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The Effect of Alcohol Fermented Feedstuff Made of Byproducts on Fermentation Characteristics and Dry Matter Disappearance in the Rumen (비지박 및 맥주박을 이용한 알코올 발효사료의 반추위내 발효특성 및 건물 분해율에 미치는 영향)

  • Shin, Jong-Suh;Lin, Guang Zhe;Kim, Byong-Wan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.28 no.1
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    • pp.49-60
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    • 2008
  • A study was conducted to determine the effect of alcohol-fermented feedstuff formulated with byproducts on the fermentation characteristics and dry matter disappearance in the rumen. Dietary treatments were either a soybean curd-based alcohol-fermented feedstuff(AFS) and brewery grain-based alcohol-fermented feedstuff(AFB). The AFS and AFB are composed of 50% commercial beef cattle feed, 50% soybean curd dreg, 5% molasses and 0.5% yeast, and 25% commercial beef cattle feed, 25% brewery grain, 25% soybean curd dreg, 25% corn grit, 5% molasses and 0.5% yeast, respectively. The ruminally cannualted Korean cattle were utilized to investigate the change of ammonia, pH alcohol, volatile fatty acids, and DM digestibility at 0, 2, 4, 8 and 12 hr after feeding. The rumen ammonia concentrations were significantly lower in AFS and AFB with incubation time, especially at 6 hr incubation(AFS, 0.7 mg/dl; AFB, 1.5 mg/dl; control 2.5 mg/dl). Lower rumen pH was observed in AFS and AFB during the early stage of incubation, but no significant difference was found at late stage of incubation. The total VFA concentrations were not affected by diet treatments at 2 hr incubation time, but the concentration significantly decreased after that. The dry matter disappearance was significantly lower in AFS and AFB during the early stage of incubation. However, the dry matter disappearance of AFS and AFB was similar to that of control during the late stage of incubation. It is concluded that the industrial byproducts such as soybean curd dreg and brewery grain were effective materials to make an alcohol fermented feedstuffs and resulted in better fermentation characteristics in the rumen when both were applied to Hanwoo.

A Study on Trace-metals in Korean Yeongdeok Crab and Russian Snow Crab (영덕대게와 러시아산대게의 체내 미량금속 함량 연구)

  • Kim, Cho-Ryeon;Yoon, Yi-Yong
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.14 no.3
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    • pp.147-153
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    • 2011
  • This study is to measure the differences of the trace-metals resulted from the different ecologies, such as nickel(Ni), copper(Cu), zinc(Zn), cadmium(Cd), plumbum(Pb), arsenic(As) and chrome(Cr) remaining in the parts of Korean Yeongdeok Crab (KYC) and Russian Snow Crab (RSC) based on ICP-MS. The recovery rate of each metal certified the reference materials (CRM) was in the average of 81~99%, which corresponded with the level required in Codex. The level of metals in the parts of KYC was in the order of Ni > As > Zn > Cu > Cr > Cd in the both male and female shell; the order of Zn > As > Cu > Cr > Ni > Cd in the leg flesh; the order of Zn > As > Cu > Cr > Cd > Ni in the body flesh; the order of Cu > Zn > As > Cd > Cr > Ni in the gill; the order of Cu > As > Zn > Cd > Ni > Cr in the male hepatopancreas; the order of Cu > Zn > As > Cd > Cr > Ni in the female hepatopancreas, thereby showing some differences. It was revealed that the levels of most metals (nickel, copper, zinc, arsenic and chrome) were similar between KYC and RSC except cadmium which was somewhat lower than that of KYC. However, the cadmium in RSC was discovered in high level in most of the parts, two times higher in the hepatopancreas, and four times in the gill. It was also revealed that the trace metal contents were changing according to the size of KYC; the metals with the highest level of Ni in shell, Zn in leg and body flesh, Cu in gill tended to decrease as growing, whereas the cadmium contents tended to increase overall and accumulated the most in hepatopancreas. The results showed there was a possibility that the phenomenon of bioaccumulation within hepatopancreas would increase as growing.

