• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.1
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• pp.65-69
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• 2010

This study was carried out to investigate the antioxidative and anti-inflammatory activity of crude anthocyanin compounds extracted from black soybean. The crude anthocyanin compounds were extracted with 80% methanol and concentrated to powder. The most abundant compound isolated from the extract was C3G(cyanidin-3-glucoside). The superoxide dismutase (SOD) assay was conducted to assess the antioxidative activity of the crude extract. SOD, which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. The black soybean anthocyanin extracts inhibited more than 90% of the superoxide radical at a concentration of 0.1% and 100% at a concentration of 0.5%, indicating that this extract displayed excellent antioxidative activity. To assess the anti-inflammatory activity of the extract, a NO(Nitric oxide) production assay in RAW 264.7 cells was performed. NO is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. In this assay, the anthocyanin extracts showed a high anti-inflammatory potential, where the inhibitory potency for NO production was similar to the positive control, particularly for EGCG(epigallocatechin-3-gallate), which is known to have excellent anti-inflammatory activity. Thus, it can be concluded that the anthocyanin extracts from black soybean have distinctive pharmaceutical activities and may be used as an excellent source materials to supplement the health benefits of various food products.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.86-97
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• 2018

The objective of this study was to determine the effects of low temperature on starch accumulation in rice grains. We used four major Japonica-type Korean rice cultivars as materials: Jinbu (JB), Junamjosaeng (JJ), Geumyoung (GY), and Hwawang (HW). Rice plants were moved into two phytotrons the day after heading. Temperatures in the two phytotrons were maintained at $19/29^{\circ}C$ (night/day) as the control, and $13/23^{\circ}C$ as the low temperature condition, both under natural daylight with a relative humidity of 65%. The ripening rates of JB and JJ showed no significant difference between the low temperature and control conditions at 45 days after heading (DAH). In contrast, the ripening rates of GY and HW were 86% and 57% lower than those of JB and JJ under the low temperature condition at 45 DAH, respectively. However, the ripening rates of these four varieties at 61 DAH (when accumulated temperature reached $1,100^{\circ}C$) under the low temperature condition were similar to those at 45 DAH under the control condition (JB, 94%; JJ, 97%; GY, 97%; HW, 88%). The total starch contents showed no significant difference between the control and low temperature conditions. However, the amylose contents in the cultivars were higher under the low temperature than under the control condition. The enzyme activities of starch biosynthesis were about 5-10 days slower in cultivars under the low temperature than under the control. The grain-filling rate showed significant correlations with the enzyme activities of SuSase ($r^2=0.70^{***}$), AGPase ($r^2=0.63^{***}$), UDPase ($r^2=0.36^{***}$), StSase ($r^2=0.51^{***}$), and SBE ($r^2=0.59^{***}$). In conclusion, although StSase activity was increased at $13/23^{\circ}C$ up to 20 DAH, there might not be enough time for SBE to synthesize amylopectin, thus affecting the amylose content of HW, which had the slowest grain filling rate. Notably, the decreased activity of SuSase and SBE and late increase in AGPase activity under the low temperature during the ripening stage are considered to be disadvantageous, as they delay ripening and increase the amylose content.

• Economic and Environmental Geology
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• v.51 no.1
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• pp.49-65
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• 2018

The lime-soil mixture on tomb barrier (LSMB) is a type of tomb in Joseon Dynasty, which made with so-called 'Sammul' (three material compound) that mixture of lime, fine sand and yellow ocher. This study divided the tombs of the Gungri Site from Joseon Dynasty with layered wall and integrated wall according to the manufacturing types, and investigated on the basis of analysis to material characteristics and making techniques. Analytical samples were classified with lime-soil mixtures and soils, and interpreted the mixing characteristics of Sammul based on types of tomb barrier. The tomb barrier which is directly effect to control the inner environment was made with high content of lime. But the finishing or bottom layer were made with low content of lime. Overall the LSMB with integrated wall has higher content of lime and physical property than the LSMB with layered wall. The soil which was compounded as a Sammul and collected near the Gungri Site had similar with mineralogical and geochemical characteristics. Therefore, it is presumed that the fine sand and yellow ocher that made as a Sammul, were used with soil that was distributed around the site. Meanwhile, large scale limestone quarry is distributed near the site. Especially, Gungri Site has a possibility of material supply through water transport, due to the sea route from Asan bay is connected near the site. Thus, there is the possibility of transportation of lime materials from nearby quarry.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.337-342
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• 2008

The nitrogen (N) and phosphorus (P) balances in Korea during 1985-2006, which calculated according to the surface balance method of the PARCOM guidelines, continually increased with the lapse of year, and then might keep the highest levels with about $250kg\;N\;ha^{-1}$ and $50kg\;P\;ha^{-1}$ in 2006 among OECD countries. National phosphorus balance of the developed OECD countries (Belgium, Denmark, Germany, The Netherlands) in the livestock feeding industries has significantly decreased, due to limit livestock feeding density and nutrient application level by using strong legislation. However, Korean P balance was not apparent decrease, since the number of livestock feeding heads has been increasing, irrespective with the decrease of chemical fertilizer consumption. Manure P portion to the total P input amount of the above four OECD Countries in nutrient balance has continuously inclined to 60-70%. Therefore, the large portion of livestock manure might be used as nutrient source in agricultural side in these countries. In particular, manure P application levels of Belgium and The Netherlands, which have relatively high P balance and manure P portion to total P input dose, was estimated to be $31-33\;kg\;p\;ha^{-1}$ in 2004, and it was similar level with that of Korea in 2006. The manure P application levels for agricultural purpose has continuously decreased in these two OECD countries. In contrast, our manure P application rate continuously increased, and therefore we need the strong political countermeasure to control livestock feeding density at the resonable level.

