• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.14-14
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• 2003

Potato virus X (PVX), the type member of Potexvirus genus, is a flexuous rod-shaped virus containing a single-stranded (＋) RNA. Infection by PVX produces genomic plus- and minus-strand RNAs and two major subgenomic RNAs (sgRNAs). To understand the mechanism for PVX replication, we are studying the cis- and/or trans-acting elements required for RNA replication. Previous studies have shown that the conserved sequences located upstream of two major sgRNAs, as well as elements in the 5' non-translated region (NTR) affect accumulation of genomic and sg RNAs. Complementarity between sequences at the 5' NTR and those located upstream of two major sgRNAs and the binding of host protein(s) to the 5' NTR have shown to be important for PVX RNA replication. The 5 NTR of PVX contains single-stranded AC-rich sequence and stem-loop structure. The potential role(s) of these cis-elements on virus replication, assembly, and their interaction with viral and host protein(s) during virus infection will be discussed based on the data obtained by in vitro binding, in vitro assembly, gel shift mobility assay, host gene expression profiling using various mutants at these regions.

• Journal of Plant Biology
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• 제30권1호
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• pp.69-77
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• 1987

From the single cell cultures of haploid tobacco (Nicotiana tabacum L. cv. NC 2326) glyphosate-resistant plants were regenerated. After treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and then inoculated onto the LS medium supplemented with 1 mM glyphosate, the single cells survived formed colonies and calluses in 65 and 95 days after culture, respectively, and then whole plants were regenerated in 0.1 mM glyphosate-containing medium from the selected calluses. There was no difference in fresh weight and shikimate content between the selected and normal haploid calluses. When sprayed with 0.1 mM glyphosate, the shikimate contents in the regenerated and normal plants were 0.659 and 20.816 mol/g fr. wt., while that in other normal plants which were not sprayed was 0.921. In addition, the calluses induced from the regenerated plants grew without showing any retardation when treated with glyphosate. These results indicate that the secelcted calluses and regenerated plants are resistant to glyphosate.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.9-14
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• 2006

As an alternative method to produce low cost reagents for immunodiagnosis and protect the plants from viral disease, a gene encoding a single chain variable fragment(scFv) recombinant antibody targeted to the coat protein of cucumber mosaic virus (CMV) was expressed in Nacotiana tabacum. The source of the scFv recombinant antibody gene was from spleen tissue of an immunized mouse. The gene was initially cloned into the pCANTAB5E phagemid and expressed in E. coli. In the following study, the antibody gene was subcloned into the plant expression vector, pCAMBIA-1301 and introduced into tobacco leaf tissue via Agrobacterium tumefacients mediated transformation. After transformation, 56 out of 58 plants were shown to carry the desired anti-CMV scFv gene by PCR analysis. Overall, only 12.5% of the 56 putative transgenic plants were found to express the antibody to a detectable level.

• 대한기계학회논문집A
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• 제20권2호
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• pp.399-407
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• 1996

A fuzzy learning algorithm to get the optimal fuzzy rules is presented in this paper. The algorithm introduces a reference model to generate a desired output and a performance index funtion instead of the performance index table. The performance index funtion is a cost function based on the error and error-rate between the reference and plant output. The cost function is minimized by a gradient method and the control input is also updated. In this case, the control rules which generate the desired response can be obtained by changing the portion of the error-rate in the cost funtion. In SISO(Single-Input Single- Output)plant, only by the learning delay, it is possible to experss the plant model and to get the desired control rules. In the long run, this algorithm gives us the good control rules with a minimal amount of prior informaiton about the environment.

• 전기학회논문지
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• 제62권5호
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• pp.604-611
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• 2013

This paper presents an second harmonic elimination method based on the impedance variation scheme. Through the power quality analysis from the nonlinear loads connected on the power system, the second harmonic problems have been analyzed with a case study analysis and the experiments. In the paper, the second Harmonic generation was simulated with a single phase SCR rectifier and the analytical model is proposed for the second harmonic generation. A novel impedance variation scheme is proposed and analyzed to eliminate the second harmonic. The experiment has been performed on the 60(MVA) industry manufacturing plant. The experimental result demonstrates the proposed impedance variation scheme successfully operate on the 60(MVA) industry manufacturing plant.

