• 한국축산식품학회지
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• 제34권2호
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• pp.245-251
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• 2014

The aim of this study was to estimate the shelf life of butter and cheese products, with shelf life being a guide used to determine the storage period of food before deterioration. Butter and cheese samples stored at $10^{\circ}C$ and $15^{\circ}C$ had a shelf life of 221 d, while those stored at $25^{\circ}C$ and $35^{\circ}C$ had a shelf life of 109 d. Quality changes, including total cell count, coliform counts, Listeria monocytogenes counts, acid value, moisture content, pH, acidity and overall sensory evaluation, were monitored. In order to pass the overall sensory evaluation, a quality score of 5 points on a 9-point scale was required. For other quality criteria, legal quality limits were established based on the "Process Criteria and Ingredient Standard of Livestock Products" by the Animal, Plant and Fisheries Quarantine and Inspection Agency (Republic of Korea). The nonlegal quality limit was estimated by regression analysis between non-quality criteria (y) and overall sensory evaluation (x). The shelf life was estimated based on the number of days that the product passed the quality limit of the quality criteria. The shelf life of samples stored at $10^{\circ}C$, $15^{\circ}C$, $25^{\circ}C$ and $35^{\circ}C$ was 21.94, 17.18, 6.10 and 0.58 mon, respectively, for butter and 10.81, 9.47, 4.64 and 0.20 mon, respectively, for cheese.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1243-1252
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• 2009

User-friendly software was developed to determine the shelf-life of perishable Korean seasoned side dishes in real time based on growth models of spoilage and pathogenic microorganisms. In the program algorithm, the primary spoilage and fastest-growing pathogenic organisms are selected according to the product characteristics, and their growth is simulated based on the previously monitored or recorded temperature history. To predict the growth of spoilage organisms with confidence limits, kinetic models for aerobic bacteria or molds/yeasts from published works are used. Growth models of pathogenic bacteria were obtained from the literature or derived with regression of their growth rate data estimated from established software packages. These models are also used to check whether the risk of pathogenic bacterial growth exceeds that of food spoilage organisms. Many example simulations showed that the shelf-lives of the examined foods are predominantly limited by the growth of spoilage organism rather than by pathogenic bacterial growth.

• 한국축산식품학회지
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• 제18권2호
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• pp.125-131
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• 1998

This study were carried out for investigation of microbiological characteristics, and establishment of shelf-life of Korean vacuum packaged chilled pork hams for export. The samples were stored at 0$\pm$1$^{\circ}C$ (A and B companies) and 2$\times$1$^{\circ}C$ (C and D companies). In the analysis of microorganisms, the numbers of total plate counts were 3.72$\times$103~1.15$\times$104CFU / $\textrm{cm}^2$ at initial time. After 30 days, total plate counts were over 1$\times$106CFU / $\textrm{cm}^2$ in samples. Anaerobic counts were over 1$\times$106CFU / $\textrm{cm}^2$ in samples excluded one of A company after 30 days. Using regression equations, the estimation of shelf life were made : 32~37 days in storing at $0^{\circ}C$ and 27 days at 2$^{\circ}C$. In this case, the determination coefficients were 0.8624~0.9515 and 0.8236~0.8565, respectively.

• 식품과학과 산업
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• 제42권1호
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• pp.71-79
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• 2009

• 한국식품과학회지
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• 제27권5호
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• pp.703-707
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• 1995

조리가공후 열처리하여 미생물학적 변패의 가능성을 배제한 식품의 저장중 일어나는 이화학적, 관능적 변화를 주성분분석을 이용하여 품질저하를 평가하였다. 제조된 식품을 20, 30 및 $40^{\circ}C$에서 저장하면서, pH, 산도, 색도 및 3개의 관능특성 변화 등 9개의 항목을 측정하였다. 전체적인 기호도는 시간이 경과함에 따라 서서히 떨어져 시료의 관능적 품질수명은 $40^{\circ}C$의 경우는 1.5개월, $20^{\circ}C$ 및 $30^{\circ}C$의 경우는 3개월 수준으로 나타났다. 그러나 저장온도의 상승에 따른 이화학적 품질의 변화속도는 일정한 경향이 없었다. 주성분분석을 이용하여 저장중의 품질 변화를 평가한 결과, 9개의 측정항목에 대하여 제1주성분은 49.6%, 제2주성분은 28.8%를 설명할 수 있었다. 제1주성분점수의 변화는 유의적이었으며, 변화의 기울기가 20, 30 및 $40^{\circ}C$에서 각각 -1.07, -1.33 및 -1.82로서, 제1주성분점수의 변화는 저장온도가 $10^{\circ}C$씩 증가함에 따라 약 1.30배씩 증가하여, 기호도검사에 의한 $20^{\circ}C$ 및 $40^{\circ}C$의 저장수명 변화와 일치하였다. 따라서 주성분 분석을 이용하여 식품의 저장중 품질변화를 평가하고, 저장수명을 예측하며, 품질에 영향을 미치는 지표의 선정이 가능할 것으로 사료되었다.

