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Expression of Luteinizing Hormone (LH) Gene in Rat Uterus and Epididymis (흰쥐 자궁과 부정소에서의 Luteinizing Hormone (LH) 유전자 발현)

  • Lee, Sung-Ho;Lee, Young-Ki
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.2
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    • pp.157-161
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    • 1999
  • Recent studies clearly demonstrated that the novel expression of LH gene in the rat testis, and suggested the local action of the LH-like molecule. The present study was performed to analyze the expression of LH genes in the rat accessory reproductive organs. Expression of LH subunit genes in the rat uterus and epididymis was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and specific LH radioimmunoassay (RIA). The $LH_{beta}$ transcripts in these organs contained the published cDNA structure, the pituitary type exons 1-3, which encoded the entire $LH_{beta}$ polypeptide. Presence of the transcripts for the ${\alpha}$-subunit in the rat reproductive tissues were also confirmed by RT-PCR. In the LH RIA, significant levels of LH were detected in crude extracts from the rat ovary, uterus and epididymis. The competition curves with increasing amount of tissue extracts were parallel with those of standard peptide, indicating that the immunoreactive LH-like materials in these tissues are similar to authentic pituitary LH molecule. In rat epididymis, the highest amount of immunoreactive LH was detected in corpus area. Our findings demonstrated that the genes for LH subunits are expressed in the rat accessory reproductive organs, and suggested that these extrapituitary LH may act as a local regulator with auto and/or paracrine manner.

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Evaluation of Seismic Performance on Shear Walls in Steel House (스틸하우스 전단벽체의 내진성능평가)

  • 이재석;이승은;홍건호;김원기
    • Journal of the Earthquake Engineering Society of Korea
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    • v.6 no.6
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    • pp.65-72
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    • 2002
  • This study estimates steel house shear wall's seismic performance depending on trend of seismic design. As a result at cyclic-test, the capability of energy dissipation about X1SPCH during this test is good enough. The capability of energy dissipation of X3SPCH and X4SPCH was better than that of X1SPCH. The X2SPCH which is similar to real X-braced shear wall has better seismic performance than shear wall braced with structural sheathing materials on pseudo-dynamic test.

Comparison of Lifting and Lowering Activity based on Biomechanical, Physiological, Psychophysical Criteria (들기 작업과 내리기 작업의 생체역학적, 생리학적, 정신물리학적 기준치에 의한 비교)

  • Kim, Hong-Ki
    • Journal of the Ergonomics Society of Korea
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    • v.29 no.1
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    • pp.145-153
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    • 2010
  • Activity of lifting has been a major issue in many research area related in manual materials handling tasks. However, the opposite activity of lifting, lowering, has received much less attention. It is known that 52% of all box-handling tasks were lowering in nature. The difference in stress between lifting and lowering activity is not well understood. A simple assumption that these two activities are very similar has been established and widely used. However, this simple assumption may be questionable. The objective of this study was to compare a lifting activity and a lowering activity based on the three different ergonomic approaches; (1) biomechanical, (2) physiological, (3) psychophysical approach. It was found that the stress of lowering activity was from 65% to 93%, from 87% to 97%, and from 87% to 96% according to the biomechanical, physiological, and psychophysical point of view, respectively. It is concluded from the result of this study that the stress of lowering activity is lower than that of the lifting activity. The maximum compressive force on the lumbro-sacral joint (L5/S1) was 158% and 108% respectively, for lifting and lowering activity of which the work load is the 58% of Action Limit. It is suggested that the NIOSH AL and RWL and biomechanical criteria should be reconsidered especially for the low frequency of lifting activities.

Microspore Division and Plant Regeneration from Shed Pollen Culture in Rice

  • Kim, Hyun-Soon;Kang, Hyeon-Jung;Lee, Young-Tae;Lee, Seung-Yeob;Nam, Jeong-Kwon;Kim, Tae-Soo;Rha, Eui-Shik;Jin, Il-Doo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.1
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    • pp.62-67
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    • 2002
  • An efficient system of rice microspore culture could contribute to the production of genetically modified rice. The microspores were isolated by mechanical or shed methods. The number of microspores per 100 anthers isolated at uninucleate stage was higher than (or similar to) those at binucleate stage in isolation method with pestle or spatular, but microspore divisions were not easily observed on both stages. On the other hand, pollen division in shed pollen culture was observed more frequently at uninuclear than at binuclear stage. Cold pretreatment at 1$0^{\circ}C$ for 10 days resulted in the best multicellular division to produce microcalli at 12.5% efficiency in shed microspores. Heat shock at 33$^{\circ}C$ for one hour before or after pollen shedding enhanced cell division and callus formation. Out of twelve green regenerants, two were haploids and ten were diploids based on the chromosome analysis of root tips. The size of stoma was 12$^{m}$ m in haploids and 15 ${\mu}{\textrm}{m}$ in diploids determined by scanning electron microscope (SEM).

