• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.274-279
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• 2015

Receptor activator of nuclear factor-kappa B ligand (RANKL) is a critical factor in osteoclastogenesis. It makes osteoclasts differentiate and multinucleate in bone remodeling. In the present study, RANKL was expressed as a soluble maltose binding protein (MBP)-fusion protein using the Escherichia coli maltose binding domain tag system (pMAL) expression vector system. The host cell E. coli DH5α was cultured and induced by isopropyl β-_D-1-thiogalactopyranoside for rRANKL expression. Cells were disrupted by sonication to collect soluble MBP-fused rRANKL. The MBP-fusion rRANKL was purified with MBP Trap affinity chromatography and treated with Tobacco Etch Virus nuclear inclusion endopeptidase (TEV protease) to remove the MBP fusion protein. Dialysis was then carried out to remove binding maltose from the cleaved rRANKL solution. The cleaved rRANKL was purified with a second MBP Trap affinity chromatography to separate unsevered MBP-fusion rRANKL and cleaved MBP fusion protein. The purified rRANKL was shown to have biological activity by performing in vitro cell tests. In conclusion, biologically active rRANKL was successfully purified by a simple two-step chromatography purification process with one column.

• Journal of Adhesion and Interface
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• v.7 no.4
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• pp.1-9
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• 2006

The single lap joint is the most studied joint in the literature in terms of both theory and practice. It is easy to manufacture and the lap shear strength is a useful value for strength assessment and quality control. Simple design rules exist such as the one present in standard ASTM 1002 or in a recent paper by Adams and Davies. The main factors that have an influence on the lap shear strength are the type of adhesive, i.e. ductile or brittle, the adherend yield strength and the overlap length. The overlap increases the shear strength almost linearly if the adhesive is sufficiently ductile and the adherend does not yield. For substrates that yield, a plateau is reached for a certain value of overlap corresponding to the yielding of the adherend. For intermediate or brittle adhesives, the analysis is more complex and needs further investigation. In order to quantify the influence of the adhesive, the adherend and the overlap on the lap shear strength, the experimental design technique of Taguchi was used. An experimental matrix of 27 tests was designed and each test was repeated three times. The influence of each variable could be assessed as well as the interactions between them using the statistical software Statview. The results show that the most important variable on the lap shear strength is the overlap length followed by the type of adherend.

• International Journal of Highway Engineering
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• v.9 no.2 s.32
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• pp.89-99
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• 2007

The stiffness of the asphalt concrete is represented by the complex modulus $E^*$, which is very important properties in the mechanistic design of flexible pavement system. The moduli of asphalt concrete were generally determined by dynamic modulus test. However, the dynamic modulus testing method is too complex, expensive, and time consuming to be applicable on a production basis. The IR(Impact Resonance) method has been shown to be a truly simple nondestructive testing method which produces very repetitive, consistent results. The major object of this study was to estimate of the effects on IR tests for determining modulus of asphalt concrete including impact position, specimen support condition, impact steel ball size and sampling rate. The variations of IR test results with various testing conditions are within ${\pm}2.7%$.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.414-420
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• 2005

The ShortStack Micro Server enables any product that contains a microcontroller or microprocessor to quickly and inexpensively become a networked, Internet-accessible device. The ShortStack Micro Server provides a simple way to add LonWorks networking to new or existing smart devices. . It implements the LonTalk protocol and provides the physical interface with the LonWorks communication. The ShortStack host processor can be an 8, 16, or 32-bit microprocessor or microcontrollers. The ShortStack API and driver typically require about 4kbytes of program memory on the host processor and less than 200 bytes of RAM. The interface between host processor and the ShortStack Micro Server may be a Serial Communication Interface (SCI). The LonWorks control module with a high performance is developed, which is composed of the 8 bit PIC Microprocessor for host processor and the smart neuron chip for the ShortStack Micro Server. This intelligent control board is verified as proceeding the various function tests from experimental system with an boost pump and inverter driving systems. It is also confirmed that the developed control module provides stably 0-10VDC linear signal to the input signal of inverter driving system for varying the induction motor speed. Thus, the experimental results show that the fabricating intelligent board carried out very well the various functions in the wide operating ranges of boost pump system. This developed control module expect to apply to industrial fields to require the comparatively exact control and monitoring such as multi-motor driving system with inverter, variable air volume system and the boost pump water supply systems.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.2
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• pp.182-189
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• 2014

This paper proposed a framework of recognition and tracking for underwater objects using sonar images as an alternative of underwater optical camera which has the limitation of usage due to turbidity. In Part 1, a design and recognition method for 2D artificial landmark was proposed considering the practical performance of current imaging sonars. In particular, its materials are selected in order to maximize detectability based on characteristics of imaging sonar and ultrasonic waves. It has a simple and omni-directional shape which allows an easy modeling of object, and it includes region based features as identifications. Also, we proposed a real-time recognition algorithm including edge detector, Hough circle transforms, and shape matrix based recognition algorithm. The proposed methods are verified by basin tests using DIDSON.

