• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.1479-1482
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• 2004

LMTT (Linear Motor-based Transfer Technology) is horizontal transfer system in the maritime container terminal for the port automation. The system is driven by PMLSM (Permanent Magnetic Linear Synchronous Motor) that is consists of stator modules on the rail and shuttle car. This paper investigates the effect of the drop height of container on impact reaction force of the Impact Absorber (IA) in shuttle car for LMTT. The results of this investigation are obtained from detailed finite element analysis for various parameters, such as the spring coefficient, the drop height of container.

• 미생물학회지
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• 제29권3호
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• pp.149-154
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• 1991

Escherichia coli/Corynebacterium glutamicum shuttle vectors, pECCG1 and pECCG2 were constructed by joining a 3.00 kb cryptic plasmid pCB 1 from C. glutamicum and a 3.94 kb plasmid pACYC 177 from E. coli. By trimming unessential parts and introducing mulitiple cloning site into the plasmid pECCG 1, a plasmid pECCG122(5.1kb) was constructed. All the shuttle vectors were stably maintained in C. glutamicum up to about 40 generations irrespective of kanamycin addition in the medium. Threonine operon (homoserine dehydrogenase/homoserine kinase) and dapA gene (dihydrodipicolinate synthetase) of C. glutamicum were cloned into the plasmid pECCG122, and the resultant plasmids were designated pTN31 and pDHDP19, respectively. They were used to study the effect of cloned foreign gene on the stability of the plasmid pECCG122. Plasmids pTN31 and pDHDP19 were segregated rapidly from C. glutamicum when cultured in the medium without kanamycin. In medium with $50\mu${\g/ml} of kanamycin, their segregation rates were much slower than those in medium without kanamycin, but the danamycin addition didn't guarantee the complete maintenance of the plasmids in C. glutamicum.

• Journal of Ship and Ocean Technology
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• 제9권1호
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• pp.11-26
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• 2005

In this study, the program to investigate the multiple body interaction effects between a floating platform and a shuttle tanker considering the coupled effect of hull (FPSO) with mooring lines and risers was developed. The coupled analysis program, which is called WINPOST-MULT using the hydrodynamic analysis results by WAMIT, was made. For the verification of WINPOST-MULT by means of numerical experiments, two multiple-body models of an FPSO-FPSO and an FPSO-shuttle tanker system are adopted. With the FPSO-FPSO model and a two-mass-spring system to idealize two identical bodies for the 100-year storm wave condition in GOM, the numerical simulations were performed to investigate the interaction effects between two identical bodies. For the more reality, the coupled analysis for the FPSO-shuttle tanker model in the tandem arrangement was carried out in the consideration of the environmental condition of the West Africa Sea as a rather mild condition. Through the case studies with interaction effect and without interaction effect by the iteration method and the combined method, it is verified that the program is a very useful tool for the analysis of the interaction problem of multiple-body system and the coupled problem of the hull/mooring/riser.

• Journal of Biosystems Engineering
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• 제28권3호
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• pp.187-198
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• 2003

This study was conducted to investigate the effect of design parameters of modulating valve and hydraulic clutch on the shift quality of a power shuttle transmission using a computer simulation. Computer simulation models of a hydraulic control system and a power shuttle drive train were developed and verified by an experimental power train in a laboratory. The software EASY5 was used for the modeling and simulation of the power shuttle transmission. Results of the study were summarized as follows: For a good shift quality. it is required to reduce the transient torque transmitted to the output shaft of the transmission as much as possible. This may be achieved by reducing the modulating time and clutch pressure. It was found that the design parameters most significantly affecting the modulating time and clutch pressure were the spring constant and displacement of a load piston of the modulating valve, and the spring constant and damping of the clutch piston. The modulating time decreased as the spring constant increased and increased as the displacement of the load piston decreased. The transient torque decreased as the modulating time increased. However their relationships were not always linear. As the damping decreased, both the modulating pressure and time decreased, which also resulted in a decrease in the transient torque. The spring constant of the clutch piston affected the modulating time and the peak transient torque. As the spring constant of the clutch increased, the peak transient torque decreased.

• 한국게임학회 논문지
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• 제14권2호
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• pp.67-76
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• 2014

최근 다양한 모션 센서를 이용해서 실제 사용자의 동작을 인식하는 체감형 스포츠 게임에 대한 관심이 높다. 본 논문에서는 체감형 게임 플레이를 지원하는 배드민턴 게임의 구현에 필요한 핵심 요소 기술인 스윙 모션의 인식과 셔틀콕의 궤적 계산 방법을 제안한다. 사용자가 스마트폰을 손에 쥐고 배드민턴 스윙을 하면, 스마트폰에 내장된 가속도 센서가 발생시키는 모션신호를 다우비시 필터를 이용해서 특징벡터로 변환하고, 이를 k-NN 기반의 인식을 통해서 스윙 타입을 알아낸다. 본 논문에서 제안한 스윙 모션 인식 방법을 이용하면, 상용 모션 콘트롤러를 구입하지 않아도 체감형 배드민턴 게임을 즐길 수 있는 장점이 있다. 배드민턴 셔틀콕은 그 모양의 특징으로 인해 독특한 비행 궤적을 가지고 있기에, 단순한 힘과 속도에 관한 물리 법칙으로는 그 궤적을 표현하기 쉽지 않다. 본 논문에서 우리는 바람의 영향을 고려한 배드민턴 셔틀콕의 비행 궤적 계산 방법을 제안한다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2004년도 추계학술대회
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• pp.323-328
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• 2004

세계 컨테이너 무역 규모가 연간 $7\%$씩 증가함에 따라 항만 환경이 급격히 변화하고 있다. 이러한 항만의 변화에 성공적으로 대처하기 위해 차세대 항만하역시스템인 LMTT(Linear Motor-based Transfer Technology)의 연구가 진행되고 있다. LMTT용 Shuttle car의 frame부는 컨테이너를 지탱하는 outer beam, frame의 뼈대가 되는 inner beam, 두 beam을 연결시켜 보강하는 cross beam으로 구성되어 있으며, 본 연구에서는 이러한 frame은 설계하기 위하여 cross beam의 개수, 하중 재하 위치 및 inner beam의 위치에 따른 outer beam과의 거리 비 등이 frame의 강도 및 강성에 미치는 영향을 유한요소해석을 통하여 고찰하였다.