Pollution of Heavy Metals in Paddy Soils Around the Downstream Area of Abandoned Metal Mine and Efficiency of Reversed Soil Method as Its Remediation (폐금속광산 하부 농경지 토양의 중금속오염과 그 복구방법으로서 반전객토의 효율성)

  • Na, Choon-Ki;Lee, Mu-Seong;Chung, Jae-Il
    • Economic and Environmental Geology
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    • v.30 no.2
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    • pp.123-135
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    • 1997
  • In order to investigate the dispersion patterns and contamination level of heavy metals in the soil-ecosystem and to evaluate the efficiency of soil remediation by reversed soil method, soils and plants were collected from the Dongjin Au-Ag-Cu mine area and analysed for heavy metals. The dispersion patterns of heavy metals in soils and plants show that heavy metal pollutions caused by waste rump around Dongjin mine are mainly found in the vicinity of the waste rump and in the southward slanting of mine. Toxic metallic pollutants from the mine influence heavy metal contents in paddy soils in downstream area, and may be a potential sources of heavy metal pollution on crop plants. Soil samples collected from the remediated rice farming field by reversed method show similar levels of heavy metal content to those from the polluted rice farming field, but topsoil enrichment of heavy metals are not found. Heavy metal contents of the rice plants collected from remediated rice farming field are significantly lower than those from polluted rice farming field, and it suggests that the reversed soil method is effective for the reduction of bioavailability of heavy metals.

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Comparing Microscale Behaviors of Block Copolymer with Polymer Blend Thin Films under Electric Fields (전기장 하에서의 블록 공중합체와 고분자 블렌드의 미세 구조 변화 거동 비교)

  • Bae, Joonwon
    • Applied Chemistry for Engineering
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    • v.29 no.4
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    • pp.395-398
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    • 2018
  • In this work, profound microscale behaviors of block copolymer and polymer blend under electric field were investigated using microscopic methods and compared systematically. To this end, both the block copolymer and blend containing polyacrylonitrile (PAN) and poly(methyl methacrylate) (PMMA) were introduced. The two polymers have a similar dielectric constant. Under an identical experimental condition such as temperature, film thickness, field intensity, and exposure time, the polymer blend responded more sensitively than the block copolymer. The presence of covalent bond suppressed the mobility of constituents in block copolymer. This study will be essential for future research activities regarding behaviors of polymeric materials under external fields.

Nail Withdrawal Resistance and Embedding Strength of Structural Wood Panels with Water Absorption (수분의 흡수에 따른 구조용 목질판넬의 인발 및 함입성능)

  • 오세창
    • Journal of the Korea Furniture Society
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    • v.10 no.2
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    • pp.1-8
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    • 1999
  • The use of structural wood based panels like plywood, OSB in many applications involves the use of nails, and data on the performance of panel-nail connection are needed for design and comparative purpose. This study was carried out to develop basic information on nail performance(withdrawal, embedding, nailhead push through) with water absorption in plywood and OSB. In withdrawal resistance test, initial stiffness of plywood performed better than OSB in 24hr soaked condition. The soaked condition reduced average nail withdrawal resistance value about 46% (12d mail), 53%(8d nail) in OSB, 17%(12d mail), 24%(8d mail) in plywood. The nail embedding strength of plywood also performed better than that of OSB and retention value(wet strength/dry strength) yielded similar in each nail types. The nail head push through capacity of OSB was higher than that of plywood in dry condition, but equal or lower in soaked condition. Therefore, critical consideration about structural panels is required in moist applications. The average values of nail withdrawal, nail embedding strength and nail head push through resistance of tested materials meet the minimum requirement of various specification and standards.

