• Journal of Microbiology and Biotechnology
• /
• v.16 no.11
• /
• pp.1690-1698
• /
• 2006

In order to investigate the effect of dissolved oxygen (DO) level on AVM $B_{1a}$ production by a high yielding mutant of Streptomyces avermitilis, five sets of bioreactor cultures were performed under variously controlled DO levels. Using an online computer control system, the agitation speed and aeration rate were automatically controlled in an adaptive manner, responding timely to the oxygen requirement of the producer microorganism. In the two cultures of DO limitation, the onset of AVM $B_{1a}$ biosynthesis was observed to casually coincide with the fermentation time when oxygen-limited conditions were overcome by the producing microorganism. In contrast, this phenomenon did not occur in the parallel fermentations with DO levels controlled at around 30% and 40% throughout the entire fermentation period, showing an almost growth-associated mode of AVM $B_{1a}$ production: AVM $B_{1a}$ biosynthesis under the environments of high DO levels started much earlier than the corresponding oxygen-limited cultures, leading to a significant enhancement of AVM $B_{1a}$ production during the exponential stage. Consequently, approximately 6-fold and 9-fold increases in the final AVM $B_{1a}$ production were obtained in 30% and 40% DO-controlled fermentations, respectively, especially when compared with the culture of severe DO limitation (the culture with 0% DO level during the exponential phase). The production yield ($Y_{p/x}$), volumetric production rate (Qp), and specific production rate (${\bar{q}}_p$) of the 40% DO-controlled culture were observed to be 14%, 15%, and 15% higher, respectively, than those of the parallel cultures that were performed under an excessive agitation speed (350 rpm) and aeration rate (1 vvm) to maintain sufficiently high DO levels throughout the entire fermentation period. These results suggest that high shear damage of the high-yielding strain due to an excessive agitation speed is the primary reason for the reduction of the AVM $B_{1a}$ biosynthetic capability of the producer. As for the cell growth, exponential growth patterns during the initial 3 days were observed in the fermentations of sufficient DO levels, whereas almost linear patterns of cell growth were observed in the other two cultures of DO limitation during the identical period, resulting in apparently lower amounts of DCW. These results led us to conclude that maintenance of optimum DO levels, but not too high to cause potential shear damage on the producer, was crucial not only for the cell growth, but also for the enhanced production of AVM $B_{1a}$ by the filamentous mycelial cells of Streptomyces avermitilis.

• Journal of Korean Society of Steel Construction
• /
• v.11 no.1 s.38
• /
• pp.33-42
• /
• 1999

In the present study, crack initiation criteria, static failure and tensile mode fatigue behavior for a rail steel are evaluated to assure the railway vehicle's safety. The transverse fissure, which is the most critical damage in the rail, is initiated by the maximum shear stress and its location is subsurface. In addition, the possibility of transition from the shear mode to the mixed mode increases with increasing the length of subsurface crack. Because of the brittleness by the welding, the fracture toughness of the welded part is lower than of the base metal. For low ${\Delta}K$, the stage II fatigue crack growth rates of the welded part is slower than of the base metal but, for high ${\Delta}K$, this different behavior for fatigue crack growth rate is nearly diminished. These trends are more remarkable for low stress ratio, R=0.1. It is believed that this behavior is caused by the change of the microstructure which that of the welded part is coarser than of base metal.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2008.11a
• /
• pp.317-320
• /
• 2008

In recent year, the ultra high strength concrete has highly increased and been used in many parts of the world. However, the viscosity of the ultra high strength concrete is high because of a low water to binder ratio (w/b). So that in this pater, the shear stress and the shear strain rate are directly measured by the viscometer in order to estimate the rheological properties of the ultra high strength concrete and a linear regression analysis was carried out to determine the plastic viscosity and the yield stress as slope. According to the test results, the yield stress and plastic viscosity are correlated to slump-flow, V-funnel flow time, O-lot flow time

• Journal of Soil and Groundwater Environment
• /
• v.6 no.1
• /
• pp.53-62
• /
• 2001

