• The Korean Journal of Ecology
• /
• 제21권5_1호
• /
• pp.457.1-463
• /
• 1998

the research has been made for alkali and metal element concentrations in top soils and plants from the abandoned coal mine, Keumsan, Chungnam Province. Samples of the top soil and plant (Miscanthus sinensis and Pinus rigida) were collected from the mine area in which was divided into t재 regions the polluted region influenced by the coal mining and the non-polluted region. pH of the top soils was 3.16-4.33 in the polluted region. Ca, Sr and P concentrations were high in the polluted soils, and Al and Ba concentrations were high in the non-polluted soils. No differences were found in K, Na and Ti concentrations. M. sinensis was higher in the element concentrations than P. rigida. In the average concentration of the alkali and metal element, M. sinensis showed high Cs and Na in the polluted region, and high Ba, Ca, K, Sr and concentrations in the non-polluted region. P. rigida had high Cs, Na and Rb concentrations in the polluted region. M. sinensis and P. rigida were higher in the root than above-ground part in the most element, but Ca and K. Ca, K and Na concentrations within both plants had higher than those of soils.

• 생약학회지
• /
• 제42권3호
• /
• pp.223-228
• /
• 2011

Chaenomeles sinensis Koehne (Rosaceae) is a deciduous tree and is distributed in China, Korea and Japan. In previous studies on the fruits of C. sinensis, some triterpenoid compouds such as oleanolic acid, tormentic acid were reported. In an ongoing investigation into biologically active compounds from natural products, the methanol extract of the stems of C. sinensis was investigated. By means of the repeated column chromatography using silica gel, Sephadex LH-20, LiChroprep RP-18, betulin (1), tormentic acid (2), 1-${\beta}$-D-glucopyranosyl-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-${\alpha}$-L-rhamnopyranoside (4) were isolated. The chemical structures of compounds 1-4 were determined by the basis of physico-chemical properties and spectroscopic methods such as 1D and 2D NMR. For the isolated compounds (1-4), the inhibitory activity of IL-6 production in TNF-${\alpha}$ stimulated MG-63 cell was examined. Among the isolates, betulin (1), 1-${\beta}$-D-glucopyranosyl-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-${\alpha}$-L-rhamnopyranoside (4) showed inhibitory effects on IL-6 production in TNF-${\alpha}$ stimulated MG-63 cell.

• Parasites, Hosts and Diseases
• /
• 제44권2호
• /
• pp.163-166
• /
• 2006

The present study observed the resistance to reinfection with Clonorchis sinensis in various experimental animals including mice, guinea pigs, rabbits, and dogs, as well as rats and hamsters. The resistance rates to reinfection in rats, mice, hamsters, guinea pigs, rabbits, and dogs were 79.7%, 58.0%, -12.6%, 54.8%, 62.6%, and 6.0%, respectively. Worms recovered from reinfected rats and mice were immature, and significantly smaller than those from the primarily infected (P < 0.01), whereas those from other animals were fully matured to adults. These findings indicate that the protective response against reinfection with C. sinensis is prominent in rats and mice, and that they may be a good animal model to investigate the mechanism of resistance to reinfection with C. sinensis.

• Parasites, Hosts and Diseases
• /
• 제54권5호
• /
• pp.679-684
• /
• 2016

Clonorchiasis, caused by direct contact with Clonorchis sinensis worms and their excretory-secretory products (ESPs), is associated with chronic inflammation, malignant changes in bile ducts, and even cholangiocarcinogenesis. Our previous report revealed that intracellular free radicals enzymatically generated by C. sinensis ESPs cause NF-${\kappa}B$-mediated inflammation in human cholangiocarcinoma cells (HuCCT1). Therefore, the present study was conducted to examine the role of upstream Toll-like receptors (TLRs) on the initial host innate immune responses to infection. We found that treatment of HuCCT1 cells with native ESPs induced changes in TLR mRNA levels in a time-dependent manner, concomitant with the generation of free radicals. ESP-mediated free radical generation was markedly attenuated by preincubation of the cells with TLR1-4-neutralizing antibodies, indicating that at least TLR1 through 4 participate in stimulation of the host innate immune responses. These findings indicate that free radicals triggered by ESPs are critically involved in TLR signal transduction. Continuous signaling by this pathway may function in initiating C. sinensis infection-associated inflammation cascades, a detrimental event leading to progression to more severe hepatobiliary diseases.

