• Journal of Species Research
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• v.9 no.4
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• pp.325-338
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• 2020

The animal gut is filled with highly diverse microbes associated with host metabolism, physiology, and pathology. However, numerous animal gut microbes have not been cultured or reported. We isolated various bacterial species using culture-dependent approaches during a comprehensive investigation of endangered endemic vertebrate species in the Republic of Korea. A total of 18 unrecorded bacterial species were isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus). Based on a phylogenetic analysis of 16S rRNA gene sequences, we discovered species belonging to the phyla Actinobacteria (eight species), Firmicutes (seven species), Proteobacteria (two species), and Bacteroidetes (one species). Based on their high 16S rRNA gene sequence similarities (>98.7%) and formation of monophyletic clades with type species, each species was classified into an independent and predefined bacterial species. Gram-stain reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and NIBR IDs for each species are described in the species description section.

• Journal of Species Research
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• v.6 no.2
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• pp.119-128
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• 2017

Within a comprehensive, widescale investigation of indigenous prokaryotic species in Korea, 29 bacterial strains in the phylum Bacteroidetes were isolated from diverse environmental habitats that included soil, plant roots, natural caves, tidal flats, freshwater from lakes, and seawater. Based on their high 16S rRNA gene sequence similarities (>99.1%) and the formation of robust phylogenetic clades with the closest type species, each strain likely belonged to an independent and predefined bacterial species. There are no publications or official reports of the isolation of these 29 species in Korea. Our study provides strong evidence that seven species in three genera in the order Cytophagales, 15 species in 13 genera in the order Flavobacteriales and seven species in five genera in the order Sphingobacteriales, all within the phylum Bacteriodetes, are new reports of bacterial species in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are described in the species description section.

• Animal Systematics, Evolution and Diversity
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• v.8 no.1
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• pp.141-160
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• 1992

The systematic study on the bryozoans from the South SEa in Korea was done with the materials collected at 49 localities from 1965 to 1991. As a result, 57 species in two suborders of cheilostomaous bryozoans were identified, which seven species, Electra tenella, Labioporella bursaria, Dendrobeania longispinosa, Sinupetraliella gigantea, Cryptosula pallasiana, Hippopodina feegeensis and Durystomella bilabiata were new to Korean fauna. The unrecorded species and the species which need remarking were described with plates.

• Journal of Species Research
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• v.7 no.1
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• pp.13-23
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• 2018

In 2016, as a part of the research program 'Survey of Korean Indigenous Species', diverse environmental samples were collected from various sources of freshwater, seawater, soil, wetland, reclaimed land, sand, pine forest, plant root, ginseng field, solar saltern, and caves. Thousands of bacterial strains were isolated from the diverse samples and identified based on 16S rRNA gene sequence analyses. The present study, as a phylogenetic subset of the primary research program, reports 24 unrecorded bacterial species in Korea that belong to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria. Based on the high 16S rRNA gene sequence similarities (>98.8%) and formation of a robust phylogenetic clade with the closest type species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 24 bacterial species have been described in Korea; therefore, 10 species of nine genera in the order Rhizobiales and 14 species of seven genera in the order Sphingomonadales are described for unreported alphaproteobacterial species in Korea. Gram reaction, colony and cell morphology, biochemical properties, and isolation sources are also provided in the species description section.

• Journal of Species Research
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• v.8 no.2
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• pp.176-190
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• 2019

A total of 38 bacterial strains within the classes Bacilli and Deinococci were isolated from various sources in Korea. Samples were collected from animal intestine, urine, soil, tidal flat mud, and kimchi. In the sequence comparison and phylogenetic analysis of 16S rRNA sequences, the 38 isolates were assigned to the classes Bacilli and Deinococci with sequence similarities more than 98.7%. Twenty-four strains and 13 strains were classified the order Bacillales and Lactobacillales in the class Bacilli, respectively. In the order Bacillales, there were nine species in the genus Bacillus, seven species in the genus Paenibacillus, and the remaining eight species in the genera Domibacillus, Halobacillus, Virgibacillus, Lysinibacillus, Paenisporosarcina, Planococcus, Savagea, and Staphylococcus. In the order Lactobacillales, there were four species in the genus Lactobacillus, three species in the genus Leuconostoc, three species in the genus Lactococcus, and the remaining three species in the genera Aerococcus, Enterococcus, and Streptococcus. One species was related to the genus Deinococcus of the order Deinococcales. Most of the isolated strains were Gram-stain-positive, but some were Gram-stain-variable or Gram-stain-negative. Cells were rod or cocci-shaped. Based on the results of 16S rRNA analysis, we report 38 strains as previously unrecorded species to Korea, and the basic characteristics of strains are described herein.