Effects of surface defect on thermal stability and stress crack resistance of high density polyethylene geomembranes in environmental conditions were examined by comparing the mechanical properties, chemical resistance and failure times of geomembranes between defective cases under different temperatures. Artificial surface defects were added to the surface of geomembranes by scratch apparatus designed specially. The number of surface defects was increased with the smaller size of scratch induced particles, and the more scratch addition numbers at the shear rate of scratch induced mechanism, 100mm/min. The tensile strength were decreased but the tensile strain was increased with the above conditions. In chemical resistance of defective geomembranes, the tensile strength were decreased but the tensile strain was increased with the longer immersion period and the higher temperature under the same scratch induced conditions. Finally, failure times of defective geomembranes by ESCR test were shifted to the shorter time ranges by increasing temperatures.

• Journal of Ocean Engineering and Technology
• /
• v.23 no.5
• /
• pp.45-53
• /
• 2009

In this study, Finite Element Analysis (FEA) was used to decide three kinds of material property of vibration proof rubber with the unique characteristic of non-linear and large deformation. As well, three types of hardness (Hs 50, 55, 60) were compared with the result of fatigue tests, fatigue life was able to be predicted. The request for fatigue life becomes strict more and more as increasing stress under conditions like a compaction, high load and high temperature for parts because it is main characteristics of rubber mount for automotive. Regarding to the fatigue life under dynamic deformation condition, it can be predicted as checking forced deformation extends and its frequency and its strain-life curve. As for material property tests of uniaxial tension test, uniaxial compression test, pure shear test, Ogden model was used for FEA by observing relations between stress and strain's rate as curve fitting. As a result of FEA, fatigue life for rubber mount was predicted and accorded well with the experimental data of fatigue test with hourglass specimens. In addition, its property of the predictable fatigue life method suggested in this study was accorded well with the experimental data by comparing the predicted fatigue life of FEA with the result of fatigue test for rubber component of engine rubber mount.

• Steel and Composite Structures
• /
• v.22 no.1
• /
• pp.141-159
• /
• 2016

Commonly in steel frames, steel beam and concrete slab are connected together by shear keys to work as a unit member which is called composite beam. When a composite beam is subjected to positive bending, flexural strength and stiffness of the beam can be increased due to "composite action". At the same time despite these advantages, composite action increases the strain at the beam bottom flange and it might affect beam plastic rotation capacity. This paper presents results of study on the rotation capacity of composite beam connected to Rectangular Hollow Section (RHS) column in the steel moment resisting frame buildings. Due to out-of-plane deformation of column flange, moment transfer efficiency of web connection is reduced and this results in reduction of beam plastic rotation capacity. In order to investigate the effects of width-to-thickness ratio (B/t) of RHS column on the rotation capacity of composite beam, cyclic loading tests were conducted on three full scale beam-to-column subassemblies. Detailed study on the different steel beam damages and concrete slab damages are presented. Experimental data showed the importance of this parameter of RHS column on the seismic behavior of composite beams. It is found that occurrence of severe concrete bearing crush at the face of RHS column of specimen with smaller width-to-thickness ratio resulted in considerable reduction on the rate of strain increase in the bottom flange. This behavior resulted in considerable improvement of rotation capacity of this specimen compared with composite and even bare steel beam connected to the RHS column with larger width-to-thickness ratio.

• Structural Engineering and Mechanics
• /
• v.12 no.5
• /
• pp.507-526
• /
• 2001

In standard finite element algorithms, the local stability conditions are not accounted for in the formulation of the tangent stiffness matrix. As a result, the loss of the local stability is not adequately related to the onset of the global instability. The phenomenon typically arises with material-type localizations, such as shear bands and plastic hinges. This paper addresses the problem in the context of the planar, finite-strain, rate-independent, materially non-linear beam theory, although the proposed technology is in principle not limited to beam structures. A weak formulation of Reissner's finite-strain beam theory is first presented, where the pseudocurvature of the deformed axis is the only unknown function. We further derive the local stability conditions for the large deformation case, and suggest various possible combinations of the interpolation and numerical integration schemes that trigger the simultaneous loss of the local and global instabilities of a statically determined beam. For practical applications, we advice on a procedure that uses a special numerical integration rule, where interpolation nodes and integration points are equal in number, but not in locations, except for the point of the local instability, where the interpolation node and the integration point coalesce. Provided that the point of instability is an end-point of the beam-a condition often met in engineering practice-the procedure simplifies substantially; one of such algorithms uses the combination of the Lagrangian interpolation and Lobatto's integration. The present paper uses the Galerkin finite element discretization, but a conceptually similar technology could be extended to other discretization methods.

