• Korean Journal of Veterinary Research
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• v.35 no.4
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• pp.699-712
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• 1995

The present study, to evaluate the effect of vitamin E on the oxidative stress in STZ-treated rat and BB rat, was investigated the biochemical enzyme activity in the serum, and malondialdehyde and carbonyl group in the RBC membrane, liver and microsomal fraction after vitamin E and/ or insulin treatment. Results obtained through the experiments were summarized as follows; 1. Effect of vitamin E and/or insulin treatment in STZ-treated rat 1) Lipid peroxidation level in RBC membrane, liver and microsomal fraction was significantly decreased in vi. tamin E and/or insulin treatment group, and especially more significantly decreased in vitamin E with insulin treated group. 2) Protein oxidation level in RBC membrane, liver and microsomal fraction was significantly decreased in vitamin E and/or insulin treatment group. And it was especially more significantly decreased in RBC membrane and liver of vitamin E with insulin treated group. 3) In the enzyme activity in the serum, the activity of AST and ALT was not altered in all experimental group. The increased ALP activity in STZ-treated group was significantly decreased in insulin treated group and vitamin E with insulin treated group. 4) Decreased level of albumin and creatinine after STZ treatment was significantly increased in vitamin E and/or insulin treated group. 5) Level of glucose, cholesterol and triacylglycerol in serum: Glucose level was not significantly different in vitamin E treated group compared to STZ control group. But it was significantly different in the insulin treated group and vitamin E with insulin treated group compared to STZ control group. The cholesterol content in the serum was significantly increased in STZ control group compared to normal control group. And except low dose vitamin E treatment group, it was significantly decreased in vitamin E and/or insulin treated group compared to STZ control group. The triacylglycerol content in the serum was significantly decreased in STZ control group and increased in high dose vitamin E treated group and vitamin E with insulin treated group. But it was not significantly different in low dose vitamin E treated group and insulin treated group compared to STZ control group. 2. Effect of vitamin E and/or insulin treatment in BB rat 1) Lipid peroxidation level in liver was decreased by vitamin E with insulin treatment compared to insulin treatment. But it was not different in microsomal fractions. 2) Protein oxidation level in liver and microsomal fraction was decreased by vitamin E with insulin treatment compared to insulin treatment only in microsomal fractions. These results suggest that the combination treatment of vitamin E and insulin could prevent the oxidative change of lipid and protein of the RBC membrane, liver and microsomal fraction in STZ-treated rats and BB rats.

• Journal of Nutrition and Health
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• v.32 no.1
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• pp.10-16
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• 1999

This study was conducted to figure out the differences of the BMI, blood lipids, blood pressure and nutrient intakes by serum insulin concentration among adults in Korea. Ninety-three subjects(male : n=37, female : n=56) participated in this study. The subjects were grouped by serum insulin concentrations into two groups-hyperinsulinemia group(n=17) and normoinsulinemia group(n=76). Anthropometric measurements, blood lipid profiles, blood pressure, oral glucose tolerance test, and daily nutrient intakes were analyzed. And serum glucose and insulin secretion pattern as shown through and oral glucose tolerance test were performed. BMI (p<0.05) and WHR(p<0.01) were significantly higher in hyperinsulinemic group than in normoinsulinemia group. There was no differences in fasting blood glucose level between groups, but total glucose area (p<0.001) and insulin glucose resistance(p<0.001) were significantly higher in hyperinsulinemia group than in normoinsulinemia higher in hyperinsulinemia group, but HDL-cholesterol(p<0.05), and systolic blood pressure(p<0.05) were significantly higher in hyperinsulinemia group, but HDL-cholesterol(p<0.001) was significantly lower in hyperinsulinemia group. They showed significant differences in energy, carbohydrate, potassium, riboflavin, niacin and dietary fiber intakes(p<0.05), the intake of those nutrients were low in normoinsulinemia group. Further investigation is necessary to determine the effects of amounts and types of carbohydrate and dietary fiber on serum insulin concentrations.

• Clinical and Experimental Reproductive Medicine
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• v.48 no.4
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• pp.374-379
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• 2021

Objective: We aimed to evaluate associations between the ratio of serum estrone (E1) to estradiol (E2) and parameters related to serum glucose metabolism and insulin resistance in women with polycystic ovary syndrome (PCOS). Methods: In total, 133 women between the ages of 18 and 35 diagnosed with PCOS were enrolled in this study. All participants with PCOS underwent blood tests to determine hormonal and biochemical metabolic parameters and a standard 2-hour 75-g oral glucose tolerance test. They were divided into two groups according to the serum E1-to-E2 ratio: group 1 (E1/E2 ratio <2.0) and group 2 (E1/E2 ratio ≥2.0). Results: In the comparative analysis, the waist-to-hip ratio (WHR) was the only clinical variable that was significantly different between the two groups. Patients with a higher E1/E2 ratio showed higher fasting insulin levels, homeostasis model for insulin resistance, and postprandial glucose level at 2 hours (PPG2). In a correlation analysis, only PPG2 was significantly related to the serum E1/E2 ratio. However, after controlling for the confounding effects of body mass index (BMI) and WHR, fasting glucose was also significantly correlated with the serum E1/E2 ratio. Conclusion: Women with PCOS with a higher serum E1/E2 ratio were found to be more likely to show higher fasting insulin and postprandial glucose levels. Significant correlations were found between the serum E1/E2 ratio and both fasting and postprandial serum glucose levels after adjusting for BMI and WHR in women with PCOS.