• 대한조선학회논문집
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• 제43권5호
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• pp.560-567
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• 2006

FPSO and shuttle tanker are connected to each other by a mooring hawser and a loading hose through which cargo oil is off-loaded. Even in mild sea-state. environmental loads can cause unstable large drift motions between two vessels in tandem off-loading operations, which may result in collision incidents. Accordingly. the analysis on the relative motion between two vessels due to the environmental loads should be investigated in initial design stage. In this study, the low speed maneuvering equation is employed to simulate nonlinear motions of FPSO and shuttle tanker. Low frequency wave drift forces including hydrodynamic interactions between two vessels are evaluated by near field approaches. Current loads are determined by mathematical model of MMG and wind loads are calculated by employing the wind spectrum according to the guidelines of API-RP2A. Mooring forces produced by turret mooring lines and a flexible hawser are modeled quasi-statically by catenary equations. The effect of environmental loads that affect nonlinear motion is investigated through variation in their magnitudes and the nonlinear motions between FPSO and shuttle tanker are simulated under wave, current and wind in time domain.

• 한국항해항만학회지
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• 제29권1호
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• pp.77-82
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• 2005

컨테이너 무역 규모가 매년 증가함에 따라 항만 환경이 급격히 변화하고 있다. 이러한 항만의 변화에 성공적으로 대처하기 위해 차세대 항만하역시스템인 LMTT(Linear Motor-based Transfer Technology)의 연구가 진행되고 있다. LMTT용 셔틀 카의 프레임부는 내부 빔, 내부 빔, 크로스 빔으로 구성되어 있으며, 본 연구에서는 프레임을 설계하기 위하여 크로스 빔의 개수, 하중 재하 위치 및 내부 빔의 위치에 따른 외부 빔과의 거리비 등이 프레임의 강도 및 강성에 미치는 영향을 유한요소 해석을 통하여 하중이 외부 빔과 내부 빔에 동시에 작용하고 크로스 빔이 5개일 때가 최적의 조건이라는 결론을 얻을 수 있었다.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 1996년도 제19회정기학술대회(The 19th Symposium of the Korean Society of Environmental Toxicology)
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• pp.64-64
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• 1996

Transgenic animal and cell line models which are recently developed in toxicology field combined with molecular biological technique, are powerful tools for studying of mutation in vivo and in vitro, respectively. The Big Blue mutagenesis assay system is one of the most widely used transgenic systems. Especially, for the study of direct acting mutagens, Big Blue cell line is very useful and powerful to evaluate mutagenicity because the mutation frequency and mutationspectrlun showed no distinct differences between cell line and animal. The Big Blue cell lines carry stably integrated copies of lambda shuttle vector containing lac I gene as a mutational target. These lambda shuttle vectors are useful for various mutagenesis related studies in eukaryotic system due to their ability to be exposed mutagen and then transfer a suitable target DNA sequence to it convenient organism for analysis. We tried to assess the mutagenic effect of 4-NQO with Big Blue cell line. After the treatment of 4-NQO, genomic DNA was isolated and lambda shuttle vector was packaged by in Vitro packaging and then these were plated on bacterial host in the presence of X-gal to screen mutation in the lac I. We determined MF as a ratio of blue plaques versus colorless plaques and now undergoing the mutation spectrum of 4-NQO in lac J gene sequence.

• 한국미생물·생명공학회지
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• 제26권5호
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• pp.400-405
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• 1998

본 연구는 B. lactofermentum에서 ddh유전자의 증폭이 lysine 생성량에 미치는 영향을 조사하기 위하여, 먼저 E. coli-B. lactofermentum shuttle vector pEB1 및 pEB2를 제조하였으며, 제조된 shuttle vector에 ddh 유전자를 ligation하여 재조합 plasmid pRK1 및 pRK2를 구축하였다. B. lactofermentum에서 ddh 유전자를 증폭시키기 위하여 재조합 plasmld를 B. lactofermentum으로 도입하여 DDH 활성을 측정한 결과 대조균보다 7배 정도 증가하였다. 또한 lysine 생성량의 비교 분석에서는 재조합 plasmid를 함유한 균주의 경우 48시간 이후부터 control 균주보다 lysine 생성량이 증가하기 시작하여 72시간 때에는 최대치를 나타내었으며 그 이후는 오히려 감소하였다. 최대치를 나타낸 72시간 때의 lysine 생성량은 대조균주가 4.30∼4.38 g/l를 나타내었으며 pRK1 및 pRK2를 함유한 균주는 각각 5.34 g/l 및 5.07 g/l이었다. 이상의 결과로 미루어 볼 때 ddh유전자의 증폭에 의한 B. lactofermentum의 lysine 생성량은 대조균주에 비하여 18∼20% 정도 증가하였다.