• BMB Reports
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• v.34 no.2
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• pp.114-117
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• 2001

Cervi Parvum Cornu is widely used as a hemopoietic, tonifying, growth-promoting, cardiotonic, and immuno-modulating agent in Korea. In order to develop the quality control method of Cervi Parvum Cornu by the identification of the biological source or origin, the molecular approach was applied using PCR (polymerase chain reaction) and PCR-RFLF (PCR-restriction fragment length polymorphism) analysis. In the PCR analysis of the mitochondrial 12S rRNA gene and cytochrome b gene regions, no distinctive DNA bands from Cervidae (deer) antlers and Rangifer (reindeer) antlers were observed. However, when the amplified products in the mitochondrial cytochrome b gene region were subjected to restriction digestion with TaqI, Cervidae antlers showed an undigested state of 380 by band, differently from two bands of 230 by and 1S0 by from Rangifer antlers. Based on this finding, the base sequences of amplified PCR products in the range of mitochondria) cytochrome b gene from Cervidae antlers and Rangifer antlers were determined and subjected to restriction analysis by various endonucleases. The results showed that antlers from Rangifer species could be simply discriminated with other antlers from 8 Cervidae species (Chinese deer, Russian deer, Hong Kong deer, New Zealand deer, Kazakhstan deer, elk, red deer and Sika deer) by PCR-RFLP analysis using AtuI, HaeIII, HpaII or Sau3AI(MboI) as well as TaqI in the range of the mitochondrial cytochrome b gene.

• The KIPS Transactions:PartD
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• v.10D no.4
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• pp.671-678
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• 2003

A digital video is usually very long temporally. requiring large storage capacity. Therefore, users want to watch pre-summarized video before they watch a large long video. Especially in the field of sports video, they want to watch a highlight video. Consequently, highlight video is used that the viewers decide whether it is valuable for them to watch the video or not. This paper proposes how to create soccer video highlight using the structural features of the caption such as temporal and spatial features. Caption frame intervals and caption key frames are extracted by using those structural features. And then, highlight video is created by using scene relocation, logical indexing and highlight creation rule. Finally. retrieval and browsing of highlight and video segment is performed by selection of item on browser.

• Journal of radiological science and technology
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• v.15 no.1
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• pp.99-105
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• 1992

Visual evaluation of rib shadow and lung marking during high voltage chest radiography. The Purpose of this study is to improvement of visual discrimination of pulmonary structures on the conventional chest radiogram. The author prepared an artificial lung using an acryl plate, 8 cm in thickness, which is nearly equivalent to human lung, and 0.6 cm thickness of an aluminum plate for an artificial rib, and 0.5 cm of an acryl plate as a pulmonary vessel as well. And they were used as objects for experimental radiograms. This study performed with gradual increasing densities of film bases in the sequences of densities of 0.6, 0.9, 1.1 and 1.3. We made two combinations of images after multiple and regular cuts, with width of 1 cm, of 4 radiograms at the above mentioned densities of film bases. One image consisted of alternative combination of radiograms taken at densities of 0.6 and 1.3, and the other did at 0.9 and 1.1. The latter image provided better visual perception of pulmonary structures than the former. Experimental radiograms were also taken with 60 kV and 120 kV respectively. After careful evaluation and comparison to images taken on varieties of different densities with combinations and kV, the author had a conclusion that it is advisable to use a high kV X-ray which makes rib shadow subtle, for better visual delineation of pulmonary structures behind ribcage, eventhough contrast of pulmonary structures are decreased at high kV radiogram.

• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.37-45
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• 2006

Treatment with exopolymers of Aureobasidium pullulans SM-2001 containing $\beta-1,3/1,6-glucan$ inhibited osteoclastogenesis of bone marrow stem cells in a co-culture system with calvariae osteoblastic cells. In addition, the treatment increased mineral deposition in osteoblastic cells. These two observations prompted us to evaluate whether the exopolymers could be used as an anti-osteoporotic agent, and efficacy of the exopolymers to prevent bone loss was compared with alendronate, a bisphosphonate, in ovariectomized mice prone to osteoporosis. Administration of the exopolymers to the ovariectomized mice resulted in improved effects on femur weight and histomorphometric changes of femur such as trabecular bone volume (TBV), trabecular bone thickness (TBT), and cortical bone thickness (CBT). In conclusion, the exopolymers treatment inhibited bone loss from osteoporosis induced by ovariectomy, and the effect was comparable to alendronate administration.

