• Parasites, Hosts and Diseases
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• 제37권4호
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• pp.265-270
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• 1999

The gene encoding Plasmodium vivax circumsporozoite protein (PvCSP) exhibits polymorphism in many geographical isolates. The present study was designed to investigate polymorphism in PvCSP gene of P. vivax isolates in Korea. Thirty isolates, obtained from indigenous cases in Yonchon-gun, Kyonggi-do in 1997, were subjected for sequencing and RFLP analysis of the repeat and post-repeat regions of PvCSP gene and two genotypes (SK-A and SK-B) were identified. The genotype of 19 isolates was SK-A and that of 11 isolates was SK-B. Although the number of 12-base repeats present in SK-A was three while two were found in a Chinese strain CH-5, the repeat sequence of SK-A was identical to that of CH-5 except for one base substitution. Compared with known data there was no identical isolates with SK-B, but the sequence of SK-B was similar to that of a North Korean (NK) isolate. These results indicate that two genotypes of PvCSP coexist in the present epidemic area of Korea and the present parasite may originate from East Asia. RFLP would be useful to classify genotypes of P. vivax population instead of gene sequencing.

• 한국산림과학회지
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• 제104권4호
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• pp.549-557
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• 2015

본 연구에서는 잣나무 엽록체 DNA의 전체 염기서열을 기반으로 엽록체 SSR(chloroplast simple sequence repeat) 영역을 특이적으로 증폭하는 primer를 개발하고 그 특성을 분석하였다. 잣나무 엽록체 DNA에서 총 30개의 SSR 영역을 탐색하였으며, 이들 영역을 증폭하기 위한 30개의 primer를 제작하였다. 모든 primer가 잣나무를 대상으로 PCR 증폭이 가능하였다. 근연종에 대한 primer의 종간 전환률은 잣나무와 동일한 아속(Subgenus Strobus)에 속하는 눈잣나무(100%)와 섬잣나무(97%)에서 가장 높게 나타났다. 반면 소나무아속(Subgenus Pinus)에 속하는 소나무와 구주소나무에서의 종간 전환률은 73%로 비교적 낮게 나타났다. 점봉산 잣나무 집단을 대상으로 조사한 결과 13개의 유전자좌에서 다형성이 관찰되었으며, 평균 haploid 다양도(H)는 0.512로 계산되었다. 다형적 유전자좌로부터 조합된 haplotype의 수(N)는 25개로 확인되었고, haplotype 다양도($H_e$)는 0.992로 매우 높게 나타났다. 집단내 독특하게 관찰되는 haplotype은 22개(88%)로 전체 28개체 중에서 22개체(79%)를 식별하였다. 본 연구에서 개발한 cpSSR primer는 높은 종간 전환률을 나타냄에 따라 소나무속의 근연종, 특히 잣나무아속 수종에 활용 가능성이 높고, 잣나무 유전변이 분석을 위한 충분한 다형성을 제공하는 유용한 표지자로 판단된다.

• 한국버섯학회지
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• 제19권1호
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• pp.76-80
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• 2021

느타리 품종구분을 위한 마커의 개발을 위하여 곤지7호의 어버이 일핵 균사중의 하나인 MT07156-97의 전체 유전자 염기서열을 바탕으로 제작한 251개의 SSR 프라이머를 제작하였다. 우선적으로 수한1호, 곤지7호, 흑타리 품종에 다형성 여부를 관찰하여 20개의 SSR을 선발하고, 이를 10개 품종에 적용하였다. 단일의 프라이머로는 일부 품종이 구분되지 않았으므로, 선발된 프라이머 간의 다양한 조합(multiplex 방식)을 적용한 결과 모든 품종을 판별할 수 있는 분자마커 다형성을 보인 프라이머 "166+115" 조합을 선발하였다. 별도로 프라이머 115와 166가 만들어 낸 산술적인 유전자좌(loci) 31개보다 12개 많은 40개의 유전자좌가 증폭되어 다양한 품종에 특이적인 분자마커를 제공할 수 있었다. 개발된 분자마커는 종균의 품질관리, 품종의 판별, 신품종 보호에 활용될 수 있을 것이다.

• 생명과학회지
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• 제32권9호
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• pp.690-697
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• 2022

본 연구는 홍해삼의 유전체를 분석하여 홍해삼의 유전자 마커 개발을 위한 기초 자료로 활용하기 위해 수행되었다. 울릉도_일반과 울릉도_토착으로 microsatellite marker 분석을 실시하였다. 그 결과 dinucleotide repeat 서열이 81.3~81.4%로 가장 많이 차지 되었으며, 반복서열 개수가 증가될수록 감소되는 경향을 보였다. 일반적으로 microsatellite는 5~10 반복수 사이에 집중적으로 존재하였으며, 반복 서열의 크기가 클수록 반복수가 적어지는 양상을 보였다. Di, tri, tetra-nucleotides 반복에서 각각 (AT)₅, (AAT)₅, (AAAT)₅ 등이 가장 높은 것들로 나타났다. (CG), (CCG) 등은 동일 반복 단위의 다른 반복 단위에 비교하여 매우 낮게 관찰되었다. Di-와 tri-nucleotide는 반복수가 각각 35와 32까지 지속적으로 나타난 다음에 비연속적으로 44와 43 반복까지 계수 되었다. Tetra-, penta- 및 hexa-nucleotide는 각각 25, 21 및 14까지 연속적으로 나타났다. 본 분석결과에 따르면 microsatellite는 특이서열반복에 대해 편중되는 경향성을 보이는 것으로 나타났다. 따라서 홍해삼의 microsatellite 분석에서 고유의 반복 서열과 반복수를 유지하는 것으로 추정되므로 향후 연구를 위한 기초 자료로 활용하는 것이 가능할 것으로 판단된다.

