• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.1
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• pp.57-63
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• 2021

In this study, isoscoparin was selected as an indicator component to develop Silene seoulensis extract as a functional cosmetic material, and we developed an analysis method using high performance liquid chromatography (HPLC) for quality control. HPLC was performed on a Unison US-C18 with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methanol at a flow rate of 1.0 mL/min at 35 ℃, and the detection wavelength was 330 nm. The HPLC method was performed in accordance with the international conference on harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.02 and 0.07 mg/mL respectively. Calibration curves showed good linearity (R2 > 0.99988), and the precision of analysis was satisfied (less than 0.46%). In addition, the recovery rate was in the range of 99.10 to 101.61%, it was shown to be accurate. This result indicated that the established HPLC method is very useful for the determination of marker compounds in Silene seoulensis extracts.

• Korean Journal of Environment and Ecology
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• v.25 no.3
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• pp.267-283
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• 2011

Endangered species designated by The Ministry of Environment in region of Naejangsan National Park were found Lycoris chinensis var. sinuolata K.H.Tae & S.T.Ko, Iris koreana Nakai, Cymbidium macrorrhizum Lindl and Vexillabium yakushimensis (Yamam.) F.Maek.(4 taxa). The floristic special plants were recorded to a total of 159 taxa; that is, class I species (91 taxa) were containing as Chloranthus fortunei (A.Gray) Solms, Vicia anguste-pinnata Nakai and Euscaphis japonica (Thunb.) Kanitz, etc, class II species (17 taxa) were Pseudostellaria coreana (Nakai) Ohwi, Dryopteris expansa (C.Presl) Fraser-Jenkins et Jermy and Gymnocarpium dryopteris (L.) Newman, etc, class III species (32 taxa) were containing; Cirsium setidens (Dunn) Nakai, Parasenecio pseudotaimingasa (Nakai) B.U.Oh and Saussurea eriophylla Nakai, etc, class IV species (9 taxa) were Deutzia paniculata Nakai, Carex ligulata Nees and Thuja orientalis L., etc, and class V species (10 taxon) were Orobanche filicicola Nakai, Lycoris chinensis var. sinuolata K.H.Tae & S.T.Ko and Lycoris sanguinea var. koreana (Nakai) T.Koyama, etc. Endemic species of korea were identified 40 taxa such as Broussonetia kazinoki var. humilis Uyeki, Pseudostellaria coreana (Nakai) Ohwi and Silene seoulensis Nakai, etc. Plant species designated as Natural Monument were 2 species that Daphniphyllum macropodum forest(No. 91) and Torreya nucifera forest(No. 153) receive protection. The naturalized plants were identified 30 taxa; Fallopia dumetorum (L.) Holub, Persicaria orientalis (L.) Spach and Rumex crispus L., etc. Among them the ecosystem disturbance wild plants was not discovered.

• Korean Journal of Environment and Ecology
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• v.25 no.4
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• pp.447-469
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• 2011

The flora of Bongamsa (Temple) area, Mungyeong were listed 552 taxa; 99 families, 303 genera, 468 species, 1 subspecies, 70 varieties and 13 forms. Based on the rare plants (IUCN), total 12 taxa; Crypsinus hastatus (Polypodiaceae), Eranthis stellata (Ranunculaceae), Cnidium tachiroei (Umbelliferae), Monotropa hypopithys (Pyrolaceae), Rhododendron micranthum (Ericaceae), Salvia chanroenica (Labiatae), Gastrodia elata (Orchidaceae), etc. Based on the endemic plants, total 11 taxa; Asarum chungbuensis, Aconitum pseudolaeve, Corydalis maculata, Lonicera subsessilis, Aster koraiensis, Saussurea seoulensis, etc. Based on the specific plants by floral region were total 48 taxa (8.7% of all 552 taxa of vascular plants); 2 taxa in class V, 5 taxa (Cimicifuga heracleifolia, Wistaria floribunda, Elsholtzia angustifolia, etc.) in class IV, 10 taxa (Betula davurica, Spiraea chinensis, Acer barbinerve, Vaccinium koreanum, Syringa reticulata var. mandshurica, etc.) in class III, 7 taxa (Potentilla dickinsii, Viola orientalis, Ostericum melanotilingia, Melica nutans, Veratrum parulum, etc.) in class II, 24 taxa (Camptosorus sibiricus, Cephalotaxus koreana, Betula schmidtii, Ilex macropoda, Tilia amurensis, Triadenum japonica, Lobelia sessilifolia, etc.) in class I. Based on the naturalized plants, total 21 taxa (Silene armeria, Oenothera lamarckiana, Symphytum officinale, Erechtites hieracifolia, etc.) and naturalization rate was 3.8% of all 552 taxa of vascular plants. The flora of importance in the Bongamsa, rare plants were Crypsinus hastatus, Eranthis stellata, Gastrodia elata, Monotropa uniflora, Monotropa hypopithys, Rhododendron micranthum and Cnidium tachiroei, Iris ensata var. spontanea in intermediate marsh and alien species classify into planted species of Bongamsa, area for investigation of afforestation and artificial forests, introduced of naturalized plants.