• 경영과학
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• 제11권3호
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• pp.13-26
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• 1994

In this paper we studied an integration scheme of some simplex algorithms and some efficient techniques to get the stable solution in linear programming code. And we developed a linear programming package (LPAK) by introducing this scheme and techniques. In LPAK three different algorithms were integrated, which were two primal simplex algorithms using Two phase method and big-M method respectively, and the dual simplex algorithm. LPAK introduces several heuristic techniques in each step of simplex method in order to enhance the stability and efficiency. They were new heuristic methods in structuring initial basis, choosing entering variable, choosing dropping variable and performing reinversion. The experimental results on the NETLIB problems showed that LPAK provided the stable solutions.

• Korean Journal of Mathematics
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• 제27권2호
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• pp.445-463
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• 2019

Let C[0, T] denote the space of continuous real-valued functions on [0, T]. On the space C[0, T], we introduce a Banach algebra of series of functions which are generalized Fourier-Stieltjes transforms of measures of finite variation on the product of simplex and Euclidean space. We evaluate analytic Feynman integrals of the functions in the Banach algebra which play significant roles in the Feynman integration theory and quantum mechanics.

• Mass Spectrometry Letters
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• 제15권2호
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• pp.69 -78
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• 2024

The advancement of high-throughput omics technologies and systems biology is essential for understanding complex biological mechanisms and diseases. The integration of proteomics and metabolomics provides comprehensive insights into cellular functions and disease pathology, driven by developments in mass spectrometry (MS) technologies, including electrospray ionization (ESI). These advancements are crucial for interpreting biological systems effectively. However, integrating these technologies poses challenges. Compared to genomic, proteomics and metabolomics have limitations in throughput, and data integration. This review examines developments in MS equipped electrospray ionization (ESI), and their importance in the effective interpretation of biological mechanisms. The review also discusses developments in sample preparation, such as Simultaneous Metabolite, Protein, Lipid Extraction (SIMPLEX), analytical techniques, and data analysis, highlighting the application of these technologies in the study of cancer or Huntington's disease, underscoring the potential for personalized medicine and diagnostic accuracy. Efforts by the Clinical Proteomic Tumor Analysis Consortium (CPTAC) and integrative data analysis methods such as O2PLS and OnPLS extract statistical similarities between metabolomic and proteomic data. System modeling techniques that mathematically explain and predict system responses are also covered. This practical application also shows significant improvements in cancer research, diagnostic accuracy and therapeutic targeting for diseases like pancreatic ductal adenocarcinoma, non-small cell lung cancer, and Huntington's disease. These approaches enable researchers to develop standardized protocols, and interoperable software and databases, expanding multi-omics research application in clinical practice.

• 한국지능시스템학회논문지
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• 제8권6호
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• pp.58-69
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• 1998

This paper introduces a novel neuro-fuzzy system based on the polynomial fuzzy neural network(PFNN) architecture. The PFNN consists of a set of if-then rules with appropriate membership functions whose parameters are optimized via a hybrid genetic algorithm. A polynomial neural network is employed in the defuzzification scheme to improve output performance and to select appropriate rules. A performance criterion for model selection, based on the Group Method of DAta Handling is defined to overcome the overfitting problem in the modeling procedure. The hybrid genetic optimization method, which combines a genetic algorithm and the Simplex method, is developed to increase performance even if the length of a chromosome is reduced. A novel coding scheme is presented to describe fuzzy systems for a dynamic search rang in th GA. For a performance assessment of the PFNN inference system, three well-known problems are used for comparison with other methods. The results of these comparisons show that the PFNN inference system outperforms the other methods while it exhibits exceptional robustness characteristics.

• 대한미생물학회지
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• 제17권1호
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• pp.7-14
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• 1982

A model of induction of neoplasia by viruses has develpoed from experimental studies in animals and in cultured cells and oncogenic transformation of cells is the result of integration of viral genetic information into the cellular DNA. The evidence for these associations was derived primarily from seroepidemiologic investigation. However, data indicating that the relation between HSV-2 and cervical cancer fits the model derived from experimental animal studies are not yet sufficient to draw conclusion with regard to the etiologic role the virus in the development of the neoplasms. In other hand, the K562 tumor cell is highly susceptible target for natural killer cell lysis by the lymphocytes of human and murine periperal blood. The characteristics of this effector cell type has been investigated. A study on natural killer cell mediated cytotoxicity(NKMC) against $^{51}Cr$-K562 as target cell was studed in HSV-2 infected ICR mouse. We have studied for susceptibility of HSV-2 against mouse embryo fibroblast(MEF) cells and NKMC from HSV-2 infected mouse. The results obtained that the mouse embryo fibroblast cells culture, the number and size of the cells were markedly increased and formed a monolayers relatively rapid, and become complete monolayer sheet around 72 hrs. Duration of cytopathic effect on MEF cells was rapid by serial passing of HSV-2. The morphology of the HSV-2 infected cells appear to be mainly round, ovium, spindle form and some of them was forming large giant cells. The NKMC was decrease in mouse with HSV-2 and comparison between effector/target cells ratio as 25:1 and 50:1 respectively, the NKMC was found to be more significantly decreased than normal control we have concluded that the natural killer cell activity of the viral infected mouse was shown as a suppressed during the HSV-2 infection, day 7th and 14th.

