• 한국수산과학회지
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• 제48권5호
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• pp.789-797
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• 2015

The concentrations of trace metals (As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, and Zn) were determined in 49 surface sediment samples collected in Yeoja and Gangjin Bays on the south coast of Korea, which contain many shellfish farms, in order to assess the contamination level and to understand the spatial distribution of trace metals. The average metal concentrations in the surface sediments of both bays decreased in the order Fe > Mn > Zn > Cr > Pb > Cu > As > Cd > Hg. There were no differences in metal concentrations between shellfish farms and reference sites. The metal concentrations were significantly higher in Gangjin Bay than in Yeoja Bay. Notably, the Cu, Cd, and Hg concentrations were nearly twice as high in Gangjin Bay as in Yeoja Bay. A multivariate analysis showed a strong correlation among Fe, Mn, Zn, Pb, and Cr in the sediments of both bays, implying that the metal concentrations in the sediments in the study area were mainly dependent on natural processes, such as crustal components and diagenesis. Based on the geoaccumulation index and marine sediment quality guidelines (SQGs), the surface sediments in Yeoja and Gangjin Bays are not polluted by trace metals.

• 수산해양기술연구
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• 제33권3호
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• pp.166-172
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• 1997

우리나라 연안 해역의 패류 양식장과 마을어장에 서식하는 불가사리를 효율적으로 구제하기위하여 자루걸레형과 썰매형 구제 기구를 설계, 제작하여 1995년 4월부터 5월까지 경상남도 거제시 장목면 유로리 연안에서 민간 형망어선 (복성호, 4.95톤)을 사용하여 구제 기구별 포획 성능과 예인 거리에 따른 포획 효율을 시험한 결과를 요약하면 다음과 같다. 자루걸레형과 썰매형 구제 기구는 해저의 암초에 걸리지 않고 계속 예인하면서 불가사리를 구제할 수 있었고, 적정 예인 거리는 300~500m였다. 썰매형에 의한 불가사리류의 포획비율은 57%로 자루걸레형의 43%보다 다소 우수 하였으나 어패류의 흔획 포획비율은 자루걸레형보다 21배 정도 높았다. 썰매형은 자갈인 암초가 산재된 마을어장에 적합한 밤년, 자루걸레형은 어패류의 흔획과 유용 패류에 상처를 입힐 수 있는 면적이 작아 패류 양삭장에 효과적이었다.

• 한국수산과학회지
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• 제43권4호
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• pp.298-306
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• 2010

A sanitary survey was conducted to evaluate the water quality and mussel (Mytilus edulis) conditions of two administrative shellfish growing waters: those designated as shellfish growing water for export, and adjacent waters on the east coast of Changseon Island, Namhae, Korea. In all, 1,656 seawater and 166 mussel samples were collected at 46 stations for seawater and five stations for the shellfish from January 2007 to December 2009. Both seawater and mussels were examined for total coliforms and fecal coliforms. The standard plate count and most probable number of Escherichia coli were also determined for the shellfish samples. The range of the geometric means and the estimated 90th percentiles of fecal coliform for seawater samples at each station were <1.8-4.1 MPN/100 mL and <1.8-22.3 MPN/100 mL, respectively. The sanitary conditions for both shellfish growing areas, the designated shellfish growing area and adjacent area in Changseon, met the 'Approved area' criteria of the United States National Shellfish Sanitation Program. The range of E. coli detected in mussels in these areas was <20-500 MPN/100 g; and only 3 of 166 samples exceeded 230 MPN/100 g, the European standard for the consumption of raw bivalves. Therefore, the mussel farms in the Changseon area are classified as 'Class A' according to criteria set in European Community regulations. However, the levels of E. coli in mussels in parts of the designated shellfish growing area and adjacent area exceeded the bacterial limits after rainfall exceeding 45 mm. Further studies are needed to fully define the conditions leading to a temporary closure to harvest after a rainfall event.

• 한국수산과학회지
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• 제54권5호
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• pp.685-690
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• 2021

Harmful shell-boring species of the genus Polydora (Polychaeta: Spionidae) were frequently reported from commercially important mollusk species in Korea, Japan and China. The traditional approach based on the morphological characteristics showed limitations for species discrimination among shell-boring species. Therefore, DNA barcoding was adopted to identify Polydora species using molecular markers. Two Polydora species (P. haswelli and P. hoplura) in abalone shells were reported from our previous molecular phylogenetic study. In this study, we additionally reported the presence of shell-boring Polydora haswelli in commercially sold shellfish. The taxon-specific cox1 marker used in this study successfully allowed the isolation of P. haswelli from cockle Scapharca subcrenata, mussel Mytilus galloprovincialis, oyster Crassostrea gigas and scallop Argopecten irradians. Polydora hoplura was not found in these shellfish species. The genetic variations were found on the intraspecific level of P. haswelli and the same genotype was also detected in different shellfish species. This result can provide information on a new host and accurate parasitic Polydora species. Moreover, this report can be used as the biodiversity data of Polydora species on the invasion and transition of harmful Polydora species in mollusk aquaculture farms.

