• 디지털융복합연구
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• 제14권6호
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• pp.431-438
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• 2016

본 연구는 만 21-24세의 체육학 전공 대학생을 대상으로 키네시오 테이핑과 아이싱 처치가 혈중젖산농도 및 심폐기능의 회복속도에 미치는 영향을 규명하여 운동을 보다 효과적으로 수행할 수 있도록 경기력을 제한하는 요인들을 극복하여, 운동수행능력을 향상 시킬 수 있는 기초자료로 제공하는데 그 목적이 있다. 젖산과 심박수의 회복 속도를 측정을 통하여 얻은 결론을 본 연구에서 키네시오 테이핑과 아이싱 처치가 최대부하 운동 후 직후 젖산의 회복속도와 운동 30분 후 심박수의 회복속도를 유의하게 증가시켰으나, 운동 직후 심박수, 운동 15분 후 젖산 및 심박수, 운동 30분 후 젖산에서 유의한 차이가 없었다. 키네시오 테이핑과 아이싱 처치는 혈액 공급에 변화를 주어 통증이 감소되고 근 피로도 감소에 긍정적인 효과로 사용되고 있지만 만족스런 효과를 나타내지 못하였다. 이후 연구에서는 보다 정확하고 기술적인 형태의 운동 프로그램을 적용하여 키네시오 테이핑과 아이싱 처치의 효과에 대해 지속적인 연구가 필요할 것이다.

• Journal of Microbiology
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• 제37권2호
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• pp.102-110
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• 1999

Genetic determinant for the secondary metabolism was studied in heterologous expression in Streptomyces lividans TK-24 using Streptomyces griseus ATCC 10137 as a donor strain. Chromosomal DNA of S. griseus was ligated into the high-copy number Streptomyces shuttle plasmid, pWHM3, and introduced into S. lividans TK-24. A plasmid clone with 4.3-kb BamHI DNA of S. griseus (pMJJ201) was isolated by detecting for stimulatory effect on actinorhodin production by visual inspection. The 4.3-kb BamHI DNA was cloned into pWHM3 under the control of the strong constitutive ermEp promoter in both directions (pMJJ202); ermEp promoter-mediated transcription for coding sequence reading right to left: pMJJ203; ermEp promoter-mediated transcription for coding sequence reading left to right) and reintroduced into S. lividans TK-24. The production of actinorhodin was markedly stimulated due to introduction of pMJJ202 on regeneration agar. The introduction of pMJJ202 also stimulated production of actinorhodin and undecylproidigiosin in submerged culture employing the actinorhodin production medium. Introduction of pMJJ203 resulted in a marked decrease of production of the two pigments. Nucleotide sequence analysis of the 4.3-kb region revealed three coding sequences: two coding sequences reading left to right, ORF1 and ORF2, one coding sequence reading right to left, ORF3. Therefore, it was suggested that the ORF3 product was responsible for the stimulation of antibiotic production. The C-terminal region of ORF3 product showed a local alignment with Myb-related transcriptional factors, which implicated that the ORF3 product might be a novel DNA-binding protein related to the regulation of secondary metabolism in Streptomyces.

• 한국축산식품학회지
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• 제35권6호
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• pp.867-873
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• 2015

1,4-Dihydroxy-2-naphthoic acid (DHNA), a precursor of menaquinone (vitamin K2), has an effect on growth stimulation of bifidobacteria and prevention of osteoporosis, making it a promising functional food material. Therefore, we tried to clone the menB gene encoding DHNA synthase from Leuconostoc mesenteroides CJNU 0147. Based on the genome sequence of Leu. mesenteroides ATCC 8293 (GenBank accession no., CP000414), a primer set (Leu_menBfull_F and Leu_menBfull_R) was designed for the PCR amplification of menB gene of CJNU 0147. A DNA fragment (1,190 bp), including the menB gene, was amplified, cloned into pGEM-T Easy vector, and sequenced. The deduced amino acid sequence of MenB (DHNA synthase) protein of CJNU 0147 had a 98% similarity to the corresponding protein of ATCC 8293. The menB gene was subcloned into pCW4, a lactic acid bacteria - E. coli shuttle vector, and transferred to CJNU 0147. The transcription of menB gene of CJNU 0147 (pCW4::menB) was increased, when compared with those of CJNU 0147 (pCW4) and CJNU 0147 (−). The DHNA was produced from it at a detectable level, indicating that the cloned menB gene of CJNU 0147 encoded a DHNA synthase which is responsible for the production of DHNA, resulting in an increase of bifidogenic growth stimulation activity.

