• 분석과학
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• 제31권5호
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• pp.201-207
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• 2018

혈액은 범죄현장에서 가장 흔하게 볼 수 있는 체액 중 하나이며 용의자를 확인하고 사건 현장을 재구성하는데 중요한 역할을 한다. 혈액의 혈청학적 식별법은 법과학 분야에서 널리 사용되고 있지만, 오래된 혈흔에 대한 혈청학적 식별법의 비교에 관한 연구는 거의 수행되지 않았다. 본 연구의 목적은 오래된 혈흔의 다양한 식별법을 비교하고 그 유효성에 대한 정보를 제공하는 것이다. 본 연구에서는 Tetramethylbenzidine, $Bluestar^{(R)}$, Leucomalachite Green, Kastle-Meyer의 네 가지 혈액 예비시험과 Fecal Occult Blood (FOB)와 Rapid Stain $Identification^{(TM)}-Blood$ ($RSID^{TM}-Blood$) 두 가지 혈액 확증시험을 사용하여 비교연구를 수행하였다. 혈흔 시료는 최소 7년부터 최대 30년동안 실온에서 거즈에 보관된 부검 혈액을 사용하였다. 실험 결과 네 가지 예비 실험에서는 30년까지 경과된 시료를 포함하여 모든 시료에서 양성반응으로 관찰되었다. 반면에 FOB와 $RSID^{TM}-Blood$는 17년 이상 경과된 일부 시료(1988년부터 2001년)에서 음성이 나타나 오래된 혈흔 시료의 경우 확증시험에서 음성반응이 나타날 수 있음을 확인하였다. 이러한 연구 결과는 오래된 증거물에 대한 혈청학적 감정에서 적절한 혈액 식별법 선정에 도움이 될 수 있을 것으로 기대한다. 더불어 법과학적인 감정분야에서 활용할 때 유용한 배경지식이 되리라 사료된다.

• Journal of Microbiology and Biotechnology
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• 제12권1호
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• pp.53-58
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• 2002

To better understand the reservoir host range and distribution of hantaviruses in small mammal populations in Korea, a serological survey was conducted on 1,375 wild rodents and 62 insectivores captured in seven provinces during the six-year period, 1995 to 2000. As determined by the indirect immunofluorescent antibody (IFA) test, 90 ($13.1\%$) of 685 Apodemus agrarius, 47 ($13.6\%$) of 345 Apodemus peninsulae, and 4 ($6.5\%$) of 62 Crocidura laciura were seropositive against the Hantaan virus, while 38 ($13.5\%$) of 282 Eothenomys regulus were seropositive against the Puumala virus. Serological evidence for hantavirus infection was not found in 50 Microtus fortis, six Micromys minutus, six Mus musculus, and one Cricetulus triton. Our serological data indicate that hemorrhagic fever with renal syndrome (HFRS)-related hantaviruses are widely distributed in indigenous rodents in Korea. Particularly noteworthy was the high seropositivity rates among Apodemus peninsulae and Eothenomys regulus captured in certain mountainous regions, suggesting that HFRS may be under-reported among nearby residents or among individuals who might visit such areas for recreational or occupational purposes.

• 대한수의학회지
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• 제34권2호
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• pp.321-326
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• 1994

돼지 유행성 설사 바이러스(KPEDV-9)주를 이용하여 세포내 증식성을 비롯한 혈청학적 역학조사를 수행하였던 바 다음의 결과를 얻었다. 돼지 유행성 설사바이러스 국내 분리주는 Vero 세포에 연속계대시 증식성이 증가되었으며 90대 계대시 $10^{5.5}TCID_{50}/ml$의 역가를 나타내었다. 조직배양 순화주를 이용하여 간염세포내에서 20Kb 이상의 RNA가 존재함을 확인할수 있었으며 전자현미경 검사시 5~10nm의 외피항원 및 80~300nm크기의 coronavirus특징을 나타내었다. 설사증상을 나타내는 돼지의 장가검재료를 이용하여 유행성 설사 바이러스의 감염실태를 조사하였던 바 18%에 상당하는 감염 양성율을 확인하였으며 ELISA법에 의한 항체검사결과 전국적으로 약 45%의 항체 양성율을 나타내었다.

