• Title/Summary/Keyword: sewerage system

Search Result 109, Processing Time 0.029 seconds

Development of Molecular Diagnostic System with High Sensitivity for the Detection of Human Sapovirus from Water Environments

  • Lee, Siwon;Bae, Kyung Seon;Lee, Jin-Young;Joo, Youn-Lee;Kim, Ji-Hae;You, Kyung-A
    • Biomedical Science Letters
    • /
    • v.27 no.1
    • /
    • pp.35-43
    • /
    • 2021

  • Human Sapovirus (HuSaV) is one of the major causes of acute gastroenteritis in humans, and it is used as a molecular diagnostic technique based on polymerase chain reaction (PCR) from humans, food, shellfish, and aquatic environments. In this study, the HuSaV diagnosis technique was used in an aquatic environment where a number of PCR inhibitors are included and pathogens, such as viruses, are estimated to exist at low concentration levels. HuSaV-specific primers are improved to detect 38 strains registered in the National Center for Biotechnology Information (NCBI). The established optimal condition and the composition, including the RT-nested PCR primers and SL® Non-specific reaction inhibitor, were found to have 100 times higher sensitivity based on HuSaV plasmid than the previously reported methods (100 ag based on HuSaV plasmid 1 ng/μL). Through an artificial infection test, the developed method was able to detect at least 1 fg/μL of HuSaV plasmid contaminated with total nucleic acid extracted from groundwater. In addition, RT-nested PCR primer sets for HuSaV detection can react, and a positive control is developed to verify false positives. This study is expected to be used as a HuSaV monitoring method in the future and applied to the safety response to HuSaV from water environments.

  • https://doi.org/10.15616/BSL.2021.27.1.35 인용 PDF KSCI

Appropriate Sewerage Systems for Korea (우리나라 적합 하수도시설 및 관리방안)

  • 이상은
    • Journal of environmental and Sanitary engineering
    • /
    • v.7 no.2
    • /
    • pp.37-52
    • /
    • 1992

  • Since the first sewage treatment plant was constructed in 1976, the sewerage systems of Korea have been rapidly expanded. As of the end of 1991, 22 sewage treatment plants with total capacity of 5.4 million tons/day are in operation which is equivalent of 3395 total daily sewage generation. Total extension of sewer 39.534 km in 1990 which is 55% of the target extension for the year 2001. However, the most sewage treatment plants employ activated sludge process which may not be suitable for medium and/or small scale plants. The poor existing sewer systems do not effectively collect and transport sewage to adversely affect the function of sewage treatment plant. To select the appropriate treatment system, the cities are classified into 3 categories such as large and medium size inland cities, small size cities and coastal cities. Considering the criteria suggested during this study, appropriate treatment processes were selected for each category. Conventional activated sludge process and step aeration process were found to be the most appropriate for big inland cities while biological nutrient removal processes should be considered for the cities discharge the effluent to lakes or reservoirs. RBC or Oxidation Ditch process might be appropriate for the medium size cities while several processes which do not require skilled operation and maintenance were suggested for the small cities. Ocean discharge after primary treatment can be considered for some east coast cities, Appropriate methodology to rehabilitate the existing sewers and strategy to convert combined sewer system to separate sewer system were proposed. This paper also include the appropriate management system for industrial wastewater, sludge and nightsoil.

  • PDF

Effect of municipal sludge from drainage facility on foul smell (도시내 배수설비 슬러지가 악취에 미치는 영향 연구)

  • Yu, Dae-Hyun;Park, Jihoon;Lee, Yong-Hoon;Lee, Jang-Hown;Kang, Seon-Hong
    • Journal of Korean Society of Water and Wastewater
    • /
    • v.28 no.3
    • /
    • pp.315-324
    • /
    • 2014

  • The residents' odor complaint is steadily increasing by odor causing from sewage system. A current drainage facilities and septic tank are included in the individual sewerage according to Sewerage Act. However, definitions and legal standards of drainage tank are insufficient. In addition, specifications and maintenance standards related to structure and size are not established. In this research, effect of drainage tank in individual sewage facilities on the odor occurrence was studied and the concentrations of $H_2S$ and composite odor were measured. From the measurements, the concentration of $H_2S$ and composite odor were 1 ppm ~ 5 ppm and 1.04 OU ~ 4.05 OU, respectively, before pump operation. Also, the concentration of $H_2S$ and composite odor were 5 ppm ~ 33 ppm and 5.10 OU ~ 35.04 OU, respectively, after pump operation. The concentration of SS in the effluent from drainage tank was ranged from 840 mg/L to 1,980 mg/L. These high concentration of SS seemed to be the major source of foul smell when high concentrated suspended solids in the public sewerage system were decomposed and then emitted. In this research, correlation coefficient ($R^2$) between $H_2S$ and composite odor before and after pumping were 0.925 and 0.918, respectively.

  • https://doi.org/10.11001/jksww.2014.28.3.315 인용 PDF KSCI