• 제목/요약/키워드: side effects of antibiotics

검색결과 63건 처리시간 0.032초

("황제내경소문(黃帝內經素問)" 중(中) 사혈(瀉血)에 관한 연구(硏究) (A Study on the Blood-Letting Therapy in Elementary Questions)

  • 이준근
    • 대한한의정보학회지
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    • 제14권1호
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    • pp.19-42
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    • 2008
  • Blood-Letting Therapy is a rational and ecological medical treatment by which we can heal most of the diseases by removing the static blood which precipitates in the blood vessel and blocks the flowing of blood. And the static blood is the generic term for the injurious, bad, dead and precipitated blood which is blocked the capillary vessel. The Yellow Emperor's Canon of Internal Medicine says that "the patient is treated with drugs internally and stone acupuncture externally. "In the old texts, the blood-letting therapy is mentioned as blood-letting, network vessel pricking, bloodletting, pricking, and arousing pulses etc and it is noted down as the method of network vessel pricking in 'On the Application of Needles' of Spiritual Pivot. Nine-pricking therapy, twelve-pricking therapy and five-pricking therapy are recorded in the methods of network vessel pricking and among them, the method of squeezing blood after pricking the affected part is explained as the network vessel pricking. There are four methods of network vessel pricking, pricking, picking, cluster needling and scatter-pricking and they are fluidly applied to the various symptoms of diseases. In 'On Discriminative Treating for Patients of Different Regions' in Elementary Questions, Ki-baek emphasizes "most of the local people, there are black in skin and loose in striate, and their diseases are mostly of carbuncle kind. It is suitable to treat the disease with stone therapy to prick with stone, so the stone therapy is transmitted from the east. "And in 'On the Corresponding Relation Between the Eum and Yang of Man and All Things' in Elementary Questions, when the Emperor asked Ki-Back, he answered "sthenia means the sthenia of evil, and deficiency means the deficiency of healthy energy. When the blood is sthenic, the evil should be discharged by pricking when out letting the blood; deficiency of vital energy is the asthenia of channels and network vessels, so the energy should drain from the channel which is not deficient, to replenish. "And in this case we can use the methods of 'Breaking out the static bloods', 'driving out the static bloods', blood-letting'. With this we can infer that the blood-letting therapy is made use of the important medical treatments from the ancient times. Especially in referring to the principles of treatment in The Yellow Emperor's Canon of Internal Medicine, it mostly alluded to acupuncture therapies and only eleven times to medicinal treatments. This is to verify that the blood-letting therapy formed the foundation of the medical art. In Dong's Therapy of Acupuncture-Moxibustion and Bloodletting, Dong Kyeong-Chang gave emphasis on the points that there must be extravasated bloods without exception in the serious illnesses which is old, unnatural, accompanying acute pains and so we can revive our body‘s sprit by circulating 'gi' and static blood piled up in the network vessel, regulating the weakness and strength, and controling the disharmony of the internal organs. The blood-letting therapy has effect on the orifice in emergency, such as fore draining, freeing network vessels, harmonizing gi and blood, relieving pain, dispersing swelling and concretion, sedation, resolving toxin as well as strengthening the heart, relieving itching. So it has distinguished effect on all kinds of medical treatment to the modern people. But by the change of social customs and the confucianism of confucius - it is widely spread on the period of North and South Dynasties, 'Wi' and 'Jin' in china and the period of the Three States in korea - The blood-letting therapy which was regarded as the most important medicinal treatment withered rapidly. And Confucius accentuated the importance of our body and all its members, loyalty and filial piety and banned any damage of our body under no circumstances. As a result of it, the therapy of blood-letting had a rapid decrease and barely kept itself in existence in both countries. What is worse, at the period of Japanese colonial rule of korea and our nation's founding of early stage, it has been withered by the high-handed policy to change Oriental Medicine into modern medical science. So the therapy of blood-letting barely kept itself in existence in some Buddhist temples. Another case, it has handed down as a old-fashioned quick fix in folk remedies. But all kinds of the contamination of heavy metals and the misuses of antibiotics are widely spread nowadays, which increased diseases of adult people and incurable diseases as modern society unavoidably made its way into a highly industrial society. To make us more miserable, the western medical science - the antibiotics and surgical operation medical science - already reveals itself into a limit. The necessity of a new medical science which can give a security to the patients who are suffering from the diseases of adult people and the incurable diseases is especially come into the force nowadays. In view of the results after bibliographically studying on the blood-letting Therapy in Elementary Questions of the Yellow Emperor's Canon of Internal Medicine, the blood-letting therapy has acted for the important Oriental medicinal science and has been clarified the prominent effects on the diseases of adult people and the incurable diseases. So it is regarded as an appropriate thing that we lay out a determined theory of the blood-letting therapy and of course prevent the unwanted side effects from inappropriate medicinal treatments, and make full use of clinic by elevating the curative value and that we win back our self-respect of medical treatment which is dominated from the western medical science and ultimately contribute to national medical welfare.

