycomb sandwich plates. Its results were very comparable to the experimental ones. Consequently, the increase in skin layer thickness and in cell size of honeycomb core had dominant effects on the strength and deformation behaviors of honeycomb sandwich composites.

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