• 한국축산식품학회지
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• 제38권4호
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• pp.664-678
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• 2018

This study was carried out to establish shelf life for pork cutlet of ground meat and pork lard by using various quality indicators and to understand how quality changes in these products are accelerated by temperature. The samples were selected and purchased from markets in Korea, and the chosen quality indicators were total aerobic counts and coliform group in microbiological analyses, thiobarbituric acid reactive substances assay, volatile basic nitrogen, pH, acid value, and peroxide value in physical chemical analyses, and sensory evaluation. The pork cutlet samples were stored at $-18^{\circ}C$, $-6^{\circ}C$, and $-1^{\circ}C$, whereas pork lard samples were stored at $10^{\circ}C$, $25^{\circ}C$, $35^{\circ}C$, and $45^{\circ}C$. These temperature conditions were set to real distribution conditions. The samples were then analyzed using various models including of reaction orders, arrhenius equation, and $Q_{10}$ value. The quality limits for each sample were calculated, and shelf life was estimated. The results of this experiment highlighted the importance of temperature control during the distribution process of these products and revealed that temperature is a useful parameter for the establishment of a basic database for shelf life.

• 한국포장학회지
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• 제26권3호
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• pp.165-171
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• 2020

유통과정에서 균질한 저온을 유지하는 것은 식품의 유통기한을 결정하는 가장 중요한 요소 중 하나인 미생물 증식을 억제하는 핵심 기술이다. 신선수산물의 전과정에서 정확하고 적절한 온도관리가 보장되었을 때 높은 품질의 신선 제품을 소비자에게 공급할 수 있다. 이 논문은 슈퍼칠링 신선수산식품의 상업성을 확인하기 위하여 포장된 신선 광어(Paralichthys Olivaceus) 의 유통기한을 연구한 것이다. 유통기한 측정을 위하여 중량, 대장균 등 세균수, pH, 관능검사, 색상, 휘발성 염기질소 등이 조사되었다. 연구결과, 휘발성염기질소를 기준으로 슈퍼칠링 온도(0±1℃)에서의 유통기한이 6일인데 비하여 일반 냉장온도 (8±2℃)의 경우 1일에 불과하여 슈퍼칠링 유통의 가능성을 보여주었다. 상업화 추진을 위해서는 소비자 신뢰도를 높이고 경제성을 향상시킬 수 있도록 슈퍼칠링 기법과 포장시스템에 대한 추가적인 포장방법 연구가 필요한 것으로 사료된다.

• 한국포장학회:학술대회논문집
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• 한국포장학회 2006년도 정기총회 및 추계학술발표대회
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• pp.115-126
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• 2006

• 한국포장학회지
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• 제23권2호
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• pp.75-81
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• 2017

반찬이나 간식으로 이용되는 소비성이 높은 튀김어묵에 대해서 진공, 60% $N_2/40%$ $CO_2$, 100% $N_2$ 및 100% $CO_2$의 변형기체포장조건을 사용하여 저장성 향상과 유통기한 연장을 얻을 수 있는 가능성을 검토하였다. $5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$의 온도 조건에서 저장하면서 품질변화를 측정한 결과 주요 품질열화요소로서 호기성 세균 증식임이 인지되었다. 호기성 세균수 성장을 Huang의 수학적 모델을 통하여 표현하고, 포장 및 저장온도조건에 따른 모델 parameter를 구하였고, 이를 사용하여 한계 미생물 수준에 도달하는 기간으로서 포장 및 온도 조건에 따른 저장유통기한을 설정하여 제시하였다. Arrhenius 방정식으로 표현된 미생물 성장의 온도의존성에서는 유도기의 역수에 대해서 활성화 에너지 139.4 kJ/mol, 성장속도에 대한 활성화 에너지는 41.8 kJ/mol로 얻어졌다. 변형기체포장의 효과에서, $10^{\circ}C$에서 100% 이산화탄소 포장이 호기성 세균 증식을 효과적으로 억제하여 함기포장에 비하여 약 2배의 유통기한을 확보하여 저장성을 향상시킬 수 있었다. 고이산화탄소 상태에서 저온의 온도 조건을 활용하여 저장유통기한을 연장할 수 있을 것으로 확인되었다.

• KSBB Journal
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• 제13권5호
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• pp.502-510
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• 1998

Stabilization of antibody, which is specific to Salmonella typhimurium antigens, present in dry states on membranes was accomplished, and its shelf-life, i.e., duration for maintaining minimum 90% of the initial activity, under optimal conditions was determined. To prepare two major components of an immuno-strip, the antibody was not only immobilized on nitrocellulose membrane surfaces but also placed within the pores of glass fiber membrane after conjugating it with old colloids as signal generator. Among potential stabilizers of the immuno-components, a disaccharide, trehalose, showed a significant protection effect of immunoglobulin structure from thermal energy. Optimal concentrations of trehalose for the respective component were significantly different (8-fold higher for the antibody-gold conjugate than for the immobilized antibody), which probably resulted from distinct densities and configurations of antibody present on the membranes. An additional requirement for the gold conjugate was freeze-drying of this substance such that the conjugate can be readily resolubilized upon contact with aqueous medium. By using the components prepared under optimal conditions, immuno-strips were constructed and exposed to thermal energy. Signals with less than 10% decrease in the intensity were maintained for approximately 21 days at 60$^{\circ}C$. Compared to previous reports, this result represented a 2-year shelf-life at room temperature. it was, however, two times longer if determined from thermal acceleration tests based on the theory of inactivation rate of protein. Such discrepancy between the two estimates could be mainly attributed to errors in accurately controlling temperatures and also to changes in the physical properties of membranes due to a high thermal energy.