• Title/Summary/Keyword: shape detection

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Diversity of VC and incidence of hypovirulence-associated ds-RNAs in the chestnut blight fungus Cryphonectria parasitica in Korea

  • Byeongjin Cha;Jinyoung Lim;Ju, Young-Jik;Kim, Dae-Hyuk
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.23-23
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    • 2003
  • Chestnut blight, caused by Cryphonectria parasitica, is the most destructive disease of American and European chestnut trees. A total of 672 C prasitica was isolated from blight lesion on chestnut twigs, which were collected from major chestnut plantations all over Korea in 1999. Isolation rates were over 30% in Kyunggj-, Kyongnam-, and Chonnam-do. The highest isolation rate was 37.4% and recorded in Kyongnam-do. On the other hand, Chonbuk-do had the lowest isolation rate as 13.5%. In grouping of C parasitica by colony shape and color, yellow colony with irregular margin were the most dominant colony type with a frequency of 65.2%. When the 672 isolates were inoculated on the chestnut twigs, 380 isolates (56.5%) caused lesions larger than the standard virulent isolate EP155-2, while 158 isolates (23.4%) caused smaller lesions than the standard hypovirulent isolate UEP-1. In Bavendamm test that determines phenol oxidase activity, 97.1% of all the isolates resulted the same or darker discoloration than EP155-2, and only 12.2% resulted the same or lighter discoloration than UEP-1. In the vegetative compatibility (VC) tests, total 670 isolates were divided into 121 VC groups (VCGs). Kyongnam-, Chonnam-, and Chungnam-do, the three principal chestnut plantation area, had 49, 33, and 27 VCGs, respectively. Among the VCGs, the biggest VCG, KR-VC104, was composed of 164 isolates and the second biggest VCG had 62 isolates. But, 64 of 121 VCGs consisted of sole member. More than 65.8% of KR-VC104, was isolated from the three provinces, Kyongnam-, Kangwon-, and Chungbuk-do. In KR-VC104, 62.8%, 59.1%, and 85.9% of the isolates looked like virulent in colony type, pathogenicity test, and Bavendamm test. In ds-RNA detection tests using cellulose chromatography, 77 of total 650 isolates were ds-RNA positive and detected ds-RNA segments were approximately 12kb, 3kb, 2.7kb, 2kb, and 1.8kb in size. Among the 77 isolates, 46 isolates had 12kb and 25 isolates had 12kb and 2.7kb. Other 6 Isolates had small ds-RNA segments. Kyongnam-, Chonnam-, and Chungnam-do had 43, 16, and 5 ds-RNA positive isolates, respectively. Among the 121 VCGs, only 29 VCGs had ds-RNA positive isolates.(중략)

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Construction of a DNA Profile Database for Commercial Cucumber (Cucumis sativus L.) Cultivars Using Microsatellite Marker (Microsatellite 마커를 이용한 오이 유통품종 DNA Profile Data Base 구축)

  • Kwon, Yong-Sham;Choi, Keun-Jin
    • Horticultural Science & Technology
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    • v.31 no.3
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    • pp.344-351
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    • 2013
  • Microsatellite is one of the most suitable marker for cultivar identification as it has great discrimination power for cultivars with narrow genetic variation. The polymorphism level between 358 microsatellite primer pairs and 11 commercial cucumber cultivars was investigated. Thirty-one primer pairs showed high polymorphism within cucumber cultivars with different fruit types. These markers were applied for the constructing DNA profile data base of 110 commercial cucumber cultivars through multiplex PCR and fluorescence based automatic detection system. A total of 139 polymorphic amplified fragments were obtained by using 31 microsatellite markers. The average of PIC value was 0.610 ranging from 0.253 to 0.873. One hundred and thirty nine microsatellite loci were used to calculate Jaccard's distance coefficients for UPGMA cluster analysis. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into plant shape and fruit type. Almost the cultivars were discriminated by marker genotypes. This information may be useful to compare through genetic relationship analysis between existing variety and candidate varieties in distinctive tests and protection of plant breeders' intellectual property rights through variety identification.