• The Plant Pathology Journal
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• 제22권2호
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• pp.164-167
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• 2006

An immunocapture reverse transcription-polymerase chain reaction (IC/RT-PCR) was developed for simultaneous detection of two pepper-infecting RNA viruses, Pepper mud mottle virus (PMMoV) and Tobacco mild green mosaic virus (TMGMV). The assay could be performed in a single tube for simultaneous and sensitive detection of these tobamoviruses. This detection system revealed thousand-fold increase in detection sensitivity compare to ELISA. This method could save time and reagent cost compare to common RT-PCR which needs several reactions and several procedures of viral RNA extractions for the same number of samples.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.485-485
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• 2000

A design method of model following control system using neural networks is proposed. An unknown nonlinear single-input single-output plant is identified using a multilayer neural networks. A linear controller is designed fer the linear approximation model obtained by linearinzing the identification model. The identification model is also used as a plant emulator to obtain the prediction error. Deficient servo performance due to controlling nonlinear plant with only linear controller is mended by adjusting the linear controller output using the prediction output and the parameters of the identification model. An optimal preview controller is adopted as the linear controller by reason of having good servo performance lowering the peak of control input. Validity of proposed method is illustrated through a numerical simulation.

• Mycobiology
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• 제34권1호
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• pp.34-37
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• 2006

Growth responses of Zea mays and Glycine max to colonization by mixture of combination of three species of arbuscular mycorrhizal (AM) fungi, two species of Glomus and a species of Scutellospora were compared. In Zea mays, plants inoculated with single species of AM fungi showed significantly higher in dry weight than non-mycorrhizal plant for all three AM fungal species. Also, growth of plants inoculated with spores of two species of AM fungi was significantly higher than nonmycorrhizal control except for plants inoculated with two Glomus species. When three species of AM fungi were inoculated, the plants showed the highest growth. In Glycine max, plants with single AM fungal species inoculation were not significantly different in plant growth from nonmycorrhizal plants. When the plants were inoculated with combination of two or more AM fungal species, their growth significantly increased compared to nonmycorrhizal plants. In both plant species, mycorrhizal root colonization by Scutellospora species was significantly lower than by Glomus species.

• 대한전기학회논문지
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• 제32권4호
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• pp.136-144
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• 1983

In this paper, a new scheme of implicit MRAC is presented for single input single output discrete system. The MRAC can be applied to the nonminimum phase system, too. They have simple structure because the parameters of the controller are estimated directly by changing the plant output equation properly. In this scheme, the observation process is well seperated from the adaptation process, so the adaptation algorithm is derived from the exponentially weighted least square method which has fast convergence characteristics and can deal with the time varying plant. The consistency of the estimated parameter is proved. And it is also proved the whole system has the stabilizing property. The effectiveness of the algorithm and the structure is illustrated by the computer simulation of the model reference adaptive control for a third order plant. It is proposed how to select the selectable parameters in the adaptive control system from the simulation results.

• Mycobiology
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• 제32권4호
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• pp.197-198
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• 2004

A new technique for single spore isolation was developed for predacious fungi forming constricting rings directly on the spores using Dactylaria brochopaga and Arthrobotrys dactyloides. Constricting rings were induced directly on the spores by transferring the spores in 25 ppm solution of DL-Valine in sterile distilled water. Freshly hatched and thoroughly washed second stage juveniles of Meloidogyne incognita were transferred into cavity blocks containing induced rings for trapping and killing of nematodes. The killed nematodes were surface sterilized with streptomycin and inoculated into petri dishes containing maize meal agar media with 100 ppm streptomycin. The petri dishes were incubated at $29{\pm}1^{\circ}C$ for few days which yielded axenic culture of these fungi.