• 분석과학
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• 제23권6호
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• pp.524-530
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• 2010

클로람페니콜은 미생물과 박테리아들의 활동을 억제시킴으로써 동물 치료에 효과적인 축산 항생제 중의 하나이다. 그러나 사람에게 재생 불량성 빈혈과 같은 치명적인 부작용이 발생하여 식용으로 사육하는 동물들에 대한 클로람페니콜의 사용을 유럽을 포함한 많은 나라에서 금지하였다. 본 연구에서는 돼지고기 시료 중 클로람페니콜을 분석하기 위하여 동위원소희석 액체크로마토그래피-질량분석법(IDLC/MS/MS)을 이용하여 정확하고 정밀한 정량분석을 하였다. 돼지고기 시료중의 클로람페니콜은 에틸아세테이트로 추출하였으며, 회수율을 높이기 위하여 고체상 추출법(Solid Phase Extraction)을 이용하였다. 액체크로마토그래피/질량분석기는 전기 분무 이온화 장치(Electrospray Ionization, ESI)를 장착하여 음이 온 모드로 선택반응분석법(Selected Reaction Monitoring mode)에서 수행하였다. 클로람페니콜의 분자이온 $[M-H]^-$이온이 조각이온인 $[M-H-(HCOCl)]^-$로 가는 m/z 321 ${\rightarrow}$ 257와 동위원소의 m/z 326 ${\rightarrow}$ 262 채널을 선택하여 분석하였다. 클로람페니콜을 약 0.2, 1, 10, $25\;{\mu}g$/kg의 농도가 되도록 첨가한 돼지고기 시료로부터 제조값과 측정 분석값을 비교하여 검토한 결과, 측정값들은 불확도 범위 내에서 제조값과 일치함을 보였다. 또한 IRMM(Institute for Reference Materials and Measurement)의 클로람페니콜 분석용 돼지고기 시료인 인증 표준물질 BCR 445를 분석한 결과, 본 실험의 측정값은 불확도 범위 내에서 인증값과 일치함을 보였으며 불확도의 경우에는 IRMM이 제공한 값보다 훨씬 향상된 값을 보였다.

• 한국응용곤충학회지
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• 제22권2호
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• pp.147-159
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• 1983

In general, herbicides have been classified according to selectivity, mobility. time of application, methods of application, mode of action and chemical property and structure. However, there was no generally accepted classification system for practical use in the field. The primary processes affected by the majority of herbicides are the growth process through cell elongation and/or cell division, the photosynthetic process specifically the light reaction, the oxidative phosphorylation and the integrity of the membrane systems. The usual approach in the study of the mechanism by which herbicides kill or inhibit the growth of plants is to initially determine the morphological phototoxicity systems, The mechanism by which a herbicide kills a plant or suppresses its development is actually the resultant effect of primary and secondary(or side) effects. In most instances, the death of the plant is due to the secondary effects. To induce the desired response, a herbicide must be able to gain entry into the plants and once inside, to be transported within the plant to its site(s) of activity in concentrations great enough. Obstacles to the entry and movement of herbicides in plants are generally classified by leaf and soil obstacles, translocation obstacles and biochemical obstacles, and these obstacles are also strongly influenced by plant species and by environmental factors such as light, temperature, rainfall and relative humidity. And hence, in most instances, results obtained from laboratory or greenhous vary from those of field experiment. Author attempted to classify herbicides from the field experiment using the two-dimensional ordination analysis to obtain practical information for selecting effective herbicides or to choose effective herbicide combinations for increasing herbicidal efficacy or reducing the chemical cost. Based on this two-dimensional diagram, desired herbicides or combinations were selected and further investigated for the interaction effects whether these combinations are synergistic, additive or antagonistic. From the results, it was concluded that these new approach could possibly be give more comprehensive informations about effective use of herbicide than any other systems.