Alternative Tracing Method for Moving Object Using Reference Template in Real-time Image - Focusing on Parking Management System (참조 템플릿 기반 실시간 이동체 영상을 이용한 대안적 탐지 방안 - 주차관리시스템을 대상으로)

  • Joo, Yong Jin;Kang, Lee Seul;Hahm, Chang Hahk
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.32 no.5
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    • pp.495-503
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    • 2014
  • As the number of vehicles has been sharply increases, the significance of safety and effective operation issues in the parking lot is being emphasized, which takes a part of the transportation system. Recently, there have been several studies for the parking management by detecting moving object, however, recognizing numbers of fast-moving vehicles simultaneously in the picture is still a challenging problem. The parking lot in public area, or large-sized buildings has clear parking section, whereas the sensor system is configured to monitor a plurality of parking spaces. Therefore, by considering those parking lots, we suggested to develop the real-time parking availability information system by applying the real-time image processing techniques. with the help of template matching. Following the study, we wanted to provide the alternative method for parking management system through the reference template makers by recognizing movements of parked vehicles with the size and shape, regardless of direct detecting of driving movements. In addition, we evaluated the applicability and performances of the information system, presented in this study, and implemented a prototype system to simulate the parking statuses of each floor. In fat, it was possible to manage and analyze statistics about the total number of parking spaces and the number of vehicles parked through real-time video flames. We expected that the result of the study will be advanced, following the user-friendliness and cost reduction in operating parking management system and giving information by efficient analysis of parking situation.

A Study on the Fabrication and Physical Properties of $Ca_2SiO_4$:La Thermoluminescent Phosphors ($Ca_2SiO_4$: La 열형광체 제작과 물리적 특성에 관한 연구)

  • Kim, Choung-Mi;Seo, Mi-Kyong
    • Journal of the Korean Society of Radiology
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    • v.4 no.4
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    • pp.5-10
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    • 2010
  • The $Ca_2SiO_4$ phosphors doped by La with 0.1 wt%, 0.3 wt%, 0.5 wt%, and 1.0 wt% concentration were prepared by sintering at $1000^{\circ}C$ for 90 minutes in N2 atmosphere. The phosphors were ground in powdered form and were grouped in $100{\mu}m$ size, then the samples had been exposed to low energy X-ray and UV light. The TL glow curves were measured by heating the phosphors at $10^{\circ}C/s$ rate. There was no significantly meaningful correlation between the TL intensity and the doping level. The intensities of the TL peak measured from X-ray irradiated samples doped with 0.1 wt% were relatively strong. The activation energy and the frequency factor were 0.434 ~ 0.516 eV and 0.5 ~ 0.56, respectively. The intensities of the TL peak measured from UV irradiated samples doped with 0.3 wt% were relatively strong. The activation energy and frequency factor were 0.415 ~ 0.477 eV and 0.5 ~ 0.53, respectively. The TL process were found to be the 2nd order for both X-ray and UV irradiation. The TL intensity was increased linearly with the increase of the radiation dose. In summary, the $Ca_2SiO_4 phosphors developed in this study showed good TL characteristics at low energy X-ray and UV light. We believe they will be used as TLDs in near future for personal and environmental radiation detection dosimetry.

The Research of Shape Recognition Algorithm for Image Processing of Cucumber Harvest Robot (오이수확로봇의 영상처리를 위한 형상인식 알고리즘에 관한 연구)

  • Min, Byeong-Ro;Lim, Ki-Taek;Lee, Dae-Weon
    • Journal of Bio-Environment Control
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    • v.20 no.2
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    • pp.63-71
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    • 2011
  • Pattern recognition of a cucumber were conducted to detect directly the binary images by using thresholding method, which have the threshold level at the optimum intensity value. By restricting conditions of learning pattern, output patterns could be extracted from the same and similar input patterns by the algorithm. The algorithm of pattern recognition was developed to determine the position of the cucumber from a real image within working condition. The algorithm, designed and developed for this project, learned two, three or four learning pattern, and each learning pattern applied it to twenty sample patterns. The restored success rate of output pattern to sample pattern form two, three or four learning pattern was 65.0%, 45.0%, 12.5% respectively. The more number of learning pattern had, the more number of different out pattern detected when it was conversed. Detection of feature pattern of cucumber was processed by using auto scanning with real image of 30 by 30 pixel. The computing times required to execute the processing time of cucumber recognition took 0.5 to 1 second. Also, five real images tested, false pattern to the learning pattern is found that it has an elimination rate which is range from 96 to 98%. Some output patterns was recognized as a cucumber by the algorithm with the conditions. the rate of false recognition was range from 0.1 to 4.2%.

