• Journal of Sericultural and Entomological Science
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• 제51권2호
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• pp.137-141
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• 2013

In this study, nucleotide sequence structures of intergenic transcribed spacer (ITS) 1, complete 5.8S ribosomal RNA gene and ITS 2 region were analyzed to identify three Peruvian entomopathogenic fungal isolates. The isolates had highly conserved sequence region in 5.8S rRNA gene and unique sequences in ITS 1 and 2 region among them. 5.8S rRNA gene regions were highly conserved and showed high homoloies among tested isolates. In contrast, ITS region showed species-specific sequence region, resulting in inter-genus differencies. Scanning electron microscopic images of these isolates supported the result of ITS-based identification. From these result, Peruvian entomopathogenic fungal isolate J270, J278, were identified as Beauveria bassiana and J271 was identified as Lecanicillium attenuatum.

• Korean Journal of Plant Resources
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• 제17권2호
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• pp.82-93
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• 2004

Phylogenetic relationships among species in Araliaceae were analyzed using PCR-RAPD and sequence of ITS region of nuclear ribosomal DNA based on samples collected in Korea. RAPD analysis showed various polymorphic bands which were able to differentiate species and genus, and specific bands showing variations among individuals within species. Cluster analysis using gel images revealed high molecular variability within species of Aralia eleta. No significant variation was found among cultivated species of Panax ginseng, but they showed high genetic differences with wild type of the species. In ITS analysis, specific sequences for each genus and species were observed and these were allowed to differentiate species and genus. Phylogenetic analysis using ITS sequences showed that Acanthopanax and Kalopanax had a close relationship, and Aralia and Panax are monophyletic, but genus Hedera is different species from other species in family Araliaceae in this study. The results showing close relationship between genera Aralia and Panax were also observed in RAPD analysis. Contrary to the results of RAPD analysis of Panax ginseng, sequence analysis of ITS showed no significant difference between wild mountain ginseng and cultivated species of P. ginseng. Also, both RAPD and ITS analysis of P. ginseng showed no significant genetic variability among cultivation sites. Results indicate that P. ginseng cultivating in Korea is monophyletic. The molecular analysis used in this study agreed on classification using morphological feature. These results suggest that molecular techniques used in this study could be useful for phylogenetic analysis of Araliaceae.

• Journal of Biomedical Engineering Research
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• 제27권5호
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• pp.203-209
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• 2006

The aim of this study is to investigate the feasibility of ex vivo MR diffusion tensor imaging technique in order to observe the diffusion-contrast characteristics of human gastric tissues. On normal and pathologic gastric tissues, which have been fixed in a polycarbonate plastic tube filled with 10% formalin solution, laboratory made 3D diffusion tensor Turbo FLASH pulse sequence was used to obtain high resolution MR images with voxel size of $0.5{\times}0.5{\times}0.5mm^3\;using\;64{\times}32{\times}32mm^3$ field of view in conjunction with an acquisition matrix of $128{\times}64{\times}64$. Diffusion weighted- gradient pulses were employed with b values of 0 and $600s/mm^2$ in 6 orientations. The sequence was implemented on a clinical 3.0-T MRI scanner(Siemens, Erlangen, Germany) with a home-made quadrature-typed birdcage Tx/Rx rf coil for small specimen. Diffusion tensor values in each pixel were calculated using linear algebra and singular value decomposition(SVD) algorithm. Apparent diffusion coefficient(ADC) and fractional anisotropy(FA) map were also obtained from diffusion tensor data to compare pixel intensities between normal and abnormal gastric tissues. The processing software was developed by authors using Visual C++(Microsoft, WA, U.S.A.) and mathematical/statistical library of GNUwin32(Free Software Foundation). This study shows that 3D diffusion tensor Turbo FLASH sequence is useful to resolve fine micro-structures of gastric tissue and both ADC and FA values in normal gastric tissue are higher than those in abnormal tissue. Authors expect that this study also represents another possibility of gastric carcinoma detection by visualizing diffusion characteristics of proton spins in the gastric tissues.

• Progress in Medical Physics
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• 제24권1호
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• pp.25-34
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• 2013

To investigate effects of phase mask on susceptibility-weighted images (SWI) using voxel-based analyses in normal elderly subjects. A three-dimensional (3D) gradient echo sequence ran to obtain SWIs in 20 healthy elderly subjects. SWIs with two (SWI2) and four (SWI4) phase multiplications were achieved with positive (PSWI) and negative (NSWI) phase masks to investigate phase mask effects. The voxel-based comparisons were performed using paired t-tests between PSWI and NSWI and between SWI2 and SWI4. Differences of signal intensities between magnitude images and SWI4 were larger than those between magnitude images and SWI2s. Differences of signal intensities between magnitude images and PSWIs were larger than those between magnitude images and NSWIs. Moreover, the signal intensities from NSWI2s and NSWI4s were greater than those from PSWI2s and PSWI4s, respectively. More differences of signal intensities between NSWI4 and PSWI4s were found than those between NSWI2s and PSWI2s in the whole brain images. The voxel-based analyses of SWI could be beneficial to investigate susceptibility differences on the entire brain areas. The phase masking method could be chosen to enhance brain tissue contrast rather than to enhance venous blood vessels. Therefore, it is recommended to apply voxel-based analyses of SWI to investigate clinical applications.

