• Title/Summary/Keyword: site specific

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Chemical Modification of Serratia marcescens Catabolic ${\alpha}-Acetolactate$ Synthase

  • Joo, Han-Seung;Kim, Soung-Soo
    • BMB Reports
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    • v.31 no.2
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    • pp.139-143
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    • 1998
  • The catabolic ${\alpha}-acetolactate$ synthase purified from Serratia marcescens ATCC 25419 was rapidly inactivated by the tryptophane-specific reagent, N -bromosuccinimide, and the arginine-specific reagent, phenylglyoxal. The enzyme was inactivated slowly by the cysteine-specific reagent N-ethylmaleimide. The second-order rate constants for the inactivation by N-bromosuccinimide, phenylglyoxal. and N -ethylmaleimide were $114,749M^{-1}min^{-1}$, $304.3M^{-1}min^{-1}$, and $5.1M^{-1}min^{-1}$, respectively. The reaction order with respect to N-bromosuccinimide, phenylglyoxal, and N-ethylmaleimide were 1.5,0.71, and 0.86, respectively. The inactivation of the catabolic aacetolactate synthase by these modifying reagents was protected by pyruvate. These results suggest that essential tryptophane, arginine, and cysteine residues are located at or near the active site of the catabolic ${\alpha}-acetolactate$ synthase.

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강변여과수 취수에 따른 지하수위의 계절적인 변동 특성

  • Jeong Jae-Yeol;Ham Se-Yeong;Lee Jeong-Hwan;Kim Hyeong-Su;Ryu Sang-Hon;Kim Tae-Won;Kim Mun-Su
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2006.04a
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    • pp.68-71
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    • 2006
  • Seasonal fluctuation of groundwater level by pumping amount and stream discharge at the riverbank filtrate site adjacent to the Nakdong River in Daesan-Myeon was characterized. Groundwater level fluctuation shows increase in wet season (June, July, August and September) and decrease in dry season (the other months). Seasonal variation of pumping amount shows similar trend to the groundwater fluctuation due to higher consumption of potable water in summer. The relation of specific capacity, Nakdong River and pumping quantity was analyzed. The logarithmic relationship between specific capacity and the stream discharge gives high correlation coefficient, 0.96. This fact indicates that the increase of stream discharge rate reduces the rate of drawdown in the pumping area in wet season.

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Site-Specific Growth of Width-Tailored Graphene Nanoribbons on Insulating Substrates

  • Song, U-Seok;Kim, Su-Yeon;Kim, Yu-Seok;Kim, Seong-Hwan;Lee, Su-Il;Song, In-Gyeong;Jeon, Cheol-Ho;Park, Jong-Yun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.612-612
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    • 2013
  • The band-gap opening in graphene is a key factor in developing graphene-based field effect transistors. Although graphene is a gapless semimetal, a band-gap opens when graphene is formed into a graphene nanoribbon (GNR). Moreover, the band-gap energy can be manipulated by the width of the GNR. In this study, we propose a site-specific synthesis of a width-tailored GNR directly onto an insulating substrate. Predeposition of a diamond-like carbon nanotemplate onto a SiO2/Si wafer via focused ion beam-assisted chemical vapor deposition is first utilized for growth of the GNR. These results may present a feasible route for growing a width-tailored GNR onto a specific region of an insulating substrate.

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Site-Specific Growth of Width-Tailored Graphene Nanoribbons on Insulating Substrates

  • Song, U-Seok;Kim, Yu-Seok;Jeong, Min-Uk;Park, Jong-Yun;An, Gi-Seok
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.145.2-145.2
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    • 2013
  • The band-gap opening in graphene is a key factor in developing graphene-based field effect transistors. Although graphene is a gapless semimetal, a band-gap opens when graphene is formed into a graphene nanoribbon (GNR). Moreover, the band-gap energy can be manipulated by the width of the GNR. In this study, we propose a site-specific synthesis of a width-tailored GNR directly onto an insulating substrate. Predeposition of a diamond-like carbon nanotemplate onto a SiO2/Si wafer via focused ion beam-assisted chemical vapor deposition is first utilized for growth of the GNR. These results may present a feasible route for growing a width-tailored GNR onto a specific region of an insulating substrate.

