• 한국패션뷰티학회지
• /
• 제3권3호
• /
• pp.31-40
• /
• 2005

The reason that aromatherapy began to draw attention again as the alternative therapy is because the essential oils that are used for aromatherapy not only allow the human body to maintain homeostasis but also have an effect on curing a mental feeling of fatigue and because these efficacies and effects are proceeding with being proved scientifically. Also, with coming to be high in a voice of concern about severally side effects that the modern medicine and the synthetic chemistry have, the aromatherapy came to be paid attention as the replacement therapy that can substitute for and supplement this. In particular, at the time of requiring a study on a substance to substitute for this after having been announced in Japan for 1998 a risk of Zincpyrithione that is being used as a component of dandruff treatment, it is thought that the aroma oil that was known for having an anti-dandruff effect has a merit of being easy in a user's access, thus it will be effective in curing the symptom of dandruff. Accordingly, this study conducted the following experiment in a bid to examine whether the aroma oil has the anti-microbial effect on the actual malassezia. First of all, it measured the minimum concentration of aroma oil in which the growth of malassezia is inhibited, by carrying out MIC(Minimum Inhibition Concentration) test in terms of anti-microbial activity against Malassezia furfur KCTC 7744 while using oils of Patchouli, Rosemary, Lavender, and Tea tree among aroma oils that are being used for dandruff treatment. As a result of that, Tea tree oil has the most excellent anti-microbial activity, and the measurement result of anti-microbial activity was shown in order of Lavender oil>Rosemary oil>Patchouli oil. Next, it carried out the is actually eased by applying Tea tree oil directly to the scalp of dandruff. In consequence of the experiment, the massage of Tea tree oil indicated the result of relieving the symptoms of dandruff such as scale, itch, pain, inflammation, and sebum, and among these symptoms of dandruff, it was shown to be most effective in easing itch. Especially, this clinical experiment was indicated that the massage of Tea tree oil has the most effect on relieving itch of targets with a dry-skin type who had no experience of dandruff treatment.

• 대한화장품학회지
• /
• 제46권3호
• /
• pp.253-263
• /
• 2020

활성산소종(reactive oxygen species, ROS)은 우리 몸의 항상성 유지에 있어 중요한 역할을 한다. 그러나 과도한 ROS의 생성은 단백질, 지질, 핵산과 같은 세포 구성성분을 손상시키고 피부노화를 촉진시킨다. 이에 본 연구에서는 과도한 산화 스트레스를 예방하기 위해 Chungsimyeonja-um (CSYJE)의 항산화 효과를 확인하였다. 먼저 DPPH 및 ABTS assay를 실시하여 CSYJE의 항산화 효과를 확인한 결과 농도 의존적으로 radical 소거 활성을 확인하였다. 세포생존율 확인을 위해 MTT assay를 실시한 결과 1,000 ㎍/mL 농도에서 세포 독성이 없음을 확인하였다. 항산화 관련 단백질인 nuclear-E2-related factor 2 (Nrf2), Heme oxygenase-1 (HO-1)의 발현 수준을 확인하기 위해 western blotting을 실시한 결과 농도 의존적으로 발현이 증가하는 것을 확인하였다. 세포 내 ROS유발 물질인 lipopolysaccharide (LPS)로 ROS를 유도한 후, ROS생성 억제효과를 확인하기 위해 DCF-DA 염색법을 실시한 결과 농도 의존적으로 ROS 생성 억제효과를 확인하였으며 ROS의 생성으로 인한 염증성 사이토카인과 염증인자의 mRNA발현 수준을 확인하기 위해 real-time RT-PCR을 실시한 결과 농도 의존적으로 염증성 사이토카인과 염증인자의 mRNA 발현을 억제시켰다. 따라서, 본 연구는 Nrf2/HO-1 신호 전달 경로 활성을 통해 CSYJE의 항산화효과를 확인했으며 이는 CSYJE가 활성산소를 억제하여 항산화 화장품의 재료로서 사용될 수 있음을 시사한다.