Effect of Selenium on Internal Quality of Basil (Ocimum basilicum L.) During Storage (Basil 양액재배 시 Selenium 첨가가 저장 중 품질에 미치는 영향)

  • 박권우;김민순;강호민;이문정
    • Journal of Bio-Environment Control
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    • v.9 no.4
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    • pp.193-200
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    • 2000
  • The object of this study is to investigate the quality change of sweet basil grown with selenium(Se) in hydroponic culture. Sweet basil was cultured with 1 fold herb nutrient solution as suggested by European vegetable R & D Center in Belgium. Before three weeks harvest, sodium selenate(N $a_2$Se $O_4$) was supplied to 2 and 4 mg. $L^{-1}$ in the nutrient solution. Sweet basil was stored at 1$0^{\circ}C$ using 40um ceramic film and PET (polyethylene terephalate) for 15 days in modified atmosphere(MA) storage condition. The weight loss of sweet basil was higher in non-treatment compared to Se treatments in both of two films but it was decreased over 5% in PET treatment. Se concentrations in leaf tissues increased in the response to the treated levels of N $a_2$Se $O_4$concentrations, and this tendency was appeared similar results after storage. There was no significant effect of packing materials on volatilization of Se in sweet basil. The total chlorophyll and essential oil content was increased with increasing N $a_2$Se $O_4$concentration in nutrient solution. The amount of volatilization flavor was not higher at N $a_2$Se $O_4$4mg. $L^{-1}$ treatment compare to others during storage. Se content was 112.73 ug. $g^{-1}$ dry mass at 2 mg. $L^{-1}$ treatment before storage and the decrease of Se content was observed by 50% at 15 days after storage. The condition, which N $a_2$Se $O_4$2mg. $L^{-1}$$^{plement}$ in nutrient solution during growth stage and stored with 40um ceramic film on 1$0^{\circ}C$are acceptable for maintaining of sweet basil quality. Moreover it can be a proper Se concentration for human health. Overall, Se treatment in nutrient solution has effect on promoting and maintaining quality of herb during storage life. Also, there was not significant change of essential oil compounds by volatilization of Se.mpounds by volatilization of Se.

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Kinetics of esterification of food waste oil by solid acid catalyst and reaction optimization (고체 산 촉매를 이용한 고산가 음폐유의 에스테르화 반응 동역학 연구 및 반응 최적화)

  • Lee, Hwa-Sung;Lee, Joon-Pyo;Lee, Jin-Suk;Kim, Deog-Keun
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.3
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    • pp.683-693
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    • 2017
  • Transport biofuels have been recognized as a promising means to resolve the following issues like global warming, oil depletion and environmental pollutions. Among various biofuels, biodiesel has several advantages such as less emission of air pollutants and higher cetane values compared to diesel oil. Demand for biodiesel in Korea is increasing that leads to higher dependence on the imported feedstocks. Therefore, it is important to utilize the waste materials collected domestically for biodiesel production. Food waste oil collected in waste treatment facility has not been used for biodiesel production due to high free fatty contents in the oil. In this work, biodiesel conversion of food waste oil by Amberlyst 15 was studied. Synthetic and actual food waste oils have been used in the study. First, the effects of the major operating parameters including reaction temperature, methanol to oil molar ratio and catalyst loading on the conversion rates and yields were determined with synthetic waste oil. Kinetic modelling work was also done to determine the activation energy of the reaction. From the work, optimization reaction conditions were determined to be 383K, 1: 26.1 for methanol molar ratio to oil, 10 wt.% for catalyst loading and 360 min for reaction time. Activation energy of the reaction is determined to be 29.75 kJ/mol, lower than those reported in the previous works. So the solid catalyst, Amberlyst 15, was more efficient for esterification than the solid catalysts employed in the other works. Agitation rates have the negligible effects on the conversion rates and yields. With the identified optimization conditions, conversion of the actual food waste oil was also carried out. The esterification yield of actual food waste oil in 60 min was 13% lower than that of synthetic waste oil but the final yields in 240 min were similar each other, 98.12% for synthetic oil and 97.62% for actual waste oil.

