• Microbiology and Biotechnology Letters
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• v.15 no.5
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• pp.319-327
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• 1987

In order to develop useful plasmid vectors for Zymomonas cells, attempts were made to isolate natural plasmids from Z. mobilis ATCC10988. Among a few plasmids isolated, a small plasmid of 3.9 Kb size was chosen and designated as pZM3. By introducing the replication origin of pZM3 into pBR325, a hybrid plasmid vector of 8.4 Kb size, pHZ22, was constructed. This vector contained chloramphenicol resistant gene as a selectable marker and proved to be conjugally transmissible and stably maintained in Z. mobilis. Tetracycline resistant gene was isolated from RP4 and introduced into pHZ22 to make a new vector called pHZT224 of 10.7 Kb size. Through n series of experiments, it was evident that these plasmid vectors containing selectable markers of chloramphenicol and tetracycline resistance were shuttle vectors functional in Z. mobilis as well as E. coli.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10b
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• pp.21-21
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• 2003

An efficient plant regeneration system was developed for Hordeum vulgare L. cv Baegdong - an important Korean cultivar. The protocol was based on a series of experiments involving the sizes of mature embryos and the culture media. The embryo size is found to be critical for the establishment of embryogenic callus. Embryos of 1.1-1.5 mm size showed a much higher ability to produce embryogenic callus capable of regenerating green plants. The auxins picloram and dicamba proved effective in inducing callus from mature embryos. 2.5 mg $I^{-1}$ dicamba and 4.0 mg $I^{-1}$ picloram in Murashige and Skoog's (MS) medium was optimum for the induction of primary callus. The induced primary callus was loose and friable which ultimately developed into creamy white and compact callus after transferring into the fresh medium. Multiple shoots were induced in the MS medium supplemented with 6.0 g $I^{-1}$ maltose, 20 mg $I^{-1}$ sorbitol, 0.5 mg $I^{-1}$ 2,4-D and 1.0 mg $I^{-1}$ kinetin and the rate was 6.5 shoots per embryo. Regenerated plants were hardy and developed roots rapidly in the medium containing 0.2 $I^{-1}$ IBA. This efficient plant regeneration system provides a foundation for generating transgenic plants of this important barley cultivar.

• The Korean Journal of Malacology
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• v.21 no.1
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• pp.47-56
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• 2005

A series of radiotracer experiments were employed to quantitatively compare the biokinetics of uptake from the dissolved phase (influx rates), uptake from the various types of food source (assimilation efficiency), and loss (efflux) of Ag between Potamocorbula amurensis and Macoma balthica. Simultaneously, influx rates of dissolved Cd in both clams were determined to compare with those of Ag. Effects of salinity on influx rates were evaluated in these 2 euryhaline species, as were effects of clam size. Influx rate of Ag and Cd (${\mu}g g^{-1}$ [dry wt.] $d^{-1}$) increased linearly with metal concentrations. Influx rates of Ag in both clams were 3 to 4 times those of Cd. Absolute influx rates of the 2 metals were 4 to 5 times greater in P. amurensis than M. balthica, probably because of differences in biological attributes (i.e. clearance rate or gill surface area). As salinity was reduced from 20 to 2.5 psu, the influx rate of Cd in P. amurensis increased 4-fold and that of Ag increased 6-fold, consistent with expected changes in speciation. Weight-specific metal influx rates (${\mu}g g^{-1}$ [dry wt.] $d^{-1}$) were negatively correlated with the tissue dry weight of the clams, but most rate constants determining physiological turnover of assimilated metals were not affected by clam size.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.39.4-40
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• 2017

According to the Korean government's Long-term Space Development Plan 2040, "Creative space science research" is included in a statement to investigate the origin and evolution of the universe by conducting a series of Korean space telescope missions: launch of space telescopes on a small satellite and an international collaboration explorer by 2020, a mid-size domestic space telescope by 2030, and a large size Korea leading international space telescope by 2040. We studied the feasibility of the future Korean Space Telescope (KST) for a mid-size domestic satellite platform. In order to pursue the uniqueness of the science program, we consider a wide range of observing wavelength (0.2um ~ 2.0um) with a spectral resolution of R~6 in the NUV and optical bands, and R~30 for NIR, utilizing an off-axis TMS(Three Mirror System) optics with a wide field of view ($2{\times}4$ degrees) which is optimized for ultra-low surface brightness sources. The main science goals of the mission include investigations of the galaxy formation, cosmic web, and the cosmic background radiation in the NUV-NIR regions. In this paper, we present the science cases and several technical challenges to be resolved along with the future milestones for the success of the KST mission.

• Magazine of the Korea Concrete Institute
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• v.11 no.1
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• pp.161-171
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• 1999

Impact resonance testing was carried out on small and large concrete blocks containing several types of artificial flaws respectively. Quantitative analysis of the observed peak frequencies in the impact resonance tests identifies the possible normal modes of concrete blocks containing flaws. and enables to determine the depth and size of the flaws in concrete blocks. In this study, concrete can be treated as a homogeneous and isotropic material. The flaw size and location at each section of artificial flaw series in small and large concrete blocks, determined through two-dimensional scanning of impact point and real-time fast Fourier transform, are in good agreement with real size location, respectively. Consequently, quantitative analysis method of vibration modes in the impact resonance tests, which can be applied for homogeneous and isotropic material, can be useful for the detection of flaws in any case of small and large concrete blocks in this study.