• 한국지역사회생활과학회지
• /
• 제24권4호
• /
• pp.543-552
• /
• 2013

Physiological and subjective responses of the farmers and thermal environment during chili harvest in the open field were investigated to evaluate the thermal environments and farmers's workload. Eight career female farmers in their sixties participated as subjects both in morning work(MW, AM 9:00~10:30) and in afternoon work(AW, PM 15:00~16:30) with each lasting about 90 minutes. The results were as follows. 1) Air temperature, air humidity, globe temperature and WBGT of MW were mean $25.54^{\circ}C$, 81.82%RH, $37.72^{\circ}C$, $26.27^{\circ}C$ and AW were mean $30.63^{\circ}C$ 82.50%RH, $40.11^{\circ}C$, $30.02^{\circ}C$, respectively. By the WBGT, we evaluated that the thermal environment in the afternoon in the open field gave a thermal burden to farmers. 2) Mean skin temperature of AW($34.8{\pm}0.8^{\circ}C$) was higher than MW($33.5{\pm}1.2^{\circ}C$)(p<0.05). Clothing microclimate temperature on the chest of each work time were $31.3^{\circ}C$(MW) and $32.7^{\circ}C$(AW). Clothing microclimate humidity on the chest of each work time were over 80%RH. Heart rate were 88.5bpm(MW) and 91.7bpm(AW) respectively. 3) Farmers working in the afternoon felt uncomfortable after 45~60 min. of work and in the morning they felt uncomfortable after 90 min. of work. We evaluated that the harvesting of chilies in the open field was 'moderate work' by the physiological responses but the level of thermal burden increased over time especially in the afternoon work. It is suggested that farm workers should drink fluids between work to stay in homeostasis by sweating and to take frequent rests. Active clothing ventilation and wearing functional garments would help farm workers excrete sweat effectively.

• 생명과학회지
• /
• 제32권5호
• /
• pp.391-410
• /
• 2022

인간 미생물 균총은 장, 구강, 피부와 같이 체내외 다양한 부위에 존재하는 박테리아, 균류, 바이러스 등을 포함하는 미생물 집단이다. 16s ribosomal RNA에 대한 대사체 분석 및 차세대 염기서열 분석기술의 개발과 함께, 살아있는 유기체 내에 존재하는 미생물 균총에 대한 많은 연구가 진행되었다. 이에 따라, 미생물 균총이 숙주의 대사 및 면역과정과 복잡하게 연관되어 있음이 확인되었다. 공생균(commensal microbiota)이라 불리는 미생물 균총의 특정 박테리아가 필수 영양소를 생성하거나 다른 병원성 미생물로부터 숙주를 보호하여 긍정적으로 영향을 미치고 있는 반면, 비정상적인 미생물 균총의 조성을 의미하는 미생물 균총의 불균형(dysbiosis)에 의해 체내 항상성 유지를 방해하여 다양한 종류의 질병을 발생시키기도 한다. 최근, 미생물 균총 중에서도 구강과 장내 존재하는 박테리아가 위장관암의 발암과정과 항암제의 치료효과에 상당한 영향을 미치고 있음이 여러 논문을 통해 보고되고 있다. 미생물 균총-암-면역계 사이의 복잡한 연관성과 미생물 균총 기반 발암 메커니즘에 대한 규명은 암에 대한 이해와 새로운 항암제 개발에 중요한 단서를 제공할 것으로 기대된다. 본 리뷰는 미생물 균총의 박테리아가 위장관암과 항암제에 어떤 영향을 미치고 있는지에 대해 초점을 맞추고 있는 논문들을 요약하고 있으며, 나아가 기존 항암제의 치료효과를 개선하기 위해 복합제로써 미생물 균총의 잠재력과 도전과제에 대해 논의한다.

