• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.44-44
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• 2003

$BK_{Ca}$ channels were suggested to contain one or more domains of the ‘regulator of K+ conductance’(RCK) in their cytosolic carboxyl termini (Jiang et al.2001). It was also shown that the RCK domain in mammalian $BK_{Ca}$ channels might sense the intracellular $Ca^{2+}$ with a low affinity (Xia et al. 2002). We aligned the amino acid sequence of the $\alpha$-subunit of rat $BK_{Ca}$ channels (rSlo) with known RCK domains and identified a second region exhibiting about 50％ homology. This putative domain, RCK2, contains the characteristic amino acids conserved in other RCK domains. We wondered whether this second domain is involved in the domain-domain interaction and the gating response to intracellular $Ca^{2+}$ for rSlo channel, as revealed in the structure of RCK domain of E. coli channel (Jiang et al.2001). In order to examine the possibility, site-directed mutations were introduced into the RCK2 domain of rSlo channel and the mutant channels were expressed in Xenopus oocytes for functional studies. One of such mutation, G772D, in the putative nucleotide-binding domain resulted in the enhanced $Ca^{2+}$ sensitivity and the channel gating of rSlo channel. These results suggest that this region of $BK_{Ca}$ channels is important for the channel gating and may form an independent domain in the cytosolic region of $BK_{Ca}$ channels. In order to obtain the mechanistic insights of these results, G772 residue was randomly mutagenized by site-directed mutagenesis and total 17 different mutant channels were constructed. We are currently investigating these mutant channels by electrophysiological techniques.ical techniques.

• Journal of the Korea Institute of Building Construction
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• v.20 no.1
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• pp.93-102
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• 2020

The purpose of this study is to find out how to measure the stress related to accidents at the construction site promptly and conveniently to prevent safety accidents of construction workers. Accordingly, we analyzed the correlations between the questionnaire tool index that measures the stress associated with complex psychology of humans by integrating emotion, cognition, physical and behavioral responses, and basic brain waves, SEF-90, concentration, stress index from brain wave. As a result, which had the highest correlation with the stress measured through the questionnaire, was the SEF-90, and the regression analysis between two independent variables yielded a specific regression equation. This suggests the possibility of measuring the integrated stress of construction workers through the EEG signal at the construction site, and it can be used for the safety management of the construction site in the future.

• Journal of The Korea Institute of Healthcare Architecture
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• v.23 no.3
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• pp.19-27
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• 2017

Purpose: The characteristics of site plan and space configuration of public dentistry through examining the public dental healthcare centers for the disabled in Korea and comparison between them are necessary for the development of planning of the dental healthcare system. This study has been started to provide basic informations such as nationwide distribution, site relationship, and space configuration for the planning of public dental hospital architecture. Methods: Literature review of publicness and public dental healthcare and investigation on current status of public dental healthcare center for the disabled in Korea have been conducted. The site plan and space configuration of eight public healthcare centers for the disabled have been analyzed. Results: The result of this study can be summarized in three points. The first one is that public dentistry in Korea are distributed public dental hospital for the disabled in Seoul and public dental healthcare centers for the disabled in eight provinces. The second one is that the types of the dental healthcare center for the disabled are divided with remodeling type which is diverted from existed dental or medical out patient clinic space or independent building type which is planned with a new and exclusive usage for the disabled. The third one is that the space configuration of dental healthcare center for the disabled is needed more required programs, larger treatment unit space, and more private clinic space than typical dental treatment plan. Implications: This study is the starting point for the research of public dentistry and it is necessary to analyze the dental prevention and dental public policy to develop the public dental healthcare system.

• Molecules and Cells
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• v.42 no.5
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• pp.418-425
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• 2019

Multicistronic elements, such as the internal ribosome entry site (IRES) and 2A-like cleavage sequence, serve crucial roles in the eukaryotic ectopic expression of exogenous genes. For utilization of multicistronic elements, the cleavage efficiency and order of elements in multicistronic vectors have been investigated; however, the dynamics of multicistronic element-mediated expression remains unclear. Here, we investigated the dynamics of encephalomyocarditis virus (EMCV) IRES- and porcine teschovirus-1 2A (p2A)-mediated expression. By utilizing real-time fluorescent imaging at a minute-level resolution, we monitored the expression of fluorescent reporters bridged by either EMCV IRES or p2A in two independent cultured cell lines, HEK293 and Neuro2a. We observed significant correlations for the two fluorescent reporters in both multicistronic elements, with a higher correlation coefficient for p2A in HEK293 but similar coefficients for IRES-mediated expression and p2A-mediated expression in Neuro2a. We further analyzed the causal relationship of multicistronic elements by convergent cross mapping (CCM). CCM revealed that in all four conditions examined, the expression of the preceding gene causally affected the dynamics of the subsequent gene. As with the cross correlation, the predictive skill of p2A was higher than that of IRES in HEK293, while the predictive skills of the two multicistronic elements were indistinguishable in Neuro2a. To summarize, we report a significant temporal correlation in both EMCV IRES- and p2A-mediated expression based on the simple bicistronic vector and real-time fluorescent monitoring. The current system also provides a valuable platform to examine the dynamic aspects of expression mediated by diverse multicistronic elements under various physiological conditions.