• Earthquakes and Structures
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• v.11 no.5
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• pp.823-840
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• 2016

The plastic hinge lengths of beams and columns are a critical demand parameter in the nonlinear analysis of structures using the finite element method. The numerical model of a plastic hinge plays an important role in evaluating the response and damage of a structure to earthquakes or other loads causing the formation of plastic hinges. Previous research demonstrates that the plastic hinge length of reinforced concrete (RC) columns is closely related to section size, reinforcement ratio, reinforcement strength, concrete strength, axial compression ratio, and so on. However, because of the limitations of testing facilities, there is a lack of experimental data on columns with large section sizes and high axial compression ratios. In this work, we conducted a series of quasi-static tests for columns with large section sizes (up to 700 mm) and high axial compression ratios (up to 0.6) to explore the propagation of plastic hinge length during the whole loading process. The experimental results show that besides these parameters mentioned in previous work, the plastic hinge of RC columns is also affected by loading amplitude and size effect. Therefore, an approach toward considering the effect of these two parameters is discussed in this work.

• Journal of the Korea institute for structural maintenance and inspection
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• v.7 no.2
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• pp.177-184
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• 2003

A series of experimental work has been conducted to evaluate the capability of Ground Penetrating Radar (GPR) system in detecting delamination inside concrete. Three antenna at 900 MHz, 1000 MHz, and 1500 MHz frequency are used in the experiments for laboratory size specimens, and 400 MHz antenna has been used for a large size specimen. The laboratory size specimens have the dimensions of 1,000 mm (length) ${\times}$ 600 mm (width) ${\times}$ 140 mm (thickness) with a delamination of 200 mm (length) ${\times}$ 600 mm (width) ${\times}$ 140 mm (thickness). The cover depth of the delamination is varied as follows: 20 mm, 30 mm, 60 mm, and 70 mm. In all cases, the delamination has been successfully identified. The property of three frequencies was seized about detecting delamination. Also, it was shown that the image results in GPR were improved by signal processing.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.4
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• pp.134-139
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• 2008

A spirally arrayed nano-pattern is designed as a model pattern for the next generation optical storage media. The pattern consists off types of embossed rectangular dot, which are 50nm, 100nm, 150nm and 200nm in length and 50nm in width. The height of the dot is designed to be 50nm. The pitch of the spiral track of the pattern is 100nm. A ER(Electron resist) master for this pattern is fabricated by e-beam lithography process. The ER is first spin-coated to be 50nm thick on a Si wafer and then the model pattern is written on the coated ER layer by e-beam. After developing this pattern written wafer in the solution, a ER pattern master is fabricated. The most conventional e-beam machine can write patterns in orthogonal way, so we made our own pattern generator which can write the pattern in circular or spiral way. This program generates the patterns to be compatible with the e-beam machine from Raith(Raith 150). To fabricate 50nm pattern master precisely, a series of experiments were done including the design compensation for the pattern size, optimization of the dose, acceleration voltage, aperture size and developing. Through these experiments, we conclude that the higher accelerating voltages and smaller aperture size are better for mastering the nano pattern which is in order of 50nm. With the optimized e-beam lithography process, a spiral arrayed 50nm pattern master adopting PMMA resist was fabricated to have dimensional accuracy over 95% compared to the designed. Using this pattern master, a metal pattern stamp will be fabricated by Ni electro plating for injection molding of the patterned plastic substrate.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.52 no.4
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• pp.281-289
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• 2016

Spinyhead sculpin Dasycottus setiger, a species of cold water fish, is distributed along the eastern coastal waters of Korea. A series of fishing experiments was carried out in the waters near Uljin from June, 2002 to November, 2004, using the experimental monofilament gill nets of different mesh sizes (82.2, 89.4, 104.8, and 120.2 mm) to describe the selectivity of the gill net for the fish. The SELECT (Share Each Length's Catch Total) analysis with maximum likelihood method was applied to fit the different functional models (normal, lognormal, and bi-normal models) for selection curves to the catch data. The bi-normal model with the fixed relative fishing intensity was selected as the best-fit selection curve by AIC (Akaike's Information Criterion) comparison. For the best-fit selection curve, the optimum relative length (the ratio of fish total length to mesh size) with the maximum efficiency and the selection range ($R_{50%,large}-R_{50%,small}$) of 50% retention were obtained as 2.363 and 0.851, respectively. The ratios of body girth to mesh perimeter at 100% retention where the selection curve of each mesh size represented the optimum total length were calculated as the range of 0.86 ~ 0.87.

• Journal of the Korean Geosynthetics Society
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• v.12 no.1
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• pp.51-61
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• 2013

This paper presents results from a study undertaken to quantitatively evaluate the geotextile pore sizes using optical image analysis. The evaluation was conducted by observing surfaces of coupons cut from resin-impregnated specimens of geotextile-geomembrane layered under various load conditions. Stereological concepts were applied to collect representative specimens from a series of laboratory tests. The sizes of voids enclosed by filaments were expressed in terms of the largest inscribing opening size (LIOS) distribution. The opening diameter corresponding to the 50% cumulative frequency decreased by about 45mm as the load increased from 10 to 300kPa and recovered to about 90% of its initial state on unloading back to 10kPa. The average void size was reduced by 32 and 16.5% as the geotextile was sheared against a textured geomembrane under normal stresses of 100 and 300kPa, respectively. The results showed how the LIOS distribution varied as a function of normal stress and interface shear displacement against a smooth and a textured geomembrane surfaces.