• The Korea Journal of Herbology
• /
• v.37 no.5
• /
• pp.9-16
• /
• 2022

Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry of Food and Drug Safety(MFDS). Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation, respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification using species-specific primers. DNA barcoding analysis used sequence information of the matK region. Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species 'P. ternata' of genuine 'Pinelliae Tuber', and 23 were identified as adulterant species 'P. pedatisecta'. Unlike this, the genetic identification was identified as the original species 'P. ternata' in all 50 samples in the SCAR marker and matK regional sequence analysis. Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of the morphological mutant.

• Biomedical Science Letters
• /
• v.24 no.4
• /
• pp.430-434
• /
• 2018

Candida glabrata is the second most prevalent causative agent for candidiasis following C. albicans. The opportunistic yeast, C. glabrata, is able to cause the critical bloodstream infections in hospitalized patients. Conventional identification methods for yeasts are often time consuming and labor intensive. Therefore, recent studies on sequence-based identification have been conducted. Recently, sequencing the D1/D2 domain of the large subunit ribosomal RNA gene and the internal transcribed spacers (ITS) 1 and ITS2 regions of the ribosomal DNA has proven useful for DNA-based identification of most species of fungi. In the present study, therefore, fungal ITS and D1/D2 domain regions were targeted and analyzed by DNA sequencing for the accurate identification of C. glabrata clinical isolates. A total of 102 C. glabrata clinical isolates from various clinical samples including bloodstream, catheterized urine, bile and other body fluids were used in the study. The results of the DNA sequence analysis showed that the mean standard deviation of species identity percent score between ITS and D1/D2 domain regions was $97.8%{\pm}2.9$ and $99.7%{\pm}0.46$, respectively. These results revealed that the D1/D2 domain region might be a better target for identifying C. glabrata clinical isolates based on DNA sequences than the ITS1 and ITS2 regions. However, in order to evaluate the usefulness of D1/D2 domain region for species identification of all Candida species, other Candida species such as C. albicans, C. tropicalis, C. dubliniensis, and C. krusei should be verified in further studies additionally.

• Korean Journal of Microbiology
• /
• v.44 no.4
• /
• pp.333-338
• /
• 2008

Computational analysis of partial rpoB gene sequence (777 bp) was done in this study to identify B. anthracis and its closely related species B. cereus and B. thuringiensis. Sequence data including 17 B. anthracis strains, 9 B. cereus strains, and 7 B. thuringiensis strains were obtained by searching databases. Those sequences were aligned and used for other computational analysis. B. anthracis strains were identificated by in silico restriction enzyme digestion. B. cereus and B. thuringiensis were not segregated by this method. Those sequencing and BLAST search were required to distinguish the two. In actual identification tests, B. anthracis strains could be identified by PCR-RFLP, and B. cereus and B. thuringiensis strains were distinguished by BLAST search with reliable e-value. In this study fast and accurate method for identifying three Bacillus species, and flow chart of identification were developed.

• Korean Journal of Food Science and Technology
• /
• v.32 no.6
• /
• pp.1331-1335
• /
• 2000

The WL6 strain isolated from Kimchi could not be made scientific name because it was identified as three species, i.e., Leuconostoc mesenternides ssp cremoris, Leu. mesenteroides ssp. dextranicum or Lactobacillus bifermentans when it was tested by API kit or Biolog system methods. The unidentifiable WL6 strain was finally reclassified as Lactobacillus bifermentans by genetic identification using two PCR-based specific sequence primer sets which were originated from homologous pepN and 16S rRNA genes.

• Mycobiology
• /
• v.39 no.4
• /
• pp.299-300
• /
• 2011

Two species of cultivated Phellinus sp. were identified as P. baumii by internal transcribed spacer (ITS) sequence analysis. The fruit bodies of the examined strains were similar to those of naturally occurring strains, having a bracket-like form, yellow-to-orange color, and poroid hymenial surfaces. The DNA sequences of ITS region of both strains showed a homology of 99% with ITS1 to ITS2 sequences of P. (Inonotus) baumii strain PB0806.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.1
• /
• pp.127-136
• /
• 2015