A Study on Efficiency Error in Distance Inverse Square Law using Cylinder NaI(Tl) Scintillation Detector (원통형 NaI(Tl) 신틸레이션 검출기를 이용한 거리의 역자승 법칙에서 효율 오류에 대한 연구)

  • Lee, Samyol;Yoon, Jungran;Ro, TaeIk
    • Journal of the Korean Society of Radiology
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    • v.7 no.5
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    • pp.333-338
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    • 2013
  • Generally, it's known fact that intensity of radioactivity satisfies inverse-square law. However, the law was dissatisfied with practical experiment because of limited shape of scintillation detector. Especially, in the case of near distance between the surface of detector and the radioactive source, the difference grows larger. In the present study, reason of this difference was confirmed by experiment with $2^{{\prime}{\prime}}{\times}2^{{\prime}{\prime}}{\phi}$ NaI(Tl) scintillation detector and $^{60}Co$(1.174 MeV, 1.333 MeV)and $^{137}Cs$(0.662 MeV) gamma ray sources. From the experiment, the correction coefficient was obtained with gamma ray detection efficiency and geometrical volume. In the result of the present study, the efficiency difference of the detector was corrected with the coefficient. In the present result, we obtained that the inverse-square law experiment have to consider the efficiency and geometrical value of the detector.

Wavelet-based Pitch Detector for 2.4 kbps Harmonic-CELP Coder (2.4 kbps 하모닉-CELP 코더를 위한 웨이블렛 피치 검출기)

  • 방상운;이인성;권오주
    • The Journal of the Acoustical Society of Korea
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    • v.22 no.8
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    • pp.717-726
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    • 2003
  • This paper presents the methods that design the Wavelet-based pitch detector for 2,4 kbps Harmonic-CELP Coder, and that achieve the effective waveform interpolation by decision window shape of the transition region, Waveform interpolation coder operates by encoding one pitch-period-sized segment, a prototype segment, of speech for each frame, generate the smooth waveform interpolation between the prototype segments for voiced frame, But, harmonic synthesis of the prototype waveforms between previous frame and current frame occur not only waveform errors but also discontinuity at frame boundary on that case of pitch halving or doubling, In addtion, in transition region since waveform interpolation coder synthesizes the excitation waveform by using overlap-add with triangularity window, therefore, Harmonic-CELP fail to model the instantaneous increasing speech and synthesis waveform linearly increases, First of all, in order to detect the precise pitch period, we use the hybrid 1st pitch detector, and increse the precision by using 2nd ACF-pitch detector, Next, in order to modify excitation window, we detect the onset, offset of frame by GCI, As the result, pitch doubling is removed and pitch error rate is decreased 5.4% in comparison with ACF, and is decreased 2,66% in comparison with wavelet detector, MOS test improve 0.13 at transition region.

Development of Software Correlator for KJJVC (한일공동VLBI상관기를 위한 소프트웨어 상관기의 개발)

  • Yeom, J.H.;Oh, S.J.;Roh, D.G.;Kang, Y.W.;Park, S.Y.;Lee, C.H.;Chung, H.S.
    • Journal of Astronomy and Space Sciences
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    • v.26 no.4
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    • pp.567-588
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    • 2009
  • Korea-Japan Joint VLBI Correlator (KJJVC) is being developed by collaborating KASI (Korea Astronomy and Space Science Institute), Korea, and NAOJ(National Observatory of Japan), Japan. In early 2010, KJJVC will work in normal operation. In this study, we developed the software correlator which is based on VCS (VLBI Correlation Subsystem) hardware specification as the core component of KJJVC. The main specification of software correlator is 8 Gbps, 8192 output channels, and 262,144-points FFT (Fast Fourier Transform) function same as VCS. And the functional algorithm which is same as specification of VCS and arithmetic register are adopted in this software correlator. To verify the performance of developed software correlator, the correlation experiments were carried out using the spectral line and continuum sources which were observed by VERA (VLBI Exploration of Radio Astrometry), NAOJ. And the experimental results were compared to the output of Mitaka FX correlator by referring spectrum shape, phase rate, and fringe detection and so on. Through the experimental results, we confirmed that the correlation results of software correlator are the same as Mitaka FX correlator and verified the effectiveness of it. In future, we expect that the developed software correlator will be the possible software correlator of KVN (Korean VLBI Network) with KJJVC by introducing the correlation post-processing and modifying the user interface as like GUI (Graphic User Interface).