Material Characteristics and Provenance Presumption for Stone Artifacts of Bronze Age from the Hyocheon Site in Gwangju, Korea (광주 효천유적 출토 청동기시대 석기의 재질특성과 원산지 추정)

  • Park, Sung-Mi;Lee, Chan-Hee;Kim, Ji-Young;Jeong, Il
    • Journal of Conservation Science
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    • v.21
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    • pp.5-20
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    • 2007
  • The stone artifacts in Bronze age from the Hyocheon sites in the Gwangju, Korea were studied on the basis of material characteristics and provenance presumptions. The use and rock names of the artifacts are a stone shovel by andesite, the stone grinding pestle by pyrophyllite, the stone sickle by schist and four stone semifinished artifacts by slates. Andesitic stone shovel could be observed easily around the Hyocheon relic site. But, rocks of the stone grinding pestle, the stone sickle, the stone arrowhead and the stone semifinished artifacts could be confirmed typical occurrences of the all kinds of rocks around the Hwasun coal mine area above 10km from the site. These are made the coupled samples with each stone artifact to the same kinds of raw material rocks based on analysis of the lithology and geochemistry. As a result a geochemical evolution trends of both a stone artifact and the rock showed very similar patterns based on normalization using the behavior, enrichment, compatibility and incompatibility of the elements. Therefore, the source rock of the stone shovel was convey from Mudeung mountain possible interpreted that the domestic-type artifacts are distributed in the vicinity of the Hyocheon site. On the other hand, the stone grinding pestle, the stone sickle, the stone arrowhead and the stone semifinished artifacts were convey from the Hwasun coal mine area possible foreign-type stone artifacts interpreted that the source rocks. Consequently, in the foreign-type stone artifacts are should archaeologic research which it can examine various possibilities clearly that the possibility to coming the introduction with the mankind migration, diffusion to dealings of tribe, the captured enemy equipment through the war and the trade with the behavior of the materials.

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The Usefulness Assessment of Verifying Daily Output by Using CHECKMATE$^{TM}$ (CHECKMATE$^{TM}$를 이용한 일일 출력 검증의 유용성 평가)

  • Cho, Han-Sang;Nam, Sang-Soo;Park, Hae-Jin;Kim, Mi-Hwa;Park, An-Tae
    • The Journal of Korean Society for Radiation Therapy
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    • v.23 no.1
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    • pp.51-58
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    • 2011
  • Purpose: In this study, we tried to check the usefulness of two Linear Accelerators, Clinac IX and 21EX (Varian, Palo Alto, CA), which are equipped in Ajou Medical Center. From 2008 to 2010, we evaluated the error range of Absolute Dose based on the daily output, which was measured by CHECKMATE$^{TM}$ (Sun Nuclear, Melbourne, FL). Materials and Methods: For Daily Q.A, photon beams of two linear accelerators, 21EX and IX (6 MV and 10 MV, respectively) were measured daily by using CHECKMATE$^{TM}$ just before the treatment began, while the absolute dose was measured biweekly by using water phantom. We analyzed the data of measured values from the daily Q.A and the absolute dose from 2008 to 2010 for 21EX, and from 2009 to 2010 for IX. We utilized Excel 2007 (Microsoft, USA) to evaluate Average, Standard deviation and Confidence level of the data. Furthermore, in order to check the measured values of CHECKMATE$^{TM}$ and the significance of absolute dose, each error value was compared and analyzed. Results: During the observation period, the output of two equipment's absolute dose increased in process of time and in both 6 MV and 10 MV, there was a similar increasing trend. In addition, the error rate of the measured value of CHECKMATE$^{TM}$ and the value of absolute dose were under 0.34, which means that there is a similarity relationship between the two measured values. After checking that the measured value of CHECKMATE$^{TM}$ increased, We measured the absolute dose to adjust that. When the error range was close to 2~3%, the number of changing the output was four for 21EX and three for IX. Conclusion: As a result of measuring and analyzing the daily output changes for two years by using CHECKMATE$^{TM}$, we could find that there is a significance between the output which we should obey during Q.A, and the measured value of absolute dose within the error tolerance of 2~3%. Thus, the use of CHECKMATE$^{TM}$ can be positively considered for more efficient and reliable daily output verification of linear accelerator. It can also be a good standard for other medical centers to understand the trends of linear accelerator and to refer to for the correction of each output.

