• Journal of Korean Society of Forest Science
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• 제88권2호
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• pp.273-281
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• 1999

In this study, linear programming (LP) was applied to solving for optimal harvesting schedules of multiple-use forest management in Mt. Kari area managed by Chunchun National Forest Station. Associated with the geographic characteristics, the study area was classified into 4 large management units or watersheds and simultaneously applied were the site-specific levels of management constraints : nondeclining yield, initial cut for existing stands, % cut area, the volume of soil erosion, timber production and carbon storage, ending inventory condition and % area species selection for regeneration. The problem was formulated using both Model I and Model II techniques. In this paper, the formulations are presented and the results of the optimal solutions are discussed for comparison purposes.

• Journal of Korean Society of Transportation
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• 제14권2호
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• pp.119-136
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• 1996

The evaluation of risk level or possibility of traffic accidents is a fundamental task in reducing the dangers associated with current transportation system. However, due to the lack of data and basic researches for identifying such factors, evaluations so far have been undertaken by only the experts who can use their judgements well in this regard. Here comes the motivation this thesis to evaluate such risk level more or less in an automatic manner. The purpose of this thesis is to test the fuzzy-logic theory in evaluating the risk level of traffic accidents. In modeling the process of expert's logical inference of risk level determination, only the geometric features have been considered for the simplicity of the modeling. They are the visibility of road surface, horizontal alignment, vertical grade, diverging point, and the location of pedestrain crossing. At the same time, among some inference methods, fuzzy composition inference method has been employed as a back-bone inference mechanism. In calibration, the proposed model used four sites' data. After that, using calibrated model, six sites' risk levels have been identified. The results of the six sites' outcomes were quite similar to those of real world other than some errors caused by the enforcement of the model's output. But it seems that this kind of errors can be overcome in the future if some other factors such as driver characteristics, traffic environment, and traffic control conditions have been considered. Futhermore, the application of site's specific time series data would produce better results.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1827-1834
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• 2015

The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the binding site groove, on the function of SMG1 lipase. Site-directed mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast Pichia pastoris, purified, and characterized for their activity toward DAG and p-nitrophenol (pNP) ester. Compared with wild-type SMG1, F278A retained approximately 78% of its activity toward DAG, but only 11% activity toward pNP octanoate (pNP-C8). L103G increased its activity on pNP-C8 by approximately 2-fold, whereas F104G showed an approximate 40% decrease in pNP-C8 activity, and they both showed decreased activity on the DAG emulsion. The deletion of 103-104 retained approximately 30% of its activity toward the DAG emulsion, with an almost complete loss of pNP-C8 activity. The deletion of 103-104 showed a weaker penetration ability to a soybean phosphocholine monolayer than wild-type SMG1. Based on the modulation of the specificity and activity observed, a pNP-C8 binding model for the ester (pNP-C8, N102, and F278 form a flexible bridge) and a specific lipid-anchoring mechanism for DAG (L103 and F104 serve as "anchors" to the lipid interface) were proposed.

• International Area Studies Review
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• 제15권1호
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• pp.571-583
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• 2011

We use the properties of competitive location equilibrium to study the relationship between the demand for education and the choice of primary residential location. Consumers can work and live in a comparatively high wage place where there are few education opportunities, or live in a place where education is available and commute to work. If education and employment are each location-specific, there are pooling equilibria in which consumers locate according to their preference for education. In general, the stronger the taste for education, the greater the attraction of living close to the education site and the lower the demand for other goods, including housing. Exploring the effects of the model parameters on the spatial distribution of consumers, we find that a higher frequency of trips taken to the education site, a shorter distance between the work place and the education site, or a greater out-of-pocket education cost each leads to a wider range of consumer types selecting to reside at the education location. We also find that a higher wage lowers the range of consumers who select to live near the education site.

