• Interdisciplinary Bio Central
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• v.4 no.2
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• pp.5.1-5.9
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• 2012

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

• Journal of Information Technology Services
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• v.3 no.1
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• pp.63-77
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• 2004

The purpose of this research is to identify what factors influence a customer's purchasing intention and to identify if customer's attitudes are different between an Internet shopping mall in Singapore and Korea. To perform a cross-nation analysis between Singapore and Korea, this research reapplied the finding of Liao and Cheung(2001) in a Singapore Internet shopping mall, including transactions security, Internet usage, price, shopping experience, vendor quality, network speed. The empirical results of this research indicate that all hypotheses in our model are significant; therefore, Internet usage, price, vendor quality and network speed influence customer's purchasing intention positively and transactions security and shopping experience negatively. The results of a cross-nation analysis indicate that customer's attitudes in both Singapore and Korea are the same. Therefore, Asia's with similar cultures are the same factors In Internet shopping mall. In Conclusion, with the rapid growth of the Internet market in Asia, many cross-nations researches based on Asia culture must be published. Therefore, this research will be the foundation of these researches.

• Archives of Plastic Surgery
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• v.40 no.5
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• pp.584-588
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• 2013

Background The ability to achieve a long-term, stricture-free urethral repair is one of the ongoing challenges of reconstructive urologic surgery. A successful initial repair is critical, as repeat procedures are difficult, owing to distortion, scarring, and short urethral stumps. Methods We describe a technique in which the gracilis muscle flap is laid on or wrapped around the urethral repair site to provide a well-vascularised soft tissue reinforcement for urethral repair. This technique promotes vascular induction, whereby a new blood supply is introduced to the repair site to improve the outcome of urethral repair or anastomotic urethroplasty. The surface contact between the muscle flap and the repair site is enhanced by the use of fibrin glue to improve adherence and promote inosculation and healing. We employed this technique in 4 patients with different urethral defects. Results After a follow-up period of 32 to 108 months, all of the urethral repairs were successful without complications. Conclusions Our results suggest that the use of a gracilis muscle flap to vascularise urethral repairs can improve the outcome of challenging urethral repairs.

• BMB Reports
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• v.50 no.5
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• pp.247-256
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• 2017

CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research.

• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.8.1-8.1
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• 2007

• Safety and Health at Work
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• v.12 no.1
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• pp.133-135
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• 2021

The COVID-19 pandemic has resulted in movement restrictions being instituted globally and the cessation of work at many workplaces. However, during this period, essential services such as healthcare, law enforcement, and critical production and supply chain operations have been required to continue to function. In Singapore, measures were put in place to protect the workers from infection at the workplace, as well as to preserve the operational capability of the essential service in a COVID-19 pandemic environment. This paper critically analyses the measures that were implemented and discusses the extension to broader general industry.

• SUVANNABHUMI
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• v.10 no.2
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• pp.143-153
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• 2018

The paper comments on the contribution of Oscar Salemink on his personal intellectual journal from Vietnam to Europe and back again. This then leads to the contemplation of the construction of Southeast Asia as a "place" or "locality", early preoccupations within the region of the national dimension. And more recent developments in universities in Singapore, examining the continuing perceptions of Southeast Asia as a region and Singapore as its "gateway", and the increasing interest in "connectivities" and transnational relations between the region and other parts of Asia and the wider world.

• Clinics in Shoulder and Elbow
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• v.27 no.1
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• pp.79-87
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• 2024

Rotator cuff disease is a common cause of shoulder pain for which partial-thickness rotator cuff tears occupy a significant proportion. Such tears are often difficult to diagnose and manage in the general clinic setting. A review of the available literature from well-known databases was performed in this study to provide a concise overview of partial-thickness rotator cuff tears to aid physicians in their understanding and management.

• Steel and Composite Structures
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• v.3 no.4
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• pp.289-306
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• 2003

A series of tests was conducted to study the behaviour of steel-composite sandwich beams under low velocity hard impact. Damage characteristic and performance of sandwich beams with different spacing of shear connector were evaluated under impact loading. Thin steel plates were used as top and bottom skins of the sandwich beams and plain concrete was used as the core material. Shear connectors were provided by welding of angle sections on steel plates. The sandwich beams were impacted at their midpoint by a hemi-spherical nose shaped projectile dropped from various heights. Strains on steel plates were measured to study the effects of impact velocity or impact momentum on the performance of sandwich beams. Spacing of shear connectors is found to have significant effects on the impact response of the beams.

• East Asian mathematical journal
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• v.33 no.2
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• pp.235-255
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• 2017

This study focuses on the teaching of fraction related to curriculum, introducing time of fraction, the meaning of fractions in textbook, material of teaching of fraction concept, teaching model of introducing time of fraction concept, special cases of teaching fraction and common points of representation of fraction among Korea, New Zealand and Singapore. For this study, Korea's mathematics textbooks(3-1, 3-2, 4-1, 5-1, 6-1) and New National Curriculum Mathematics(3, 4, 5. 6. 7)of New Zealand and New Syllabus Primary Mathematics(2B, 3B, 4A, 4B, 5A, 6A)of Singapore were selected for comparison and analysis. As a results we will suggest a reference to the development of mathematical curriculum, teaching fraction and improving the quality of the textbook through a method of comparative analysis of Korea, New Zealand and Singapore.