• 한국소음진동공학회논문집
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• 제13권10호
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• pp.798-804
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• 2003

One of the methods that are used to compare and verify the supporting performance of the spacer grids developed is the vibration characteristic test. A modal test in this paper is performed for a dummy rod 3,847 mm tall supported by eight New Doublet (ND) spacer grids. For the vibration test in air, nine accelerometers, one displacement sensor and one shaker are used for acquiring signals, and an I-DEAS TDAS software Is employed for analyzing the signals. Also, a finite element (FE) analysis is performed by a beam-spring simple model and a contact model simulating the contact phenomenon between the rod and the ND spring. And then, the results of the modal testing are compared with those of the FE analysis. The natural frequencies as well as the mode shapes obtained by the experiment have a greater similarity to the results by the contact model than the previous beam-spring model. In audition, for grasping whether or not the modal parameters are influenced by where shaking spot is, two kinds of tests are performed : one is for the shaker attached at the fourth span (center), the other is for the shaker at the fifth span that is one span nearer to the bottom of the rod. The latter shows higher MAC than the former Finally, the vibration displacements are measured in the range of 0.l12∼0.214 mm for the excitation force of 0.25∼0.75 N.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제48권8호
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• pp.431-439
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• 1999

Among the variable-speed AC motor drive systems, the static slip power recovery system has been widely adopted in large power drives because a high efficiency drive can be obtained by recovering the slip power to the AC line. Although many improvements have been made in this system, several problems also remain such as the need of transformer in inverter AC side, which results in limiting speed control range and increasing the losses, production of reactive power by the control of inverter firing angle, harmonics in line currents, and so on. This paper presents the novel high performance slip power recovery system using the boost converter and small size filter in the rotor circuit, which recovers slip power of a wound rotor induction machine to AC supply efficiently with the aid of the boost converter, in which most of the problems in conventional system can be solved. The speed can be controlled by the duty ratio of the converter switch, not by inverter firing angle. As a results, the proposed system can operate in high power factor and the harmonic currents caused by the inverter and rectifier can be considerably suppressed. The validity of the proposed system verified by demonstrating the good agreement in the simulation and experimental results.

• BMB Reports
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• 제40권5호
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• pp.649-655
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• 2007

The nsp14 protein is an exoribonuclease that is encoded by severe acute respiratory syndrome coronavirus (SARS-CoV). We have cloned and expressed the nsp14 protein in Escherichia coli, and characterized the nature and the role(s) of the metal ions in the reaction chemistry. The purified recombinant nsp14 protein digested a 5'-labeled RNA molecule, but failed to digest the RNA substrate that is modified with fluorescein group at the 3'-hydroxyl group, suggesting a 3'-to-5' exoribonuclease activity. The exoribonuclease activity requires $Mg^{2+}$ as a cofactor. Isothermal titration calorimetry (ITC) analysis indicated a two-metal binding mode for divalent cations by nsp14. Endogenous tryptophan fluorescence and circular dichroism (CD) spectra measurements showed that there was a structural change of nsp14 when binding with metal ions. We propose that the conformational change induced by metal ions may be a prerequisite for catalytic activity by correctly positioning the side chains of the residues located in the active site of the enzyme.

• 한국지반공학회논문집
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• 제20권8호
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• pp.135-145
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• 2004

본 연구에서는 화강풍화토 지반상 unpropped diaphragm wall의 거동을 연구하기 위하여 과재하중의 이격거리를 변화시키면서 원심모형실험을 수행하였다. 원심모형실험시 지반굴착은 흙과 동일한 밀도로 혼합된 zinc chloride 용액이 배수되도록 밸브를 조작하여 실시하였으며, 굴착에 따라 발생하는 지반의 변형과 벽체의 변위 및 휨모멘트를 측정하였다. 수치해석은 대부분의 지반공학 문제에 적용할 수 있는 FLAC 프로그램을 이용하였다. 수치해석에서 모형지반은 Mohr-Coulomb 모델, diaphragm wall은 탄성모델을 사용하여 2차원 평면변형률 조건으로 해석을 수행하였다. 모형실험 결과 파괴면의 직선적인 형태로 파괴면내의 배면측 지반은 벽체를 향하여 하향의 변위를 일으키면서 벽체의 회전에 의해 파괴되었으며, 파괴면의 각도는 67∼74$^{\circ}$정도로 이론적인 파괴면의 각도보다 크게 평가되었다. 실험 및 해석 결과 지반의 최대침하량이 발생하는 위치는 잘 일치하였으며, 깊이에 따른 벽체변위는 선형적인 관계를 나타내었다.