• 한국패류학회지
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• 제20권1호
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• pp.75-84
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• 2004

Reproductive cycle, the condition index, sex ratio of the Pacific oysters, Crassostrea gigas were investigated by histological and morphometric data. The specimens were collected in the two oyster farms of Geoje and Namhae, Gyeongsangnam-do, Korea, from November 1995 to October 1996. Growth of shell length in two regions was similar, but growth of total weight of the oyster in Namhae was faster than that in Geoje oyster farm. The spawning periods in female and male clams were from July to October in Geoje and from June to October in Namhae oyster farm. Ripe oocytes were approximately 50 m in diameter. The reproductive cycle of in females and males in Geoje and Namhae oyster farms can be divided into five successive stages: early developing, late developing, ripe, partially spawned and spent/inactive. Monthly changes in gonad developmental phases showed somewhat different patterns between female and male clams except for the spawning period. On the whole, however, monthly changes in the gonad developmental phases showed a similar pattern in the same sex. The sex ratios of females to males in Geoje and Namhae oyster farms were not significantly different from a 1:1 sex ratio ($x^2$ = 0.55 (p > 0.05) in Geoje and $x^2$ = 0.27 ( p > 0.05) in Namhae). Artificial induction of maturation by heating of adult oysters (two-year-old) was investigated from 17 January to 18 March in 1996. Maturity at the fixed water temperature group of $20^{\circ}C$ was 80%, it showed the highest maturity of experimental groups cultured for five weeks. The survival (%) of Crassostrea gigas in the raised water temperature experimental groups (15, 20, $25^{\circ}C$) were over 98.5%, as similar to the control group (100%). But, the survival of C. gigas in the fixed water temperature experimental groups (15, 20, 25, $30^{\circ}C$) were decreased with the increase of the water temperatures. In the fixed water temperature experimental group of $30^{\circ}C$, the survival was 51.1%. Base on these results, the fixed water temperature of $20^{\circ}C$ was the best condition for artificial induction of sexual maturation.

• 환경생물
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• 제40권4호
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• pp.374-386
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• 2022

Phytoplankton communities, with emphasis on picoplankton and nanoplankton, were investigated in Gamak Bay, South Korea, where freshwater input and coastal water intrusion shape ecosystem functions. Shellfish farms and fish farms are located in the inner bay and outer bay, respectively, and tides translocate uneaten food and urine production from aquaculture farms toward the inner bay. Water masses were distinctly different based on a significantly different density between the surface and bottom layer and among three water masses, including the inner bay, outer bay, and Yeosu Harbor. Phytoplankton communities were quantified using flow cytometry and size-fractionated chlorophyll-a (chl-a) was measured. Salinity was a principal variable separating phytoplankton communities between the surface and bottom layer, whereas Si(OH)₄ controlled the communities in the inner bay, and NH₄⁺ and PO₄^3- governed the outer bay communities. While phycocyanin-containing (PC) cyanobacteria dominated in the outer bay, phycoerythrin-containing (PE) cyanobacteria dominance occurred with cryptophyte dominance, indicating that nutrients affected the distribution of pico- and nanoplankton and that cryptophytes potentially relied on a mixotrophic mode by feeding on PE cyanobacteria. Interestingly, picoeukaryotes and eukaryotes larger than 10 ㎛ were mostly responsible for the ecological niche in the western region of the bay. Given that chl-a levels have historically declined, our study highlights the potential importance of increased small phytoplankton in Gamak Bay. Particularly, we urge an examination of the ecological role of small phytoplankton in the food supply of cultivated marine organisms.