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Lipomyces starkeyi KSM 22 Glucanhydrolase 용액의 치은 섬유아세포에 대한 영향 (Effects of Lipomyces starkeyi KSM 22 Glucanhydrolase on human gingival fibroblasts)

  • 윤현정;정현주;김옥수;김도만
    • Journal of Periodontal and Implant Science
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    • 제32권3호
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    • pp.665-683
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    • 2002
  • A novel glucanhydrolase from a mutant of Lipomyces starkeyi KSM 22 has additional amylase activity besides mutanolytic activity and has been suggested as promising anti-plaque agent. It has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi KSM 22 glucanhydrolase are desirable for its application as a dental plaque control agent. In human experimental gingivitis model and 6 month clinical trial, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effect was negligible. This study was aimed to evaluate the cytotoxic effect of Lipomyces starkeyi KSM 22 glucanhydrolase on human gingival fibroblasts. Primary culture of human gingival fibroblasts at the 4th to 6th passages were used. Glucanhydrolase solution was made from lyophilized glucanhydrolase powder from a mutant of Lipomyces stakeyi KSM 22 solved in PBS and added to DMEM medium to the final concentration of 0.5, 1, and 2 unit. Cells were exposed to glucanhydrolase solution or 0.1 % chlorhexidine and the cells cultured in DMEM with 10% FBS and 1% antibiotics as control. After exposure, the morphological change, cell attachment, and cell activity by MTT assay were evaluated in 0.5, 1.5, 3, 6, 24 hours after treatment. The cell proliferation and cell activity was also evaluated at 2 and 7 days after 1 minute exposure, twice a day. The cell morphology was similar between the Lipomyces smkeyi KSM 22 glucanhydrolase groups and control group during the incubation periods, while most fibroblasts remained as round cell regardless of incubation time in the chlorhexidine group. The numbers of the attached cells in the glucanhydrolase groups were comparable to that of control and significantly higher than the chlorhexidine group. The numbers of the proliferated cells in the glucanhydrolase groups at 7 days of incubation were comparable to the control group and higher than the chlorhexidine group. The cell activity in glucanhydrolase groups paralleled with the increased cell number by attachment and proliferation. According to these results, Lipomyces starkeyj KSM 22 glucanhydrolase has little harmful effect on attachment and proliferation of human gingival fibroblasts, in contrast to 0.1% chlorhexidine which was cytotoxic to human gingival fibroblasts. Therefore this glucanhydrolase preparation is considered as a safe and promising agent for new mouthwash formula in the near future.

Meat quality of pork loins from Hereford×Berkshire female and intact male pigs reared in an alternative production system

