• Title/Summary/Keyword: shaker like

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A Study on Treatment of Soils Contaminated by Diesel and Kerosene Using Hydrogen Peroxide Catalyzed by Naturally Occurring Iron Minerals (디젤과 등유로 오염된 토양의 철광석으로 촉매화된 과수를 이용한 처리에 관한 연구)

  • Choi, Jin-Ho;Kim, Sang-Dae;Moon, Sei-Ki;Kong, Sung-Ho
    • Applied Chemistry for Engineering
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    • v.10 no.1
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    • pp.24-29
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    • 1999
  • Naturally-occurring iron minerals, goethite, magnetite, and hydrogen peroxide were used to catalyze and initiate Fenton-like oxidation of silica sand contaminated with mixture of diesel and kerosene in batch system. Optimal reaction conditions were investigated by varying pH(3, 7), $H_2O_2$ concentration(0%, 1%, 7%, 15%, 35%), initial contaminant concentration(0.2, 0.5, 1.0 g-mixture of diesel and kerosene/ kg-soil), and iron mineral contents(1, 5, and 10 wt % magnetite or goethite). Contaminant degradations in silica sand-iron mineral-$H_2O_2$ systems were identified by determining total petroleum hydrocarbon(TPH) concentration. The optimal pH of the system was 3. The system which iron minerals were the only iron source was more efficient than the system with $FeSO_4$ solution due to lower $H_2O_2$ consumption. In case of initial contaminant concentration of 1g-contaminant/kg-soil with 5 wt % magnetite, addition of 0%, 1%, 7%, 15%, and 35% of $H_2O_2$ showed 0%, 24.5%, 44%, 52%, and 70% of TPH reduction in 8 days, respectively. When the mineral contents were varied 0, 1, 5, and 10wt%, removal of contaminants were 0%, 33.5%, 50%, and 60% for magnetite and 0%, 29%, 41%, and 53% for goethite, respectively. Reaction of magnetite system showed higher degradation than that of goethite system due to dissolution of iron and mixed presence of iron(II) and iron(III); however, dissolved iron precipitated on the surface of iron mineral and seemed to cause reducing electron transfer activity on the surface and quenching $H_2O_2$. The system using goethite has better treatment efficiency due to less $H_2O_2$ consumption. When cach system was mixed by shaker, removal of contaminants increased by 41% for magnetite and 30% for goethite. Results of this study showed catalyzed $H_2O_2$ system made in-situ treatment of soil contaminated with petroleum possible without addition of iron source since natural soils generally contain iron minerals such as magnetite and goethite.

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Characteristics of Monascus Natural Pigments Produced by Monascus sp. MK2-2 (Monascus sp. MK2-2가 생산하는 홍국천연색소의 특성)

  • Jeon, Chun-Pyo;Kim, Chang-Suk;Lee, Jung-Bok;Shin, Ji-Won;Choi, Sung-Yeon;Choi, Chung-Sig;Lee, Oh-Seuk;Kwon, Gi-Seok
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.137-142
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    • 2007
  • For the production of natural pigments with microbe, the strains which produced monascus pigment were isolated, and then culture condition and extraction condition were investigated. These results are summarized as follows; The strain which ran produce monascus natural pigment was isolated from natural microbial sources and we made mutant of this strain with UV($235_{nm}$, 30 second) irradiation. The mutant was identified as Monascus sp. MK2-2. The optimal culture conditions were investigated optimal medium containing 0.3% rice powder, 0.2% yeast extract, 0.3% $NH_4H_2PO_4$ and $30^{\circ}C$ in a rotary shaker (120 rpm) for 5 days (initial pH 5.0), while the pigment production was determined at 24 hr intervals. The effective carbon sources were wheat flour > rice powder > fructose, and effective nitrogen sources were sodium nitrate > $KNO_3$ for production of the monascus natural pigment. The pigment capacity is good from 17 to 22 in C/N ratio. The production amount of monascus natural pigment was 0.38 g per 1 kg of rice. Also, extract of red yeast rice had anti-thrombosis activity like a degree of aspirin.

Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture

  • Dai, Yu-Jie;Li, Jing;Wei, Shu-Mei;Chen, Nan;Xiao, Yu-Peng;Tan, Zhi-Lei;Jia, Shi-Ru;Yuan, Nan-Nan;Tan, Ning;Song, Yi-Jie
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.534-538
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    • 2013
  • The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under $27{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light intensity and $25^{\circ}C$ on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.