• 한국약용작물학회지
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• 제18권1호
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• pp.15-21
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• 2010

This study was conducted to investigate the effects of Lycii fructus and Astragalus membranaceus mixed extracts on immunomodulators and prevention in a streptozotocin-induced diabetes rat model. A total of 28 male rats were divided into four dietary groups and fed a commercial diet (A), commercial diet plus induced diabetes by a streptozotocin (STZ) injection (B), induced diabetes by STZ plus medicinal crop extracts(I&$H^{(R)}$) diet (C), and medicinal crop extracts (I&$H^{(R)}$) diet (D). Immunoblotting analyses revealed cytokine expression, and ELISA analyses revealed immunoglobulin E and nitric oxide production. As a results, the tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and inducible nitric oxide synthase (iNOS) as a inflammatory cytokine were decreased. Interleukin-6 (IL-6) and signal transducer and activation of transcription 3 (STAT3) cytokine related in diabetes expression through JAK/STAT3 pathway were also decreased. Furthermore, immunoglobulin E and nitric oxide production were decreased in the serum and lens, respectively. These results suggest that Lycii fructus and Astragalus membranaceus mixed extracts provide positive effects on immunomodulators and prevention in diabetes and eye disease complications.

• 한국전자파학회논문지
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• 제23권11호
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• pp.1297-1306
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• 2012

본 논문에서는 77 GHz 차량용 레이더 시스템에 필요한 레이더 송신기를 설계하였다. 130 nm RF CMOS 공정을 이용하여 설계한 13 GHz 주파수 합성기로 6 체배기를 내장한 상용의 화합물 전력 증폭기를 구동하여 77 GHz 송신 신호를 발생시켰다. 13 GHz 주파수 합성기는 6 체배용 전력 증폭기를 구동하기 위해 4 dBm 출력을 내는 주입 잠금 버퍼를 내장하고 있다. 제작한 77 GHz 레이더 송신기 모듈은 주파수 조정 범위 내에서 출력 전력이 최소 13.99 dBm이고, 중심 주파수 대비 기준 스퍼의 크기는 -36.45 dBc이다. 또한, 76.5 GHz 중심 주파수의 1 MHz 오프셋에서 -81 dBc/Hz의 위상 잡음 특성을 보인다.

• 대한기계학회논문집B
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• 제28권11호
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• pp.1368-1375
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• 2004

Measurements of OH chemiluminescence in an atmospheric pressure, laboratory-scale dump combustor at equivalence ratios ranging from 0.63 to 0.89 were reported. The signal from the first electronically excited state of OH to ground state was detected through a band-pass filter with an ICCD. The objectives of this study are two: One is to see the effects of equivalence ratio on global heat release rate and local Rayleigh index distribution. To get the local Rayleigh index distribution, the line-of-sight images were inverted by tomographic method, such as Abel do-convolution. Another aim is to investigate the validity of using OH chemiluminescence acquired with an ICCD as a qualitative measure of local heat release. For constant inlet velocity and temperature, the overall intensities of OH emission acquired at different equivalence ratio showed periodic and higher value at high equivalence ratio. OH intensity averaged over one period of pressure increased exponentially with equivalence ratio. Local Rayleigh index distribution clearly showed the region of amplifying or damping the combustion instability as equivalence ratio increased. It could provide an information/insights on active control such as secondary fuel injection. Finally, local heat release rate derived from reconstructed OH images were presented fur typical locations.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권2호
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• pp.231-238
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• 2011

Attacin is an insect antibacterial protein that plays an important role in immune response to injury and infection. In this report, we have isolated and characterized of cDNA encoding for the attacin from the immunized larvae of swallowtail butterfly, $Papilio$ $xuthus$. A full length cDNA of $P.$ $xuthus$ attacin was obtained by employing annealing control primer (ACP)-based differential display PCR and 5' RACE. The complete $P.$ $xuthus$ attacin cDNA was comprised of 949 bp encoding a 250 amino acid precursor. It contains a putative 18 amino acid signal peptide sequence, a 42 amino acid propeptide sequence, and a 190 amino acid mature protein with a theoretical molecular mass of 19904.01 and a pI of 9.13. The putative mature protein of $P.$ $xuthus$ attacin showed 48-52% and 24-30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. Semiquantitive RT-PCR results revealed that the transcript of $P.$ $xuthus$ attacin gene was up-regulated at significant levels after injection with bacterial lipopolysaccharide (LPS). We sub-cloned cDNA fragment encoding mature $P.$ $xuthus$ attacin into the expression vector, highly expressed in $E.$ $coli$ BL21 cells, and its antibacterial activity was analyzed. Recombinant $P.$ $xuthus$ attacin evidenced considerably antibacterial activity against Gram-negative bacteria, $E.$ $coli$ ML 35 and $Klebsiella$ $pneumonia$.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.1
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• pp.141-144
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• 2006