  • Robbins, Yvette;Park, Hyeon-Suk;Tennant, Travis;Hanson, Dana;Whitley, Niki;Min, Byungrok;Oh, Sang-Hyon
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권9호
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    • pp.1475-1481
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    • 2019
  • Objective: The objective of the present study was to investigate pork quality from Hereford${\times}$Berkshire female and intact male pigs reared outdoors in an alternative production system. Methods: Berkshire purebred sows were artificially inseminated, once in the fall and again in the spring of the following year, with semen from Hereford boars and managed free of antibiotics in an outdoor hoop structure until the last month of pregnancy, after which they were moved to a pasture-based unit of 0.8 hectares with individual lots with a farrowing hut, shade, and water ad libitum. Piglets were weaned at 4 weeks of age and housed in a deep-bedded hoop structure, grouped by sex. Animals were harvested at market weight of 125 kg, approximately 200 days of age. Hot carcass weight was collected at the time of the harvest. After 24 hours of refrigeration, carcass characteristics were measured. Longissimus dorsi samples collected from the right side loin. Loins were cut into 2.54-cm thick chops and were used to measure marbling score, color score, drip loss, and ultimate pH. Sensory panel tests were conducted as well at North Carolina State University. For pork characteristics and sensory panel data, trial and sex were included in the statistical model as fixed effects. Hot carcass weight was included in the model as a covariate for backfat thickness. Results: Neither the subjective nor the objective color scores displayed any differences between the boars and the gilts. No difference was found for pH and marbling score between trials or sexes. Gilts had a thicker backfat measurement at the last lumbar and a narrower longissimus muscle area measurement when compared to the boars. The only difference in the sensory characteristics was found between the trials for texture and moisture scores. Conclusion: Consumers were not able to detect boar taint under the condition of this study, which is that the intact males were reared outdoors. Additional trials would be necessary; however, based on the results of the present study, outdoor rearing can be suggested as a solution to the issue of boar taint.

식물근권에서 분리한 세균의 어류질병세균에 대한 항균활성 효과 (Antibacterial Effect of Bacteria Isolated from the Plant Rhizosphere against Pathogenic Bacteria of Fish)

  • 정지운;박소현;김동휘;전용철;허문수
    • 생명과학회지
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    • 제24권7호
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    • pp.757-761
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    • 2014
  • 천연 항균물질의 연구는 대부분 식품소재, 특히 약용식물을 대상으로 하여 많은 연구가 진행되어 왔다. 그러나 식품소재의 경우 대량의 추출물 등을 제조하기 위해 많은 양의 재료가 필요하며, 이로 인한 비용 문제가 발생하게 된다. 미생물은 적절한 환경 하에서 대량 배양이 용이하기 때문에 천연항생제로 이용하기 위해 지속적인 연구가 이루어지고 있다. 본 연구에서는 식물병 억제에 효과가 있는 식물근권미생물을 이용하여 어류질병세균 4종을 대상으로 항균활성을 확인하였다. 이 중 항균활성을 나타낸 균 3종을 선발하여 배양액, 상층액, 균체 현탁액으로 나누어 어느 부위에서 항균효과를 나타내는지 탐색하고, 16S rDNA 염기서열 분석을 통해 균을 동정하였다. 12종의 식물근권세균 중 BRH433-2, THJ609-3, TRH415-2 3종에서 항균활성이 나타나는 것을 확인하였다. 세 균주의 배양액, 상층액, 균체 현탁액 중에서는 균체 현탁액이 가장 강한 항균활성을 나타내는 것을 알 수 있었다. 세 균주의 16S rDNA 염기서열 분석 결과, BRH433-2는 Burkholderia gladioli와 99.5%, B. plantarii 98.9%, B. ubonensis 98.5%, B. pyrrocinia 98.3%, B. glumae 98%로 나타났다. THJ609-3은 Pseudomonas baetica와 97.7%, P. moorei와 P. plecoglossicida에 대해서는 각각 97.6%, 97.5%의 상동성이, TRH415-2는 P. koreensis, P. baetica와 98.4%, P. moraviensis 98.3%의 염기서열 유사도를 나타났다. 따라서 3종의 식물근권세균은 충분한 안정성 및 안전성 실험을 실행한 후, 양식산업에 적용하여 항생제 대체 물질로서의 이용가치가 있다고 생각된다.

아토피피부염을 동반한 성인 삼출성 중이염 치험례 (A Case of Adult Otitis Media with Effusion Accompanied by Atopic Dermatitis)