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Clinical and Histopathological Study in Repaired Cartilage after Microfracture Surgery in Degenerative Arthritis of the Knee (퇴행성 슬관절염에서 미세 천공술후 재생된 연골의 임상 및 병리조직학적 연구)

  • Bae, Dae-Kyung;Yoon, Kyoung-Ho;So, Jae-Keun
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.4 no.1
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    • pp.18-28
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    • 2005
  • Purpose: The purpose of this study is to evaluate the clinical, radiological and histopathological results after microfracture surgery for degenerative arthritis of the knee. Materials and Methods: From Oct. 1997 to Dec. 1998, 48 knees in 46 patients were treated by microfracture technique. Their mean age at the time of operation was 56 years(range, 40-75 years) and mean period of follow-up study was one year(range, 7-20 months). For 24 knees in 22 patients, 'second-look' arthroscopies and biopsies were performed at 6 months following microfracture. At the last follow up clinical results were evaluated with Baumgaertner's scale. The specimens of 24 cases were stained with H-E, Safranin-O, and Masson's trichrome. Eighteen of 24 cases were stained immunohistochemically and the Western blotting test was performed on 12 cases for type II collagen. We analyzed the relationship of the Western blotting for type II collagen with clinical score, preoperative varus deformity, joint space widening in radiological result, extent of repaired articular cartilage in '2nd-look' arthroscopic findings, patient's age and weight. Results: Clinical results were excellent in 90% and good in 10%. Among the 24 knees, more than 80% of areas of chondral defect were covered with regenerated cartilage in 21 knees Histologically, the repaired tissue appears to be a hybrid of hyaline cartilage and fibrocartilage. Repaired cartilage contains variable amounts of type II collagen with immunohistochemical staining. The results of the Western blotting test were similar. The amounts of type II collagen formation had positive correlation with the extent of repaired cartilage and preoperative varus deformity. Conclusion: 'Second-look' showed that the chondral defect areas were covered with newly grown grayish white tissue. Articular cartilage repair was confirmed with histological and immunohisto-chemical study qualitatively, and the amount of type II collagen was calculated with the Western blotting test quantitatively. The exact nature and fate of repaired cartilagenous tissues need further long term follow-up study. The results of this study provide the rationale to select osteoarthritic patients indicated for microfracture surgery.

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A comparison between the modified Brostrom procedure using single and double suture anchor for chronic lateral ankle instability (발목관절 외측 불안정성에서 단일 봉합나사와 이중 봉합나사를 이용한 변형 Brostrom 술식간의 임상결과 비교)

  • Shon, Hyun-Chul;Cho, Byung-Ki;Kim, Yong-Min;Kim, Dong-Soo;Choi, Eui-Sung;Park, Kyoung-Jin;Park, Ji-Kang
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.10 no.2
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    • pp.69-77
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    • 2011
  • Purpose: This study was retrospectively performed to compare the clinical outcomes of modified Brostrom procedures using the single suture anchor and the double suture anchor for chronic lateral ankle instability. Materials and Methods: Thirty-seven patients were followed up for more than 1 year after the modified Brostrom procedures using suture anchor. Single surgeon treated seventeen cases with single suture anchor and 20 cases with double suture anchor. The clinical evaluation was performed according to the Karlsson scale and Sefton grading system. Radiologic measurement of the talar tilt and anterior talar translation was performed through anterior and varus stress radiographs using Telos device. Results: The Karlsson scale had improved significantly from preoperative average of 45.2 points to 89.4 points in single suture anchor group, and from 46.4 points to 90.5 points in double suture anchor group. According to the Sefton grading system, 15 cases (88.2%) in single suture anchor group and 18 cases (90%) in double suture anchor group achieved satisfactory results. The talar tilt angle and anterior talar translation had improved significantly from preoperative average of $13.6^{\circ}$ and 8.6 mm to $5.4^{\circ}$ and 4.1 mm in single suture anchor group, from $14.1^{\circ}$ and 8.4mm to $3.9^{\circ}$ and 4 mm in double suture anchor group. Double suture anchor technique was significantly superior in postoperative talar tilt. Conclusion: Single and double suture anchor techniques produced similar clinical and functional outcomes except for talar tilt, which was significantly superior in double suture anchor group. Both modified Brostrom procedures using the single and double suture anchor appear to be effective treatment methods for chronic lateral ankle instability. Further evaluation of clinical outcomes and biomechanical studies in athletes are needed.