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Control Efficacy of Milk Concentration Against Powdery Mildew of Strawberry

  • Nam, Myeong-Hyeon;Lee, Won-Keun;Lee, Sok-Su;Kim, Nam-Gyu;Kim, Hong-Gi
    • The Plant Pathology Journal
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    • v.21 no.3
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    • pp.270-274
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    • 2005
  • The aim of this study was to determine the effect of milk as one of the environmental friendly materials that substitute chemical fungicides for control powdery mildew (Sphaerotheca aphanis) of strawberries $(Fragaria{\times}ananassa\;Duch.)$. 'Maehyang' and 'Akihime' varieties planted in greenhouses were evaluated for the control of powdery mildew. Applications of $5\%,\;10\%\;and\;20\%$ milk had much better effects on controlling powdery mildew. In particular, $10\%$ milk showed a higher efficacy than other concentrations applied onto straw­berry in greenhouse experiments. Foliar spray application of $10\%$ milk was effective for powdery mildew, whereas drench application was not. Also, foliar spray of $10\%$ milk was able to accelerate more firmness and calcium contents of strawberry fruits than the non-treated. The $10\%$ milk applied with fertilizer salts $(Ca(H_2PO_4)_2-H_2O\;and KH_2PO_4)$ showed there was a similar efficacy to $10\%$ milk alone in greenhouse experiments. White crystals and cracks on strawberry fruits appeared by $20\%$ milk. This result indicated that $10\%$ milk was a useful substitute for fungicides to control powdery mildew of strawberry.

Damage Behavior of Elbow Pipe with Inner or Outer Local Wall Thinning under Internal Pressure (내압을 받는 내/외부 국부 감육 곡관의 파손거동)

  • Kim, Soo-Young;Nam, Ki-Woo
    • Journal of Power System Engineering
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    • v.18 no.5
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    • pp.66-73
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    • 2014
  • This study was considered to occur the local wall thinning at elbow which is flowing the steam and high-pressure water of high-temperature. The angle of elbow is ${\Theta}=45^{\circ}$ and $67.545^{\circ}$. The damage behaviors of inner or outer wall thinning elbow under internal pressure were calculated by FEA(finite element analysis). We compared the simulated results by FEA with experimental data. The FEA results are as follows: In the FEA results of three types of wall thinning ratio, the circumferential and longitudinal stresses show the similar values regardless of the angle of elbow, respectively. The circumferential strain was greater at elbow of small angle, but the longitudinal strain was nearly same. The FEM stress of outer wall thinning elbow was slightly higher than that of the inner wall thinning elbow, and strain was also slightly higher. In the experiments, the circumferential strain was increased with the increase in the internal pressure, and increased rapidly on about 0.2% of strain. The longitudinal strain was small. The strain at break was much smaller than 0.2%. In the relation between pressure and eroded ratio, the criteria that can be used safely under operating pressure and design pressure were obtained. The results of FEA were in relatively good agreement with those of the experiment.

Organ preservation with neoadjuvant chemoradiation in patients with orbit invasive sinonasal cancer otherwise requiring exenteration

  • Amsbaugh, Mark J.;Yusuf, Mehran;Silverman, Craig;Bumpous, Jeffrey;Perez, Cesar A.;Potts, Keven;Tennant, Paul;Redman, Rebecca;Dunlap, Neal
    • Radiation Oncology Journal
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    • v.34 no.3
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    • pp.209-215
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    • 2016
  • Purpose: We sought to determine if organ preservation (OP) with neoadjuvant chemoradiation (CRT) was feasible in patients with sinonasal cancer determined to require exenteration. Materials and Methods: Twenty patients were determined to require exenteration for definitive treatment from 2005 to 2014. Fourteen patients underwent OP and 6 patients received exenteration with adjuvant CRT. Exenteration free survival (EFS), locoregional control (LRC), progression-free survival (PFS), and overall survival (OS) were estimated. Results: Five patients (36%) receiving OP had complete disease response at time of surgery. With a median follow-up of 18.8 months, EFS was 62% at 2 years for patients undergoing OP. At 2 years, there were no significant differences in LRC, PFS or OS (all all p > 0.050) between the groups. Less grade 3 or greater toxicity was seen in patients undergoing OP (p = 0.003). Visual function was preserved in all patients undergoing OP. Conclusion: For patients with sinonasal cancer, OP may avoid exenteration, offering similar disease control and improved toxicity.