• Title/Summary/Keyword: shepherd

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PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea

  • Kim, Yun-Ah;Kim, Hye-Youn;Cho, Shin-Hyeong;Cheun, Hyeong-Il;Yu, Jae-Ran;Lee, Sang-Eun
    • Parasites, Hosts and Diseases
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    • v.48 no.1
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    • pp.9-13
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    • 2010
  • Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performed to confirm the P. hominis infection and its molecular characterization from the feces of puppies with diarrhea. Fecal samples were obtained from 14 German shepherd puppies with diarrhea over 1 week (7 females and 7 males, 2-9 months of age) residing on a dog farm in August 2007. Species-specific PCR assay identified P. hominis 18S rRNA genes in 3 of the 14 puppies (1 female and 2 males; 1 aged 2 months and 2 aged 9 months). This phylogenetic analysis established that P. hominis belonged to the 1st clade, which is comprised of Bos taurus and Felines.

Studies on the types of hemoglobin and albumin in German shepherds (군용견(軍用犬) German shepherd의 hemoglobin 및 albumin형(型)에 관(關)한 연구(硏究))

  • Park, Kwon-mu;Kang, Ju-won;Han, Bang-keun
    • Korean Journal of Veterinary Research
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    • v.34 no.3
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    • pp.487-492
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    • 1994
  • The study was conducted to investigate the phenotypes of blood proteins, hemoglobin and albumin, of 104 German shepherds in Dag-gu, Yae-chum Kim-hae and Kwang-ju area by the starch gel electrophoresis. The results obtained were summarized as follows: 1. The hemoglobin phenotypes were observed three(HbBB, HbBb and Hbbb). Fast migrating bands were same and slow migrating bands were divided by heavy(HbBB), light(HbBb) and non-stained(Hbbb). The frequencies of appearance in HbBB, HbBb and Hbbb were 17.31%, 51.92% and 30.77%, respectively. 2. The hemoglobin phenotypes were controlled by two allelic genes, $Hb^b$ and $Hb^B$. The gene frequencies were calculated at 0.567 in $Hb^B$ and 0.433 in $Hb^A$. 3. The albumin phenotypes were observed three(AlbFF, AlbSS and and AlbFS), which were divided by fast migrating band(AlbFF), slow migrating band(AlbSS) and mixed migrating band(AlbFS). The frequencies of appearance in AlbFF, AlbFS and AlbSS were 5.77%, 28.85% and 65.38%, respectively. 4. The albumin phenotypes were controlled by two alleic genes, $Alb^F$ and $Alb^S$. The gene frequencies were calculated at 0.202 in $Alb^F$ and 0.798 in $Alb^S$.

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Tear formation, the patency and the angle of bend of nasolacrimal duct in poodle dogs with tear staining syndrome (Tesr staining syndrome이 있는 poodle에서 눈물생산, 비루관 개통성 및 굴곡도)

  • Seo, Kang-moon;Nam, Tchi-chou
    • Korean Journal of Veterinary Research
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    • v.35 no.2
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    • pp.383-390
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    • 1995
  • This study was performed to investigate the characteristics of tear staining syndrome in poodle dogs. Schirmer tear test, fluorescein dye test and measurement of the angle of bend between vertical and horizontal bony nasolacrimal duct were conducted in both poodles and German shepherd dogs. There were no significant differences between normal and tear-stained poodles in tear formation determined by Schimer tear test. However, there was significantly higher tear production in German shepherds than that in normal poodles(p<0.05). In the fluorescein dye test for the measurement of tear excretion, the dye was observed within $14.5{\pm}6.5$ minutes after dropping of the dye in normal poodles, but was not observed even over 30 minutes in tear-stained poodles. German shepherds had rather rapid passage time($0.4{\pm}0.3$ minutes) than poodles in the dye excretion. In the measurement of the angle of bend between vertical and horizontal bony nasolacrimal duct through dacryocystorhinography, there were no significant differences between normal tear-stained poodles with showing $85.0{\pm}6.8^{\circ}$ and $89.8{\pm}6.5^{\circ}$, respectively. However, obtuse angle of bend($106.8{\pm}4.7^{\circ}$) was shown in German shepherds. These results have ascertained that tear staining syndrome of poodle dogs was not related to tear production but to the rate of tear excretion.