• Tuberculosis and Respiratory Diseases
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• 제45권3호
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• pp.587-595
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• 1998

연구배경: 유전자치료에 이용되는 adenovirus를 기초로 하는 벡타는 상피세포에 강한 친화력이 있고 증식하지 않는 세포에도 유전자의 이입이 가능하며, in vivo에서 다른 백타에 비해 유전자이입 효율이 높은 장점이 있다. 그러나 adenovirus에 이해 전달된 유전자는 세포의 chromosome에 삽입되지 않기 때문에 세포가 분열하면 딸세포에 형질이 전달되지 않을 뿐 아니라 adenovirus가 숙주의 면역반응을 초래하여 이입된 유전자가 제거됨에 따라 전달된 유전자의 발현기간이 짧은 단점이 있다. 포화지방산인 butyrate는 histone의 acetylation을 증가시켜 전달된 유전자의 발현을 증가시킨다고 한다. 저자들은 유전자치료시 butyrate를 병용투여 할 경우 이입한 유전자의 발현이 증가되고, 따라서 유전자치료의 효과를 증가시킬 수 있을 것이라는 가정하에 butyrate가 herpes simplex virus thymidine kinase 유전자를 이용한 유전자치료에 미치는 영향을 조사하였다. 방 법: 악성 중피종세포인 REN세포에 Ad.CMVtk를 이입한 후 세포들을 3군으로 분류하였다. 1 군은 butyrate를 처리하지 않고 7일간 배양하였으며, 2군은 이입 후 1일째에 그리고 3군은 이입 후 2일째 1.5mM/L butyrate를 함유하는 배지에서 24시간 동안 배양한 후 butyrate가 함유되지 않은 배지로 교환하여 배양하였다. 유전자 이입 후 2일, 3일, 그리고 7일에 Western blotting을 시행하여 유전자의 발현정도를 조사하였으며, butyrate 처리 유무에 따른 bystander effect에 의한 살상효과의 차이를 조사하였다. 결 과: Butyrate를 처리하지 않은 대조군은 유전자이입 후 7일째에 유전자의 발현이 없었으나 butyrate를 처리한 군은 7일째에도 유전자의 발현이 있었다. 그리고 butyrate를 처리한 경우 bystander effect에 의한 살상효과가 유의하게 증가되었다. 결 론: 이상의 결과로 butyrate는 유전자의 발현을 증가시킴에 의해 bystander effect를 증가시킴을 알 수 있었으며, 향 후 adenovirus를 이장한 유전자치료의 효과를 증가시킬 수 있는 새로운 방법이 될 수 있을 것으로 생각된다.

• Structural Engineering and Mechanics
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• 제65권3호
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• pp.291-302
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• 2018

Rigid body spring method (RBSM) is an effective tool to simulate the cracking process of structures, and has been successfully applied to investigate the behavior of reinforced concrete (RC) members. However, the theoretical researches and engineering applications of this method mainly focus on two-dimensional problems as yet, which greatly limits its applications in actual engineering projects. In this study, a three-dimensional (3-D) RBSM for RC structures is proposed. In the proposed model, concrete, reinforcing steels, and their interfaces are represented as discrete entities. Concrete is partitioned into a collection of rigid blocks and a uniform distribution of normal and tangential springs is defined along their boundaries to reflect its material properties. Reinforcement is modeled as a series of bar elements which can be freely positioned in the structural domain and irrespective of the mesh geometry of concrete. The bond-slip characteristics between reinforcing steel and concrete are also considered by introducing special linkage elements. The applicability and effectiveness of the proposed method is firstly confirmed by an elastic T-shape beam, and then it is applied to analyze the failure processes of a Z-type component under direct shear loading and a RC beam under two-point loading.

• Structural Engineering and Mechanics
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• 제12권6호
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• pp.657-668
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• 2001

Fiber reinforced cementitious composites are nowadays widely applied in civil engineering. The postcracking performance of this material depends on the interaction between a steel fiber, which is obliquely across a crack, and its surrounding matrix. While the partly debonded steel fiber is subjected to pulling out from the matrix and simultaneously subjected to transverse force, it may be modelled as a Bernoulli-Euler beam partly supported on an elastic foundation with non-linearly varying modulus. The fiber bridging the crack may be cut into two parts to simplify the problem (Leung and Li 1992). To obtain the transverse displacement at the cut end of the fiber (Fig. 1), it is convenient to directly solve the corresponding differential equation. At the first glance, it is a classical beam on foundation problem. However, the differential equation is not analytically solvable due to the non-linear distribution of the foundation stiffness. Moreover, since the second order deformation effect is included, the boundary conditions become complex and hence conventional numerical tools such as the spline or difference methods may not be sufficient. In this study, moment equilibrium is the basis for formulation of the fundamental differential equation for the beam (Timoshenko 1956). For the cantilever part of the beam, direct integration is performed. For the non-linearly supported part, a transformation is carried out to reduce the higher order differential equation into one order simultaneous equations. The Runge-Kutta technique is employed for the solution within the boundary domain. Finally, multi-dimensional optimization approaches are carefully tested and applied to find the boundary values that are of interest. The numerical solution procedure is demonstrated to be stable and convergent.