• 수산해양기술연구
• /
• 제57권3호
• /
• pp.205-213
• /
• 2021

Two specimens of Hemitrygon izuensis (395.8-471.5 mm in disc width), belonging to the family Dasyatidae (Myliobatiformes), were collected from the waters off north-eastern Jeju-do Island in June 2016 and Pohang in August 2019. This species was characterized by having no spots on dorsal side of disc, white ventral tail fold and short preorbital snout length (15.6-18.3% of disc width). This species is similar to H. akajei and H. sinensis reported in Korea, but differs in small denticles on mid-line of dorsal surface of disc (absent in H. izuensis vs. present in H. akajei and H. sinensis), the length of ventral tail fold (28.1-31.0% in H. izuensis vs. 48.0-48.6% in H. akajei vs. 45.0% in H. sinensis), color of ventral tail fold (white in H. izuensis vs. black in H. akajei and H. sinensis) and small tubercles on the posterior part of tail (absent in H. izuensis vs. present in H. akajei and H. sinensis). We suggest a Korean name for H. izuensis as "I-ju-gal-saeg-ga-o-li" following Kim et al. (2019).

• Parasites, Hosts and Diseases
• /
• 제61권4호
• /
• pp.428-438
• /
• 2023

Clonorchis sinensis is commonly found in East Asian countries. Clonorchiasis is prevalent in these countries and can lead to various clinical symptoms. In this study, we used overlap extension polymerase chain reaction (PCR) and the Xenopus laevis oocyte expression system to isolate a cDNA encoding the choline transporter of C. sinensis (CsChT). We subsequently characterized recombinant CsChT. Expression of CsChT in X. laevis oocytes enabled efficient transport of radiolabeled choline, with no detectable uptake of arginine, α-ketoglutarate, p-aminohippurate, taurocholate, and estrone sulfate. Influx and efflux experiments showed that CsChT-mediated choline uptake was time- and sodium-dependent, with no exchange properties. Concentration-dependent analyses of revealed saturable kinetics consistent with the Michaelis-Menten equation, while nonlinear regression analyses revealed a Km value of 8.3 µM and a Vmax of 61.0 pmol/oocyte/h. These findings contribute to widen our understanding of CsChT transport properties and the cascade of choline metabolisms within C. sinensis.

• Archives of Pharmacal Research
• /
• 제21권6호
• /
• pp.744-748
• /
• 1998

Several praziquantel derivatives have been prepared by the acylation of compound 5, and examined on their biological activity in vitro agninst adult clonorchis sinensis collected from rabbits infected with metacercariae which was isolated from Pseudorasbora parva, a second intermediate host, captured in Nakdong river in Korea.

• Natural Product Sciences
• /
• 제6권2호
• /
• pp.79-81
• /
• 2000

Investigation of the fruits of Chaenomeles sinensis (Rosaceae) resulted in the isolation of a minor flavonoid. The structure of flavanone glycoside was determined to be as $2-hydroxynaringenin-7-O-{\beta}-glucoside$ on the basis of FAB-MS and spectral evidence, especially by 2D-NMR $(^1H-^1H\;COSY,\;HMQC,\;HMBC\;and\;NOESY)$.

• Archives of Pharmacal Research
• /
• 제28권1호
• /
• pp.39-43
• /
• 2005

Eight compounds were isolated from the methanolic extract of the twigs of Celtis sinensis through repeated silica gel and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as two triterpenoids, germanicol and epifriedelanol, two amide compounds, trans-N-caffeoyltyramine and cis-N-coumaroyltyramine, two lignan glycoside, pinoresinol glycoside and pinoresinol rutinoside, and two steroids by spectroscopic analysis.

• 약학회지
• /
• 제37권3호
• /
• pp.306-310
• /
• 1993

The leaves of Cedrela sinensis have been used for food at south area in Korea and oriental medicine for treating enteritis, dysentery and itch. Kaempferol, methyl gailate, quercetin, afzelin, quercitrin, isoquercitrin and rutin were isolated from the leaves of this plant and characterized by spectral data. These compounds are reported for the first time from this plant.