• Journal of Microbiology and Biotechnology
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• 제10권3호
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• pp.367-374
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• 2000

The composition of dissolved gases and nutrients in a liquid medium were determined for establishment of the optimum conditions for in vitro culture of Helicobacter pylori. A microaerobic condition facored by the organism was prepared by adjusting the partial pressure of the gas, agitation speed, and viscosity of the medium. The gaseous concentrations were controlled by utilizing CampyPak Plus that reduced oxygen while augmenting carbon dioxide. Agitation of the broth facilitated the oxygen transfer to the cells, yet inhibited the growth at high rates. An increase of viscosity in the medium repressed the culture although this variable was relatively insignificant. The chemical constituents of the liquid broth were examined to establish an economic model for H. pylori cultivation. The microbe required a neutral pH for optimum growth, and yet was also able to proliferate in an acidic condition, presumably by releasing the acidity-modulating enzyme, urease. Cyclodextrin and casamino acid were investigated as growth enhancers in place of serum, while yeast extract unexpectedly inhibited the cells. A low concentration of glucose, the unique carbon source for the organism, increased the cell density, yet high concentrations resulted in an adverse effect. Under optimally dissolved gas conditions, the cell concentration in brucella broth supplemented with serum substitutes and glucose reached $1.6{\times}10^8$ viable cells/ml which was approximately 50% higher than that obtained in the liquid medium added with only cyclodextrin or serum.

• Journal of Periodontal and Implant Science
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• 제31권3호
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• pp.513-529
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• 2001

For reconstruction of the bony defect, various artificial substitutes were developed. Among them, there has been a study of calcium phosphate coated bone substitutes for increasing attachment of osteoblasts in vivo. The purpose of this study was to evaluate the effects of serum and platelet-rich plasma (PRP) on calcium phosphate coated culture plate for the initial attachment, proliferation and activity of osteoblasts. After sampling the blood from white rats and concentrating by centrifugation, the amount of attachment of PDGF-BB and $TGF-{\beta}$ on the calcium phosphate coated culture plate was measured. Cultured HOS and ROS 17/2.8 cell was measured on attachment level and proliferation rate of osteoblasts. Alkaline phosphatase activity of HOS and ROS 17/2.8 cell was measured for studying on the activating rate of osteoblast. 1. Counting the amount of platelets of seperated plasma and PRP, the average number of platelets was 177,003 $cell/{\mu}l$ in plasma, and 1,656,062 $cell/{\mu}l$ in PRP, which was about 9 times as high as in plasma. 2. Amount of PDGF-BB deposited at calcium phosphate coated plate had increased by the total amount of plasma and PRP on the culture plate, whereas $TGF-{\beta}$had been deposited on the plate only when treated by $50{\mu}{\ell}$ of PRP(p<0.01). 3. After plating serum and PRP for 3 hours, we attached with HOS and ROS17/2.8 cell for 1 hour and 4 hours. There were no significant difference of the attachment between serum and control group, whereas there were significantly difference of the attachment between depositioning of PRP and control group. 4. After attaching plasma and PRP for 3 hours, cell number has much increased when HOS and ROS17/2.8 cell had been cultured for 48 hours(p<0.05). 5. After attaching plasma and PRP for 3 hours, concentration of alkaline-phosphatase has increased when HOS and ROS17/2.8 cell had been cultured for 48 hours(p<0.01). These results suggested that PRP affected on initial cell attachment rather than proliferation and activation of osteoblasts at calcium phosphate coated plate.

• Animal Bioscience
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• 제34권2호
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• pp.205-212
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• 2021

Objective: The purpose of present study was to investigate the effects of condensed molasses fermentation solubles (CMS) on lactation performance, rumen fermentation, nutrient digestibility, and serum parameters of dairy cows. Methods: A total of 75 healthy Holstein cows with the same parity (milk production = 35±2.5 kg, body weight = 570±28 kg) were randomly selected and divided into 5 groups. One group served as control group (CON; no CMS), whereas the other 4 groups were CMS1 (accounted for 1% of the diet), CMS2 (2%), CMS3 (3%), and CMS4 (4%). All cows were fed regularly three times each day at 0800, 1600, and 2400 h. Cows received diet and water ad libitum. The experiment lasted for 60 days. Results: Results showed that the dry matter intake, milk yield, and protein of CMS2 were maximum and higher (p<0.05) than CMS4. The ruminal pH was observed less than 6 in CMS3 and CMS4 groups. No noticeable difference of microbial protein was found between CON and CMS2 groups, while the microbial protein in these groups was higher (p<0.05) than CMS3 and CMS4 groups. The apparent digestibility of dry matter, organic matter, and crude protein in CMS2 group was higher (p<0.05) than CMS3 and CMS4 groups. Compared to CMS3 and CMS4 groups, the CMS2 group increased (p<0.05) the serum concentrations of immunoglobulin G and immunoglobulin M on d 60. Conclusion: Therefore, it is practicable that CMS substitutes for a part of concentrates in lactating cows' diets, but higher addition of CMS (more than 3% of the diet) could decrease production performance of dairy cows as seen in the present study.