• Korean Journal of Agricultural and Forest Meteorology
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• 제9권4호
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• pp.247-259
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• 2007

In this study, we examined the flux of carbon and water using an eco-hydrological model, Regional Hydro-Ecologic Simulation System (RHESSys). Our purposes were to develop a set of parameters optimized for a well-designed experimental watershed (Gwangneung Research Watershed, GN) and then, to test suitability of the parameters for predicting carbon and water fluxes of other watershed with different regimes of climate, topography, and vegetation structure (i.e Gangseonry Watershed in Mt. Jumbong, GS). Field datasets of stream flow, soil water content (SWC), and wood biomass product (WBP) were utilized for model parameterization and validation. After laborious parameterization processes, RHESSys was validated with the field observations from the GN watershed. The parameter set identified at the GN watershed was then applied to the GS watershed in Mt. Jumbong, which resulted in good agreement for SWC but poor predictability for WBP. Our study showed that RHESSys simulated reliable SWC at the GS by adjusting site-specific porosity only. In contrast, vegetation productivity would require more rigorous site-specific parameterization and hence, further study is necessary to identify primary field ecophysiological variables for enhancing model parameterization and application to multiple watersheds.

• Journal of the Korean Institute of Landscape Architecture
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• 제30권3호
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• pp.1-11
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• 2002

The purpose of this study is to construct an evaluation criteria based on the relative importance and various combinations of each evaluation index and then to appraise urban biotope in terms of nature experience and recreation. After the standard of evaluation had been developed through the review of existing literature, the relative importance of the evaluation index through expert survey was determined, and the evaluation model to assess biotope value was established. The results of this study are as follows; 1) We have constructed the seventeen evaluation criteria through literature reviews, and have provided relative importance to each evaluation index of the natural in biotope, the diversity of landscape patterns, the appearance-existence of water space, the special landscape elements, and the access based on the results of expert survey. and then we have constructed an evaluation system using the relative importance criteria. 2) To evaluate urban biotope using the evaluation system constructed, we selected 14 sites. The final evaluation results of each site is as follows; The first grade areas are the water space with semi-natural style in front of Gangchon Woobang apartments, the forest zone near Page Temple at Mt. Palgong, the edge area of Jisan and Gosan and the tendril yard against Anshim middle school. The second grade areas are Hyomyung elementary school, the farm land behind the third Anshim Jugong apartment, Ehyun put and the swampy land in front of the Kangchon and Anshim construction site. The third grade areas are Seohan and Hwasung high-rise apartments at Beummul-Dong, the Korea Manpower Agency in the Sungseo industrial zone, and the adjacent area of St. Tongil. The only fourth grade area is the commercial zone near Taegu Department Store. 3) It is highly useful to evaluate urban biotope using the evaluation criteria constructed for this research, the evaluation criteria we developed may be difficult to encompass all expert opinion and the period of space-formation is difficult to estimate urban biotopes. In the future, it is necessary to gather the basic data suitable to estimate biotope and it is necessary to develop detailed evaluation criteria applicable to estimate every biotope that has specific property.

• Proceedings of the Korean Society of Crop Science Conference
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• 한국작물학회 2005년도 국제학술회의
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• pp.57-74
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• 2005

Rice yield and protein content have been shown to be highly variable across paddy fields. In order to characterize this spatial variability of rice within a field, the two-year experiments were conducted in 2002 and 2003 in a large-scale rice field of $6,600m^2$ In year 2004, an experiment was conducted to know if prescribed N for site-specific fertilizer management at panicle initiation stage (VRT) could reduce spatial variation in yield and protein content of rice while increasing yield compared to conventional uniform N topdressing (UN, ,33 kg N/ha at PIS) method. The trial field was subdivided into two parts and each part was subjected to UN and VRT treatment. Each part was schematically divided in $10\times10m$ grids for growth and yield measurement or VRT treatment. VRT nitrogen prescription for each grid was calculated based on the nitrogen (N) uptake (from panicle initiation to harvest) required for target rice protein content of $6.8\%$, natural soil N supply, and recovery of top-dressed N fertilizer. The required N uptake for target rice protein content was calculated from the equations to predict rice yield and protein content from plant growth parameters at panicle initiation stage (PIS) and N uptake from PIS to harvest. This model equations were developed from the data obtained from the previous two-year experiments. The plant growth parameters for this calculation were predicted non-destructively by canopy reflectance measurement. Soil N supply for each grid was obtained from the experiment of year 2003, and N recovery was assumed to be $60\%$ according to the previous reports. The prescribed VRT N ranged from 0 to 110kg N/ha with average of 57kg/ha that was higher than 33kg/ha of UN. The results showed that VRT application successfully worked not only to reduce spatial variability of rice yield and protein content but also to increase rough rice yield by 960kg/ha. The coefficient of variation (CV) for rice yield and protein content was reduced significantly to $8.1\%\;and\;7.1\%$ in VRT from $14.6\%\;and\;13.0\%$ in UN, respectively. And also the average protein content of milled rice in VRT showed very similar value of target protein content of $6.8\%$. Although N use efficiency of VRT compared to UN was not quantified due to lack of no N control treatment, the procedure used in this paper for VRT estimation was believed to be reliable and promising method for managing within-field spatial variability of yield and protein content. The method should be received further study before it could be practically used for site-specific crop management in large-scale rice field.

