• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.6
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• pp.109-123
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• 2020

The purpose of this study is to provide basic data for the establishment of future countermeasures by diagnosing the growth status and actual condition of undesignated old trees distributed in Hyeongok-myeon, Gyeongju, Gyeongsangbuk-do. The number of old trees surveyed was 2 weeks in Gajeong-ri, 2 weeks in Haguri, 3 weeks in Nae-Tae-ri, 1 week in Nawon-ri, 6 weeks in Oryu-ri, 3 weeks in Sangguri, and 2 weeks in Sohyeon-ri, The trees species composition was 6 trees Celtis sinensis Pers., 1 Diospyros lotus L. trees, 4 trees Salix chaenomeloides Kimura trees, 2 Styphnolobium japonicum L. trees, and 7 Zelkova serrata (Thunb.) Makino trees. Growth status is 7.1~22.0m in height, 14.6~25.1m in long axis, 10.2~19.2m in short axis, root diameter is 76.0~236.4cm, diamter at breast height is 67.0~220.0cm, soil acidity is pH4.9~7.0, soil The hardness was measured to be 4.0-27.0mm. The result grade of the scoring data of health information is represented by monitoring generally, monitoring critically, and absolute monitoring, and it was confirmed that out of the 20 trees population in Hyeongok-myeon, the general monitoring grade was 7 weeks, the major monitoring grade was 13 weeks, and there was no absolute monitoring grade. Accordingly, the number of old trees of the general surveillance level was maintained at the current level, and ecological surgical operations were introduced for the major surveillance level, but the case of village forests should be different, and sequential treatments were the old tree urgently needed. The level and bark of the target tree, the state of the crown, the root exposure, the presence of pests and pests, the vitality and the ground condition, the degree of pollution are normal, poor, or very poor, operation and protection management, soil improvement, removal of cover, and disinfection were urgently needed for the old trees with the surveyed data. In order to compensate for these matters, continuous monitoring and management measures for the old number should be sought.

• Journal of Korean Society of Forest Science
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• v.111 no.4
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• pp.490-501
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• 2022

In this study, the isoprene and terpene emissions from 32 major urban tree species were investigated. We conducted sampling using a dynamic enclosure system between June and July 2021. Seedlings aged < three years were enclosed in a chamber consisting of a 400 L transparent Tedlar bag. The air flow from the outlet of the chamber was sampled using Tenax-filled sorbent tubes under standard conditions (temperature: 30°C; PAR: 1,000 μmol/m²/sec). A thermal desorption gas chromatography/mass spectrometry system was used to analyze the following 38 biogenic volatile organic compounds: isoprene, monoterpenes, sesquiterpenes, oxygenated monoterpenes, and oxygenated sesquiterpenes. Isoprene emitters included Quercus mongolica, Salix koreensis, Robinia pseudoacacia, and Salix chaenomeloides. Monoterpene emitters included Pinus strobus, Cedrela sinensis, and Cercis chinensis. The monoterpene emission profiles were dominated by á-pinene, myrcene, camphene, and limonene. The predominant oxygenated monoterpene and oxygenated sesquiterpene were eucalyptol and caryophyllene oxide, respectively. For all species, the contributions of sesquiterpenes and oxygenated sesquiterpenes were relatively low.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.5
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• pp.187-192
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• 2022

The purpose of this study is to examine the effect of herbal medicine complex (HMC) containing Camellia sinensis L., Duchoesna chrysantha, Houttuynia cordata Thunberg, Poncirus trifoliata Rafinesque on skin inflammation and atopic dermatitis. First, we examined the anti-inflammatory effect of HMC in TNF-α induced human keratinocytes (HaCaT cell). Real-time PCR and western blotting were performed to evaluate the expression of inflammatory cytokines (e.g., iNOS, COX-2, IL-6, IL-8) mRNA and protein. Four-weeks old male NC/Nga mice were treated with 1% 2,4-dinitrochlorobenzene (DNCB) solution and used as an atopic dermatitis mice model. And, HMC (200 mg/kg or 400 mg/kg) was administered directly into the stomach of mice for 4 weeks, and blood or serum analysis, tissue staining were performed after oral gavage. As a result HMC inhibited the mRNA expression of iNOS, COX-2, IL-6, and IL-8, which had been increased by TNF-α in HaCaT cells. In addition, the protein expression was also significantly suppressed in the same way as the mRNA expression results. The in vivo experiment results showed that, HMC administration reduced thickening of the epidermis and infiltration of eosinophil into the skin stratum basale compared to DNCB treatment. In addition, HMC administration significantly reduced the inflammatory cytokines (IL-4, IL-5, IL-6, and IL-13) production and immunocyte (white blood cell, lymphocyte, neutrophil, and eosinophil) count compared to DNCB treatment. Moreover, the serum IgE and histamine level was decreased by HMC administration. These results suggest that HMC can be used as effective herbal medicine extract for skin inflammation and atopic dermatitis. And this study may contribute to the development of the herbal medicine-based drug for the treatment of skin inflammation and atopic dermatitis.