  • 송지훈;우배언;김종한;정민영;최정화;박수연
    • 한방안이비인후피부과학회지
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    • 제35권2호
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    • pp.95-104
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    • 2022
  • Objectives : This study is to report a case of otitis media with effusion accompanied by atopic dermatitis improved by Korean medicine monotherapy. Methods : A 31-year-old woman patient visited our clinic for aural fullness, hearing loss, and tinnitus of the left ear on August 14th, 2021. She also had abdominal pain, diarrhea, and general weakness because of side effects of antibiotics. Besides, she had nasal congestion, rhinorrhea, and dry skin. Through otoscopy, her tympanum was fully filled with yellow effusion. She only took Korean medicine therapy including acupuncture and herbal medicine weekly. The treatment continued for about 4 months(total 14 visits) until December 4th, 2021. Otoscopy, numerical rating scale(NRS), scoring atopic dermatitis(SCORAD) index were used to assess the symptoms. Results : On August 30, 2021, her intestinal symptoms improved. The left tympanum was recovered to normal condition as the treatment continued. The effusion disappeared on October 16th, 2021. On November 6th, 2021, peritympanic blood congestion also almost alleviated. Subjective symptoms including aural fullness, hearing loss, and tinnitus disappeared on October 16, 2021. Nasal symptoms and dry skin also improved. The SCORAD index dropped from 15 to 4. Conclusions : These results suggests that otitis media with effusion can be sufficiently managed by Korean medicine therapy. Further, active application of Korean medicine therapy to common otolaryngological diseases is necessary.

동위원소희석 액체크로마토그래피-질량분석법을 이용한 육류 중 클로람페니콜의 정밀한 정량 분석에 관한 연구 (Accurate determination of chloramphenicol in meat by isotope dilution liquid chromatography mass spectrometry (ID-LC/MS))

  • 송혜민;김병주;정혁;안성희
    • 분석과학
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    • 제23권6호
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    • pp.524-530
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    • 2010
  • 클로람페니콜은 미생물과 박테리아들의 활동을 억제시킴으로써 동물 치료에 효과적인 축산 항생제 중의 하나이다. 그러나 사람에게 재생 불량성 빈혈과 같은 치명적인 부작용이 발생하여 식용으로 사육하는 동물들에 대한 클로람페니콜의 사용을 유럽을 포함한 많은 나라에서 금지하였다. 본 연구에서는 돼지고기 시료 중 클로람페니콜을 분석하기 위하여 동위원소희석 액체크로마토그래피-질량분석법(IDLC/MS/MS)을 이용하여 정확하고 정밀한 정량분석을 하였다. 돼지고기 시료중의 클로람페니콜은 에틸아세테이트로 추출하였으며, 회수율을 높이기 위하여 고체상 추출법(Solid Phase Extraction)을 이용하였다. 액체크로마토그래피/질량분석기는 전기 분무 이온화 장치(Electrospray Ionization, ESI)를 장착하여 음이 온 모드로 선택반응분석법(Selected Reaction Monitoring mode)에서 수행하였다. 클로람페니콜의 분자이온 $[M-H]^-$이온이 조각이온인 $[M-H-(HCOCl)]^-$로 가는 m/z 321 ${\rightarrow}$ 257와 동위원소의 m/z 326 ${\rightarrow}$ 262 채널을 선택하여 분석하였다. 클로람페니콜을 약 0.2, 1, 10, $25\;{\mu}g$/kg의 농도가 되도록 첨가한 돼지고기 시료로부터 제조값과 측정 분석값을 비교하여 검토한 결과, 측정값들은 불확도 범위 내에서 제조값과 일치함을 보였다. 또한 IRMM(Institute for Reference Materials and Measurement)의 클로람페니콜 분석용 돼지고기 시료인 인증 표준물질 BCR 445를 분석한 결과, 본 실험의 측정값은 불확도 범위 내에서 인증값과 일치함을 보였으며 불확도의 경우에는 IRMM이 제공한 값보다 훨씬 향상된 값을 보였다.

Doxorubicin과 Cyclophosphamide를 투여받는 유방암 환자에서 Pegfilgrastim과 Pegteograstim의 비용-효용 분석 (Cost-Utility Analysis of Pegfilgrastim and Pegteograstim in Patients with Breast Cancer using Doxorubicin and Cyclophosphamide)