The deviations in the injection orbital parameters, resulting from launcher dispersions, need to be estimated and used for autonomous satellite operations. For the proposed small satellite mission of the university there will be two GPS receivers onboard the satellite to provide the instantaneous orbital state to the onboard data handling system. In order to meet the power requirements, the satellite will be sun-tracking whenever there is no imaging operation. For imaging activities, the satellite will be maneuvered to nadir-pointing mode. Due to such different modes of orientation the geometry for the GPS receivers will not be favorable at all times and there will be instances of poor geometry resulting in no output from the GPS receivers. Onboard the satellite, the orbital information should be continuously available for autonomous switching on/off of various subsystems. The paper presents the strategies to make use of small arcs of data from GPS receivers to compute the mean orbital parameters and use the updated orbital parameters to calculate the position and velocity whenever the same is not available from GPS receiver. Thus the navigation message from the GPS receiver, namely the position vector in Earth-Centered-Earth-Fixed (ECEF) frame, is used as measurements. As for estimation, two techniques - (1) batch least squares method, and (2) Kalman Filter method are used for orbit estimation (in real time). The performance of the onboard orbit estimation has been assessed based on hardware based multi-channel GPS Signal simulator. The results indicate good converge even with short arcs of data as the GPS navigation data are generally very accurate and the data rate is also fast (typically 1Hz).

• Annals of Clinical Neurophysiology
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• 제2권1호
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• pp.8-12
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• 2000

Purpose : The rapid development and wide popularity of Digital EEG(DEEG) is due to its convenience, accuracy and applicability for quantitative analysis. These advantages of DEEG make one hesitate to use analog EEG(AEEG). To assess the advantage of DEEG system utilizing AEEG(DAEEG) over conventional AEEG and the clinical applicability, a DAEEG system was developed and applied to animal model Methods : Sprague-Dawley rat as status epilepticus model were used for collecting the EEG data. After four epidural electrodes were inserted and connected to 8-channel analog EEG(Nihon-Kohden, Japan), continous. EEG monitoring via computer screen was done from two rats simultaneously. EEG signals through analog amplifier and filters were digitized at digital signal processor and stored in Windows-based pentium personal computer. Digital data were sampled at a rate of 200 Hz and 12 bit of resolution. Acquisition software was able to carry out 'real-time view, sensitivity control and event marking' during continuous EEG monitoring. Digital data were stored on hard disk and hacked-up on CD-ROM for off-line review. Review system consisted of off-line review, saving and printing out interesting segment and annotation function. Results: This DAEEG system could utilize most major functions of DEEG sufficiently while making a use of an AEEG. It was easy to monitor continuously compared to Conventional AEEG and to control sensitivity during ictal period. Marking the event such as a clinical seizure or drug injection was less favorable than AEEG due to slowed processing speed of digital processor and central processing unit. Reviewing EEG data was convenient, but paging speed was slow. Storage and management of data was handy and economical. Conclusion : Relatively simple digital EEG system utilizing AEEG can be set-up at n laboratory level. It may be possible to make an application for clinical purposes.

• The Korean Journal of Physiology and Pharmacology
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• 제5권1호
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• pp.1-8
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• 2001

Hyperpolarization of arterial smooth muscle by acetylcholine is considered to be produced by the release of an unidentified chemical substance, an endothelium-derived hyperpolarizing factor (EDHF). Several chemicals have been proposed as the candidate for EDHF. However, none of them fulfil completely the nature and property of EDHF. Ultrastructural observation with electron microscope reveals that in some arteries, gap junctions are formed between endothelial and smooth muscle cells. In small arterioles, injection of gap junction permeable dyes into an endothelial cell results in a distribution of the dye to surrounding cells including smooth muscle cells. These observations allow the speculation that myoendothelial gap junctions may have a functional significance. Simultaneous measurement of the electrical responses in both endothelial and smooth muscle cells using the double patch clamp method demonstrates that these two cell types are indeed electrically coupled, indicating that they behave as a functional syncytium. The EDHF-induced hyperpolarization is produced by an activation of $Ca^{2+}-sensitive\;K^+-channels$ that are inhibited by charybdotoxin and apamin. Agonists that release EDHF increase $[Ca^{2+}]_i$ in endothelial cells but not in smooth muscle cells. Inhibition of gap junctions with chemical agents abolishes the agonist-induced hyperpolarization in smooth muscle cells but not in endothelial cells. All these observations can be explained if EDHF is an electrotonic signal propagating from endothelium to smooth muscle cells through gap junctions.

