• Toxicological Research
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• v.17
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• pp.109-115
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• 2001

Abrus agglutinin was purified from the kernels of Abrus precatorius by Sepharose 4B affinity column chromatography followed by Sephadex G-100 gel filtration column chromatography. About 1.25 g of abrus agglutinin was obtained from 1 kg of the kernels. The LD$_{50}$ of abrus agglutinin is 5 mg/kg of body weight, which is less toxic than that of abrin, 20$\mu\textrm{g}$/kg body weight. The amino acid sequence of abrus agglutinin was determined by protein sequencing techniques and deduced from the nucleotide sequence of a cDNA clone encoding full length of abrus agglutinin. There are 258 residues, 2 residues and 267 residues in the A-chain, the linker peptide and the B-chain of abrus agglutinin, respectively. Abrus agglutinin had high homology to abrin-a (77.8%). The 13 amino acid residues involved in catalytic function, which are highly conserved among abrin and ricin, were also conserved within abrus agglutinin. The protein synthesis inhibitory activity of abrus agglutinin ($IC_{50}$/ = 3.5 nM) was weaker than that of abrin-a (0.05 nM). By molecular modeling followed by site-directed mutagenesis showed that Pro199 of abrus agglutinin A-chain located in amphipathic helix H and corresponding to Asn200 of abrin A-chain, can induce bending of helix H. This bending would presumably affect the binding of abrus agglutinin A-chain to its target sequence GpApGpAp, in the tetraloop structure of 285 r-RNA subunit and this could be one of major factors contributing to the relatively weak protein synthesis inhibitory activity and toxicity of abrus agglutinin.n.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.2
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• pp.642-648
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• 2016

As 3D solid modeling prevails, a range of applications have become possible and intensive research on the integration of CAD/CAM has been conducted. As a consequence, methods to recognize the machining features from CAD models have been developed. On the other hand, generating a machining sequence using the machining features is still a problem due to a combinatorial problem with a large number of machining features. This paper proposes a new method that utilizes the precedence constraints through which the number of the combinations is reduced drastically. This method can automatically generate machining sequences requiring the lowest amount of machining time. An airplane part was used to test the usefulness of the proposed method.

• Steel and Composite Structures
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• v.19 no.5
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• pp.1095-1113
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• 2015

In order to determine the mechanical behavior of the curved laminates in practical applications, three right-angled composite brackets with different lay-ups were investigated both experimentally and numerically. In the experimental, quasi-static tests on both unidirectional and multidirectional curved composite brackets were conducted to study the progressive failure and failure modes of the curved laminates. In the numerical modeling, three-dimensional finite element analysis was used to simulate the mechanical behavior of the laminates. Here, a strength-based failure criterion, namely the Ye criterion, was used to predict the delamination failure in the composite curved laminates. The mechanical responses of the laminate subjected to off-axis tensile loading were analyzed, which include the progressive failure, the failure locations, the load-displacement relationships, the load-strain relationships, and the stress distribution around the curved region of the angled bracket. Subsequently, the effects of stacking sequence and thickness on the load carrying capacity and the stiffness of the laminates were discussed in detail. Through the experimental observation and analysis, it was found that the failure mode of all the specimens is delamination, which is initiated abruptly and develops unstably on the symmetric plane, close to the inner surface, and about $29^{\circ}$ along the circumferential direction. It was also found that the stacking sequence and the thickness have significant influences on both the load carrying capacity and the stiffness of the laminates. However, the thickness effect is less than that on the curved aluminum plate.

• The KIPS Transactions:PartB
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• v.17B no.3
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• pp.183-188
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• 2010

A factorization-based 3D reconstruction system is realized to recover 3D scene from an image sequence. The image sequence is captured from uncalibrated perspective camera from several views. Many matched feature points over all images are obtained by feature tracking method. Then, these data are supplied to the 3D reconstruction module to obtain the projective reconstruction. Projective reconstruction is converted to Euclidean reconstruction by enforcing several metric constraints. After many triangular meshes are obtained, realistic reconstruction of 3D models are finished by texture mapping. The developed system is implemented in C++, and Qt library is used to implement the system user interface. OpenGL graphics library is used to realize the texture mapping routine and the model visualization program. Experimental results using synthetic and real image data are included to demonstrate the effectiveness of the developed system.