• Journal of Institute of Control, Robotics and Systems
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• 제13권11호
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• pp.1060-1066
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• 2007

When a high-energy ultrasound propagates through a solid body that contains a crack or a delamination, the two faces of the defect do not ordinarily vibrate in unison, and dissipative phenomena such as friction, rubbing and clapping between the faces will convert some of the vibrational energy to heat. By combining this heating effect with infrared imaging, one can detect a subsurface defect in material efficiently. In this paper a detection of the welding defect of thin SUS 304 plates using the UIR (ultrasonic infrared imaging) technology is described. A low frequency (20kHz) ultrasonic transducer was used to infuse the welded thin SUS 304 plates with a short pulse of sound for 280ms. The ultrasonic source has a maximum power of 2kW. The surface temperature of the area under inspection is imaged by a thermal infrared camera that is coupled to a fast frame grabber in a computer. The hot spots, which are a small area around the defect tip and heated up highly, are observed. From the sequence of the thermosonic images, the location of defective or inhomogeneous regions in the welded thin SUS 304 plates can be detected easily.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권6호
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• pp.2767-2780
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• 2016

Video-based human-activity recognition has become increasingly popular due to the prominent corresponding applications in a variety of fields such as computer vision, image processing, smart-home healthcare, and human-computer interactions. The essential goals of a video-based activity-recognition system include the provision of behavior-based information to enable functionality that proactively assists a person with his/her tasks. The target of this work is the development of a novel approach for human-activity recognition, whereby human-body-joint features that are extracted from depth videos are used. From silhouette images taken at every depth, the direction and magnitude features are first obtained from each connected body-joint pair so that they can be augmented later with motion direction, as well as with the magnitude features of each joint in the next frame. A generalized discriminant analysis (GDA) is applied to make the spatiotemporal features more robust, followed by the feeding of the time-sequence features into a Hidden Markov Model (HMM) for the training of each activity. Lastly, all of the trained-activity HMMs are used for depth-video activity recognition.

• Proceedings of the Korea Contents Association Conference
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• 한국콘텐츠학회 2006년도 추계 종합학술대회 논문집
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• pp.195-199
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• 2006

In the field of video design, motion graphics tends to be realized to deliver a certain message properly incorporating basic motion graphics components such as space, form of expression, and time, and has continued to be used as a powerful communication tool. Many cases proved that title sequences were produced as a result of combination of a variety of quick images and effects with appropriately chosen sounds to meet the demands of audience. This study indicated that motion graphics began to be widely used as a more powerful video communication tool for title sequences and also applied for M-NET, DMB, CABLE TV, IPTV, and others.

• Journal of the Korean Institute of Intelligent Systems
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• 제13권4호
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• pp.461-468
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• 2003

In a sequence of images obtained by surveillance cameras, facial regions appear very small and their colors change abruptly by lighting condition. This paper proposes a new face detection scheme, robust on complex background, small size, and lighting conditions. The proposed method is consisted of three processes. In the first step, the candidates for the face regions are selected using face color distribution and motion information. In the second stage, the non-face regions are removed using face color ratio, boundary ratio, and average of column-wise intensity variation in the candidates. The face regions containing eyes and mouth are segmented and classified, and then they are scored using their topological relations in the last step. To speed up and improve a performance the above process, a block based image segmentation technique is used. The experiments have shown that the proposed algorithm detects faced regions with more than 91% of accuracy and less than 4.3% of false alarm rate.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 한국퍼지및지능시스템학회 2003년도 ISIS 2003
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• pp.603-607
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• 2003

The judgment of forged passports plays an important role in the immigration control system, for which the automatic and accurate processing is required because of the rapid increase of travelers. So, as the preprocessing phase for the judgment of forged passports, this paper proposed the novel method for the recognition of passport based on the fuzzy binarization and the fuzzy RBF neural network newly proposed. first, for the extraction of individual codes being recognized, the paper extracts code sequence blocks including individual codes by applying the Sobel masking, the horizontal smearing and the contour tracking algorithm in turn to the passport image, binarizes the extracted blocks by using the fuzzy binarization based on the membership function of trapezoid type, and, as the last step, recovers and extracts individual codes from the binarized areas by applying the CDM masking and the vertical smearing. Next, the paper proposed the enhanced fuzzy RBF neural network that adapts the enhanced fuzzy ART network to the middle layer and applied to the recognition of individual codes. The results of the experiment for performance evaluation on the real passport images showed that the proposed method in the paper has the improved performance in the recognition of passport.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제13권1호
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• pp.50-58
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• 2013

Estimating a driver's head pose is an important task in driver-assistance systems because it can provide information about where a driver is looking, thereby giving useful cues about the status of the driver (i.e., paying proper attention, fatigued, etc.). This study proposes a system for estimating the head pose using monocular images, which includes a novel use of backprojection. The system can use a single image to estimate a driver's head pose at a particular time stamp, or an image sequence to support the analysis of a driver's status. Using our proposed system, we compared two previous pose estimation approaches. We introduced an approach for providing ground-truth reference data using a mannequin model. Our experimental results demonstrate that the proposed system provides relatively accurate estimations of the yaw, tilt, and roll angle. The results also show that one of the pose estimation approaches (perspective-n-point, PnP) provided a consistently better estimate compared to the other (pose from orthography and scaling with iterations, POSIT) using our proposed system.