• Journal of the Korean Magnetic Resonance Society
• /
• v.7 no.2
• /
• pp.89-97
• /
• 2003

Foot-and-mouth disease virus (FMDV) belongs to the aphthovirus genus within the picornavirus which has a single copy of a positive sense RNA. The translation initiation process of FMDV occurs by a cap-independent mechanism directed by a highly structured element (∼435 nt) termed an internal ribosome entry site (IRES). We have designed and prepared FMDV 4-2 RNA (28nt) by in vitro transcription. The 2D NMR data revealed that FMDV 4-2 IRES domain RNA has a flexible loop and bulge conformation. In further study, we need to make an isotope labeled RNA sample and conduct 3D NMR experiments to completely determine the 3D structure. This study may establish a new drug design strategy to treat foot-and mouth disease.

• Journal of the Korean Society of Safety
• /
• v.28 no.1
• /
• pp.74-80
• /
• 2013

This study describes structural reliability analysis of actively-controlled structure for which random vibration analysis is incorporated into the first-order reliability method (FORM) framework. The existing approaches perform the reliability analysis based on the RMS response, whereas the proposed study uses the peak response for the reliability analysis. Therefore, the proposed approach provides us a meaningful performance measure of the active control system, i.e., realistic failure probability. In addition, it can deal with the uncertainties in the system parameters as well as the excitations in single-loop reliability analysis, whereas the conventional random vibration analysis requires double-loop reliability analysis; one is for the system parameters and the other is for stochastic excitations. The effectiveness of the proposed approach is demonstrated through a numerical example where the proposed approach shows fast and accurate reliability (or inversely failure probability) assessment results of the dynamical active control system against random seismic excitations in the presence of parametric uncertainties of the dynamical structural system.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.21 no.11
• /
• pp.2009-2014
• /
• 2017

A novel structure of phase locked loop (PLL) with a time constant comparator and a current compensator has been proposed. The proposed PLL uses small capacitors which are impossible for stable operation in a conventional PLL. It is small enough to be integrated into a single chip. The time constant comparator detects the loop filter output voltage variations using signals which are passed through small and large RC time constants. The signal from the large RC time constant node is the average of the loop filter output voltage. The output voltage of another node is approximately equal to the present loop filter voltage. The output of the time constant comparator controls a current compensator and charge/discharge small size loop filter capacitors. It makes the proposed PLL operate stably. It has been simulated and proved by HSPICE in a CMOS $0.18{\mu}m$ 1.8V process.

• Journal of Microbiology
• /
• v.42 no.3
• /
• pp.194-199
• /
• 2004

Investigation of the expression of the riboflavin (rib) genes, which are found immediately downstream of luxG in the lux operon in Photobacterium phosphoreum, provides more information relevant to the evolution of bioluminescence, as well as to the regulation of supply of flavin substrate for bacterial bioluminescence reactions. In order to answer the question of whether or not the transcriptions of lux and rib genes are integrated, a transcriptional termination assay was performed with P. phoxphoreum DNA, containing the possible stem-loop structures, located in the intergenic region of luxF and luxE ($\Omega$$\_$A/), of luxG and ribE ($\Omega$$\_$B/), and downstream of ribA ($\Omega$$\_$c/). The expression of the CAT (Chloram-phenicol Acetyl Transferase) reporter gene was remarkably decreased upon the insertion of the stem-loop structure ($\Omega$$\_$c/) into the strong lux promoter and the reporter gene. However, the insertion of the structure ($\Omega$$\_$B/) into the intergenic region of the lux and the rib genes caused no significant change in expression from the CAT gene. In addition, the single stranded DNA in the same region was protected by the P. phosphoreum mRNA from the Sl nuclease protection assay. These results suggest that lux genes and rib genes are part of the same operon in P. phosphoreum.

• Journal of Power Electronics
• /
• v.19 no.1
• /
• pp.254-264
• /
• 2019

This paper proposes an indirect current control technique based on a proportional resonant (PR) approach for the seamless mode transfer of utility interactive inverters. Direct-current and voltage hybrid control methods have been used for inverter control under grid-connected and islanded modes. A large bandwidth can be selected due to the structure of single-loop control. However, this results in poor dynamic transients due to sudden changes of the controller during mode changes. Therefore, inverter control based on indirect current is proposed to improve the dynamic transients by consistently controlling the output voltage under all of the operation modes. A PR-based indirect current control topology is used in this study to maintain the load voltage quality under all of the modes. The design processes of the PR-based triple loop are analyzed in detail while considering the system stability and dynamic transients. The mode transfer techniques are described in detail for both sudden unintentional islanding and islanded mode voltage quality improvements. In addition, they are described using the proposed indirect control structure. The proposed method is verified by the PSiM simulations and laboratory-scale VDER-HILS experiments.