• Clinics in Shoulder and Elbow
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• v.13 no.1
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• pp.106-110
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• 2010

Purpose: We report a case of pyogenic arthritis of the shoulder secondary to infective endocarditis. Materials and Methods: A 70 year-old male who had suffered from pyogenic arthritis of the left shoulder secondary to infective endocarditis was treated with artificial valvuloplasty, arthroscopic synovectomy and drainage. Results: Infection was cured and the patient achieved a good functional outcome. Conclusion: Pyogenic arthritis of the shoulder is rarely associated with infective endocarditis. However, if the symptoms are misdiagnosed as musculoskeletal symptoms associated with infective endocarditis, serious complications may arise. As such, musculoskeletal symptoms associated with infective endocarditis should be paid careful attention.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.612-619
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• 2000

To investigate the biochemical properties of V. mimicus metalloprotease, whose gene was isolated previously from Vibrio mimicus ATCC33653, overexpression and purification were attempted. The 1.9 kb of open reading frame was amplified by PCR from pVMC193 plasmid which ligated the VMC gene with pUC19 and introduced into Escherichia coli BL21 (DE3) using the overexpression vector, pET22b (+). The overexpressed metalloprotease (VMC) was purified with Ni-NTA column chromatography and characterized with various protease inhibitors, pHs, temperatures, and substrates. The purified VMC showed the proteolytic activity against gelatin, soluble and insoluble collagens, and synthetic peptides. Unlike the observations made with all metalloproteases originated from other Vibrio sp., the VMC did not hydrolyze the casein. The proteolytic activity was critically decreased when the VMC was treated with metal chelating reagents, such as EDTA, 2,2-bipyridine, and 1, 10-phenanthroline. In particular, the 71 kDa VMC exhibited the hemagglutinating activity against human erythrocyte. As the purified VMC was treated with $CuCl_2$ and $NiCl_2$ for the chemical modification of metal binding, the proteolytic activity and hemagglutinating activity were profoundly influenced. The multialignment analysis made on the reported Vibrio metalloproteases showed the difference of amino acid sequence similarity between the two distinctive classes of Vibrio metalloproteases.

• Journal of Microbiology and Biotechnology
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• v.28 no.9
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• pp.1433-1442
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• 2018

To identify and quantify the effects of a combination of dietary $1{\times}10^8CFU/g$ Lactococcus lactis subsp. lactis I2 ($LI_2$) and 0.1% ${\beta}$-glucooligosaccharides (BGO) on the growth and immunity of olive flounder (Paralichthys olivaceus), a feeding experiment was conducted. Flounder ($14{\pm}0.5g$) were divided into two groups and fed control and synbiotic feeds for 8 weeks. Investigations were carried out on growth and feed utilization, innate immunity, serum biochemical parameters, intestinal lactic acid bacterial (LAB) viability, microvillus length, and changes in the expression levels of genes encoding pro-inflammatory cytokines (tumor necrosis factor $[TNF]-{\alpha}$, interleukin $[IL]-1{\beta}$, and IL-6). Results demonstrated the synbiotic diet had significantly better (p < 0.05) responses in terms of weight gain and specific growth rate, three innate immune parameters (respiratory burst, serum lysozyme, and superoxide dismutase), intestinal LAB viability, and the relative $TNF-{\alpha}$ expression level (p < 0.05). Moreover, after challenge with Streptococcus iniae ($1{\times}10^8CFU/ml$), the synbiotically fed group exhibited significantly higher (p < 0.05) protection against streptococcosis, validating the observed changes in immune parameters and induction of the cytokine-encoding gene. Therefore, according to the results of the present study, synbiotic feed ($LI_2+BGO$) increased growth, modulated innate immune parameters and protected olive flounder against streptococcosis.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.112-117
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• 2011

This research was conducted to determine the effect of the pre-treatment of mulberry charcoal on the salt tolerance response in Kentucky bluegrass 'Perfection'. As this results, 400 mM NaCl treatment reduced turf growth such a fresh and dry weight, and induced physiological damages like decreased chlorophyll content and increased electrolyte leakage. The pre-treatment of mulberry charcoal, however, mitigated those growth and physiological responses associated with NaCl stress, regardless of the concentrations of charcoal solution. Furthermore, it was observed that higher K ion concentration by mulberry charcoal pre-treatment reduced Na ion in shoot. This research suggests to be a possibility of planting turf grasses in reclaimed land or higher salt accumulated area using a mulberry charcoal treatment.

• The Journal of the Korea Contents Association
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• v.16 no.5
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• pp.686-697
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• 2016

The present research investigates the reciprocal effects of a previous brand extension and follower brand extension's market performance on a parent brand of a follower brand's extension product using 2(previous brand extension's market performance: success vs. failure)${\times}$2(follower brand extension's market performance: success vs. failure) between-subjects factorial design. The verification of the hypothesis is composed through 2-way ANOVA. As a result, the consumers' evaluation of the parent brand of a follower brand's extension product when the follower brand's extension succeed was more positive when the previous brand's extension has failed. However, when the follower brand's extension has failed, the consumers' evaluation of the parent brand of a follower brand's extension product was more negative when the previous brand's extension has succeeded.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2000.10a
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• pp.324-327
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• 2000

There are developing the World Wide Web systems for their information sharing among certain closed user group. These systems are required five common functions as follows. First, they have to support the web service for Internet users to access easily. Second, Hey have to support the authorization service to confirm the allowed users. Third, they have to support the database service to manage the sharing information among users. Fourth, they have to support the bulletin board service for closed users to discuss. Fifth, they have to support the e-mail service to send e-mail to the certain user subset and mail archive service to save and manage the previous mail information. In this paper, we described the detail function and processing procedure of our implemente system under LINUX operating system. In addition, we described problems occurring in implementation and their solutions.