• Korean Journal of Food Science and Technology
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• v.46 no.6
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• pp.689-693
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• 2014

Flounder sikhe is one of the traditional Korean fermented foods. Because honey, fucoidan, propolis, aronia berry, and horseradish are well known to have natural antimicrobial properties, we investigated their antimicrobial effects on improving the quality of flounder sikhe. Ethanol extracts of propolis inhibited the growth of Bacillus cereus, Staphylococcus aureus, and Listeria monocytogenes. The extracts of aronia berry showed similar effects against Salmonella Typhimurium. We also assessed the total polyphenol and total flavonoid content and 2,2-diphenyl-2-picrythydrazyl (DPPH) radical scavenging activity in the extracts obtained from these natural materials. The contents of polyphenols and flavonoids were the highest in propolis, which also showed a high DPPH radical scavenging activity. Therefore, addition of propolis 0.5% to flounder sikhe showed optimal improvement, with a storage stability of 15 days at pH 5.39, volatile basic nitrogen (VBN) content at 24.73 mg/100 g, and total bacterial count at $1.81{\times}10$ colony forming unit (CFU)/g.

• Korean Journal of Head & Neck Oncology
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• v.30 no.2
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• pp.53-61
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• 2014

Background and Objectives : Anaplastic thyroid carcinoma(ATC) is a rare but highly aggressive thyroid malignancy that is associated with an extremely poor survival despite the best multidisciplinary care. BRAF(V600E) mutation is detected in about a quarter of ATC, but unlike its high treatment response to selective BRAF inhibitor (PLX4032) in metastatic melanoma, the treatment response of ATC is reported to be low. The purpose of this study is to investigate the innate resistance mechanism responsible for this low treatment response to BRAF inhibitor and its effect on epithelial-mesenchymal transition(EMT). Materials and Methods : Two ATP cell lines, 8505C and FRO were selected and treated with PLX4032 and its drug sensitivity and effects on cell migration and EMT were examined and compared. Further investigation on the changes in signals responsible for the different treatment response to PLX4032 was carried out and the same experiment was performed on both orthotopic and ectopic xenograft mouse models. Results : FRO cell line was more sensitive to PLX4032 treatment compared to 8505C cell line. The resistance to BRAF inhibition in 8505C was due to increased expression of EGFR. Effective inhibition of both EGFR and p-AKT was achieved after dual treatment with BRAF inhibitor(PLX4032) and EGFR inhibitor(Erlotinib). Similar results were confirmed on in vivo study. Conclusion : EGFR-mediated reactivation of the PI3K/AKT pathway and MAPK pathway contributes to the relative insensitivity of BRAF(V600E) mutant ATC cells to PLX4032. Dual inhibition of BRAF and EGFR leads to sustained treatment response including cell invasiveness.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.5
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• pp.574-589
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• 2006

Although concentrations of hazardous air pollutants(HAPs) are very low in the atmosphere, a growing attention has been paid on such compounds due to their high toxicity and bioaccumulation potentials into human body. In order to control and manage the amount of these materials in ambient air, it is necessary to construct monitoring system of them and to know the current concentration level of HAPs above all. In this work, a wide range of HAPs has been measured in metropolitan area to recognize the present state of HAPs in this area. The measured concentration of VOCs was higher in order of Jeonnongdong, Jeongdong, and Yangsuri. The regional difference of VOCs concentration was also highest in spring. Its total VOCs was ranged from $15.17{\sim}41.45$ ppb. Benzene $0.43{\sim}2.32$ ppb showed similar concentration level with the result of previous researches in Seoul. This value is a little higher than the average concentration 0.92 ppb for national ambient air quality standards in Japan. The concentration of aldehydes in this study was lower than those of other researches. Previous works in Seoul metropolitan area showed that the concentration of formaldehyde and acetaldehyde were higher than 5 ppb. The concentration of gaseous and particulate PAHs was high in order of winter, spring, and summer More than 90% of PAHs with low molecular weight such as 2-rings and 3-rings PAHs existed in gas phase. On the other hands, PAHs with high molecular weight more than 5-rings PAHs almost existed in particulate. In spring, the concentration of gaseous PAHs was 24.38 $ng/m^3$ in Jeongdong. Among the particulate PAHs, the concentrations of Naphthalene, Benzo(b)fluoranthene, and Benzo(g, h, i)perylene were higher than others. Especially, the concentration of Benzo(a)pyrene, a important carcinogenic pollutant, was highest in winter 0.5 $ng/m^3$ and ranged from 0.03 to 0.3 $ng/m^3$ in spring and summer, which is lower than the monitoring result in 90's. These components were mainly originated from the vehicle exhaust or heating equipment use.