• Journal of the Korean Geotechnical Society
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• v.24 no.3
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• pp.13-23
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• 2008

In a nonlinear site response analysis which is performed in time domain, small strain damping is modeled as viscous damping through use of various forms of Rayleigh damping formulations. Small strain damping of soil is known to be independent of the loading frequency, but the viscous damping is greatly influenced by the loading frequency. The type of Rayleigh damping formulation has a pronounced influence on the dependence. This paper performs a series of nonlinear analyses to evaluate the degree of influence of the viscous damping formulation on Korean soil profiles. Analyses highlight the strong influence of the viscous damping formulation for soil profiles exceeding 30 m in thickness, commonly used in simplified Rayleigh damping formulation overestimating energy dissipation at high frequencies due to artificially introduced damping. When using the full Rayleigh damping formulation and carefully selecting the optimum modes, the artificial damping is greatly reduced. Results are further compared to equivalent linear analyses. The equivalent linear analyses can overestimate the peak ground acceleration even for shallow profiles less than 20 m in thickness.

• Wind and Structures
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• v.6 no.2
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• pp.91-106
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• 2003

The paper presents the results of 1:50 geometrical scale laboratory modeling of wind-induced point pressure on the roof of the Texas Tech University (TTU) test building. The nominal (prevalent at the TTU site) wind and two bounding (low and high turbulence) flows were simulated in a boundary-layer wind tunnel at Colorado State University. The results showed significant increase in the pressure peak and standard deviation with an increase in the flow turbulence. It was concluded that the roof mid-plane pressure sensitivity to the turbulence intensity was the cause of the previously reported field-laboratory mismatch of the fluctuating pressure, for wind normal and $30^{\circ}$-off normal to the building ridge. In addition, it was concluded that the cornering wind mismatch in the roof corner/edge regions could not be solely attributed to the wind-azimuth-independent discrepancy between the turbulence intensity of the approach field and laboratory flows.

• English Language & Literature Teaching
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• v.13 no.3
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• pp.227-251
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• 2007

The development and use of computer mediated communications (CMC) as a tool for teaching and learning English has grown considerably in recent years. The purpose of this study is to investigate factors related to learners' participation, achievement, and satisfaction in EFL classes using web-based bulletin boards. The total number of 77 university students participated in this study. Three domains and eight independent variables investigated in this study were a learner-related domain (attitudes toward CMC, intrinsic motivation, extrinsic motivation, attitudes toward writing), an interaction-related domain (student-student interaction, teacher-student interaction), and an environmental domain (physical support and design of the web site). In order to determine interrelation of variables correlation analysis and multiple regression analysis were used. The results of this study showed that the factors predicting a learner's participation were instrumental motivation, attitudes toward writing, and teacher-student interaction. The factors explaining a learner's achievement were learner's participation and attitudes toward writing, and the factors predicting a learner's satisfaction were integrative motivation, student-student interaction, teacher-student interaction, physical support and learner's participation.

• Proceedings of the KSME Conference
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• 2001.06d
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• pp.189-194
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• 2001

A simultaneous visualization of the behavior of bubbles and dry spots has been carried out for pool boiling of R-1l3 on a horizontal heater. From video imaging and image processing analysis, the formation of bubbles and dry spots occurs simultaneously, and therefore they should be considered as a synchronized concept rather than independent identities. The dry spot density is equivalent to the active site density in the region before CHF. At CHF point, large dry areas due to the coalescence of neighboring dry areas cover the heater surface.

• BMB Reports
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• v.42 no.12
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• pp.823-828
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• 2009

Techniques for analyzing protein-DNA interactions on a genome-wide scale have recently established regulatory roles for distal enhancers. However, the large sizes of higher eukaryotic genomes have made identification of these elements difficult. Information regarding sequence conservation, exon annotation and repetitive regions can be used to reduce the size of the search region. However, previously developed resources are inadequate for consolidating such information. CONVIRT is a web resource for the identification of transcription factor binding sites and also features comparative genomics. Genomic information on ortholog-independent conserved regions, exons, repeats and sequences is integrated into the virtual chromosome, and statistically over-represented single or combinations of transcription factor binding sites are sought. CONVIRT provides regulatory network analysis for several organisms with long promoter regions and permits inter-species genome alignments. CONVIRT is freely available at http://biosoft.kaist.ac.kr/convirt.

• Molecular & Cellular Toxicology
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• v.4 no.1
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• pp.72-77
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• 2008

The present study was undertaken to determine whether pioglitazone treatment influences on the agonist-induced vascular smooth muscle contraction and, if so, to investigate the related mechanism. The measurement of isometric contractions using a computerized data acquisition system was combined with molecular experiments. Pioglitazone decreased Rho-kinase activating agonist-induced contraction but not phorbol ester-induced contraction suggesting the least involvement of $Ca^{2+}$-independent thin filament regulation of contractility. Furthermore, pioglitazone decreased thromboxane $A_2$ mimeticinduced phosphorylation of MYPT1 at Thr855, the newly-highlighted site, instead of Thr696. In conclusion, this study provides the evidence and possible related mechanism concerning the vasorelaxing effect of pioglitazone as an antihypertensive on the agonist-induced contraction in rat aortic rings regardless of endothelial function.