• Journal of Plant Biotechnology
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• 제34권4호
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• pp.355-361
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• 2007

Cotyledons of perilla6 were cultured on MS medium containing 0.5 mg/l NAA and 0.5 mg/l BA for 7 weeks. The activity of perilla peroxidase was observed to increase following culture stages as assessed by peroxidase assay. A peroxidase (POD) was purified from perilla tissue cultured on MS medium for 7 weeks. The peroxidase was purified using ion exchange and gel nitration chromatography. The perilla peroxidase had a molecular mass of 30 kDa by SDS-PAGE. We showed that the N-terminal amino acid sequence of this protein shared 67% identity with the tea peroxidase. As indicated by SDS-PAGE, the banding pattern of the 30 kDa polypeptide present in total soluble protein from perilla tissue was increased following culture stages. Immunoblot analysis indicated that perilla peroxidase protein appeared after 3 weeks of perilla tissue culture, and continued to increase with extended duration of tissue culture for at least 7 weeks.

• Ocean and Polar Research
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• 제29권2호
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• pp.165-185
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• 2007

Exploitation of transboundary oil and gas on continental shelves may cause conflicts between or among States concerned due to the physical character of these resources. As oil and gas are fluid, exploitation of such a transboundary oil field by one side may affect other parties in other jurisdictions. However, there is no universal international legal regime on the issue. This article tries to find the international legal regime governing such resources through analysing UN Assembly's resolutions, UNCLOS, international judicial opinions, bilateral agreements and ILC activities relating to transboundary natural resources. As a result of this study, it seems that each coastal State has an inherent sovereign right on its part of the transboundary oil and gas deposit, but this right is not unlimited. Each state involved with the deposit has a duty to cooperate with other states-through information exchange, consultation, and negotiation. Furthermore, the state has an obligation to refrain from unilateral action when there is a possibility of causing irrevocable damage to the interests of the other states.

• Interdisciplinary Bio Central
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• 제3권1호
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• pp.2.1-2.4
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• 2011

Arabidopsis is well-known to the world's plant research community as a model plant. Many significant resources and innovative research tools, as well as large bodies of genomic information, have been created and shared by the research community, partly explaining why so many researchers use this small plant for their research. The genome sequence of Arabidopsis was fully characterized by the end of the $20^{th}$ century. Soon afterwards, the Arabidopsis research community began a 10-year international project on the functional genomics of the species. In 2001, at the beginning of the project, the RIKEN BioResource Center (BRC) started its Arabidopsis resource project. The following year, the National BioResource Project was launched, funded by the Japanese government, and the RIKEN BRC was chosen as a core facility for Arabidopsis resource. Seeds of RIKEN Arabidopsis transposon-tagged mutant lines, activation-tagged lines, full-length cDNA over-expresser lines, and natural accessions, as well as RIKEN Arabidopsis full-length cDNA clones and T87 cells, are preserved at RIKEN BRC and distributed around the world. The major resources provided to the research community have been full-length cDNA clones and insertion mutants that are suitable for use in reverse-genetics studies. This paper provides an overview of the Arabidopsis resources made available by RIKEN BRC and examples of research that has been done by users and developers of these resources.

• 한국전통조경학회지
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• 제33권3호
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• pp.95-102
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• 2015

본 연구는 전통마을 보전과 개발에 적용할 수 있는 자원 평가기준 및 한국과 중국 전문가들 사이에서 나타나는 전통마을 자원 중요도 차이점을 도출하기 위하여 두 나라 관련 전문가를 대상으로 전통마을 자원에 대하여 AHP기반의 설문조사를 실시하였다. 평가항목은 전통마을 자원의 중요도 및 우선순위 도출을 목표로 3계층으로 구분하였다. 분석결과 Level 1에서는 자연자원, Lavel 2에서는 환경자원, 역사자원, 시설자원, Lavel 3에서는 공기, 지형, 전통주택, 농업경관, 공동생활시설, 도농교류활동, 씨족활동 등의 인자가 중요한 것으로 나타났으며, 한국과 중국 두 그룹에서 가장 중요하다고 평가한 인자는 동일하게 나타났다. 평가항목별 종합적인 중요도는 공기, 수질, 소음, 전통주택, 지형, 공동생활시설 등 인자가 한국과 중국 두 그룹에서 상대적으로 중요한 것으로 나타났다. 즉 전통마을은 문화자원요소들을 보전해야 한다는 필연성을 의무처럼 가지고 있지만 질 좋은 자연환경의 관리가 우선적으로 필요하다. 본 연구의 결과는 한국과 중국 전통마을 자원의 중요도에 대한 비교가 되었을 뿐만 아니라 전통마을의 보전과 관리를 위한 자원별 순위와 배점을 산출함으로써 두 나라 전통마을 자원 평가 시 정량적인 데이터 결과를 각각 적용할 수 있는 방안이 될 것으로 기대한다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.477-481
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• 2007

Korean Ogol chicken has been registered as a natural monument in Korea and regarded as a valuable genetic resource for the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis and calculation of genetic diversities have been performed using mitochondrial DNA (mtDNA) sequence variations. A total of 31 Korean Ogol chicken was grouped into four haplotypes and the large haplotype was represented in 12 individuals. The unrooted neighbor-joining tree indicates that the Korean Ogol chicken shared three (A to C) major chicken lineages representing the high genetic variability of this breed. These results can be used for making the breeding and conservation strategies for the Korean Ogol chicken.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.37-53
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• 2001