• Journal of Embryo Transfer
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• v.23 no.3
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• pp.189-195
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• 2008

The objective of this study is to investigate the changes in concentrations of leptin and insulin in serum of Korean cattle (Hanwoo) with reproductive disorders and to examine the relationship among leptin, insulin, and body condition score (BCS). The concentration of leptin in serum of pregnant Hanwoo showed insignificant difference from that in serum of Hanwoo with reproductive disorder, such as repeat breeding, follicular cyst, corpus luteum cyst, ovarian atrophy, and feeble estrus (p>0.05). However, the concentrations of leptin and insulin in serum were changed with different BCS value. In emaciated Hanwoo (BCS $2.0\sim2.9$), they were significantly decreased compared to BCS $3.0\sim3.4$ (p<0.05). The leptin showed different genotypes with different BCS value. In BCS $2.0\sim2.9$, C/T genotype was expressed (83.3%) more than C/C (16.7%) or T/T (0%) genotype, whereas C/C genotype was expressed (62.5%) more than C/T (25.0%) or T/T (12.5%) genotype in BCS $3.5\sim4.0$. The insulin concentration in follicular fluid obtained from ovary with follicular cyst which has follicles having diameter of $25\sim40 mm$ was significantly higher (p<0.05) than those in normal follicle fluid which has follicles having diameter of $3\sim10 mm$. These results showed that concentration of leptin and insulin in serum were related to BCS value and follicular size and suggest that the changes in concentration of leptin and/or insulin in serum could be a potent biomarker for diagnosis of bovine reproductive disorder.

• Journal of Nutrition and Health
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• v.25 no.3
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• pp.221-232
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• 1992

This study was intended to figure out the interrelationship among body fat distribution serum insulin and lipids levels. One hundred forty four adult female from Chinju area were participated in this study. The survey was conducted between December 17, 1990-February 27, 1991, . The results are as follows : Wiast/hip girth ratio(WHR) and waist/thigh girth ratio(WTR) were increased with age and positively correlated with body mass index(BMI). It appeared that the prevalence of obesity in terms of BMI was higher in upper body type than intermediate or lower body type women. Correlation analyese indicated that serum triglyceride level seemed to be more closely associated with BMI and other body fat distribution indices. Analyses of the anthropometric data serum lipids and insulin were carried out by dividing the sample into three body type groups-upper body type women(WHR$\geq$0, .87) intermediate body type women(0.82$\leq$WHR$\leq$0.86) and low body type women(WHR$\leq$0.81) Age weight BMI RBW percentage of body fat serum insulin triglyceride cholesterol level of upper body type women were significnatly higher than that of intermediate or lower body type women(p<0.05) HDL-cholesterol was significantly lower in upper body type women. These results suggested that body fat distribution would be relevant to chronic metabolic diseases.

• Journal of Nutrition and Health
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• v.28 no.11
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• pp.1056-1064
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• 1995

This study was performed to investigate the difference between obese and non-obese male students. To conduct this study, anthropometric measurements, blood pressure, serum lipids and insulin concentration, and daily nutrients intake of obese and non-obese mal students were measured. The results are as follows : The anthrometric measurements of the two groups showed significant difference in weight (P<0.01), waist(P<0.001), hip(p<0.0001) and thigh (P<0.001) circumference, % of body fat(p<0.0001), kg of body fat(p<0.001) and LBM (lean body mass, p<0.0001). On the other hand, the blood pressure, serum lipids and insulin, and daily nutrients intake of the two groups showed no significant difference. The WHR showed positive correlation with weight, BMI, waist circumference, % of body fat and kg of body fat in obese group. It is also founded that waist circumference, % of body fat, kg of body fat, serum triglyceride, energy and carbohydrate intake in non-obese group showed positive correlation with WHR.