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Comparison of Angle Measurements on Hallux Valgus with Two Different Methods Using Digital Images (디지털 영상을 이용한 무지 외반증 변형각 측정에서 서로 다른 두 계측 방법의 비교)

  • Sung, Il-Hoon;Kim, Ki Chun;Sung, Chang-Ho;Seo, Woo-Young;Lee, Doo-Yeon;Cho, Young A
    • Journal of Korean Foot and Ankle Society
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    • v.17 no.1
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    • pp.40-44
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    • 2013
  • Purpose: To study inter- and intra-observer reliabilities of computerized measurements of the angular parameters of hallux valgus deformity, using two different kinds of software tools for angle measurement on the digital radiography. Materials and Methods: On 35 digital radiographies of standing foot anteroposterior view of hallux valgus, two observers (A, B) independently measured hallux valgus angle (HVA) and 1-2 intermetatarsal angle ($IMA_{1-2}$) twice, using two methods. In method I, an angle was determined from duplicated lines to longitudinal axes made for bisecting line on the target bones with software tool. In method II, an angle was calculated automatically and directly from bisecting lines (longitudinal axes) made on the target bones. We compared two methods using paired t-test to determine significance of differences. Inter- and intraobserver reliabilities were evaluated using the intraclass correlation coefficients (ICC). Results: There were no significant differences between measurements of method I and II for each observer (p>0.05) and intraobserver reliability were good. (ICC>0.9) Inter-observer reliability for method I and II was good of the HVA (ICCs, 0.912 and 0.905) and moderate of the $IMA_{1-2}$ (ICCs, 0.505 and 0.537). There were interobserver differences in HVA of method I and II. Conclusion: No significant difference was found statistically between measurements of method I and II. Both methods I and II would be acceptable to measure angular parameters of hallux valgus deformity.

Radiographic Diagnosis of Degenerative Joint Disease in Canine Hip Joint (개의 고관절 변성관절질환의 방사선학적 진단)

  • 엄기동;장동우;서민호;정주현;장진화;원성준;정우조;최호정;이기창
    • Journal of Veterinary Clinics
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    • v.18 no.3
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    • pp.243-248
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    • 2001
  • Radiographic findings of degenerative joint disease (DJD) in the coxofemoral joints were studied in 108 dogs referred to Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University. Radiographic findings were identified with a extended ventrodorsal projection. Clinical signs such as pain in the affected joint, exercise intolerance, lameness, crepitation on flexion and extension of the joint and decreased range of motion, were shown in 40% and radiographic changes of bilateral coxofemoral joints were identified in 69% of a group of dogs. There are no sex predilections. Thirty-seven dogs were diagnosed as canine hip dysplasia (CHD; 10 cases), avascular necrosis of the femoral head (Legg-Calves-Perthes disease [LCPD]; 12 cases), fracture of the acetabulum (1 case), luxation(10 cases) and subluxation (15 cases), however, luxation and subluxation were identified in 11 dogs affected with CHD and LCPD. Causes of DJD in others (71case) were not identified. Radio-graphic findings identified included osteophyte formation at the attachment of the joint capsule (70%), sclerosis of the subchondral bone of the cranial acetabular edge (47%), remodeling of the femoral head (34%), thickening of the femoral neck(31%), perichondral osteophyte formation (31%), joint laxity (30%) and so on. All of the dogs diagnosed as CHD were large breed such as Retriever, Germain Shepherd, and Weimaraner and their age was under 1 year. This condition occurred bilaterally in 70% of affected animals. Dogs diagnosed as LCPD were young (under 1 year), small breed dogs. The incidence of LCPD is greater in Yorkshire Terrier, Miniature Pinscher and Poodle. This condition occurred unilaterally in 67% of affected animals. Clinical signs of dogs with unknown cause were shown in 15% and this condition occurred bilaterally in 75% of affected animals. Radiographic findings varied with patient's age and breed, however, the most common finding was osteophyte formation at the attachment of the joint capsule.