Two regions of mtDNA genome, cytochrome oxidase subunit I (COI) and 165 ribosomal RNA (165 rRNA) genes, were sequenced for 15 species of the long-horned beetle belonging to four subfamilies and geographic samples of mulberry longicorn beetle, Apriona germari, from two localities in Korea. Ten samples of A. germari collected from Suwon and Busan revealed three COI haplotypes ranging in nucleotide divergence of 0.3% to 0.5%, and the two populations shared one common COI haplotype (80%). The sequence divergence among 15 species of the long-horned beetle was much higher in COI gene (12.3%∼39.4%) than 16S rRNA gene (7.2% to 23.1), and the maximum value in the COI gene is exceptional compared with other relevant studies, including that of Coleoptera. The greatly increased divergence in the COI gene, in facto was stemmed from a peculiar sequence of Prionus insularis belonging to Prioninne, divergence of which ranges from 31.2% to 39.3% from other species. We discussed possible reason of the divergence in this species. Due to the abnormality of COI gene divergence, decrease in phylogenetic signal was severe in COI nucleotide and, subsequently, the converted amino acid sequences, rendering us to put more confidence on the 16S5 rRNA gene data. Although the molecular phylogeny confidently supports the monophyletic origin of Lepturinae, the presence of discrepancy between molecular data and traditional taxonomic views also is a testable hyothesis. One such discrepancy includes taxonomic position of Sophronica obrioides and Theophilea cylindricollis belonging to Lamiinae.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2018년도 추계학술대회
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• pp.474-476
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• 2018

현재 기후 변화를 비롯한 여러 자연재해 및 여러 재해로 인해 막대한 손실을 가져오고 있으며, 이러한 자연재해는 원천적 차단은 어렵다. 그러나 이런 재난 현장의 정보를 실시간으로 수집하여 빠른 시간내에 피해를 줄이기 위한 방안으로 본 연구에서는 ICT 기술을 접목하여 대응전략을 구사하며, 미래 유비쿼터스 사회를 대비하기 위한 USN 기술을 응용한 모델이 필요하여 제안하고자 한다.

• 한국육종학회지
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• 제42권1호
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• pp.11-22
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• 2010

The population structure of a domesticated species is influenced by the natural history of the populations of its pre-domesticated ancestors, as well as by the breeding system and complexity of breeding practices implemented by humans. In the genetic and population structure analysis of 122 South Asia collections using 29 simple sequence repeat (SSR) markers, 362 alleles were detected, with an average of 12.5 per locus. The average expected heterozygosity and polymorphism information content (PIC) for each SSR locus were 0.74 and 0.72,respectively. The model-based structure analysis revealed the presence of three clusters with the 91.8% (shared > 75%) membership, with 8.2% showing admixture. The genetic distances of Clusters 1-3 were 0.55, 0.56, and 0.68, respectively. Polymorphic information content followed the same trend (Cluster 3 had the highest value and Cluster 1 had smallest value), with genetic distances for each cluster of 0.52, 0.52, and 0.65, respectively. This result could be used for supporting rice breeding programs in South Asia countries.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.104-113
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• 2001

Smallholder dairy production systems in developing countries are discussed with reference to type of systems, their characteristics, potential, and opportunities for improvement. Three types of dairy systems are identified and described: smallholder systems, smallholder cooperative dairy production systems, and intensive dairy production systems. The first two systems are by far the most important, and are associated with increasing intensification. Buffaloes are especially important in South Asia, but elsewhere dairy production mainly involves Holstein-Friesian cross-bred cattle. Dairy goats are important in some countries, but are generally neglected in development programmes. The expansion and intensification of smallholder dairy production is fueled by increased demand for milk with associated problems of milk handling and distribution, hygiene and environmental pollution. The major constraints to production are inter alia, choice of species, breeds and availability of animals; feed resources and improved feeding systems; improved breeding, reproduction, and animal health care; management of animal manure, and organised marketing, and market outlets. These constraints provide major opportunities and challenges for research and development to increase dairy production, efficient management of natural resources, and improved livelihoods of poor farmers. Specific areas for research are identified, as also the need of a holistic focus involving interdisciplinary research and integrated natural resource management, in a shared partnership between farmers and scientists that can demonstrate increased productivity and sustainable production systems. Suggestions for performance indicators in smallholder dairy production systems are indicated.

• 한국기후변화학회지
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• 제9권3호
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• pp.223-234
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• 2018

Estimation of future land cover based on climate change scenarios is an important factor in climate change impact assessment and adaptation policy. This study estimated future land cover considering Shared Socioeconomic Pathways (SSP) using Scenario Generators. Based on the storylines of SSP1-3, future population and estimated urban area were adopted for the transition matrix, which contains land cover change trends of each land cover class. In addition, limits of land cover change and proximity were applied as spatial data. According to the estimated land cover maps from SSP1-3 in 2030, 2050, and 2100, respectively, urban areas near a road were expanded, but agricultural areas and forests were gradually decreased. More drastic urban expansion was seen in SSP3 compared to SSP1 and SSP2. These trends are similar with previous research with regard to storyline, but the spatial results were different. Future land cover can be easily adjusted based on this approach, if econometric forecasts for each land cover class added. However, this requires determination of econometric forecasts for each land cover class.