• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1998.03a
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• pp.134-137
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• 1998

Caliber design in bar and rod rolling depends on the designer's experience, which in general is acquired through costly trial-and-error process. As a prerequisite for developing a scientific approach to caliber design, we present a finite element model to simulate 3-D deformation of bars and rods occurring in multi-pass sequence. The results are compared with measurements obtained from POSCO for to assess the solution accuracy. The comparison shows that the simulation results agree well experiments.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.14 no.24
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• pp.111-121
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• 1991

This paper considers a non preemtive single processor scheduling problem in which each set have the two common due dates. The objective of the problem is to minimize the weighted mean absolute deviation of job completion times about such two common due dates under the assumption that each job has a different weight. Such a job sequence is an W-shaped sequence. We propose three heuristic solution methods based on several dominance conditions. Numerical examples are presented. The performance comparison is made among three heuristic scheduling procedures.

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.153-153
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• 1995

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.195.1-195
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• 1996

No Abstract, See Full Text

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1998.06a
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• pp.331-333
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• 1998

• Journal of the Korea Society of Computer and Information
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• v.24 no.1
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• pp.93-100
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• 2019

One of critical issues in dealing with 360-degree realistic contents is the performance degradation in searching and recognition process since they support up to 4K UHD quality and have all image angles including the front, back, left, right, top, and bottom parts of a screen. To solve this problem, in this paper, we propose an efficient search and comparison method for 360-degree realistic contents. The proposed method first corrects the distortion at the less distorted regions such as front, left and right parts of the image excluding severely distorted regions such as upper and lower parts, and then it extracts feature points at the corrected region and selects the representative images through sequence classification. When the query image is inputted, the search results are provided through feature points comparison. The experimental results of the proposed method shows that it can solve the problem of performance deterioration when 360-degree realistic contents are recognized comparing with traditional 2D contents.

• BMB Reports
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• v.32 no.4
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• pp.399-404
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• 1999

2,3-Dihydroxybiphenyl 1,2-dioxygenase (2,3-DHBD), which catalyzes the ring meta-cleavage of 2,3-dihydroxybiphenyl, is encoded by the phnQ gene of biphenyl- and phenanthrene-degrading Pseudomonas sp. strain DJ77. We determined the nucleotide sequence of a DNA fragment of 1497 base pairs which included the phnQ gene. The fragment lncluded an open reading frame of 903 base pairs to accommodate the enzyme. The predicted amino acid sequence of the enzyme subunit consisted of 300 residues. In front of the gene, a sequence resembling an E. coli promoter was identified, which led to constitutive expression of the cloned gene in E. coli. The deduced amino acid sequence of the PhnQ enzyme exhibited 85.6% identity with that of the corresponding enzyme in Sphingomonas yanoikuyae Q1 (formerly S. paucimobilis Q1) and 22.1% identity with that of catechol 1,2,3-dioxygenase from the same DJ77 strain. PhnQ showed broader substrate preference than previously-cloned PhnE, catechol 2,3-dioxygenase. Ten amino acid residues, considered to be important for the role of extradiol dioxygenases, were conserved.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1022-1030
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• 2004

The gene encoding Aquifex pyrophilus (Apy) DNA polymerase was cloned and sequenced. The Apy DNA polymerase gene consists of 1,725 bp coding for a protein with 574 amino acid residues. The deduced amino acid sequence of Apy DNA. polymerase showed a high sequence homology to Escherichia coli DNA polymerase I-like DNA polymerases. It was deduced by amino acid sequence alignment that Apy DNA polymerase, like the Klenow fragment, has only the two domains, the $3'{\rightarrow}5'$ exonuclease domain and the $5'{\rightarrow}3'$ polymerase domain, containing the characteristic motifs. The Apy DNA polymerase gene was expressed under the control of T7lac promoter on the expression vector pET-22b(+) in E. coli. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $UNO^{TM}$ Q column chromatographies. The optimum pH of the purified enzyme was 7.5, and the optimal concentrations of KCl and $Mg^{2+}$ were 20 mM and 3 mM, respectively. Apy DNA polymerase contained a double strand-dependent $3'{\rightarrow}5'$ proofreading exonuclease activity, but lacked any detectable $5'{\rightarrow}3'$ exonuclease activity, which is consistent with its amino acid sequence. The somewhat lower thermostability of Apy DNA polymerase than the growth temperature of A. pyrophilus was analyzed by the comparison of amino acid composition and pressure effect.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.12
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• pp.1816-1821
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• 2003

Reliable PCR based identification of lactobacilli has been described utilizing the sequence of 16S-23S rRNA intergenic spacer region. Those sequence comparisons showed a high degree of difference in homology among the strains of L. rhamnosus, L. casei, L. acidophilus and L. helveticus whose 16S-23S rRNA intergenic small SR's sizes were 222 bp, 222 bp, 206 bp and 216 bp respectively. The sequence of 16S-23S rRNA intergenic spacer region of L. rhamnosus ATCC 53103 revealed the close relatedness to those of L. casei strains by the homology ranges from 95.4% to 97.2%. 16S-23S rRNA intergenic spacer region nucleotide sequence of L. acidophilus showed some distant relatedness with L. rhamnosus ATCC 53103 with the homology ranges from 40.3% to 41.8% and that with L. helveticus was shown to be 30% of homology, which exists at the most distant phylogenetic relatedness. The identification of species and strain of lactobacilli was possible on the basis of these results. The common sequences among the 17 strains were CTAAGGAA located in the initiating position of the DNA and some discrepancies were found between the same strains based on these results.

• Parasites, Hosts and Diseases
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• v.35 no.2
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• pp.105-110
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• 1997

The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chaill termination method. The cloned gene corresponds to 869 bps encoding an open reading frame of 283 amino acids. Comparison of the sequence between Korean and .Tapanese isolates showed 99.4% homology rate in the nucleotide sequence and 98.9% homology rate in the amino acid sequence.