• Korean Journal of Environmental Biology
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• v.39 no.2
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• pp.236-244
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• 2021

Unarmored dinoflagellates, in the family Kareniaceae, include harmful or toxic bloom-forming species, which are associated with massive fish kills and mortalities of marine organisms worldwide. The occurrence and distribution of the toxigenic species in the family Kareniaceae were investigated in the brackish and coastal waters of Korea between July 2018 and October 2020. During the survey, we collected seven newly recorded species; Karenia papilionacea, Karlodinium digitatum, Karl. veneficum, Karl. zhouanum, Takayama acrotrocha, T. helix, and T. tasmanica. A total of fifteen strains of the seven taxa were successfully established as clonal cultures and examined using LM, SEM, and molecular phylogeny inferred from LSU rDNA sequences. Herein, we present the taxonomic information, morphological features, and molecular phylogenetic positions of the unrecorded dinoflagellate species collected from Korean coastal waters.

• Journal of Species Research
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• v.6 no.2
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• pp.129-140
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• 2017

During a comprehensive investigation of indigenous prokaryotic species in Korea, a total 31 bacterial strains assigned to the class Alphaproteobacteria were isolated from diverse environmental habitats including freshwater, seawater, brackish water, ginseng soil, plant roots, natural caves, and tidal flats. Based on their high 16S rRNA gene sequence similarities(>99.1%) and formation of robust phylogenetic clades with the closest type species, each strain was assigned to an independent and predefined bacterial species. Because there were no published or official reports regarding the isolation of these 31 species in Korea, this study identified three species in two genera in the order Caulobacterales, 12 species in 10 genera in the order Rhodobacterales, three species in two genera in the order Rhizobiales, two species in two genera in the order Rhodospirillales and 11 species in seven genera, all in the order Sphingomonadaceae within the Alphaproteobacteria are reported as new alphaproteobacterial species in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are described in the species description section.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.491-504
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• 2023

To explore fungal diversity in orchard soil where fire-blighted apple trees are buried, we collected soil samples from apple orchards in Chungju, Korea. Fungal isolates were obtained from DG18 agar and identified at the species level based on morphological features and phylogenetic analyses. The colony characteristics and microstructures were examined using a light microscope and a scanning electron microscope after culturing on potato dextrose agar (PDA), malt extract agar (MEA), Czapek yeast agar (CYA), and oatmeal agar (OA) The PCR-amplified products of the ITS1-5.8S-ITS2 region and 28S large subunit of the nuclear ribosomal RNA gene, as well as partial sequences of the β-tubulin, calmodulin, and translation elongation factor 1-α genes were sequenced and analyzed phylogenetically. Seven previously unknown fungal species were explored in Korea. All samples, including Aspergillus aureolatus, Botryotrichum atrogriseum, Dactylonectria novozelandica, Fusarium denticulatum, Paecilomyces tabacinus, Sarcopodium tibetense and Talaromyces stollii, had ascomycetes. Herein, we report their descriptions and features.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.5-14
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• 1997

The fish fauna was investigated on summer (from July 28 to Aug. 2) and winter (December, 1994) at the Munsom in Cheju-do. As a result of this study, the fishes identified in these areas were classified into 79 species involved in 35 families. And seven species unrecorded in Korea were found, i.e. Halicampus boothae, Cirrhitichthys aprinus, Stegastus altus, Parupeneus sp., Pomacentrus sp., Pomacentrus sp., and Pomacentridae sp. In this region large number species of family Pomacentridae (12 species), family Labridae (9 species), Scorpaeinidae(6 species) and Blennidae (5 species) were observed in summer.

• Journal of Species Research
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• v.11 no.1
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• pp.67-70
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• 2022

One of the small genera of the tribe Atrophini (subfamily Banchinae), the genus Amphirhachis, has been reported seven species in the world. Among them, four species (Amphirhachis fasciata, A. nigripalpis, A. rubriventris and A. tertia) from Oriental region, and four species(Amphirhachis fujiei, A. miyabi, A. nigra and A. tertia) from Eastern Palaearctic region. Unfortunately, there are no records in South Korea yet. This genus is easily distinguished from other genera of Atrophini following: clypeus strongly convex near side; posterior transverse carina of propodeum represented by a weak or faint vertical ridge at apex on each side; ovipositor sheath shorter than metasoma. In this study, the genus Amphirhachis is recorded for the first time with a newly recorded species (Amphirhachis tertia) from South Korea. New data on the taxonomy and distribution of Amphirhachis tertia are given. We also provide diagnosis and digital images of a newly recorded genus and species.