Cupredoxin-like proteins are mainly copper-binding proteins that conserve a typical rigid Greek-key arrangement consisting of an eight-stranded β-sandwich, even though they share as little as 10-15% sequence similarity. The electron transport function of the Cupredoxins is critical for respiration and photosynthesis, and the proteins have therapeutic potential. Despite their crucial biological functions, the identification of the distant Cupredoxin homologs has been a difficult task due to their low sequence identity. In this study, the overlapped conserved residue (OCR) fingerprint for the Cupredoxin superfamily, which consists of conserved residues in three aspects (i.e., the sequence, structure, and intramolecular interaction), was used to detect the novel Cupredoxin homologs in the NCBI non-redundant protein sequence database. The OCR fingerprint could identify 54 potential Cupredoxin sequences, which were validated by scanning them against the conserved Cupredoxin motif near the Cu-binding site. This study also attempted to model the 3D structures and to predict the functions of the identified potential Cupredoxins. This study suggests that the OCR-based approach can be used efficiently to detect novel homologous proteins with low sequence identity, such as Cupredoxins.

• 제어로봇시스템학회:학술대회논문집
• /
• 1998.10a
• /
• pp.280-284
• /
• 1998

Many classes of nonlinear systems can be represented by Volterra kernel expansion. Therefore, identification of Volterra kernels of nonlinear system is an important task for obtaining the nonlinear characteristics of the nonlinear system. Although one of the authors has recently proposed a new method for obtaining the Volterra kernels of a nonlinear system by use of M-sequence and correlation technique, our mettled of nonlinear system identification is limited to Wiener-type nonlinear system and we can not apply this method to the identification of Hammerstein-type nonlinear system. This paper describes a new mettled for obtaining Volterra kernels of Hammerstein nonlinear system by adding a linear element in front of tile Hammerstein system. First we calculate the linear element of Hammerstein system by use of conventional correlation method. Secondly, we put a linear element in front of Hammerstein system. Then the total system becomes Wiener-type nonlinear system. Therefore we can use our method on Volterra kernel identification by use of M-sequence. Thus we get the coefficients of the approximation polynomial of nonlinear element of Hammerstein system. From the results of simulation, a good agreement with theoretical considerations is obtained, showing a wide applicability of our method.

• International Journal of Computer Science & Network Security
• /
• v.22 no.11
• /
• pp.241-247
• /
• 2022

Ad hoc network is self-organized network powered by battery. The reliability of virtual user identification and channel security are reduced when SNR is low due to limited user energy. In order to solve this problem, a joint virtual user identification and channel security en/decoding method is proposed in this paper. Transmitter-receiver-based virtual user identification code is generated by executing XOR operation between orthogonal address code of transmitter and pseudo random address code of receiver and encrypted by channel security code to acquire orthogonal random security sequence so as to improve channel security. In order to spread spectrum as well as improve transmission efficiency, data packet is divided into 6-bit symbols, each symbol is mapped with an orthogonal random security sequence. Subspace-based method is adopted by receiver to process received signal firstly, and then a judgment model is established to identify virtual users according to the previous processing results. Simulation results indicate that the proposed method obtains 1.6dB E_b/N₀ gains compared with reference methods when miss alarm rate reaches 10^-3.

• Korean Journal of Food Science and Technology
• /
• v.30 no.3
• /
• pp.655-659
• /
• 1998

Bacterial strain showing the strong fibrinolytic activity (2.04 plasmin unit) was screened from Jeot-Gal, Korean salt-fermented fish collected from various region. For the identification, when the strain was characterized morphologically, culturally, and biochemically, it was identified to Bacillus pumilus. And, when the fatty acids composition of the strain was analyzed, it was identified to Bacillus atropheus. Finally, the 16S rRNA partial sequence (V3 region) showed that the fibrinolytic stain screened from Jeot-Gal was identified as Bacillus subtilis. So, we named it Bacillus subtilis KJ-48.

• 제어로봇시스템학회:학술대회논문집
• /
• 1996.10a
• /
• pp.161-164
• /
• 1996

The authors have recently proposed a new method for identifying Volterra kernels of nonlinear control systems by use of M-sequence and correlation technique. A specially chosen M-sequence is added to the nonlinear system to be identified, and the crosscorrelation function between the input and output is calculated. Then every crosssection of Volterra kernels up to 3rd order appears at a specified delay time point in the crosscorrelation. This method is applied to a saturation-type nonlinear feedback control system of mechanical-electrical servo system having torque saturation nonlinearity. Simulation experiments show that we can obtain Volterra kernels of saturation-type nonlinear system, and a good agreement is observed between the observed output and the calculated one from the measured Volterra kernels.