• Journal of Korean Society of Forest Science
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• v.112 no.1
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• pp.11-22
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• 2023

This study was established to provide basic information necessary for ecological management to restore the naturalness of black locust (Robinia pseudoacacia L.) plantations located in the mountains of Gyeongbuk, Korea. Using vegetation data collected from 200 black locust stands, vegetation types were classified using the TWINSPAN method, the spatial arrangement status according to the environmental gradient was identified through DCA analysis, and a synoptic table of communities was prepared based on the diagnostic species determined by determining community fidelity (Φ) for each vegetation type. The vegetation types were classified into seven types, namely, Quercus mongolica-Polygonatum odoratum var. pluriflorum type, Castanea crenata-Smilax china type, Clematis apiifolia-Lonicera japonica type, Rosa multiflora-Artemisia indica type, Quercus variabilis-Lindera glauca type, Ulmus parvifolia-Celtis sinensis type, and Prunus padus-Celastrus flagellaris type. These types usually reflected differences in complex factors such as altitude, moisture regime, successional stage, and disturbance regime. The mean relative importance value of the constituent species was highest for black locust(39.7), but oaks such as Quercus variabilis, Q. serrata, Q. mongolica, Q. acutissima, and Q. aliena were also identified as important constituent species with high relative importance values, indicating their potential for successional trends. In addition, the total percent cover of constituent species by vegetation type, life form composition, species diversity index, and indicator species were compared.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.1007-1016
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• 2023

In this study, the effect of functional substances such as polyphenols and flavonoids contained in large quantities in red ginseng and herbal medicines on the antioxidant activity properties and flavor effect were investigated by increasing the content and activity of functional ingredients by convert red ginseng through a steaming heat-drying process compared to the traditional dry processing method of ginseng and herbal medicines. According to the experimental results, the addition of pre-heat treatment significantly increased antioxidant properties such as DPPH radical scavenging ability, polyphenols, and flavonoids. In addition, during the steaming and heat-dried red ginseng manufacturing process, the amino-carbonyl browning reaction was promoted, resulting in increased brownness and a savory flavor. However, the content of ginsenosides, the main medicinal ingredient in red ginseng, became the main cause of the bitter and harsh taste. In addition, the bitter and harsh taste of red ginseng has been significantly improved by roasting and producing powder, but on the other hand, Radix Angelicae sinensis, polymorphic angelica and Peony, which are used as main medicinal ingredients in oriental medicine for nutritional tonic prescriptions such as 10 herbal medicine and 4 herbal medicine have a very strong herbal medicine-specific flavor and have a bitter and harsh taste. It is so strong that in order to use it as an instant extraction material, it was reviewed that a steaming and heating manufacturing method was needed during the manufacturing process.

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.270-277
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• 2023

Since its introduction in 1973, the bass, Micropterus salmoides, has spread to dams and rivers in Korea, adversely affecting native fish communities. In this study, after complete removal of bass from Bukchosan Reservoir, an farm reservoir that has lost its function, an attempt was made to indirectly confirm the adverse effects of bass on fish by comparing fish communities in reservoirs of a similar size where bass inhabit. The study was conducted three times a year from 2019 to 2022. As a result of the study, in Bukchosan Reservoir, where the species composition of fish was judged to be simplified due to the habitat of bass, only four species such as Carassius auratus and Rhinogobius brunneus that could be used as a food source for bass appeared before the removal of bass, but after the removal of bass. The appearance and increase of the population of the introduced small freshwater fish (Oryzias sinensis, Rhinogobius giurinus) were confirmed. When comparing Gangjeong Reservoir, Susim Reservoir, which are other reservoirs inhabited by bass investigated in this study, and Bukchosan Reservoir, this result is judged to show a trend of stabilization and recovery of fish species after removal of bass in small reservoirs with high predation pressure of bass.

• Food Science and Preservation
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• v.30 no.2
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• pp.235-246
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• 2023