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Barrier Techniques for Spinal Cord Protection from Thermal Injury in Polymethylmethacrylate Reconstruction of Vertebral Body : Experimental and Theoretical Analyses (Polymethylmethacrylate를 이용한 척추체 재건술에서 척수의 열 손상을 방지하기 위한 방어벽 기법 : 실험적 및 이론적 분석)

  • Park, Choon Keun;Ji, Chul;Hwang, Jang Hoe;Kwun, Sung Oh;Sung, Jae Hoon;Choi, Seung Jin;Lee, Sang Won;Park, Sung Chan;Cho, Kyeung Suok;Park, Chun Kun;Yuan, Hansen;Kang, Joon Ki
    • Journal of Korean Neurosurgical Society
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    • v.30 no.3
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    • pp.272-277
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    • 2001
  • Objective : Polymethylmethacrylate(PMMA) is often used to reconstruct the spine after total corpectomy, but the exothermic curing of liquid PMMA poses a risk of thermal injury to the spinal cord. The purposes of this study are to analyze the heat blocking effect of pre-polymerized PMMA sheet in the corpectomy model and to establish the minimal thickness of PMMA sheet to protect the spinal cord from the thermal injury during PMMA cementation of vertebral body. Materials & Methods : An experimental fixture was fabricated with dimensions similar to those of a T12 corpectomy defect. Sixty milliliters of liquid PMMA were poured into the fixture, and temperature recordings were obtained at the center of the curing PMMA mass and on the undersurface(representing the spinal cord surface) of a prepolymerized PMMA sheet of variable thickness(group 1 : 0mm, group 2 : 5mm, or group 3 : 8mm). Six replicates were tested for each barrier thickness group. Results : Consistent temperatures($106.8{\pm}3.9^{\circ}C$) at center of the curing PMMA mass in eighteen experiments confirmed the reproducibility of the experimental fixture. Peak temperatures on the spinal cord surface were $47.3^{\circ}C$ in group 2, and $43.3^{\circ}C$ in group 3, compared with $60.0^{\circ}C$ in group 1(p<0.00005). So pre-polymerized PMMA provided statistically significant protection from heat transfer. The difference of peak temperature between theoretical and experimental value was less than 1%, while the predicted time was within 35% of experimental values. The data from the theoretical model indicate that a 10mm barrier of PMMA should protect the spinal cord from temperatures greater than $39^{\circ}C$(the threshold for thermal injury in the spinal cord). Conclusion : These results suggest that pre-polymerized PMMA sheet of 10mm thickness may protect the spinal cord from the thermal injury during PMMA reconstruction of vertebral body.

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Evaluation of Usefulness on In-vivo Diode Dosimetry for Measuring the Tumor Dose of Oral Cancer Patient (구강암 환자의 종양 선량 측정을 위한 In-vivo Diode Dosimetry의 유용성 평가)

  • Na Kyung-Su;Lee Je-Hee;Park Heung-Deuk
    • The Journal of Korean Society for Radiation Therapy
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    • v.17 no.2
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    • pp.133-140
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    • 2005
  • Purpose : This test is designed to identify the validity of treatment plan by implementing real-time dosimetry by means of dose that is absorbed into PTV and OAR when preparing doses of 3D and POP plans. Materials and Methods : In treatment. error can be calculated be comparing Exp. Dose with the actual dose, which has been converted from 'the reading value obtained by placing diode detector on the area to be measured'. Same test can be repeated using Alderson-Rando phantom. Results : Errors were found: A patient(POP plan): 197.6/199=-1.2%, B patient(3D-plan): 199.9/198.7=+0.6%, C patient: 196/200=-1.5%. In addition, considering the resulted value of measuring OAR besides target-dose for C patient showed 96/200, representing does of 47%, the purpose of protection was judged to be duly accomplished. Also it was acknowledged the resulted value of -3.7% met the targeted dose within the range of ${\pm}5%$. Conclusion : Aimed for identifying the usefulness of pre-treatment dose measurement using diode detector, this test was useful to evaluate the validity of curing because it resulted in the identification of category to be protected as well as t dose. Moreover, it is thought to have great advantage in ascertaining the dose of target, dose of which is not calculated yet. Similar to L-gram before treatment, this test is thought to be very effective so that it can bring great advantages in the aspects such as validity of curing method and post-treatment plan as well.

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