• Economic and Environmental Geology
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• v.35 no.2
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• pp.137-147
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• 2002

Natural geological and environmental processes reflect to element abundances in geological materials on the surface. This study aims to elucidate a possibility of geostatistical application to differentiate geochemical anomalies affected by anthropogenic and geogenic factors. A regional geochemical map was produced using 'inverse distance weight interpolation' method for analytical results of stream sediments «150 11m) which were collected from 2,290 first- to second-order streams over the whole Gyeonggi Province. The Jurassic granitic batholith in the southeastern province was selected as a target for the geostatistical examination. Factor analysis was conducted using 22 elements for stream sediments from 445 drainage basins over the granitic body. Co, Cr, Sc, MgO, Fe$_{2}$O$_{3}$, V, and Ni were grouped with high correlation coefficients and the depletion of the components may reflect the whole-rock chemistry of the granite. Regression analysis was done using Co, Cr, and Sc as dependent variables and other six components as independent variables, and the results were drawn as maps. The maps acquired generally show quite similar distribution patterns with those of concentrations of each variable. The similarity in the spatial patterns between the two maps indicates that the application of regression statistics can be valid for the interpretation of regional geochemical data. However, some components show local discrepancies which may be influenced by secondary factors regardless of the basement lithology. The regression analysis may be effective in extracting local geochemical anomalies which may reflect rather anthropogenic pollutions than geogenic influences.

• Economic and Environmental Geology
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• v.26 no.4
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• pp.489-498
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• 1993

The Okbang amphibolites occurring as sill-shaped bodies within the Precambrian Wonnam Group have been studied in terms of geochemical characteristics for their tectonomagmatic environments. The amphibolites fall in the ortho-amphibolite fields in Ni and Cr versus Cu diagrams. They belong to subalkaline and tholeiitic series in total alkali versus silica and ternary AFM diagrams, respectively. They show the compositional variation corresponding to the differentiation trend of tholeiitic suites. In discrimination diagrams using high-field-strength elements such as Ti, Zr, Nb and Y, the amphibolites show geochemical affinities to both of volcanic-arc tholeiites and normal (depleted) mid-oceanic ridge tholeiites. The REE patterns of the amphibolites are nearly flat and extremely similar to those of back-arc tholeiites. $(La/Yb)_{CN}$ ratios vary from 0.89 to 2.02 with an average value of 1.23. Such low light-REE abundances in the amphibolites suggest that they were derived from the upper mantle source depleted in these elements. In view of geochemical characteristics showing strong enrichments of incompatible elements such as K and Rb, distinctive negative Nb anomalies, depletions of light-REE observed also in normal (depleted) mid-oceanic ridge tholeiites, and unfractionated immobile elements such as Y and Yb, the tholeiitic magmas, from which the parent rocks of the amphibolites were formed, would be generated from a depleted upper mantle source and contaminated by continental crustal materials en route to surface. Tectonomagmatic environment for the amphibolites can be assumed to be continental back-arc basin.

• Journal of Korean society of Dental Hygiene
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• v.6 no.3
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• pp.187-201
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• 2006

The purpose of this study is to examine the satisfaction for college life and oral prophylaxis subject and improve the satisfaction for this subject by satisfying students' desire through the results of correlation, and to inspire a professional sense by helping students enjoy healthy college life. In order to achieve these purposes, 127 senior students of the Dental Hygiene Department, Kwangju Health College took part in this study to examine the satisfaction for a study course, a lab, professors, a college, and subjects. The following results were obtained. 1. The overall satisfaction for the Dental Hygiene Department was 2.83 and the highest dissatisfaction factors included difficult subjects and non-aptitude. The overall satisfaction for professors was 3.33 and students wanted professors' human contact with students and a well-prepared lecture. The overall satisfaction for college life was 2.65, suggesting not very high satisfaction, and the highest dissatisfaction factors included similar life to high school and surrounding environment. 2. The overall satisfaction for theory was 3.83 and for practice was 3.91 in oral prophylaxis. While the highest satisfaction was found in 'the objective and direction of class' and 'class and a supplementary lesson in not giving a lecture', the lowest satisfaction was found in 'experience in reading textbook-related materials or books'. 3. The correlation of the satisfaction of theory and practice of oral prophylaxis was examined. The satisfaction of oral prophylaxis theory was positively correlated with a school course, professors, and college, while the satisfaction of oral prophylaxis practice was positively correlated with a school course, professors, college, satisfaction of oral prophylaxis theory, and present health condition(p<0.05). 4. Factors influencing the satisfaction for the theory of oral prophylaxis included professors, while factors influencing the satisfaction for the practice were statistically significant in professors and health condition(p<0.05). Based on these results, in order for professional dental hygienists to be developed as professionals serving society, it is recommended to enhance the satisfaction for an oral prophylaxis subject and that professors should provide warm-hearted support and become a role model as an educator.