• 대한물리의학회지
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• 제3권1호
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• pp.1-9
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• 2008

Purpose : The purpose of this study is to assess the effects of ice immersion to the ankle, foot on vertical jump, isokinetic exercise. Methods : Thirty volunteers from universal students who had not sustained an injury to the lower extremity within the past 6 months were randomly assigned to either an experimental or control group. Subjects in the experimental group performed vertical jump and isokinetic exercise before and after the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot. Subjects in the control group performed vertical jump and isokinetic exercise before and after the application of a 15-minute resting. Results : Shuttle run was not significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Vertical jump was significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Speed $60^{\circ}$ of peak torque was significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Speed $120^{\circ}$ of peak torque was significantly decreased after than before the application of a 15-minute ice immersion($50-55^{\circ}F$) to the ankle and foot(p<0.05). Conclusion : we think because the ice immersion decrease active, physical therapist should carefully consider the consequence of cold therapy to increase active.

• 한국미생물·생명공학회지
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• 제14권2호
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• pp.161-168
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• 1986

E. coli-S. cerevisiae shuttle vector인 plasmid YRp7을 이용하여 B. amyloliquefaciens의 $\alpha$-amylase gene을 E. coli 내에 cloning하였다. 이때 제한 효소 Sau 3 AI에 의해 얻어진 $\alpha$-amylase gene의 크기는 약 1.95kb정도였으며 E. coli내에서 비교적 안정하게 유지되고 발현되었다. 재조합 plasmid p-EA24를 함유한 E. coli는 B. amyloliquefaciens의 약 65% 정도의 $\alpha$-amylase를 생성하였으며, 최적온도, pH, CaCl$_2$의 영향등 $\alpha$-amylase의 효소학적인 성질을 비교 조사해 본 결과 B. amyloliquefaciens의 $\alpha$-amylase와 동일하였다. 또한 E. coli에서 생성된 $\alpha$-amylase로 70% 정도가 periplasmic space에 존재하였으며 나머지는 세포 내부에 존재함을 알았다.

• 생약학회지
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• 제2권1호
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• pp.23-28
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• 1971

This study was planned to see whether the Panax Ginseng root has any influence upon the emotionality in rats exposed to a novel open-field and induced by a grid shock. Sixty four male albino rats whose body weight ranged from 130 to 180 gm were used. They were divided into four groups, i.e., placebo control group, 5mg/kg Ginseng ext. group, 10mg/kg Ginseng ext. group, and 20mg/kg Ginseng ext. group. Sixteen rats were subjected in each group. Open-field activity was measured in a $2.20m\;{\times}\;2.20m$ open-field that was divided into 15cm squares. Four novel objects and food pellets were put with an equal distance in the open-field. Locomotion was recorded by entering the number of squares traversed for 4 min. and behaviors which were shown at the 10-sec. period were checked by a modified Jarrad and Bunnel's observation procedure. Sensitivity to the grid shock was measured in a compartment of shuttle box. 10, 20, 30, 40, 50, 60 and 70 volt ac. 2 sec., electric shock were delivered in each subject by the grid to animal's feet. Flinch, vocalization and jumping responses were rated in each shock level. The results obtained were as follows: 1.) 5mg/kg and 10mg/kg Ginseng ext. groups were tended to increase the ambulation and exploration compared with placebo control. 2.) All Ginseng extract groups were defecated significantly less often than placebo control in the open-field and shock situation. 3.) Thresholds to the electric shock were tended to elevate in all Ginseng groups. 4.) These results suggest that Ginseng extract has a stimulant action in open-field and a tranqulizing action in feared situation.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.337-346
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• 2016

Three combinations of molecular chaperones from Escherichia coli (i.e., DnaK-DnaJ-GrpE-GroEL-GroES, GroEL-GroES, and DnaK-DnaJ-GrpE) were overproduced in E. coli BL21, and their in vitro stabilizing effects on a nitrile hydratase (NHase) were assessed. The optimal gene combination, E. coli groEL-groES (ecgroEL-ES), was introduced into Rhodococcus ruber TH3. A novel engineered strain, R. ruber TH3G was constructed with the native NHase gene on its chromosome and the heterologous ecgroEL-ES genes in a shuttle plasmid. In R. ruber TH3G, NHase activity was enhanced 37.3% compared with the control, TH3. The in vivo stabilizing effect of ecGroEL-ES on the NHase was assessed using both acrylamide immersion and heat shock experiments. The inactivation behavior of the in vivo NHase after immersion in a solution of dynamically increased concentrations of acrylamide was particularly evident. When the acrylamide concentration was increased to 500 g/l (50%), the remaining NHase activity in TH3G was 38%, but in TH3, activity was reduced to 10%. Reactivation of the in vivo NHases after varying degrees of inactivation was further assessed. The activity of the reactivated NHase was more than 2-fold greater in TH3G than in TH3. The hydration synthesis of acrylamide catalyzed by the in vivo NHase was performed with continuous acrylonitrile feeding. The final concentration of acrylamide was 640 g/l when catalyzed by TH3G, compared with 490 g/l acrylamide by TH3. This study is the first to show that the chaperones ecGroEL-ES work well in Rhodococcus and simultaneously possess protein-folding assistance functions and the ability to stabilize and reactivate the native NHases.