• Korean Journal of Materials Research
• /
• v.29 no.10
• /
• pp.647-655
• /
• 2019

In order to analyze the effect of hot asymmetric rolling on the microstructure and texture of aluminum alloy and to investigate the effect of the texture on the formability and plastic anisotropy of aluminum alloy, aluminum 6061 alloy is asymmetrically rolled at room temperature, $200^{\circ}C$, $350^{\circ}C$, and $500^{\circ}C$, and the results are compared with symmetrically rolled results. In the case of asymmetric rolling, the equivalent strain (${\varepsilon}_{eq}$) is greatest in the upper roll part where the rotational speed of the roll is high and increases with increasing rolling temperature. The increase rate of the mean misorientation angle with increasing temperature is larger than that during symmetrical rolling, and dynamic recrystallization occurs the most when asymmetrical rolling is performed at $500^{\circ}C$. In the case of hot symmetric rolling, the {001}<110> rotated cube orientation mainly develops, but in the case of hot asymmetric rolling, the {111}<110> orientation develops along with the {001}<100> cube orientation. The hot asymmetric rolling improves the formability (${\bar{r}}$) of the aluminum 6061 alloy to 0.9 and reduces the plastic anisotropy (${\Delta}r$) to near zero due to the {111}<110> shear orientation that develops by asymmetric rolling.

• Earthquakes and Structures
• /
• v.26 no.3
• /
• pp.239-249
• /
• 2024

A new layered shear continuum model box was developed to address the dynamic response issues of buried oil and gas pipelines under multi-point excitation. Vibration table tests were conducted to investigate the seismic response of buried pipelines and the surrounding soil under longitudinal multi-point excitation. A nonlinear model of the pipeline-soil interaction was established using ABAQUS finite element software for simulation and analysis. The seismic response characteristics of the pipeline and soil under longitudinal multi-point excitation were clarified through vibration table tests and simulation. The results showed good consistency between the simulation and tests. The acceleration of the soil and pipeline exhibited amplification effects at loading levels of 0.1 g and 0.2 g, which significantly reduced at loading levels of 0.4 g and 0.62 g. The peak acceleration increased with increasing loading levels, and the peak frequency was in the low-frequency range of 0 Hz to 10 Hz. The amplitude in the frequency range of 10 Hz to 50 Hz showed a significant decreasing trend. The displacement peak curve of the soil increased with the loading level, and the nonlinearity of the soil resulted in a slower growth rate of displacement. The strain curve of the pipeline exhibited a parabolic shape, with the strain in the middle of the pipeline about 3 to 3.5 times larger than that on both sides. This study provides an effective theoretical basis and test basis for improving the seismic resistance of buried oil and gas pipelines.

• Computers and Concrete
• /
• v.15 no.6
• /
• pp.903-919
• /
• 2015

The flow of freshly mixed cement-based material shows thixotropy, which implies some difficulties on robust measurement of its rheological properties: The flow curve of thixotropic materials depends on the used protocol. For examples, higher viscosity is obtained when the rate of shear strain is more quickly increased. Even though precise measurement and modelling of the concrete rheology needs to consider the thixotropic effect, engineers in the concrete field prefer considering as a non-thixotropic Herschel-Bulkley fluid, even more simply Bingham fluid. That is due to robustness of the measurement and application in casting process. In the aspect of simplification, this papers attempts to mimic the thixoropic flow by the non-thixotropic Herschel-Bulkley model. Disregarding the thixotropy of cement based materials allows us to adopt the rheological concept in the field. An optimized protocol to measure the Bingham parameters was finally found based on the accuracy and reproducibility test of cement paste samples, which minimizes the error of simulation stemming from the assumption of non-thixotropy.