• Journal of Forest and Environmental Science
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• 제20권1호
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• pp.28-39
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• 2004

본 연구는 강원도 내 자연휴양림의 프로그램의 현황을 파악하여 향후 자연휴양림의 발전 방향을 모색하고자 실시하였다. 2003년 현재 강원도는 19개소의 자연휴양림(국유림 11 개소, 공유림 4개소, 사유림 4개소)을 조성 운영하고 있으며, 현행 시행되고 있는 프로그램으로는 숲해설, 산악자전거, 산악 마라톤, 산악스키, 숲속 음악회, 숲교실 등이 시행되고 있는 것으로 나타났다. 본 연구에서는 강원도 자연휴양림 프로그램의 새로운 방향을 위해서 문화 복지 교육 프로그램을 축으로 하여 발전 방향을 모색하였다. 문화 프로그램으로는 "전통가옥체험 프로그램", "오지체험 프로그램", "산촌 특산물 프로그램"을 구상하였으며, 복지 프로그램은 "노인복지프로그램"과 "장애인 복지 프로그램"을, 교육프로그램으로는 "숲속의 지구촌 교류" 프로그램 조성과 "인성교육 프로그램"을 제시하였다. 앞으로 주 5일 근무제 도입으로 휴양수요가 대폭적으로 증가할 것으로 예상되므로 관광 강원도의 이미지를 부각시키기 위해서 자연휴양림의 지속적인 관심과 다양한 프로그램 개발이 더욱 증진되어야 할 것이다.

• 한국환경복원기술학회지
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• 제18권6호
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• pp.201-214
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• 2015

This study was conducted to identify forest topsoil's usefulness from July 2013 to Oct 2014. Generally, there are abundant seeds in topsoils and it can be used at restoration works. Mt. GARIWANG is famous for biodiversity in Korea. This study was aimed to find out the suitable utilization methods of natural topsoils for restoration works at sky-slopes construction sites. Test beds was made to identify suitable collecting times and suitable topsoil thickness. The main results are summarized as follows. First, Mt. GARIWANG have diverse species and in topsoils we can find lots of burried seeds which can be used at restoration works. Second, according to indoor experiment, as the depth of topsoil used thicker, the number of plants and coverage rates were increased. Also, there were no distinct differences in coverage rates between 5cm and 8cm thick topsoil plots and it can be possible to use at least 3cm thick topsoils even thou lower coverage rates. Third, as the results of research about indoor and outdoor experimental plots, there were difference in plant emergency. If we use the stabilizer to protect soil erosion, then we can get more increased results at sloped outdoor experimental plots. Fourth, based on environmental impact assessment, there was 389 species in whole region of Mt. GARIWANG. Through this seed bank experiments, we can find 23 varieties, including 4 tree varieties and 19 herbaceous varieties. We can find lots of native herbaceous species from topsoils. Fifth, this research was done at the specific area of Mt. GARIWANG and used limited topsoils. But we monitoring more longer periods, then there will be more useful outcomes. Finally by researching topsoils of Mt. GARIWANG, we can find diverse native plants. Thus, we must reuse natural topsoils of Mt. Gariwang for restoration of original vegetation.

• 복식문화연구
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• 제7권6호
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• pp.98-115
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• 1999

As sports infiltrates each field of modern society and becomes familiar, sports wear was changed functionally and was introduced and enveloped into everyday dress. Modern sports wear has been the settled in everyday life deeply and become the clothes of life which are worn regardless of place, time and age. The aim of this paper was to clarify what kind of fashion of sports style would be given to the modern people. In this study, sports wear which has become everyday dress classified from a gender point of view. It was divided into masculine image, feminine image, and neutral image. Sports wear of masculine image generated a silhouette which emphasized the shoulder with the aspiration for youth and health. Wide shoulder was considered as the symbol of masculine beauty and the expression of healthy beauty. It was reflected well in body conscious look. And owing to the development of up-to-data materials, innovation of design, and the study of human body technology, the functional character was settled in the sports wear which showed masculine image. Sports wear of feminine image was represented fashion of body exposure, body feet with body conscious look, and romantic mode. This image was expressed fashion as comforts, pleasant, active design, materials, color, and romantic feminine beauty. Sports wear of neutral image was expressed into unisex clothes. This cloths have no difference in gender, age, and class. It was used as casual sports wear. In the 1960s, young generation participated in such street sports as street basketball and skate. They usually sore the sports wears of neutral image such as cycling, skating, and ski. In the materials of sports, the development of up-to-data material like lycra made the sayings lifelike, “up-to-data material is the second skin” It show that glamorous feminine image and strong masculine image coexisted. The contemporary concept of sportswear is no longer limited to those clothes for sports found in such places like tennis court or swimming pool. Now, the sports wear become more like casual activity wear all classes of people can enjoy in their life regardless of where they are, when they wear, and even how old they are.