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Occurrence of Geotrichum candidum in Two Cases of Canine Dermatitis (두 마리 개의 피부염에 있어서 Geotrichum candidum의 존재)

  • Mahendra Pal;Chang woo Lee;Naonori Matsusaka
    • Journal of Veterinary Clinics
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    • v.19 no.4
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    • pp.433-435
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    • 2002
  • Ceotrichum candidum was identified from 2 dogs among 52 dogs which had various dermatological problems. One was 5-month-old male Labrador and the other was 7-month-old female German Shepherd. The diagnosis was confirmed by direct microscopy of the specimen as well as cultured isolation of the fungus from the cutaneous lesions. The. organism showed septate hyphae and rectangular to cylindrical arthrospores and no blastospores. Urea was not utilized and no assimilation of maltose was observed. Clinical improvement was occurred by application of 2% gentian violet and 2% miconazole solutions. It is suggested that this organism should be included in the differential diagnosis of canine dermatitis.

The Systematic Study on Reduction of Food Waste Products(I) -Survey on Waste Rates of Frequently Consumed Korean Foods and Effect of Education on Kitchen Wastes of Institutional Foodservices - (음식물 쓰레기를 줄이기 위한 체계화 연구(I) -상용식품의 폐기율 조사와 급식소의 유형별 음식물 쓰레기량과 교육에 의한 개선효과-)

  • 전예숙;최미경
    • Journal of the East Asian Society of Dietary Life
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    • v.9 no.1
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    • pp.55-63
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    • 1999
  • This research was conducted to estimate waste rates of 69 food items after pre-preparation and amounts of food wastes disposed and it's reduction effect by education in some institutional foodservices for reduction of the food wastes. The result are as follows: 1. The waste rates were 55.40% for sweet corn, 5.73% for potatoes, 19.14% for sweet potatoes, and 33.47% for chestnuts. 2. The waste rates of fruits were 0.21-49.36%. Food items with over 40% waste rate were banana, watermelon, and pineapple. 3. The waste rates of vegetables were 1.14-52.90%. The number of foods with under 10% waste rate were 14 items(red pepper, green pepper, chard, and so on), 10-20% was 9 items(perilla leaf, chinese cabbage, ginger root, and so on), 20-30% was 5 items(root of chinese bellflower, garlic, radish-leaves, burdock, welsh onion-large type), 30-40% was 3 items(shepherd's purse, head lettuce, kale) and over 40% was 3 items(water dropwort, crown daisy, mallow). 4. The waste rates were 24.30% in chicken, 9.53-13.79% in eggs, and 9.30-55.32% in fishes. The waste rates of vegetables and fishes were higher than those of other food groups. 5. There were significantly different in amount of food wastes disposed (g/person/day) to institutional foodservices (hospital>industrial institution> Korean restaurant>elementary school). The amount of food wastes disposed, especially amount in pre-preparation phase, after education for reduction of food wastes was significantly reduced. Since these study results show significant deviations in food waste rates and education effect, there should be more studies for standard waste rates of each food and systematic education method for reduction of food wastes.

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The Order of Appetites in Early Modern England: Shakespeare's Signs of Food and Social Mobility (초기 근대 영국의 미각의 질서 -셰익스피어 희곡의 음식 기호와 사회적 유동성)

  • Roh, Seung-Hee
    • Journal of English Language & Literature
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    • v.57 no.1
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    • pp.171-190
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    • 2011
  • Shakespeare's plays deploy an interesting array of food signs in a way to illuminate the historical process of what Stephen Mennell has described as "the civilizing of appetite"-a process in which the changes of food choices and eating habits took place in response to the changes in people's way of life and personality structure over the long-term modern period since the middle ages. Shakespeare's plays suggest that the civilizing of appetite in early modern England was heavily affected by the forces of social mobility as well as the nascent market economy. The Capulets' costly preparation of Juliet's wedding banquet is a showcase of conspicuous consumption which was a structural necessity for the ruling class in Shakespeare's time. Some fifteen years later, the same kinds of foodstuffs are included in a shepherd's shopping list for the sheepshearing festival in Winter's Tale. This is a significant coincidence to prove that food was an important source of emulation and contest among different social classes; and that the rich diet of the upper class gave impetus to social mobility. The Elizabethan subjects, especially among the elite noblemen, were interpellated by the ideology of food that equated the quality of food and the eater's social identity. Faced with bankruptcy as a consequence of his extravagant consumption habit, Bassanio in The Merchant of Venice testifies to the gripping ideology of food onto early modern people, while Poor Tom in King Lear presents a comic parody of the rich people's conspicuous waste. Also in Coriolanus and The Merry Wives of Winsor, Shakespeare uses food as a metaphor for class-motivated social struggles.