• Journal of Institute of Control, Robotics and Systems
• /
• v.3 no.2
• /
• pp.187-196
• /
• 1997

This paper presents a semi-automatic methodology to synthesize executable digital controller saftware in a multi-loop control system. A digital controller is described by a task graph and end-to-end timing requirements. A task graph denotes the software structure of the controller, and the end-to-end requirements establish timing relationships between external inputs and outputs. Our approach translates the end-to-end requirements into a set of task attributes such as task periods and deadlines using nonlinear optimization techniques. Such attributes are essential for control engineers to implement control programs and schedule them in a control system with limited resources. In current engineering practice, human programmers manually derive those attributes in an ad hoc manner: they often resort to radical over-sampling to safely guarantee the given timing requirements, and thus render the resultant system poorly utilized. After task-specific attributes are derived, the tasks are scheduled on a single CPU and the compiled kernel is synthesized. We illustrate this process with a non-trivial servo motor control system.

• Journal of Power Electronics
• /
• v.13 no.5
• /
• pp.884-895
• /
• 2013

In this paper, the Fourier-based PLL (Phase-locked Loop) is introduced with a new structure, capable of selective harmonic detection in single and three-phase systems. The application of the FB-PLL to harmonic detection is discussed and a new model applicable to three-phase systems is introduced. An analysis of the convergence of the FB-PLL based on a linear model is presented. Simulation and experimental results are included for performance analysis and to support the theoretical development. The decomposition of an input signal in its harmonic components using the Fourier theory is based on previous knowledge of the signal fundamental frequency, which cannot be easily implemented with input signals with varying frequencies or subjected to phase-angle jumps. In this scenario, the main contribution of this paper is the association of a phase-locked loop system, with a harmonic decomposition and reconstruction method, based on the well-established Fourier theory, to allow for the tracking of the fundamental component and desired harmonics from distorted input signals with a varying frequency, amplitude and phase-angle. The application of the proposed technique in three-phase systems is supported by results obtained under unbalanced and voltage sag conditions.

• Journal of Power Electronics
• /
• v.15 no.5
• /
• pp.1409-1420
• /
• 2015

In this paper, an algorithm using two bi-quad filters to suppress the wide-band resonance for PMSM servo systems is proposed. This algorithm is based on the double bi-quad filters structure, so it is named, "double bi-quad filter." The conventional single bi-quad filter method cannot suppress unexpected mechanical terms, which may lead to oscillations on the load side. A double bi-quad filter structure, which can cancel the effects of compliant coupling and suppress wide-band resonance, is realized by inserting a virtual filter after the motor speed output. In practical implementation, the proposed control structure is composed of two bi-quad filters on both the forward and feedback paths of the speed control loop. Both of them collectively complete the wide-band resonance suppression, and the filter on the feedback path can solve the oscillation on the load side. Meanwhile, with this approach, in certain cases, the servo system can be more robust than with the single bi-quad filter method. A step by step design procedure is provided for the proposed algorithm. Finally, its advantages are verified by theoretical analysis and experimental results.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.15 no.12 s.105
• /
• pp.1370-1377
• /
• 2005

The bottom structure of an instrumented capsule is a part which is joined at the receptacle of the flow tube in the reactor in-core. A geometrical change of the bottom structure has an effect on the pressure drop and the vibration of the capsule. The out-pile test to evaluate the structural integrity of the material capsule called 04M-17U was performed by using a single channel and a half core test loop. From the pressure drop test, the optimized diameter of the cone shape's bottom structure which satisfies HANARO's flow requirement (19.6 kg/s) is 71 mm. The maximum displacement of the capsule measured at the half core test loop is lower than 1.0 mm. From the analysis results, it is found that the test hole will not be interfered with near the flow tubes because its displacement due to the cooling water is very small at 0.072 mm. The fundamental frequency of the capsule under water is 9.64 Hz. It is expected that the resonance between the capsule and the fluid flow due to the cooling water in HANARO's in-core will not occur. Also, the new bottom structure of a solid cone shape with 71 mm in diameter will be applicable to the material and special capsules in the future.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2005.11a
• /
• pp.782-787
• /
• 2005

The bottom structure of an instrumented capsule is a part which is joined at the receptacle of the flow tube in the reactor in-core. A geometrical change or the bottom structure has an effect on the pressure drop and the vibration of the capsule. The out-pile test to evaluate the structural Integrity of the material capsule called 04M-l7U was performed by using a single channel and a half core test loop. From the pressure drop test, the optimized diameter of the cone shape's bottom structure which satisfies HANARO's flow requirement (19 6 kg/s) is 71 mm. The maximum displacement of the capsule measured at the half core test loop is lower than 1.0 mm. From the analysis results, it is found that the test hole will not be interfered with near the flow tubes because its displacement due to the cooling water is very small at 0.072 mm. The fundamental frequency of the capsule under water is 9.64 Hz. It is expected that the resonance between the capsule and the fluid flow due to the cooling water in HANARO's In-core will not occur. Also, the new bottom structure of a solid cone shape with 71 mm in diameter will be applicable to the material and special capsules in the future.