• 한국식품영양과학회지
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• 제34권5호
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• pp.626-631
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• 2005

새송이버섯이 고지방식이를 섭취한 흰쥐의 지질대사에 미치는 영향을 조사하고자, 성숙한(생후 21주령) 숫쥐에 표준식 이에 $20\%$ 돈지를 첨가한 식이를 급여한 대조군, 대조식이에 새송이 버섯 분말을 $3\%$ 및 $5\%$씩 첨가한 식이를 급여한 군($3\%$ 및 $5\%$ 새송이버섯군) 등 3군으로 나누어 10주간 사육한 결과는 다음과 같다. 실험동물의 체중증가량 및 식이효율은 대조군에 비하여 $3\%$ 및 $5\%$ 새송이버섯군이 유의하게 감소되었다. 간과 신장 및 부고환지방의 무게는 대조군과 새송이버섯군들이 비슷하였다. 간의 총 지질 및 중성지질 농도는 대조군에 비하여 새송이버섯군들이 유의하게 감소 되었다. 혈청의 총 콜레스테롤과 LDL-콜레스테롤 농도 및 동맥경화지수는 대조군에 비해 새송이 버섯군들이 유의하게 감소되었다. 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율은 대조군에 비해 새송이버섯군들이 유의하게 증가되었다. 변의 g당 총 지질배설량은 새송이버섯군이 대조군보다 유의하게 증가된 것으로 나타났다. 이상의 결과로 보아 고지방식이에 새송이버섯 분말 첨가 급여시 흰쥐 간의 중성지질 농도, 혈청의 총 콜레스테롤과 LDL-콜레스테롤 농도 및 동맥경화지수를 낮추고, 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율을 증가시키는 것으로 나타났다.

• 한국수정란이식학회지
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• 제14권2호
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• pp.113-119
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• 1999

This study was performed to improve the development of the in vitro fertilized bovine embryos by the condition of in vitro maturation. COCs were matured in TCM 199 supplemented with 0.1% PVA, 10ng/ml EGF, Hormones (5$\mu\textrm{g}$/ml FSH, 10 IU hCG, 1 $\mu\textrm{g}$/ml estradiol 17-$\beta$) or granulsa cell+Hormones atmosphere 39$^{\circ}C$, 5% CO2, 95% air for 24hrs. Matured oocytes were fertilized with frozen-thawed semen capacitated with 5mM caffein in BO medium for 20 hrs. IVF embryos were cultured in TCM 199 containing with hormones(same as matured medium), 10% FBS and co-culture with bovine oviduct epitherial cells. Maturation rates of COCs were showed 73.8%, 78.5%, 83.2% and 87.6% respectively, and were significant differences between PVA, EGF, and Hormones, GC+Hormones(p<0.05). The cleavage rates of IVF embryos were revealed 72.5%, 78.4%, 82.3% and 84.2% and showed same tendency as maturation rates(p<0.05). The blastocysts matured by above maturation condition and cultured for 7~10 days after fertilization had 34.4, 43.6, 52.3 and 59.3 cells had no differences among the treatments. These results suggest that high molecules as a substitutes of serum and growth factor may induce nuclear resumption of COCs but we need more study to produce transferable IVF blastocysts by use of that agents.

• 한국가금학회지
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• 제36권3호
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• pp.215-222
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• 2009

항생제 대체제 개발을 위한 용규와 감초 건조분말 복합제를 육계사료에 첨가 급여 하여 생산성과 혈액성상에 미치는 영향을 조사하기 위하여 수행하였다. 7일령 이후 육계 숫병아리(Ross)를 3단 케이지에 5처리 3반복, 반복당 10수씩, 총 150수를 공시하여 5주 동안 실험을 실시하였다. 시험 사료는 무첨가구, 항생제(avilamycin) 첨가구, 항생제 대체 용규 감초 건조 분말 0.5%(0.25+0.25), 1.0%(0.5+0.5%) 및 2.0%(1.0+1.0) 첨가구로 구분하여 사양 시험을 실시하였다. 증체량, 사료 섭취량 및 사료 요구율은 처리구간에 차이가 없었다. 도체중은 4주령에서 용규 감초 1.0% 첨가구에서 높았으며(P<0.05), 6주령시에는 용규 감초 1.0% 첨가구에서 약간 높았다. 도체율은 4주령과 6주령에서 처리구간에 비슷하게 측정되었다. 간장 중량은 4주령과 6주령에서 용규 감초 0.5% 첨가구에서 무거웠다(P<0.05). 비장중량은 4주령과 6주령에서 처리간 차이가 없었다. 4주령과 6주령시 혈중 protein, albumin, glucose, total bilirubin 농도와 GOT 및 GPT, TG, HDL-cholesterol, LDL-cholesterol, total cholesterol 농도에서 처리간에 차이가 없었다. 용규 감초 복합 첨가급여는 육계의 생산성 및 혈액성상에서 처리간의 차이가 없었음은 항생제 대체 물질로서의 사용가능이 있는 것으로 사료된다.