• Journal of Nutrition and Health
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• v.36 no.3
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• pp.239-244
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• 2003

This study investigated the in vivo insulin function of Polygonatum odoratum in normal and diabetic male Sprague-Dawley rats. Diabetes mellitus was induced by an i.p. injection of streptozotocin. Normal and diabetic rats were assigned to the diet groups of the control basal diet and Polygonatum odoratum diet. The animals were fed the diet and water ad libitum for 15 days. Initial and final body weights, total food intake and serum glucose and insulin levels were measured. An insulin suppression test was performed to elucidate the insulin function in the peripheral tissues. The results showed that the final serum glucose levels significantly decreased in the diabetic rats on the Polygonatum odoratum diet compared with the diabetic rats on the control diet. The final serum insulin levels were increased in the diabetic rats on the Polygonatum odoratum diet compared with the diabetic rats on the control diet. The in vivo function of the insulin increased in the diabetic rats on the Polygonatum odoratum compared with the diabetic rats on the control diet. These data indicate that Polygonatum odoratum may be beneficial in improving the in vivo insulin function in streptozotocin-induced diabetic rats.

• Journal of the East Asian Society of Dietary Life
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• v.5 no.1
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• pp.53-66
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• 1995

The purpose of this research is to investigate how obesity and eating behavior are related to the concentration of serum insulin and lipids and blood pressure of middle-aged men The sample for this study utilizes 240 middle-aged men between th4 age of 40 and 60 living in the city of Kumi. Through a process of anthropometry and categorization by relative body weight, the sample is devide into two groups, the obese group (51 men) and the normal group (129 men). The results of the study were as follows : 1111111. The men from the obese group with the exception of height, subs-capular/triceps skinfold girth ratio(STR) had a higher level of body mass index (BMI), waist and hip circumference, waist/hip girth ratio(WHR), subscapular and triceps skinfold thickness than the normal group (P=0.0001). 2. In comparing the serum insulin and lipids between the obese and normal group, the obese group had a higher level of insulin and triglyceride (P=0.016, P=0.050) but a lower concentration of HDL-cholesterol and HDL-cholesterol/total cholesterol (P=0.034, P=0.004). Also, the obese group had a higher level of systolic and diastolic blood pressure (P=0.001, P=0.029). When looking at the relationship between the serum insulin and lipids and anthropometric measurements, the relationship between waist/hip girth ratio(THR), the concentration of fasting serum insulin and lipids were stronger than the obesity index, body mass index(BMI). 3. Men who exercised regularly had a lower total cholesterol and systolic blood pressure 9P=0.049, P=0.041), and a higher level of HDL-cholesterol/total cholesterol (P=0.004). There was no observable relationship between the food habit score, the preference for fiber foods, and the concentration of serum lipids. Also, the concentration of serum lipids had no apparent effect on the preference for salty, sweet, and greasy foods.

• Nutrition Research and Practice
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• v.8 no.5
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• pp.544-549
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• 2014

BACKGROUND/OBJECTIVES: Obesity-associated insulin resistance is a strong risk factor for type 2 diabetes mellitus. The aim of this study was to investigate the effect of myricetin on adiposity, insulin resistance, and inflammatory markers in mice with diet-induced insulin resistance. MATERIALS/METHODS: Five-week-old male C57BL/6J mice were fed a basal diet, a high-fat, high-sucrose (HFHS) diet, or the HFHS diet containing 0.06% myricetin or 0.12% myricetin for 12 weeks after a 1-week adaptation, and body weight and food intake were monitored. After sacrifice, serum lipid profiles, glucose, insulin, adipocyte-derived hormones, and proinflammatory cytokines were measured. The homeostasis model assessment for insulin resistance (HOMA-IR) was determined. RESULTS: Myricetin given at 0.12% of the total diet significantly reduced body weight, weight gain, and epidydimal white adipose tissue weight, and improved hypertriglyceridemia and hypercholesterolemia without a significant influence on food intake in mice fed the HFHS diet. Serum glucose and insulin levels, as well as HOMA-IR values, decreased significantly by 0.12% myricetin supplementation in mice fed the HFHS diet. Myricetin given at 0.12% of the total diet significantly reduced serum levels of leptin, tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6) in mice fed the HFHS diet. CONCLUSIONS: These findings suggest that myricetin may have a protective effect against diet-induced obesity and insulin resistance in mice fed HFHS diet, and that alleviation of insulin resistance could partly occur by improving obesity and reducing serum proinflammatory cytokine levels.

• Journal of Nutrition and Health
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• v.32 no.2
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• pp.189-196
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• 1999

This study was conducted to investigate the relationships among the levels of fasting serum insulin, free fatty acid, lipids and anthropometry in normal-weight female college students. Serum insulin and free fatty acid levels were negatively correlated. However, insulin, triglyceride, and cholesterol levels were not correlated with fat mass or BMI(body mass index). The ability of anthropometric and biochemical indicators to predict fatness could be important for epidemiologic and clinical research. In the subjects, waist cirumference was correlated with BMI, fat mass and percent body fat, and HDLC/CHOL (high density lipoprotein cholesterol/cholesterol)was correlated with body weight, BMI, and fat mass. Our data suggests than waist circumference and HDLC/CHOL are predictors of fatness, whereas WHR apears to be a less important contributor to fatness in female college students.