• 핵의학기술
• /
• 제19권2호
• /
• pp.112-114
• /
• 2015

Purpose Vitamin D to Anti- Rickets both steroid compounds showing activity, By acting on bone tissue secretary and the key to maintain serum Ca homeostasis. The blood level of vitamin D is the largest in D3 that the concentration of the metabolite is reflected in the holding state of vitamin D in vivo. Sunlight to change the 7-dehydrocholesterol in the skin and through the skin to D3, In the liver in combination with the D2 and D3 D4 changes. The Radioimmunoassay(RIA) method is measuring the D 3, the sensitivity can be measured also difficult trace substance to measure the normal test because it is very sensitive, but recently, a check is possible, for the Total D3 in Chemimicroparticle immunoassay(CMIA) or Chemiluminescent immunoassay(CLIA) measuring using microparticle RIA and CMIA(Architect i2000SR) / use the CLIA(DXI-800) method to compare and evaluate the correlation between the tests in the same test items. Materials and Methods Commissioned from January 2014 to March 2015 patients were enrolled in a total of 273 people. 29 out of 273 people conducted by RIA were compared with CMIA, 244 patients were compared with CLIA. Using reagents and equipment were used RIA(Diasource), CMIA(Architect i2000SR, Abbott Diagnostics) / CLIA( Unicel DXi-800, Beckman coulter). Results Correlation of the RIA and CLIA was a R2 = 0.1844 (y = 0.7303x + 3.9005), and the correlation of RIA CMIA is R2 = 0.2762 (y = 0.8862x + 4.56) respectively. (According to statistics, during the same period RIA is Deficiency 4.31%, Insufficiency 90.53%, Sufficiency 5.16%, was Excess 0%, CLIA / CMIA is Deficiency 17.02%, Insufficiency 75.91%, Sufficiency 7.03%, indicating the distribution of 0.03 % Excess) Conclusion Serum vitamin D and parathyroid hormone that show an inverse relationship, the level above which are not parathyroid hormone and vitamin D reduced the increase. The density is different for each study, at most 20 is reported to be the maximum between 30 ng / ml. In Korea it requires a proposed standard of vitamin D deficiency, reference to the WHO lack the case more than 10ng/ml, 20ng/ml and defined by the lack of, if not more than, the IOM, but looking at 12ng/ml or less to the normal to lack, at least 20ng/ml, the reference do not match the deficit under 20ng/ml, 21-29ng/ml relative lack between, was also defined as a sufficient condition for more than 30ng/ml. Although not statistically is between RIA and CLIA two ways to vitamin D levels change according to season match, when seasonally seen in summer as commonly known (April to September), winter (October to March) relative to the increase measured than it was found. Finally, the study on the correlation between the two methods have been expected to result in a consistent and apply the same view high reference value on the graph is difficult. However, there may be differences between the test equipment and methods, and could be especially the case of RIA method using an organic solvent is difficult to compare different methods and correlated view similar trend in vitamin D deficiency and quarterly aspect ratio.

• 생명과학회지
• /
• 제31권8호
• /
• pp.719-728
• /
• 2021

멜라닌 색소는 포유동물의 피부, 머리카락, 눈, 신경계에 풍부하게 존재한다. 멜라닌은 다양한 환경적 스트레스로부터 피부를 보호하며, 생리학적 산화-환원 완충 작용을 통해 항상성을 유지한다. 그러나, 과도한 멜라닌 축적은 간반, 주근깨, 노인성 흑자, 염증성 색소침착을 일으킬 수 있다. 티로시나아제는 멜라닌의 생합성 경로 조절에 아주 중요한 역할을 하는 것으로 알려져 있다. 티로시나아제의 활성을 저해하는 다양한 미백제가 개발되었지만 알러지, DNA손상, 세포독성, 돌연변이 유발 등을 야기하는 부작용으로 인해 임상적 적용이 제한되었다. 본 논문에서 여러 4-크로마논 유도체를 합성하여 티로시나아제 억제 활성을 조사하였다. 이들 화합물 중 MHY1294는 IC₅₀가 5.1±0.86 μM으로 양성 대조군인 코직산(14.3±1.43 μM) 보다 나은 티로시나아제 효소 억제 활성을 나타냈다. 또한 MHY1294는 티로시나아제의 촉매 부위에서 경쟁적인 억제 작용을 보였으며 코직산보다 더 큰 기질 결합 친화성을 가지는 것으로 확인되었다. 뿐만 아니라, MHY1294는 B16F10 흑색종 세포에서 멜라닌 세포 자극 호르몬 (α-MSH)에 의해 유도되는 멜라닌 합성과 세포 내 티로시나아제 활성을 유의적으로 억제하였다. 결론적으로 본 연구는 MHY1294가 과도한 멜라닌 축적에 대한 약물 제제 및 미백제로서의 개발 가능성이 있음을 시사한다.