• Journal of Power Electronics
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• 제18권1호
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• pp.212-224
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• 2018

For maintaining a reliable and secure power system, this paper describes the design and implement of a single-phase grid-connected inverter with an enhanced phase-locked loop (PLL) and excellent power control performance. For designing the enhanced PLL and power regulator, a full-bridge voltage-controlled inverter (VCI) is investigated. When the grid frequency deviates from its reference values, the output frequency of the VCI is unstable with an oscillation of 2 doubling harmonics. The reason for this oscillation is analyzed mathematically. This oscillation leads to an injection of harmonics into the grid and even causes an output active power oscillation of the VCI. For eliminating the oscillation caused by a PLL, an oscillation compensation method is proposed. With the proposed method, the VCI maintains the original PLL control characteristics and improves the PLL robustness under grid frequency deviations. On the basis of the above analysis, a power regulator with the primary frequency and voltage modulation characteristics is analyzed and designed. Meanwhile, a small-signal model of the power loops is established to determine the control parameters. The VCI can accurately output target power and has primary frequency and voltage modulation characteristics that can provide active and reactive power compensation to the grid. Finally, simulation and experimental results are given to verify the idea.

• 한국정보통신학회논문지
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• 제25권7호
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• pp.955-961
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• 2021

현대전에서는 전파를 이용하여 적의 정보를 알아내거나, 적이 탐지하려는 아군 정보를 보호하는 임무를 수행하는 전자전 분야의 중요성이 증가하고 있다. 전자전 분야의 대표적인 전자공격 방법 중 하나인 레이다 재밍기법은 적 레이다를 교란 및 기만하여 아군의 위치 정보가 노출되는 것을 방지한다. 레이다 재밍기법 중 하나인 속도기만 재밍기법은 도플러효과를 이용하여 표적의 속도와 위치를 추적하는 펄스 도플러 레이다를 대상으로 사용된다. 속도기만 재밍기법은 DRFM (Digital Radio Frequency Memory)을 이용하여 수신신호를 주파수 변조해서 송신하는 방법으로 구현할 수 있다. 본 논문은 위상 샘플방식 DRFM을 이용하여 속도기만 재밍기법 중 하나인 VGPO/VGPI 재밍기법을 구현하는 방안을 기술하고, 제작한 보드를 통해서 주입신호 환경 하에서 VGPO/VGPI 재밍기법의 동작을 검증하였다.

• ALGAE
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• 제37권1호
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• pp.75-84
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• 2022

Intercellular nutrient and signal transduction are essential to sustaining multicellular organisms and maximizing the benefits of multicellularity. It has long been believed that red algal intercellular transport of macromolecules is prevented by the protein-rich pit plug within pit-connections, the only physical connection between cells. Fluorescein isothiocyanate-dextran and recombinant green fluorescence protein (rGFP) of various molecular sizes were injected into vegetative cells of Griffithsia monilis using a micromanipulator, and intercellular transport of the fluorescent probes was examined. Pit-connections were found to provide intercellular transport of tracers at rates comparable to plasmodesmata in other organisms. The time necessary for the transport to an adjacent cell was dependent on the molecular size and the direction of the transport. Fluorescent dextran of 3 kDa was transported to adjacent cells in 1-2 h after injection and migrated to all cells of the filament within 24 h, but fluorescent dextran of 10-20 kDa took 24 h to transfer to neighboring cells. The migration occurred faster towards adjacent reproductive cells and to apical cells than basally. Fluorescent tracers above 40 kDa and rGFP was not transported to neighboring cells, but accumulated near the pit plug. Our results suggest that pit-connections are conduit for macromolecules between neighboring cells and that these size-specific conduits allow intercellular communication between the vegetative cells of red algae.