• 멤브레인
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• 제7권1호
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• pp.39-47
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• 1997

자체적으로 설계한 막분리 장치 시스템을 사용하여 먼저 순수한 물(3차 처리수)을 대상으로 polysulfone 재질의 중공사형 한외여과막에 대한 분리 성능을 조사하였다. 분획분자량(Molecular cutoff) 5,000 및 10,000 두 종류의 한외여과막에 대하여 실험한 결과, 온도가 증가함에 따라 공급량에 대한 투과량의 비인 회수율이 증가하는 경향을 보였다. 또한, 다양한 분자량의 polyethylene glycol 및 dextran 2,000 ppm 수용액으로 분획분자량을 확인한 결과, 표시된 값보다 다소 큰 값을 얻을 수 있었다. 이러한 기초 실험결과를 토대로 하여, 최종적으로 생활하수의 유입으로 수질이 악화되고 있는 춘천시 공지천의 물을 처리 대상으로 선정하여 원수의 성분을 분석하고, 한외여과 실험을 실시하였다. 그 결과, 생물학적 산소요구량(BOD) 및 총고형물(TS), 탁도가 모두 처리수에서 원수보다 탁월한 감소를 나타내었다. 따라서, 중공사형 한외여과막을 사용한 생활하수 처리에 가능성을 본 연구를 통하여 확인할 수 있었다.

• 대한물리의학회지
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• 제11권1호
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• pp.61-69
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• 2016

PURPOSE: This study was conducted to investigate the effect of the virtual reality training (VRT) using 3-dimensional video gaming technology on spatiotemporal gait parameters in older adults. METHODS: The study participants were divided into two groups: the VRT group and the control group. Those in the VRT group were enrolled in a VRT, which was conducted for 60 min per day, two times a week, during the 6-week research. The Wii-Fit balance board game was used for the VRT intervention. The VRT consisted of 6 different types of games, namely, jogging, swordplay, ski jump, hula hoop, tennis, and step dance. A 3-dimensional TV was used for 3-dimensional display. Participants in both the groups received 3 sessions of fall prevention education, at the first, third, and fifth weeks. Their gait parameters were measured by using OptoGait. RESULTS: After 6 weeks of the VRT, the spatial gait parameters of the participants, that is stride length and step length, were significantly improved compared with those of the control group participants (p<0.05). The temporal gait parameters, such as velocity, cadence, stride time, and step time, also showed improvement after the completion of the VRT training (p<0.05). Both the temporal and spatial gait parameters of the VRT group participants showed improvement after 6 weeks of the program compared with those of the control group participants (p<0.05). CONCLUSION: The VRT using 3-dimensional video gaming technology might be beneficial for improving gait parameters to prevent falls among older adults.

• KSBB Journal
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• 제16권6호
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• pp.579-591
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• 2001

The cell growth inhibitor effect of cervical cancer cells was investigated by liposome mediated transfection (pRcCMVp53/lipofectin) and by transfection using adenovirus (AdCMVp57). The papilloma virus cancer cell lines we used in this study were HPV16 positive, having inhibiter gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3. LacZ gene of E.coli was used as the marker gene for the transfection efficiency. The effect on the inhibition of tumor cell growth was measured by cell count and cell viability though ELISA analysis and MTT assay. The inhibition of tumor cell growth was confirmed by measuring each assay for six days, comparing with the normal control cell growth. The cell growth of cervical cancer calls by transfection was significantly reduced and showed tittle differences among the cell lines. To eliminate the potential problem of Ad(adenovirus) contamination during rAAV production, rAAV can be produced by a triple transfection of vector plasmic, packaging plasmid, and adenovirus helper plasmid. To examine the helper functions of Ad plasmids on the production of rAAV vector, we carried out cotransfection of three plasmids, AAV vector, packaging construct, and Ad helper plasmids. The optimized transfection condition for calcium phosphate method is 25ug of total DNA per 10-cm-diameter plate of 293 cell. We found that rAAV yields peaked at 48hr after Ad infection. The titer of rAAV was measured by the dot blot analysis to measure the number of particles/ml based on the quantification of viral DNA. Recent1y, Kombucha(fungi) was identified as a very potent antileukefic agent. In the present study, effect of natural toxin(plankton) and Kombucha is PSP(GTXI-3, neoSTX), on various MTT assay cervical cancer cell line. Toxin(GTX 1-3, neoSTX) also inhibited the proliferation in primary cervical cancer calls in a dose-dependent toxin concentration. These results showed that toxin was very potent in inhibiting the proliferation of cervical cancer calls in vitro. Toxins and Kombuoha exhibited a dose dependent inhibition of cellular proliferation in cancer cell line.