• 한국수산과학회지
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• 제15권1호
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• pp.35-41
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• 1982

진해만 패류양식장의 어장가치를 진단하는 기초자료를 준비코자 양식장 저이의 오염정도를 구명하고 아울러 저이오염원의 기원을 파악코자 1981년 하계 중 만내 87개의 조사지점에서 저직성분을 조사하였다. 1. 저는 만 전체에 걸쳐 이질로 덮혀 있었다. 2. 페오파이친색소양은 $7.6-48.2{\mu}g/g$건이, COD양은 9.7-38.5 mg/g건이, 강열감양은 $8.1-14.2\%$ 그리고 유화물양은 0.1mg/g 이하로부터1.07mg/g 까지 이었다. 3. 상기 4개 항목의 양의분포는 다함께, 진해만 입구부인 잠도근처에서 견내양쪽으로 갈수록 많았으며, 저이오염도는 패류양식장이 많은 가조도 서부해역이 가장 높았다. 4. 가조도 서부해역의 대부분이 COD와 유화물양에 있어 부영양역의 상한치를 상인하고 있었다.5. 이오염의 주원은 양식패와 부착동물 배비물인 유기물의 축적이라 추측되었다.

• 화약ㆍ발파
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• 제31권1호
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• pp.11-22
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• 2013

본 연구에서는 암반의 굴착(발파)공사 등으로 인하여 발생되는 지반진동과 어패류 양식장 등의 수중 배경소음도 및 실제 발파작업시에 발생하는 수중 대상소음도의 척도를 비교 분석하고, 회귀분석에 의한 지반진동추정식과 수중 대상소음 간의 상관식을 도출하여 굴착공사시 수중소음도의 발생정도를 사전 예측하고자 하였으며, 나아가 수중소음 관리기준의 정립을 위한 첫걸음으로 본 연구를 시작하게 되었다. 그동안 실험을 진행하기 전에 상황조건을 달리한 어패류 양식장에 대해 배경소음도를 측정하여 본 결과 양식장마다 각각의 환경적 특성에 따라 배경소음 수준정도가 다양하게 나타나고 있고, 수중소음을 유발하는 지반진동을 실측하여 지반진동과 수중 대상소음 간의 상호 관계식을 도출할 수 있었다. 따라서 상관식에 의해 각종 건설공사에서 수중소음 유발정도를 사전에 예측하여 당해 실정에 적합한 관리(허용)기준을 설정, 설계 시공 등에 적용할 수 있으므로 과거의 기술적, 공학적 논리의 빈약한 기술전개로 인한 어패류 피해의 경제적 손실을 사전에 예방할 수 있고, 수중생물체에 대한 안전성을 확보한 가운데 원만한 건설공사를 진행할 수 있다는 가능성을 발견할 수 있었다.

• 한국수산과학회지
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• 제38권6호
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• pp.372-378
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• 2005

An analysis method of tetracyclines far fish and shellfish products containing large amount of low molecular materials and pigments was established. The recovery of the established analysis method for four tetracycline samples was $72-100\%$ and higher than other methods reported. Especially, proposed sample treatment protocol was shown to be effective for the removal of low molecular materials and pigments that tend to interfere with accurate analysis. The detection limit of oxytetracycline (OTC) and tetracycline (TC) from the sample was 0.02 ppm, and the detection limit of chlortetracycline (CTC) and doxycycline (DC) from the sample was 0.1ppm, To examine the efficiency of the established method and identify tetracycline usage in fish farms, tetracycline group antibiotics in the flounder being cultured was monitored. The improved method can be used for fish and shellfish products effectively and all surveyed fish farms have used tetracycline all the year round. The proposed method was adopted as official method for fishery products by Korean Food and Drug Administration in 2003 and it is being used by regulatory authority as National Fishery Products Quality Inspection Service.

• 한국수산과학회지
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• 제55권5호
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• pp.505-513
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• 2022

In this study, 143 strains of Enterococcus spp. were isolated from inland pollution sources near shellfish farms on the west coast of South Korea. Not all isolated Enterococcus spp. strains possessed vancomycin resistance genes (VanA and VanB). However, since vancomycin-resistance Enterococcus (VRE) have been detected not only in the clinical field but also out in the world, it is possible that the VRE gene may be transferred to other bacterial strains commonly found in coastal waters where seafood is produced. It is important to monitor trends in the appearance of VRE. In addition, antimicrobial resistance patterns of isolates were examined in this study. Overall antimicrobial resistance rates were high: ciprofloxacin (32.2% of isolates resistant), chloramphenicol (30.8%), quinupristin/dalfopristin (19.6%), and tylosin (15.4%). Eight E. faecium strains (6.2%), out of the 129 strains assessed, showed multidrug resistance. All multidrug-resistant E. faecium showed resistance to erythromycin, quinupristin/dalfopristin, tetracycline, and tylosin, in all 14 strains. All multidrug-resistant E. faecalis showed resistance to erythromycin, quinupristin/dalfopristin, tetracycline, and tylosin. Both multidrug-resistant E. faecium and multidrug-resistant E. faecalis showed common resistance to erythromycin, quinupristin/dalfopristin, tetracycline, and tylosin.