  • 권수지;금민정;김재송;손은선;권경희
    • 병원약사회지
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    • 제35권4호
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    • pp.409-417
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    • 2018
  • Background : Febrile neutropenia (FN) is one of the side effects in the patients treated with chemotherapy, and the patients who have FN generally need immediate treatment with extended-spectrum antibiotics and hospitalization. Pegfilgrastim and pegteograstim, which are used for the prevention of FN as a granulocyte-colony stimulating factor (G-CSF), have been granted insurance coverage in the Republic of Korea for certain breast cancer patients using doxorubicin and cyclophosphamide (AC) from September 2016. Methods : The data of the patients with breast cancer using AC regimen and G-CSF were collected retrospectively. This study involves cost-utility analysis of pegfilgrastim and pegteograstim. In this study, we constructed a simple decision tree model for short-term observation and calculated quality-adjusted life year (QALY) and the direct medical costs from the medical provider's perspective. Results : From September 2016 to May 2017, 15 patients were treated with pegfilgrastim and 15 patients were treated with pegteograstim. As a result of dividing the average cost by QALY for each treatment group, it was observed that pegfilgrastim and pegteograstim were consumed 24,923,384 won and 22,808,336 won per 1QALY, respectively. Consequently, incremental cost effectiveness ratio (ICER) showed 2,115,048 won more per pegfilgrastim than pegteograstim per 1QALY, and the cost per 1QALY of both the drugs was lower than 30,500,000 won; the Koreans were willing to pay this amount. Conclusions : This study suggests that pegfilgrastim and pegteograstim can be used to improve the quality of life of breast cancer patients undergoing AC therapy. Among the two drugs, pegteograstim seems to be more cost-effective. However, since this study was conducted as a retrospective observation method on a small scale, it is associated with many limitations. Therefore, a long-term prospective cohort study is needed to supplement the present findings.

황련해독탕의 항균활성 및 표준화 연구 (Anti-bacterial Activity and Standardization on Hwangryunhaedok-tang)

  • 이대연;이영우;김완수;심선형;권필승;이인희
    • 대한임상검사과학회지
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    • 제51권2호
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    • pp.214-220
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    • 2019
  • 황련해독탕은 한방산업에서 염증관련 질환에 다빈도로 사용되는 처방이다. 본 연구의 목적은 전통적 한약인 황련해독탕의 항균활성을 확인하고 지표성분을 선정하는 것이다. 황련해독탕의 항균활성을 측정하기 위하여 디스크 확산법과 최소억제농도법을 사용하였다. 사용균주는 Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 23724였으며 대조군으로 oxacillin과 ciprofloxacin을 이용하여 황련해독탕의 항균활성을 확인하였다. 결과적으로 디스크 확산법에서는 S.aureus ATCC 25923에서 황련해독탕 20 mg과 10 mg은 $11.7{\pm}1.3mm$, $8.7{\pm}0.7mm$의 억제환을 형성하였으며 E.coli ATCC 23724에서는 황련해독탕 20 mg이 $8.0{\pm}0.0mm$의 억제환을 형성하였다. 최소억제농도는 S.aureus ATCC 25923가 31.25 mg/mL, E.coli ATCC 23724 에서는 125.0 mg/mL였다. 표준화를 위한 HPLC 정량분석 결과, 황련해독탕은 berberine을 16.55 mg/g 함유하고 있었으며 geniposide를 81.85 mg/g 함유하고 있었다. 천연물을 이용하여 항생제를 개발할 경우에는 내성세균을 억제하고 부작용을 줄일 수 있다는 장점이 있다. 이러한 점을 착안하여 지속적으로 연구를 진행한다면 한방산업과 더불어 여러 산업에서의 발전을 기대할 수 있을 것이다.

관절경적 활액막 절제술 후 후격막 삽입구를 통한 배액관의 후방 거치를 이용한 무릎의 화농성 관절염 치료 (Treatment of Pyogenic Arthritis of the Knee Using Drainage Tube Insertion in the Posterior Compartment through the Posterior Transeptal Portal after an Arthroscopic Synovectomy)