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Development of a GIS Application Model for Analyzing Site-Specific Suitability and Investment Efficiency of Major Plantation Species (주요 조림수종의 적지 판정 및 투자효율성 분석을 위한 GIS 응용모델의 개발)

  • Kim, Eui-Gyeong;Kim, Hyung-Ho;Chung, Joo-Sang
    • Journal of the Korean Association of Geographic Information Studies
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    • v.9 no.2
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    • pp.1-10
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    • 2006
  • The objective of this study was to develop a GIS application model for analyzing site-specific suitability and investment efficiency of major plantation tree species. The model was designed to choose the best plantation species in terms of both potential stand growth and investment efficiency. Site index and IRR were used as the criteria for the potential of stand growth and investment efficiency, respectively. In the model, the conditions of forest stand management and site index are estimated as the function of site-environmental factors extracted by a series of spatial analyses of digital maps of FGIS. Based on site index values of tree species, the model screens out all the high potential tree species, in terms of stand growth, as the candidates for species selection and, then, calculates IRR for managing plantation forest stands for all the candidate tree species. The tree species of the highest IRR would be chosen as the one possessing the highest potential in terms of stand growth and profit. The model was applied to a case study for analyzing the site-specific suitability of 6 tree species in Taehwa University Forest of Seoul National University and the results are given in this paper.

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Pass by Noise Test Site Variability

  • Kim Byoung Sam
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.14 no.3
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    • pp.116-121
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    • 2005
  • The objective of this paper is to compare the site-to-site variability of ISO 10844 pass by the noise test sites. In order to investigate the site-to-site variance of test surfaces, European commercial tires are tested at seven different test sites. Three Korea test sites and four Europe test sites are selected. The pass by noise test is done according to a 2001/43/EC regulation. Although the ISO surface has a very specific track composition, it does not reduce the variation of pass by noise measurements over the surface of test sites. This paper shows that the test results of pass by noise level are different depending on the test sites. The correlation obtained in this work is able to predict the pass by noise level for certain test site using the data measured from another test site. The prediction value is range with an error within 1dB(A).

A Phenomenological Study on the Ecological Restoration Design of Human Environment (生態的 環景復元設計에 關한 現象學的 考察 - 마틴 하이데거의 現象學을 中心으로 -)

  • 변찬우
    • Journal of the Korean Institute of Landscape Architecture
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    • v.25 no.3
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    • pp.155-176
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    • 1997
  • Today most landscape architects and scientists have approached ecological restoration, only in a scientific way or on aesthetic way so as to deal with the complex phenomena of a site easily. However, 'the real world' as well as 'ecological nature' as it is can not be totally dealt by the scientific approach which is quantitative and reductioinal. ince a site to be restored has the character of 'place' where man 'dwells', it must be designed and built as total phenomenon. In terms of Martin Heidegger's interpretation of "revealing", we might find out how to overcome the dilemma of the 'modern technology'. To the point of the phenomenological integrity between 'modern technology' and 'art', we can reveal 'sense of place' on a site. The phenomenological approach, which is related to the characteristics of the site, implies something more than scientific since it focuses on the development of site-specific data without wasting extra data for ecological restoration. Bodily experiencing a site with the perception, man discloses the site's nature and he also analyzes it in a scientific way. Therefore the phenomenological approach might be a clue of constructing ecological restoration as well as construing the identity of landscape architecture.

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Characterization of Site-Specific Recombination by the Integrase MJ1 from Enterococcal Bacteriophage ${\Phi}FC1$

  • Park, Mi-Ok;Lim, Ki-Hong;Kim, Tae-Hyung;Chang, Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • v.17 no.2
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    • pp.342-347
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    • 2007
  • Bacteriophage ${\Phi}FC1$ integrase (MJ1) was previously shown to perform a site-specific recombination between a phage attachment site (attP) and a host attachment site (attB) in its host, Enterococcus faecalis, and also in a non-host bacterium, Escherichia coli. Here, we investigated biochemical features of MJ1 integrase. First, MJ1 integrase could perform in vitro recombination between attP and attB in the absence of additional factors. Second, MJ1 integrase interacted with att sites. Electrophoretic mobility shift assays and DNase I footprinting revealed that MJ1 integrase could efficiently bind to all the att sites and that MJ1 integrase recognized relatively short sequences (${\sim}50bp$) containing an overlapping region within attB and attP. These results demonstrate that MJ1 integrase indeed catalyzes an integrative recombination between attP and attB, the mechanism of which might be simple and unidirectional, as found in serine integrases.