• Asian Pacific Journal of Cancer Prevention
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• 제16권1호
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• pp.169-174
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• 2015

Cervical cancer (CxCa) is the most common cancer in women and a prominent cause of cancer mortality worldwide. The primary cause of CxCa is human papillomavirus (HPV). Radiation therapy and chemotherapy have been used as standard treatments, but they have undesirable side effects for patients. It was reported that gallic acid has antioxidant, antimicrobial, and anticancer activities. Gold nanoparticles are currently being used in medicine as biosensors and drug delivery agents. This study aimed to develop a drug delivery agent using gold nanoparticles conjugated with gallic acid. The study was performed in uninfected (C33A) cervical cancer cells, cervical cancer cells infected with HPV type 16 (CaSki) or 18 (HeLa), and normal Vero kidney cells. The results showed that GA inhibited the proliferation of cancer cells by inducing apoptosis. To enhance the efficacy of this anticancer activity, 15-nm spherical gold nanoparticles (GNPs) were used to deliver GA to cancer cells. The GNPs-GA complex had a reduced ability compared to unmodified GA to inhibit the growth of CxCa cells. It was interesting that high-concentration ($150{\mu}M$) GNPs-GA was not toxic to normal cells, whereas GA alone was cytotoxic. In conclusion, GNPs-GA could inhibit CxCa cell proliferation less efficiently than GA, but it was not cytotoxic to normal cells. Thus, gold nanoparticles have the potential to be used as phytochemical delivery agents for alternative cancer treatment to reduce the side effects of radiotherapy and chemotherapy.

• 대한의생명과학회지
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• 제3권2호
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• pp.71-76
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• 1997

실험적 자궁암 치료제로서 본 연구소에서 합성한 마이토센유사체를 인체 자궁암세포주인 SiHa, C33A, HeLa, CaSki에 처리하여, 체외 항암활성 측정시 합성 마이토센유사체들은 이 들 세포주에 대하여 대조 항암제들에 비하여 의의있는 세포독성을 보였으며, SiHa 세포주를 이용한 종양클론형성 억제검사에서는 22번 화합물만이 종양성장 억제활성이 가장 우수하였고 다른 화합물들은 대조항암제들에 비하여 활성이 낮았다. 이 마이토센유사체들에 대한 체외 항암감수성결과는 향후 전임상적인 자궁경부암 화합요법제의 기초자료로 유용할것으로 생각된다.

• 대한한의학회지
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• 제26권3호
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• pp.43-54
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• 2005

Backgrounds : Many people think that ski disease has littel influence on the quality of life in patient, but various studios show that skin disease may produce a range of impacts on QOL. Nevertheless, there is much to be learned about the association between the duality of life and skin disease in the area of Oriental dermatology. Objectives : This study was conducted fe investigate the impact of the various skin diseases on QOL and explore prognostic factors that influence outcomes. Methods : 103 patients were recruited who filled out more than $90\%$ of the skindex-29 questionnaire. The function of sum scores and three domain scores with each skin disease were investigated, as well as the correlation of each domain scores. Results : The disease with the lowest qualify of life was atopic dermatitis. followed by eczematous dermatitis, seborrheic dermatitis and acne vulgaris in order. The sum scales showed significant correlation with three domain scores in atopic dermatitis, urticaria, allergodermia, acne vulgaris, psoriasis and seborrheic dermatitis, The correlation of all three domain scores was significant in atopic dermatitis, urticaria, allergodermia, and psoriasis. Conclusions : Tn this study, skin disease affects the quality of life in physical, social, and psychological factors- Thus, we must consider the quality of life as well as clinical severity inpatients with skin disease.

• KSBB Journal
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• 제5권3호
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• pp.269-277
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• 1990

메탄올이용균인 Methylobacillus sp. SKI의 균체생산효율을 증대시킬 목적으로 마이크로컴퓨터 제어 배양기를 이용해 고농도 유가배양을 수행하였다. 배양액의 초기 메탄올농도를 1.0%(v/v)로 하여 회분배양할 경우 배양 13시간만에 2.07g/l의 균체농도에 도달하였다. 공기 공급에 의한 유가배양에서 최대교반속도 1200rpm, 최대 공기유량 5.0$\ell$/min의 조건으로 배양시 약 15시간만에 13.7$\ell$/ldml 균체농도에 도달하였다. 산소공급에 의한 유가배양에서 최대교반속도 1200rpm, 공기유량 1.0$\ell$min, 최대산소유량 5.0$\ell$/min의 조건으로 배양시 17시간만에 45.3g/l의 균체농도에 도달하였다. 균의 대수 증식기를 공기공급에 의한 유가 배양으로 회분배양에 비해 약 3시간, 산소공급에 의한 유가배양으로 회분배야에비해 약 3시간, 산소공급에 의한 유가배양으로 회분배양에 비해 약 4시간 더 연장할 수 있었다. 즉, 유가배양로 단시간에 높은 농도의 균체를 얻은 것은 feedback제어에 의해 메탄올을 저해농도 이하로 유지시키면서 용존산소를 한계농도 이상으로 제어함으로써 균의 대수적 증식으로 연장시킨 결과이다. 산소공급에 의한 유가배양중 균체농도 27.6g/l에서 미량원소성분이 결핍되었고, 42.8g/l에서 $Mg^2^+$성분이 결핍되었으므로 배양중에 추가공급되었다.