Endo- and Epi-cardial Boundary Detection of the Left Ventricle Using Intensity Distribution and Adaptive Gradient Profile in Cardiac CT Images (심장 CT 영상에서 밝기값 분포와 적응적 기울기 프로파일을 이용한 좌심실 내외벽 경계 검출)

  • Lee, Min-Jin;Hong, Helen
    • Journal of KIISE:Software and Applications
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    • v.37 no.4
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    • pp.273-281
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    • 2010
  • In this paper, we propose an automatic segmentation method of the endo- and epicardial boundary by using ray-casting profile based on intensity distribution and gradient information in CT images. First, endo-cardial boundary points are detected by using adaptive thresholding and seeded region growing. To include papillary muscles inside the boundary, the endo-cardial boundary points are refined by using ray-casting based profile. Second, epi-cardial boundary points which have both a myocardial intensity value and a maximum gradient are detected by using ray-casting based adaptive gradient profile. Finally, to preserve an elliptical or circular shape, the endo- and epi-cardial boundary points are refined by using elliptical interpolation and B-spline curve fitting. Then, curvature-based contour fitting is performed to overcome problems associated with heterogeneity of the myocardium intensity and lack of clear delineation between myocardium and adjacent anatomic structures. To evaluate our method, we performed visual inspection, accuracy and processing time. For accuracy evaluation, average distance difference and overalpping region ratio between automatic segmentation and manual segmentation are calculated. Experimental results show that the average distnace difference was $0.56{\pm}0.24mm$. The overlapping region ratio was $82{\pm}4.2%$ on average. In all experimental datasets, the whole process of our method was finished within 1 second.

Evaluation of Automatic Image Segmentation for 3D Volume Measurement of Liver and Spleen Based on 3D Region-growing Algorithm using Animal Phantom (간과 비장의 체적을 구하기 위한 3차원 영역 확장 기반 자동 영상 분할 알고리즘의 동물팬텀을 이용한 성능검증)

  • Kim, Jin-Sung;Cho, June-Sik;Shin, Kyung-Sook;Kim, Jin-Hwan;Jeon, Ho-Sang;Cho, Gyu-Seong
    • Progress in Medical Physics
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    • v.19 no.3
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    • pp.178-185
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    • 2008
  • Living donor liver transplantation is increasingly performed as an alternative to cadaveric transplantation. Preoperative screening of the donor candidates is very important. The quality, size, and vascular and biliary anatomy of the liver are best assessed with magnetic resonance (MR) imaging or computed tomography (CT). In particular, the volume of the potential graft must be measured to ensure sufficient liver function after surgery. Preoperative liver segmentation has proved useful for measuring the graft volume before living donor liver transplantations in previous studies. In these studies, the liver segments were manually delineated on each image section. The delineated areas were multiplied by the section thickness to obtain volumes and summed to obtain the total volume of the liver segments. This process is tedious and time consuming. To compensate for this problem, automatic segmentation techniques have been proposed with multiplanar CT images. These methods involve the use of sequences of thresholding, morphologic operations (ie, mathematic operations, such as image dilation, erosion, opening, and closing, that are based on shape), and 3D region growing methods. These techniques are complex but require a few computation times. We made a phantom for volume measurement with pig and evaluated actual volume of spleen and liver of phantom. The results represent that our semiautomatic volume measurement algorithm shows a good accuracy and repeatability with actual volume of phantom and possibility for clinical use to assist physician as a measuring tool.

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