  • 김태호;유창은;신충식
    • 대한정형외과학회지
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    • 제54권3호
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    • pp.269-275
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    • 2019
  • 목적: 화농성 슬관절염의 관절경적 활액막 절제 및 후격막 절제 후 후내측 부위에 배액관 삽입 후 치료 효과를 분석하였다. 대상 및 방법: 2005년 6월부터 2016년 12월까지 화농성 관절염으로 진단된 56예(55명, 양측 1예)를 대상으로 하였다. 원인균이 동정된 환자는 14명(25.0%)이었다. 평균 추시 기간은 12.3개월이었고 평균 나이는 67.8세였다. 이 중 남성은 24명, 여성은 31명이었다. Kellgren-Lawrence 등급 I은 6예, II는 20예, III는 11예, IV는 18예였다. 수술방법은 총 6개의 삽입구를 통하여 변연 절제술을 시행하였고 후격막을 절제한 후 배액관을 후내측 삽입구에서 관절내 후외측까지 삽입하여 수술을 마무리하였다. 수술 후 기능적 평가로 Lysholm score를 사용하였고, 방사선학적 평가로 내원 당시와 최종 추시 시의 Kellgren-Lawrence 등급을 비교하였다. 결과: C-반응성 단백의 정상화 기간은 평균 59.8일(6-164일)이었다. 정맥 항생제는 평균 37.1일 동안 사용되었다. 기능평가를 통한 결과에서는 Lysholm score상 평균 64.5점(30-98점)이었다. 총 2예에서 재발하였는데 동일한 방법으로 한 차례 재수술하였으며 모든 환자에서 치료가 완료되었다. 수술 당시와 마지막 추시 시의 Kellgren-Lawrence 등급은 유의한 차이가 없었다(p>0.05). 결론: 화농성 슬관절염에서 관절경적 변연 절제술 후에 부가적으로 후격막을 부분 절제하고 배액관을 후방에 위치시키는 술식은 적절한 삼출물 배출을 유도하여 만족스러운 결과를 얻을 수 있어 고려해 볼만한 치료 방법 중의 하나로 생각된다.

테트라싸이크린 함유 calcium sulfate의 서방형 국소 약물 송달 효과에 대한 연구 (Slow-release local drug delivery effect of tetracycline loaded calcium sulfate)

  • 김성희;최성호;조규성;채중규;박광균;김종관
    • Journal of Periodontal and Implant Science
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    • 제27권4호
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    • pp.751-765
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    • 1997
  • 치주질환은 세균에 의한 감염성 질환으로, 기계적 치태제거에 의한 치료의 한계를 극복하기 위하여 항세균 제재를 통한 화학적 치태 및 세균제거가 필요하게 되었다. 전신적으로 항생제를 투여할 경우 유효농도의 유지를 위해 많은 양의 약물이 투여되어야 하고, 여러가지 부작용의 위험이 있으므로, 이러한 단점을 극복하기 위한 국소 약물 송달체계가 필요하게 되었다. 본 실험의 목적은 치주질환 치료에 가장 많이 쓰이는 tetracycline을 calcium sulfate와 혼합하여, calcium sulfate의 서방형 국소 약물 송달효과에 대해 알아보고자 함이다. Modified calcium sulfate paste 와 10% tetracycline을 혼합한 것을 실험 1군으로, calcium sulfate와 10% tetracycline을 혼합하여 완전 경화 시킨 것을 실험 2군으로, calcium sulfate와 10% tetracycline을 혼합하여 경화되기 전에 사용한 것을 실험 3군으로, tetracycline-ethylene vinyl acetate fiber를 실험 4군으로 하여 시간별 tetracycline 방출농도, 유효농도 지속시간, calcium sulfate의 흡수기간 등을 관찰하여 다음과 같은 결론을 얻었다. 1. 실험 1군은 실험 5주까지 유효농도($4{\mu}g/ml$)이상의 농도를 유지하였고, 실험 2군은 실험 9일까지 유지하였으며, 실험 3군은 실험 7일까지 유효농도이상의 농도를 유지하였고, 실험 4군은 실험 15일까지 유지하였다. 2. 실험 2군은 평균 11.8일에 완전 용해 되었고, 실험 3군은 14.8일에 완전용해되었다. 3. 실험 2군과 3군은 실험 1주까지 방출된 tetracycline의 농도에 유의차를 보이지 않았다(p<0.05). 이상의 결과에서 볼 때, calcium sulfate는 tetracycline과 혼합하였을 때 일정 기간 동안 충분한 양의 tetracycline을 방출시키고, $11{\sim}14$일 정도 후에는 완전히 녹아 없어지므로, 서방형 국소 약물 송달 제재로서 가능성이 있으리라 생각된다.

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