• 한국임상수의학회지
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• 제4권1호
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• pp.409-421
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• 1987

For the epidemiological studies of the respiratory infection from 1,918 heads of adult rabbits from January 1980 to December 1985 at an integrated farm, the causative agents were. Isolated samples 92 from of lungs and nasal discharges, and identified by serological and non-serololgical tests, the loss rates by the infection were also investigatyed, and the antimicrobial sensitivities of the isolates were determined. The results obtained were as fellows : 1. The loss rate by respiratory infection was 11.9% among 1,819 heads which raised and 41.7% among 518 heads which lost by diseases, and the rate increased annually. 2. The loss rate of rabbits in Spring (45.0%), Summer (45.5%) and Winter (53.5%) showed significantly higher (P < 0.01) than that in Autumn(29.1%). 3. The loss rate of Rex (56.3%) showed significantly higher (P<0.05) than New Zealand White (40.7%) and Californian (42.0% ). 4. P. multocida.(37.9%), B. brouchiseptica (16.0%), E. coli (10.6%) and so forth were isolated from 92 samples of lungs and nasal discharges. 5. Sixty fours trains of P. multocida were typed by serological or non-serological tests and their all serotypes were A type (62 strains) except 2 strains of untypable. 6. In antimicrobial sensitivity test for 64 isolates of P. multocida, all the strains tested were highly sensitive to chloramphenicol(95.3%), colistin (95.3%), gentamicin (92.2%), kanamycin (90.6%), neomycin (82.8%) and tetracycline (81.3%), but exhibited low sensitivity to streptomycin (26.6%), and all strains were resistant to lincomycin. 7 Twenty seven isolates of B. brouchiseptica showed higher sensitivity to cephalothin (100%), erythromycin (100% ), gentamicin (100%). trimethoprim+sulfamethoxazole (100%), chloramphenicol(88.9%), kanamycin(88.9%), neomycin (88.9%) and colistin (85.2%), but lower sensitivity to nitrofurantoin (18.5%), penicillin(18.5%). streptomycin(18.5%) and ampicillin (14.8%), and all strains were resistant to lincomycin.

• 한국동물위생학회지
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• 제24권4호
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• pp.359-367
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• 2001

A serological survey was performed to establish basic data for the prevalence of antibodies to some major diseases of domesticated boar serum samples from January to December 2000. Sera collected in breeding farms in Gyeongbuk province were tested for Aujeszky's disease virus(ADV), Porcine reproductive and respiratory syndrome virus(PRRSV), Porcine parvovirus(PPV), Japanese encephalitis virus (JEV), Bordetella bronchiseptica(B bronchiseptica), Mycoplasma ; APP), Toxoplasma, and Brucella. There was no antibody to ADV in domesticated boars serum samples detected by Anti-ADV-gpI assay kit. Sero-positive samples to PRRS by IFA were 0.9%(3/330) The HI titers to PPV ranged variously from less than 10 to over 1,280. Two hundred ninety-four out of 330 tested sera showed HI titer of less than 10. In HI test to JEV, 90.3% of the sera (298/330) were below 10. The majority of the serum samples had low prevalence of the antibody B bronchiseptica. ELISA titers to M hyopneumoniae ranged variously from $\leq$ 10 to $\geq$ 1,280. Antibody titers to A pleuropneumoniae type 2(APP2) and type 5(APP5) were 58.2% and 52.7%, respectively, and the tested samples showing ELISA antibody titers of less than 20. There was no significant geographical difference between APP2 and APP5 in this study. In the antibody test of Toxoplasma, 11.5%(38/330) were positive and samples were all negative in sera test of Brucella.

• Asian-Australasian Journal of Animal Sciences
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• 제10권1호
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• pp.141-146
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• 1997

Postmortem examination was conducted on 350 (three hundred and fifty) chickens. Related samples (Liver, heart, ovary, spleen, bone-marrow, and caecal junction) were collected. The appropriate materials from the samples were cultured into different media. A total 40(forty) isolates of salmonella pullorum and S. gallinarum were identified and preserved. Characterization of the isolates were done by cultural, morphological, biochemical, and serological tests. Salmonella pullorum antigen was prepared from the local isolate, standardized and tested. This antigen was used in the field for the detection of pullorum or fowl typhoid infection or carrier birds. The antigen consisted of suspension of Salmonella pullorum in 0.50 percent sodium chloride plus 1.5 percent sodium sulfate and inactivated with 1% formalin U.S.P. and standardized with McFarland scale iv or by pour plate method containing 800 million organisms per milliliter and stained by the addition of alcoholic crystal violet. Sterility, safety and potency were tested and found as good as other international antigens. The antigen was found to retain its quality for six months when preserved at room temperatures. The test was made by mixing one drop of the antigen with a drop of blood or a drop of serum, on a glass plate or white tile. The locally produced antigen was as good as antigens from Japan, Hungary, Holland and India. A serological study was conducted with the locally prepared antigen in different farms, and the incidence was 0-4% in government farms, 5-10% in commercial imported breeds and 0-3% in cross breed local farms respectively.