• Genomics & Informatics
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• 제7권4호
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• pp.181-186
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• 2009

Myotonic dystrophy type 1 (DM1), which is a dominantly inherited neurodegenerative disorder, results from a CTG trinucleotide repeat expansion in the 3'-untranslated region (3'-UTR) of the myotonic dystrophy protein kinase (DMPK) gene. Retention of mutant DMPK (mDMPK) transcripts in the nuclei of affected cells has been known to be the main cause of pathogenesis of the disease. Thus, reducing the RNA toxicity through elimination of the mutant RNA has been suggested as one therapeutic strategy against DM1. In this study, we suggested RNA replacement with a trans -splicing ribozyme as an alternate genetic therapeutic approach for amelioration of DM1. To this end, we identified the regions of mDMPK 3'-UTR RNA that were accessible to ribozymes by using an RNA mapping strategy based on a trans-splicing ribozyme library. We found that particularly accessible sites were present not only upstream but also downstream of the expanded repeat sequence. Repair or replacement of the mDMPK transcript with the specific ribozyme will be useful for DM1 treatment through reduction of toxic mutant transcripts and simultaneously restore wild-type DMPK or release nucleus-entrapped mDMPK transcripts to the cytoplasm.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.586-589
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• 2003

Previously we reported the cloning and sequence analysis of a CFTase gene from Bacillus polymyxa. CFTase was divided into five distinct regions. In order to understand a role of the N-terminal repeat region on the function of CFTase from Bacillus polymyxa MGL21, deletion mutantCFTase ${\Delta}N$ was prepered. Recombinant protein was overproduced in E. coli as inclusion body, solubilized in bufer containing 8M urea, and refolded in the phosphate buffer. The molecular weight of the purified wild type CFTase and CFTase ${\Delta}N$ were 148kDa , 108kDa, respectively.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.79-86
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• 2005

Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
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• pp.51-54
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• 2003

Analysis of the Neurospora crassa genome data reveals at least 14 proteins that contain tetratricopeptide repeat (TPR) motifs. One of them shows over $60\%$ homology with SSN6 of Saccharomyces cerevisiae, a global repressor that mediates repression of genes involved in various cellular processes. Sequence analysis of its cDNA shows that it encodes a putative 102kDa protein. Mutant strains generated by RIP (repeat induced point mutation) process show four distinctive patterns of vegetative growth at various rates. They are male-fertile, yet all female-sterile and produced little or no perithecium. These results indicate that this gene is pleiotropic and involved in several cellular processes of vegetative growth, conidiation and sexual cycle. It is designated rcm-1(regulation of conidiation and morphology).

• BMB Reports
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• 제29권1호
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• pp.63-67
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• 1996

In order to elucidate the molecular mechanism of plasmid incompatibility of broad host-range plasmid R1162, the plasmid-encoded replication protein RepIB was purified and tested for binding to the 20 bp direct repeat (DR) DNA sequence which is reiterated 3 and 1/2 times within the replicative origin of the plasmid. The RepIB protein specifically binds to the DR DNA. Point mutations in the DR which affect expression of plasmid incompatibility also coordinately affect binding. These results indicate that the incompatibility of broad host-range plasmid R1162 is exerted by the DR DNA by titrating the essential replication protein RepIB.

• ETRI Journal
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• 제26권5호
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• pp.405-412
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• 2004

MC-CDMA, a multicarrier (MC) modulation scheme based on code division multiple access (CDMA), is the most likely candidate for the next generation of mobile radio communications. The rate compatible punctured turbo (RCPT) coded hybrid automatic repeat request (HARQ) has been found to give improved throughput performance in a direct sequence (DS) CDMA system. However, the extent to which the RCPT HARQ improves the throughput performance of an MC-CDMA system has not been fully understood. In this paper, we apply the RCPT HARQ to MC-CDMA and evaluate by computer simulations its performance in a frequency selective Rayleigh fading channel. We found that the performance of RCPT HARQ MC-CDMA is almost insensitive to channel characteristics. The performance can be drastically improved with receive diversity combined with space-time transmit diversity. In addition, the comparison of RCPT HARQ MC-CDMA, orthogonal frequency division multiplexing, and DS-CDMA shows that under similar conditions the throughput of MC-CDMA is the best in a frequency selective fading channel.