• The Journal of Engineering Geology
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• v.13 no.3
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• pp.369-378
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• 2003

The statistical modeling was introduced to satisfy various experimental conditions on the sorption of heavy metals (Pb, Cu, Cd, and Zn) by clay minerals, i.e. kaolinite, illite and chlorite. The Box-Behnken model designed statistically was applied to determine a relative impact among three variables such as pH, HCO3(or K) concentration and initial concentration of heavy metals. The SAS program was used to obtain the statistical solution by surface response analysis. The results of a statistical sorption modelling indicated that pH is a strong impact of the variables influencing the sorption of heavy metals. A relative effect between an initial concentration of heavy metals and bicarbonate(or K) concentration is dependent on solution condition. The sorption edge of heavy metals as function of pH shows sigmoidal curve, and a great increase in the range of pH 6～8. The sorption sequence among heavy metals is Cu>Pb>>Zn>Cd. The solution chemistry exerts greater influence on the sorption of heavy metals rather than the crystal chemistry of clay minerals. The potassium exerts some effect into a sorption competition with heavy metals. The research suggests that the statistical modeling is an effective method to demonstrate sorption results in three dimension and to reduce the effort of batch sorption experiment.

• Journal of Internet Computing and Services
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• v.23 no.4
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• pp.21-33
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• 2022

Recently, the recommender system has been widely used in various fields such as movies, music, online shopping, and social media, and in the meantime, the recommender model has been developed from correlation analysis through the Apriori model, which can be said to be the first-generation model in the recommender system field. In 2005, many models have been proposed, including deep learning-based models, which are receiving a lot of attention within the recommender model. The recommender model can be classified into a collaborative filtering method, a content-based method, and a hybrid method that uses these two methods integrally. However, these basic methods are gradually losing their status as methodologies in the field as they fail to adapt to internal and external changing factors such as the rapidly changing user-item interaction and the development of big data. On the other hand, the importance of deep learning methodologies in recommender systems is increasing because of its advantages such as nonlinear transformation, representation learning, sequence modeling, and flexibility. In this paper, among deep learning methodologies, RNN, CNN, and GAN-based models suitable for sequential modeling that can accurately and flexibly analyze user-item interactions are classified, compared, and analyzed.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.2
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• pp.344-352
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• 2003

An effective algorithm for implementation of which detects moving object from image sequences. predicts the direction of it. and drives the camera in real time is proposed. In static camera, for robust motion detection from a dynamic background scene, the proposed algorithm performs statistical modeling of moving objects and background, and trains the statistical modeling of moving objects and background, and trains the statistical feature of background with the initial parts of sequence which have no moving objects. Active camera moving objects are segmented by following procedure, an improved order adaptive lattice structured linear predictor is used. The proposed algorithm shows robust object tracking results in the environment of static or active camera. It can be used for the unmanned surveillance system, traffic monitoring system, and autonomous vehicle.

• Journal of the Korea Institute of Military Science and Technology
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• v.18 no.5
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• pp.594-601
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• 2015

The purpose of this research is to propose a maintenance support system for deciding assembly sequence of the product and appropriate tools that are used to assembly and disassembly of parts in the product when geometric properties of the product. The digital maintenance system (DMS) is developed to generate the maintenance guideline and the initial experiment is conducted especially for an underwater weapon system with cylindrical structure. DMS considers four factors to find the efficient assembly and disassembly procedure automatically: (1) assembly tree, (2) properties of each part, (3) distance from the center of the product, and (4) volume. Based on the factors, DMS simulate the movement of each tool virtually and the properties of tools are investigated to find an appropriate tool for using assembly and disassembly of each part in the product. The proposed approach integrates modeling, simulation, data configuration, and virtual reality to allow a development of preliminary maintenance guidance.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.182-186
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• 2012

human tyrosinase (hTyr) catalyzes first and the rate limiting step in the synthesis of polymerized pigment, melanin which determines skin, hair and eye colors. Mutation of hTyr often brings about decrease of melanin production and further albinism. Meanwhile, a number of cosmetic companies providing skincare products for woman in Asia-Pacific region have tried to develop inhibitors to bright skin color for several decades. In this study, we built a 3D structure by comparative modeling technique based on the crystal structure of tyrosinase from bacillus megaterium as a template to serve structural information of hTyr. According to our model and sequence analysis of type 3 copper protein family proteins, two copper atoms of active site located deep inside are coordinated with six strictly conserved histidine residues coming from four-helix-bundle. Cavity which accommodates substrates was like funnel shape of which entrance was wide and expose to solvent. In addition, protein-substrate and protein-inhibitor complex were modeled with the guide of van der waals surface generated by in house software. Our model suggested that only phenol group or its analogs can fill the binding site near nuclear copper center because inside of binding site has narrow shape relatively. In conclusion, the results of this study may provide helpful information for designing and screening new anti-melanogensis agents.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.3
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• pp.167-177
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• 2009

This paper is about our experience of developing a context-aware exhibit guide system called MyGuide by applying Simtext development methodology. In particular, we introduce some issues to be considered in development process, the way that we solved them, and other things related to context. In requirements elicitation phase, we reduced the complexity of requirements by separating business logic requirements and context-awareness related requirements. After that, we modeled context, and designed software architecture, classes, and database in sequence during design phase. We considered system requirements and context modeling issues for context modeling because it influences class and database design.