Radiographic Comparison of Cranial Tibial Wedge Osteotomy versus Tibial Plateau Leveling Osteotomy: A Cadaveric Study

  • Lee, Jiyoon;Kim, Dongwook;Oh, Hyejong;Lee, Sungin;Choi, Seok Hwa;Kim, Gonhyung
    • Journal of Veterinary Clinics
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    • v.39 no.3
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    • pp.93-99
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    • 2022
  • The present study was performed to compare cranial tibial wedge osteotomy (CTWO) and tibial plateau leveling osteotomy (TPLO) through radiographic evaluation. The experiment was conducted with five cadaver dogs [mean (± SD) weight, 32.9 ± 4.1 kg; mean (± SD) age, 6 ± 2 years; three males and two females] euthanized for reasons unrelated to this study. The cadaver dogs consisted of German Shepherd (n = 3), Belgian Malinois (n = 1), and mixed breed (n = 1). CTWO and TPLO were carried out by the standard surgical method. Radiographic evaluation was performed by comparing several factors, including the flexion and extension angles, the anatomical mechanical axis angle (AMA-angle), tibial length, patellar height measurement using the Labelle-Laurin method, mechanical medial proximal tibial angle (mMPTA), mechanical medial distal tibial angle (mMDTA), and frontal plane alignment (FPA). Both the CTWO and the TPLO groups showed significantly increased flexion angles after surgery. Only the CTWO group had significantly increased extension angle. Although both groups showed significant decreases in the AMA-angle, the mechanical axis moved cranially against the anatomical axis only in the CTWO group. The patellar height was significantly lowered in the CTWO group. No significant differences were found in mMPTA, mMDTA, or FPA. In conclusion, radiographic comparison revealed more changes in CTWO group than in TPLO group.

Quantitative Expression Analysis of Functional Genes in Four Dog Breeds (개의 네 품종에서 기능 유전자들에 대한 정량적 발현 분석)

  • Gim, Jeong-An;Kim, Sang-Hoon;Lee, Hee-Eun;Jeong, Hoim;Nam, Gyu-Hwi;Kim, Min Kyu;Huh, Jae-Won;Choi, Bong-Hwan;Kim, Heui-Soo
    • Journal of Life Science
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    • v.25 no.8
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    • pp.861-869
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    • 2015
  • One of the domesticated species; the dog has been selectively bred for various aims by human. The dog has many breeds, which are artificially selected for specific behaviors and morphologies. Dogs contribute their life to human as working dogs for guide, rescue, detection or etc. Working dogs requires good personality, such as gentleness, robustness and patience for performing their special duty. Many studies have concentrated on finding genetic marker for selecting the high-quality working dog. In this study, we confirmed quantitative expression patterns of eight genes (ABAT; 4-Aminobutyrate Aminotransferase, PLCB1; Phospholipase C, Beta 1, SLC10A4; Solute Carrier Family 10, Member 4, WNT1; Wingless-Type MMTV Integration Site Family, Member 1, BARX2; BarH-Like Homeobox 2, NEUROD6; Neuronal Differentiation 6, SEPT9; Septin 9 and TBR1; T-Box, Brain, 1) among brains tissues from four dog breeds (Beagle, Sapsaree, Shepherd and Jindo), because these genes were expressed and have functions in brain mostly. Specially, BARX2, SEPT9, SLC10A4, TBR1 and WNT1 genes were highly expressed in Beagle and Jindo, and Sapsaree and German Shepherd were vice versa. The biological significance of total genes was estimated by database for annotation, visualization and integrated discovery (DAVID) to determine a different gene ontology (GO) class. In these analyses, we suppose to these eight genes could provide influential information for brain development, and intelligence of organisms. Taken together, these results could provide clues to discover biomarker related to functional traits in brain, and beneficial for selecting superior working dogs.