• 한국진공학회지
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• 제7권2호
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• pp.118-126
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• 1998

반도체 접촉구를 메우기 위하여 소오스의 직진성을 향상시키기 위한 연구를 수행하 였다. 이온화클러스터빔 증착법을 이용하는 동시에 셀의 구조를 개선하여 직진성 향상을 도 모하였다. 중성클러스터 만으로 구리를 증착할 경우 직진성은 매우 우수하였으나 소오스의 표면 이동이 적어 박막은 주상형으로 성장하며 측벽에의 증착은 거의 일어나지 않았으며 성 장에 따라 그림자효과로 인한 단차에서의 벽개가 관찰되었다. 그러나, 가속전압을 인가하여 전하를 띤 클러스터를 형성시켜 증착하였을 때 주상형 성장 모드는 사라졌으며, 직경 0.5$\mu$ m, aspect ratio 2의 접촉구에서 완벽한 바닥면의 도포성을 나타내었고, 측벽에의 증착성도 향상되어 막의 연결성이 개선되었다. 이로써 이온화 클러스터빔 증착법이 직진성을 향상시 켜 작은 접촉구의 메움을 향상시킬수 있는 물리적 증착 방법임을 확인하였다.

• 한국재료학회지
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• 제28권5호
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• pp.295-300
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• 2018

Effects of annealing temperature on the microstructure and mechanical properties through thickness of a Cu-3.0Ni-0.7Si alloy processed by differential speed rolling are investigated in detail. The copper alloy with a thickness of 3 mm is rolled to a 50 % reduction at ambient temperature without lubricant and subsequently annealed for 0.5 h at $200-900^{\circ}C$. The microstructure of the copper alloy after annealing is different in the thickness direction depending on the amount of the shear and compressive strain introduced by the rolling; the recrystallization occurs first in the upper roll side and center regions which are largely shear-deformed. The complete recrystallization occurs at an annealing temperature of $800^{\circ}C$. The grain size after the complete recrystallization is finer than that of the conventional rolling. The hardness distribution of the specimens annealed at $500-700^{\circ}C$ is not uniform in the thickness direction due to partial recrystallization. This ununiformity of hardness corresponds well to the amount of shear strain in the thickness direction. The average hardness and ultimate tensile strength has the maximum values of 250 Hv and 450 Mpa, respectively, in the specimen annealed at $400^{\circ}C$. It is considered that the complex mode of strain introduced by rolling directly affects the microstructure and the mechanical properties of the annealed specimens.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2003년도 추계학술대회 논문집
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• pp.213-219
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• 2003

As the 2-D hydraulic experimental results for the submerged rubble-mound structure, we have concerned with their stability/function characteristics of structures by the effects of wave force, scour/deposition at the toe and wave transmission ratio at the lee-side sea. And as to investigate the variation characteristics of wave transmission ratio which depended to a geometrical structure of the submerged breakwater profiles, the critical conditions for the depth of submergence and crest width obviously presented. In summary, there results lead us to the conclusions that the wave control capabilities of submerged breakwaters by the variation of the submergence depth is high about 4 time degrees at the efficiency than the that of crest width. The destruction of covering block at the crest generated at the region which located between maximum damage curve, it maximum damage/failure station from the toe of the structure were 0.2L. As the wave transmission coefficient and the slope of the structure increase, the damage/failure ratio and the maximum scour depth at the toe was extended, respectively. When maximum scour depth happened. The destruction of covering block which located at the toe generated at the front slope destruction. Finally, it was found from the results that the optimization of structure may be obtained by the efficiently decision of the submergence depth and crest width in the permissible range of wave transmission ratio.

• 셀메드
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• 제2권3호
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• pp.26.1-26.5
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• 2012

Adverse side-effects and lack of scientific validation of some chemotherapeutic agents prevent the use of many traditional medicines claimed to have anti-cancer effects. Ethanolic extract of Conium maculatum has long been used in traditional and alternative systems of medicine including homeopathy for the treatment of glandular enlargements, cancerous tumours or hard lumps of testicles, prostate, ovaries, breasts and/ or uterus, particularly in the breast. However, if and how it acts still remains scientifically unknown. This study aims to test if Conium extract (CE), used as mother tincture of Conium in homeopathy, has demonstrable anti-cancer potentials without having much cytotoxicity in normal cells. Cytotoxicity of the drug was tested by conducting MTT assay on both normal (peripheral blood mononuclear cells) and HeLa cells. We also evaluated DNA fragmentation and DNA damage by DAPI and diphenylamine assay. The LDH activity assay was done to evaluate the percentages of apoptosis and necrosis. ROS accumulation also was evaluated to pin-point the actual events of apoptosis. Administration of drug clearly demonstrated its anti-cancer potentials as evidenced by the DNA damage analysis. The ROS activity also increased in case of the CE treated cells. LDH data revealed that the mode of cell death was mainly apoptotic and not necrotic. CE appears to induce apoptosis of cancer cells through ROS mediated pathway, and has negligible cytotoxicity against normal cells.