• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.410-415
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• 2002

Adrenal glucocorticoids, secreted by the stimulus of adrenocorticotropic hormone (ACTH), are catabolic hormones in the pig. The present study was conducted to find whether active immunization against ACTH would suppress cortisol secretion accompanied by an increased growth rate in growing-finishing barrows. ACTH was conjugated to keyhole limpet hemocyanin or human histone using glutaraldehyde or 3-maleimidobenzoic acid N-hydroxysuccinimide, under a 2 (ACTH vs no hapten)${\times}$2 (carrier)${\times}$2 (crosslinker) factorial arrangement of treatments. Cross-bred barrows weighing approximately 25 kg were injected with an ACTHcarrier or carrier only conjugate every 4th wk and slaughtered at approximately 110 kg body weight. Antibodies against ACTH were detected in serum, as determined by $[^{125}I]$ACTH-binding activity, in most animals immunized against the ACTH conjugate, but not in carrier only-injected animals, except for the animals which had received the hapten conjugated to histone via glutaraldehyde. The $[^{125}I]$ACTH-binding activity of serum increased after the second booster injection, but overall ACTH antibody titer was very low. Main effect was not detected not only for the carrier and crosslinker but for the hapten in serum cortisol concentration, ADG, loin muscle area, backfat thickness and longissimus muscle composition including fat and protein. In addition, bound $[^{125}I]$ACTH percentage had no relation to cortisol concentration or to any of the above growth-related variables. Results suggest that ACTH or its conjugates used in the present study were not immunogenically potent enough to affect the glucocorticoid secretion and thus the growth of the immunized pigs.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.2
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• pp.115-118
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• 2000

The production of cultured coho salmon (Oncorhpchus kisutoh) in Korea has being increased year after year. Smolt being reared in freshwater suffer transferring into seawater and are farmed in cages for fattening. This handling processes including transportation, confinement into cages are unavoidable stress to fish in salmon farming and often end up to mass mortality, This study aimed to investigate the impact of handling process on the stress responses of coho salmon. The indicator of stress was measured by cortisol to be a first response, and for the second response test, glucose, triBlyceride, cholesterol, lactate and electrolyte of $K^+, Na^+, Cl^-$ in serum and the activities of alanine aminotrtnferase (ALT), aspartate aminotransferase(AST) and lactate dehydrogenase (LDH) were analyzed. As a result, the concentration of cortisol, glucose as well as LDH activity were significantly increased, whereas others showed no difference comparing with control group. It obviously demonstrated that handling process made fish stressful.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.5
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• pp.682-688
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• 1999

Thirty nine pregnant Javanese thin-tail ewes (20 and 19 carried a single and multiple [2 to 3] fetuses, respectively), and six nonpregnant ewes as controls were used to measure maternal serum hormone and blood metabolite concentrations as predictors of number of fetuses carried during pregnancy. Serum hormones (progesterone, estradiol, triiodothyronine, and cortisol) and blood metabolites (b-hydroxy butyric acid [BHBA], and blood urea nitrogen [BUN]) were determined every four weeks during pregnancy and were used to predict litter size by discriminant analysis. The results of data analysis indicated that serum progesterone and estradiol concentrations at weeks 8, 12, 16 of pregnancy could be used to predict the number of fetuses carried with precision of 86.7 to 95.6%. Serum triiodothyronine, cortisol, BHBA, and BUN concentrations during pregnancy, however, were not good predictors of the number of fetuses carried. Serum progesterone and estradiol concentrations as early as 8 weeks of pregnancy in sheep could predict the number of fetuses carried with 86.7% precision.

• The Korean Journal of Pain
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• v.27 no.1
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• pp.30-34
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• 2014

Background: Myofascial pain dysfunction syndrome (MPDS), otherwise called myofascial pain is one of the most common temporomandibular disorders, which in turn is the most common cause of orofacial pain of non-dental origin. Its etiology is multifactorial and still poorly understood. Psychological factors have been shown to play a role in the etiology. The aim of the study was to evaluate the association between anxiety and salivary cortisol levels in patients with myofascial pain. Methods: Twenty patients suffering from myofascial pain were recruited as the study group. The same number of age and sex matched healthy individuals were taken as the control group. The salivary samples collected between 9-9:15 am from both groups were analyzed for cortisol levels with the competitive enzyme-linked immunosorbent assay method. Anxiety levels of 40 patients were measured using Hamilton's anxiety scale. Results: The mean serum cortisol level of the MPDS group showed a highly significant difference (P < 0.001) from the controls. The mean anxiety scores of the MPDS group showed a highly significant difference (P < 0.001) from the controls. A positive correlation was found between anxiety and the salivary cortisol levels in MPDS patients. Conclusions: These findings suggest that anxiety plays a vital role in the etio-pathogenesis of MPDS; thus, besides pharmacological treatment, psychological support is also needed.

• Journal of Veterinary Clinics
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• v.22 no.2
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• pp.136-140
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• 2005

This experiment is designed to determine the relationship between groomers' activities and stress. The experimenter worked with 2 female dogs and 3 male dogs owned by clients. They were 22 months old on an average and weighed 5.5kg on an average. The concentration of cortisol in blood was measured with radioimmunoassy(RIA). The concentration of cortisol in blood was high in the morning and evening and was low in the afternoon. In a normal condition, the concentration of cortisol in blood was $1.4{\mu}g/dL$, which increased to $4.98{\mu}g/dL$ after 1 hr of grooming, and then returned to normal at the termination of grooming. The survey shows that grooming acted as a stressor, which seems to have an influence on the health of dogs. So it is absolutely necessary that systematic and constant research should be conducted to reduce stress at the time of grooming.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.87-89
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• 1993

Cortisol levels in plasma are known to be as an indication of reproductive and adrenal status of an animal. In this study it has been examined in relation to the oestrus induction by Progesterone oestrogen therapy in 3rd and 4th parity anoestrus animals. Cortisol was found higher in treated animals and levels raised within 6-12 hrs. after hormone therapy followed by elevation in glucose levels and depletion of total serum proteins. It shows the association of induction, occurrence and expression of oestrus with energy demanding metabolic stress in buffaloes.