• Journal of Radiation Protection and Research
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• 제29권2호
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• pp.73-90
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• 2004

The Reg. Guide 1.109 model was reviewed against its applicability to calculating radionuclide concentrations in agricultural products for operating nuclear facilities and an improved method was proposed. The model was so modified that the radionuclides deposited since the start of operation could be considered in assessing the root uptake. Translocation factors were introduced in the equation for calculating the concentrations in edible parts due to direct plant deposition. Values specific to Korea were set up for the input parameters of the modified model. The concentrations of $^{54}Mn,\;^{60}Co,\;^{90}Sr\;and\;^{137}Cs$ in rice seeds, Chinese cabbage and radish root were calculated for various hypothetical deposition histories using the Reg. Guide 1.109 model and the modified model with parameter values in the guide and those specific to Korea put in alternately. Through comparisons among the results, it could be expected that the use of the modified model with the input of parameter values specific to Korea would result In a more resonable and realistic assessment.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.45-45
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• 2017

On-site monitoring of vegetable growth parameters, such as leaf length, leaf area, and fresh weight, in an agricultural field can provide useful information for farmers to establish farm management strategies suitable for optimum production of vegetables. Unmanned Aerial Vehicles (UAVs) are currently gaining a growing interest for agricultural applications. This study reports on validation testing of previously developed vegetable growth estimation models based on UAV-based RGB images for white radish and Chinese cabbage. Specific objective was to investigate the potential of the UAV-based RGB camera system for effectively quantifying temporal and spatial variability in the growth status of white radish and Chinese cabbage in a field. RGB images were acquired based on an automated flight mission with a multi-rotor UAV equipped with a low-cost RGB camera while automatically tracking on a predefined path. The acquired images were initially geo-located based on the log data of flight information saved into the UAV, and then mosaicked using a commerical image processing software. Otsu threshold-based crop coverage and DSM-based crop height were used as two predictor variables of the previously developed multiple linear regression models to estimate growth parameters of vegetables. The predictive capabilities of the UAV sensing system for estimating the growth parameters of the two vegetables were evaluated quantitatively by comparing to ground truth data. There were highly linear relationships between the actual and estimated leaf lengths, widths, and fresh weights, showing coefficients of determination up to 0.7. However, there were differences in slope between the ground truth and estimated values lower than 0.5, thereby requiring the use of a site-specific normalization method.

• Molecules and Cells
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• 제44권7호
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• pp.500-516
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• 2021

Cardiac hypertrophic signaling cascades resulting in heart failure diseases are mediated by protein phosphorylation. Recent developments in mass spectrometry-based phosphoproteomics have led to the identification of thousands of differentially phosphorylated proteins and their phosphorylation sites. However, functional studies of these differentially phosphorylated proteins have not been conducted in a large-scale or high-throughput manner due to a lack of methods capable of revealing the functional relevance of each phosphorylation site. In this study, an integrated approach combining quantitative phosphoproteomics and cell-based functional screening using phosphorylation competition peptides was developed. A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. Cell-based functional screening against 18 selected phosphorylation sites identified three phosphorylation sites (Ser-98, Ser-179 of Ldb3, and Ser-1146 of palladin) displaying near-complete inhibition of cardiac hypertrophic growth of NRVMs. Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. Our integrated approach can be used to identify functionally important phosphorylation sites among differentially phosphorylated sites, and unlike conventional approaches, it is easily applicable for large-scale and/or high-throughput analyses.