• Journal of Korean Society of Steel Construction
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• v.24 no.2
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• pp.149-161
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• 2012

The use of seismic energy-dissipative devices for passive control is increasing exponentially in the recent years for both new and existing buildings. Use of these devices started in and has been somewhat limited to developed countries. One of the current challenges is to promote the use of seismic dampers in earthquake-prone developing countries by lowering the cost of the devices. This paper proposed a new type of seismic damper based on yielding of a cantilever type metallic element for seismic retrofit of existing and new building structures. The hysteretic behavior and energy dissipation capacity of the proposed damper was investigated using component tests under cyclic loads. The experimental results indicated that the damping device had stable restoring force characteristics and a high energy dissipation capacity. Based on these results, a simple hysteretic model for predicting the load-displacement curve of the seismic damper was proposed.

• Journal of Korean Society of Steel Construction
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• v.28 no.6
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• pp.427-438
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• 2016

This study is experimental research for the effect of gap at the end plate on the performance of extended end-plate type splice. For this research, simple beam type specimens by using extended end-plate type splice are planned. Main variables are the initial gap between end-plates, the installation of finger shim plate before the installation of high tension bolts, the final gap between end-plates, and the installation of finger shim plate after the installation of high tension bolts. The static loading tests results show that the maximum bending strength of splice is not dependent on the gap, but the vertical displacement, initial stiffness and elastic stiffness are affected by the gap. In addition to that, the possibility of brittle fracture is increased when the torque of high tension bolt is used to control the gap. Thus, careful consideration is needed in this case.

• Transactions of Materials Processing
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• v.25 no.1
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• pp.12-20
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• 2016

Press machines and dies are commonly used for 3D curved sheet forming. Using conventional die forming can cause economic problems since various modifications of the die shape are required depending on the product shape. Various types of flexible forming such as multi-point dieless forming (MDF), flexible incremental roll forming have been developed to improve the needed process flexibility. Although MDF can reduce the production cost using reconfigurable dies, it still has significant material loss. Drawbacks such as wrinkling, dimpling, and forming errors can also occur despite continuous investigations to mitigate these defects. A novel sheet forming process for 3D curved surfaces, a flexibly-reconfigurable roll forming (FRRF), has been recently proposed to overcome the economic and technical limitations of current practice. FRRF has no limitation on blank size in the longitudinal direction, and also minimizes or eliminates forming defects such as wrinkling and dimpling. Feasibility studies of FRRF have been conducted using FE simulations for multi-curved shapes and various sheet thicknesses. Therefore, the fabrication of a FRRF apparatus is required for any follow-up studies. In the current study, experiments with reconfigurable rollers were conducted using a simple design pre-FRRF apparatus prior to fabricating the full size FRRF apparatus. There are three candidates for the reconfigurable roller: a bar-type shaft, a flexible shaft, a ground flexible shaft. Among these candidates, the suitable reconfigurable roller for FRRF is determined through various forming tests.

• Geotechnical Engineering
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• v.7 no.2
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• pp.51-66
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• 1991

In the stability analysis of hillside slopes, the roots of vegetation have been considered to act as a soil reinforcement. In order to predict the amount of increase in soil shear resistance, produced by tensile strength of roots that intersect a potential slip surface in hillside slopes, new soil -root interaction models are proposed in this paper. For this purpose, firstly, laboratary teats and in-situ tests wert performed on soil-root systems, and experimental results were compared with a couple of soil-root interaction models which had been proposed by Gray, Waldron, and Wu etc. Based on this comparison, a new soil-root interaction model is proposed. Secondly, a probabilistic soil-root model is proposed based on statistical analysis considering random nature of root distribution, root characteristics, and soil-root interactions. Finally, to examine the effect of this root reinforcement system on stability of hillside slopes, a simple three-dimensional stability analysis was performed, and it was shown that root reinforcement had a significant stabilizing influence on shallow slips rather than deep slips in hillside slopes.

• 연구논문집
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• s.27
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• pp.87-99
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• 1997

Present paper describes on/off design performance of a 50KW turbogenerator gas turbine engine for hybrid vehicle application. For optimum design point selection, relevant parameter study is carried out. The turbogenerator gas turbine engine for a hybrid vehicle is expected to be designed for maximum fuel economy, ultra low emissions, and very low cost. Compressor, combustor, turbine, and permanent-magnet generator will be mounted on a single high speed (82,000 rpm) shaft that will be supported on air bearings. As the generator is built into the shaft, gearbox and other moving parts become unnecessary and thus will increase the system's reliability and reduce the manufacturing cost. The engine has a radial compressor and turbine with design point pressure ratio of 4.0. This pressure ratio was set based on calculation of specific fuel consumption and specific power variation with pressure ratio. For the given turbine inlet temperature, a rather conservative value of $1100^\circK$ was selected. Designed mass flow rate was 0.5 kg/sec. Parametric study of the cycle indicates that specific work and efficiency increase at a given pressure ratio and turbine inlet temperature. Off design analysis shows that the gas turbine system reaches self operating condition at N/$N_{DP}$ = 0.53. Bleeding air for turbine stator cooling is omitted considering low TIT and for a simple geometric structure. Various engine performance simulations including, ambient temperature influence, surging at part load condition. Transient analysis were performed to secure the optimum engine operating characteristics. Surge margin throughout the performance analysis were maintained to be over 80% approximately. Validation of present results are yet to be seen as the performance tests are scheduled by the end of 1998 for comparison.