• Journal of Hospice and Palliative Care
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• v.18 no.2
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• pp.105-111
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• 2015

Purpose: Fatigue, energy loss, feeling of helplessness, poor appetite, pain besides general weakness are major symptoms presented to terminally ill cancer patients. These symptoms are similar to those that appeared with adrenal insufficiency. Also, for terminally ill cancer patients who are hospitalized for palliative care, opioid agents are prescribed to control moderate to severe pain. We studied the relationship of opioid agents and adrenal insufficiency. Methods: From November 2013 through June 2014, we monitored the serum level of cortisol and dehydroepiandrosterone sulfate (DHEAS, serum) in 55 cancer patients who were over 18 years old and were treated at a hospice center. We also checked the treatment period and dosage of opioid agents. Results: The DHEAS level, treatment period and dosage of opioid agents did not have significant correlation. Correlation between the serum cortisol level and the opioid agent treatment period was not significant either, but the serum cortisol level was positively correlated with the dosage of opioid agents (P value 0.0322). Conclusion: This study did not identify a novel link between treatment period, dosage of opioid agents and adrenal insufficiency. But, the DHEAS level was mostly below the normal level in patients who were treated with opioid agents.

• The Korean Journal of Nuclear Medicine
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• v.19 no.2
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• pp.69-79
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• 1985

To evaluate the effect of dopaminergic activity on aldosterone secretion, the plasma renin activity, serum cortisol and aldosterone were measured by radioimmunoassay in 6 normal controls and 12 patients who had hyponatremia and generalized edema or ascites with possible condition with secondary aldosteronism before and after(15, 30, and 60 min) 15 mg of metoclopramide by iv bolus injection and same method with 500 mg of L-dopa by per oral in 6 normal controls and 12 patients with edema ascites. The result were as follows; 1) The basal level of PRA was higher in patients rather than normal controls but PRA was not influenced by MC or L-dopa administration on both normal controls and patients group. 2) The serum cortisol level was significantly elevated at 30 min after MC injection compared with basal level in normal controls but no significant change was noted in patients group. After L-dopa administration the serum cortisol level was not changed in both normal controls and patients group. 3) The serum aldosterone level was significantly elevated in 15, 30 and 60 min after MC injection in normal controls, and there also same tendency of aldosterone secretion was noticed in patients group. On the other hands, there was no changes in aldosterone level in both normal controls and patients group with L-dopa administration. Above result means that MC stimulate aldosterone secretion by dopaminergic antagonist and aldosterone secretion in normal subject is controlled by maximal tonic dopaminergic inhibition. In edematous patients, however, both of the dopaminergic inhibitory and stimulating effect of PRA, ACTH etc on the aldosterone secretion seems to be variable.

• The Journal of Korean Academic Society of Nursing Education
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• v.13 no.2
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• pp.169-176
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• 2007

Purpose: The purpose of this study was to verify the effect of aromatherapy hand massage on stress responses and serum Immunity of RN's before the night duty. Method: The data were collected during the period from Oct. 10 to Nov. 15, 2005 at S-Hospital in Seoul. There were forty-eight subjects, 24 for the experimental and 24 for the control group. A Pre & post test were conducted to measure pulse rate, perceived stress(level and score) and a blood sample for cortisol and IgA. In the experimental group, lavender and geranium were used. Data were analyzed by descriptive statistics, $x^2$-test and t-test with SPSS program. Result: The pulse rate was reduced but there were no significant differences between the two groups. Scores of subjective stress(VAS) decreased significantly after aromatherapy hand massage and the level of serum Cortisol was reduced significantly. The level of serum IgA was elevated significantly. Conclusion: Aromatherapy hand massage could be a useful intervention to reduce stress and elevate the immunity of nurses during the night duty.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.361-368
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• 2012

Effect of vitamin C (VC) on biochemical, hematological and cortisol profile of growing Holstein calves during summer was investigated. Eighteen calves between 14 and 16 weeks of age were divided equally into two groups. One group received a diet supplemented with VC (20 g/d) for 60 days, while other non-supplemented diet fed group served as a control (CON). The temperature humidity index (THI) was recorded and computed during the experiment. From days 0 to 60, the THI exceeded 70. Blood samples were collected from the jugular vein of each calf at days 0, 15, 30, 45 and 60. Serum albumin and total protein decreased (p<0.05) in CON and VC calves with age. Serum glutamic-oxaloacetic transaminase concentrations were not affected by treatments. Serum creatinine, albumin and glutamic-pyruvic transaminase concentrations were higher in calves in the VC group than the CON group. While red blood cells, hemoglobin and hematocrit were lower (p<0.05) in VC calves, mean corpuscular volume, mean corpuscular hemoglobin, red blood cell distribution width and mean platelet volume were higher (p<0.05) in these VC supplemented calves. Leukocyte parameters including white blood cells and full term for lymphocytes were not affected by the treatments. Also, serum cortisol was not affected by treatments. At day 15, 30 and 45, the total VC in plasma was higher (p<0.05) in calves fed with VC. In conclusion, serum cortisols were not affected by plasma VC concentration, while some blood parameters were positively influenced in calves fed with VC.