Yellowball (Citrus hybrid cv. Yellowball ) is a new citrus hybrid between Haruka (C. tamurana × natsudaidai ) and Kiyomi (C. unshiu × sinensis) and is known to possess strong antioxidant activity. However, detailed information on the antioxidant components of its peel has not yet been reported. This study evaluated the antioxidant activity of the peel and identified the antioxidant components by fractionating a methanolic extract of Yellowball peels using liquid-liquid extraction with n-hexane, ethyl ether (ether), ethyl acetate (EA), butanol, and water. The phenolic contents and antioxidant activities of the n-hexane, ether, and EA fractions were higher than those of the other fractions, and these fractions were further separated by semi-preparative high-performance liquid chromatography (HPLC). Four antioxidant peaks, EA1, EA2, EA3, and He1, were isolated and analyzed using ultra-performance liquid chromatography-quadrupole-time- of-flight mass spectrometry (UPLC-Q-TOF MS). Sinapoyl glucoside and hesperidin were identified in EA2 and EA3, respectively, and a polymethoxylated flavone (PMF) complex (5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, natsudaidain, tetrameth- oxyflavone, and tangeretin) was identified in He1. A compound in EA1 with m/z 223.0246 [M-H] could not be identified and was named unknown2. The antioxidant activity of unknown2 (IC₅₀=69.17 ㎍/mL) was similar to that of Trolox, which was noted as a major antioxidant in Yellowball peel. Further studies on the antioxidant capacity of Yellowball peel are required; however, these results provide a foundation for using Yellowball peel as an antioxidant.

• Journal of Food Hygiene and Safety
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• v.39 no.3
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• pp.209-220
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• 2024

Cavier is graded as Beluga, Osetra, and Sevruga based on the species of sturgeon (Acipenser sinensis). In this study, we developed an analytical method for determining the grade of black caviar using DNA barcodes and KASP markers. To identify the sturgeon species, ten black caviar samples were collected, and a reference DNA barcode library was developed using five genes (namely, 16S ribosomal RNA, cytochrome b, cytochrome c oxidase subunit I, cytochrome c oxidase subunit II, and NADH dehydrogenase subunit 5 genes). To develop the KASP markers, we selected 11 markers that could distinguish between the five grades of black caviar. As a result, specific markers for each of the targeted caviars were clustered into FAM-positive sections. DNA barcoding and the KASP assay revealed that one Beluga, six Osetra, and three Sevruga were identified among the ten caviar samples. Moreover, we found that the sturgeon species were mislabeled in two products. Here, we aimed to develop a KASP assay based on SNP that allows rapid and easy identification of caviar grade. These methods are expected to contribute to preventing the distribution of illegal products.

• Journal of Bio-Environment Control
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• v.33 no.1
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• pp.1-11
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• 2024

In this study, five artificial intelligence (AI) models: Inception v3, SqueezeNet (local), VGG-16, Painters, and DeepLoc were used to classify tea leaf diseases. Eight image categories were used: healthy, algal leaf spot, anthracnose, bird's eye spot, brown blight, gray blight, red leaf spot, and white spot. Software used in this study was Orange 3 which functions as a Python library for visual programming, that operates through an interface that generates workflows to visually manipulate and analyze the data. The precision of each AI model was recorded to select the ideal AI model. All models were trained using the Adam solver, rectified linear unit activation function, 100 neurons in the hidden layers, 200 maximum number of iterations in the neural network, and 0.0001 regularizations. To extend the functionality of Orange 3, new add-ons can be installed and, this study image analytics add-on was newly added which is required for image analysis. For the training model, the import image, image embedding, neural network, test and score, and confusion matrix widgets were used, whereas the import images, image embedding, predictions, and image viewer widgets were used for the prediction. Precisions of the neural networks of the five AI models (Inception v3, SqueezeNet (local), VGG-16, Painters, and DeepLoc) were 0.807, 0.901, 0.780, 0.800, and 0.771, respectively. Finally, the SqueezeNet (local) model was selected as the optimal AI model for the detection of tea diseases using tea leaf images owing to its high precision and good performance throughout the confusion matrix.

• Journal of Korean Society of Forest Science
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• v.113 no.3
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• pp.327-338
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• 2024

Forest road construction can degrade the physical and biological environments of forest ecosystems. Although this degradation may be temporary, some research has shown the potential for the long-term recovery of the original ecosystem. This study investigated changes in communities' structures over time to understand the process of ecosystem change following road construction. Data were collected from 63 plots, each measuring 25m², in Buyeo-gun, Chungcheongnam-do, including plots from roads constructed in 1998 (25 years elapsed), 2021 (two years elapsed), and 2022 (one year elapsed), using phytosociological methods. The results showed that the importance of the values of Pinus densiflora an d Quercus variabilis in the tree and subtree layers of the 25-year-old cut slopes were similar to those of the control plots, indicating the significant recovery of the original ecosystem's structure and function after 25 years. Species diversity analysis revealed the higher evenness and lower dominance of the cut slopes and road surfaces attributed to the high dominance of species such as Arundinella hirta and Miscanthus sinensis. The community similarity index and detrended correspondence analysis (DCA) indicated that the control plots, all the edge plots, and the 25-year-old cut slopes could be considered part of the same community. In conclusion, forest roads in place for 25 years appear to have been restored to the level of the original ecosystem. These findings can serve as valuable ecological data for understanding the vegetation recovery process at future forest road construction sites.