• International Journal of Ocean Engineering and Technology Speciallssue:Selected Papers
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• 제5권1호
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• pp.22-28
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• 2002

A New concept for the LNG-FPSO ship, with moonpool and bilge step in bottom, is proposed. This concept is investigated with regard to motion reduction and sloshing phenomena of the cargo and operation tanks. The principal dimensions of the ship are $L\timesb B\times D\times t(design)=270.0\times51.0\times32.32\times13.7(m)$, with a total cargo capacity of 161KT; a 98% loading condition is considered for this study. The moonpools and rectangular step at the bilge have been designed for the purpose of decreasing the motion within the tank. For the motion analysis, linearized three-dimensional diffraction theory, with the simplified boundary condition was used. The six-degree of freedom coupled motion responses were calculated for the LNG-FPSO ship. Viscous effects on the roll motion responses of a vessel were taken into account in this calculation program, using an empirical formula suggested by Himeno(1981). The case study for the moonpool size has been conducted using theoretical estimation and the experimental method. For the optimization of the moonpool size and effect of the bilge step, 9 cases of its size, both with and without bilge step, were involved in the study. no motion responses, especially roll motion, for the designed LNG-FPSO ships are much lower than those of other drill ships and shuttle tankers. The limit criterions are satisfied. To check the cargo tank and operation tank sizes, we performed a sloshing analysis in the irregular waves which focuses on the pressure distribution on the tank wall and the time history of pressure and free surface for No.2 and 5 tanks of LNG-FPSO with chamfers. Finally, optimum tank sire was estimated.

• Journal of Electrochemical Science and Technology
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• 제13권2호
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• pp.199-207
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• 2022

Lithium-sulfur batteries (LSBs) have emerged as a promising alternative to lithium-ion batteries (LIBs) owing to their high energy density and economic viability. In addition, all-solid-state LSBs, which use solid-state electrolytes, have been proposed to overcome the polysulfide shuttle effect while improving safety. However, the high interfacial resistance and poor ionic conductivity exhibited by the electrode and solid-state electrolytes, respectively, are significant challenges in the development of these LSBs. Herein, we apply a poly (ethylene oxide) (PEO)-based composite solid-state electrolyte with oxide Li₇La₃Zr₂O₁₂ (LLZO) solid-state electrolyte in an all-solid-state LSB to overcome these challenges. We use an electrochemical method to evaluate the degradation of the all-solid-state LSB in accordance with the carbon content and loading weight within the cathode. The all-solid-state LSB, with sulfur-carbon content in a ratio of 3:3, exhibited a high initial discharge capacity (1386 mAh g^-1), poor C-rate performance, and capacity retention of less than 50%. The all-solid-state LSB with a high loading weight exhibited a poor overall electrochemical performance. The factors influencing the electrochemical performance degradation were revealed through systematic analysis.

• 한국건설관리학회논문집
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• 제25권5호
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• pp.55-62
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• 2024

본 연구는 국내 건설현장의 우수한 기술인력 양성 및 확보를 위해, 현재 시행중인 교육·훈련에 대한 효과 및 개선 필요성을 분석하고, 이에 대한 향후 개선방안을 제시함을 목적으로 연구를 수행하였다. 연구를 통해 얻은 결론은 다음과 같다. 건설현장 기술인력들의 현행 교육에 대한 만족도는 전반적으로 높고 직무능력향상에도 효과가 있는 것으로 조사되었으나, 교육내용, 교육방법, 교육환경은 다소 개선이 필요한 것으로 나타났다. 교육내용 개선을 위해서는 기술발전에 부응하는 실무 위주교육 커리큘럼의 도입이 시급히 필요하다. 온라인 교육방법의 개선을 위해서는 우수한 교육 컨텐츠의 개발이 필요하다. 교육환경개선을 위해서는 맞춤형 실습공간의 확충, 권역별 교육시설 설립, 셔틀버스 운행 등이 필요하다.