• 혜화의학회지
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• 제8권1호
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• pp.827-836
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• 1999

Numerous persons complained dyspepsia even though no specific objective findings are revealed by imaging study, laboratory examinations and other clinical research. To find out of so many symptoms what they are, I would to approach by two ways. One way is oriental medical literatural study and the other is Helicobacter pylori infection that is accepted as one of most important causal factors of many gastric diseases. Background/Aims: Recently, the role of Helicobacter pylori as a causal factor in the etiology of gastric cancer, peptic ulcer, gastritis and low-grade gastric mucosa-associated lymphoid tissue(MALT) lymphoma is well known. Using endoscopy, biopsy urease testing and histology are recommanded as the tests of choice. Serological test is not recommanded at the moment because of its low sensitivity and espicially low specificity. The urea breath test is more sensitive and specific noninvasive test than serologic test, but it is not widely available yet. Methods/Results: We studied 90 cases by diagnostic endoscopy as a screening test for the persons complaining gastrointestinal symptoms. As a result eighteen persons are revealed to be Helicoacter pylori infected histologicaly. Conclusion: More specific literatural studies are requied.

• 한국동물위생학회지
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• 제27권3호
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• pp.273-280
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• 2004

The studies were performed for the PRRS antigen and antibody detection from breeding farms, artificial insemination(AI) center and growing farms in Jeonbuk province. 1. Specific PRRS primers were successfully amplified ORF6 617bp and ORF7 448 bp on agarose gel. 2. RT-PCR method has been establish by commercial kit and the thermal cycler program consisted of 30 cycles: $95^{\circ}C$ for 30 sec, $45^{\circ}C$ for 30 sec, and $72^{\circ}C$ for 45 sec. 3. The results of PRRS antibody test by ELISA method in AI centers were $6.6\%,\;53.3\%$ and breeding farms $65\%,\;65\%\;and\;38.7\%$, respectively. The serological positive of the antibody in gilt higher than sow. 4. The sero-positive of the PRRS antibody showed average $21\%$ in domestic farms, $56.2\%$ in breeding farms, and $29.9\%$ in AI center.

• 한국동물위생학회지
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• 제17권3호
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• pp.174-180
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• 1994

The disease syndrome characterized by the acute vomiting and diarrhea with high mortality had been greatly epidemic in Kyungbuk West Area since March 1990 and it was followed serologically for the classification of the agent. The agent present in feces of dogs associated with this syndrome had characteristic feature in agglutinating pig red blood cells that was specifically inhibited by anti-CPV reference dog serum. This also showed the serological identity with the reference CPV antigen in Hemagglutinating inhibition test. The result obtained were summarized as follows : 1. During 5 years(March. 1990∼September. 1994), 1,470 dogs were investigated on the actual condition of CPV infections. The Infection rate of CPV from dogs was 62.5% and mortality rate was 59.8%. 2. Among 24 fecal samples collected from the dogs with enteric disease, all showed the hemagglutinating activity to porcine erythrocyte ranging from 40 to 5,120 of HA titers. 3. Among 12 sera samples collected from the dogs with enteric disease, all showed the serological identity with the reference CPV antigen from 5 to 5,120 of HI titers. 4. Bacteriologic examination of fecal specimens resulted in the isolation of pathogeric bacteria such as Staphylococcus sp, Streptococcus sp, Escherichia coli and Bacillus. Cultures for salmonella sp and Clostridium remaind negative. 5. The prevalence and identification of internal parasites were determined by fecal examination using the floatation methods. From 20 fecal samples 12(60.0%) were isolated and their species were Toxacara canis, Toxascaris leonina, and coccidium.

• Parasites, Hosts and Diseases
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• 제47권1호
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• pp.61-63
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• 2009

Although Toxoplasma gondii infection generally shows subclinical courses in adults, it may have severe pathologic effects on the fetus. For a survey of toxoplasmosis in Shahr-e-Rey area, south of Tehran, Iran, we conducted a serological study on 1,187 general populations using indirect fluorescent antibody (IFA) test. In this cross sectional study, cases were selected randomly by a local primary health care (PHC) system. Blood samples were collected in microcapillary tubes and sera were examined by IFA after centrifugation. It has been shown that 68.3% of cases in all age groups had IFA titer of 1:10 and higher, which we consider as positive cases, and the rate for 1:400 or over was 30.2%. There was meaningful correlation between anti-Toxoplasma antibody titers and age, sex, and occupation of subjected people (P < 0.05%). The incidence was higher in females and highest in the children age group (10-14 years old). The present study demonstrates high prevalence of Toxoplasma infection among the people in south of Tehran, Iran.