• Title/Summary/Keyword: separation and sorting

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Microfluidic cell sizing using hydrophoretic size-based separation (유체영동 기반의 입자분리현상을 이용한 세포 크기 측정방법)

  • Choi, Sung-Young;Park, Je-Kyun
    • Journal of Sensor Science and Technology
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    • v.17 no.4
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    • pp.245-249
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    • 2008
  • This paper presents a microfluidic cell sizing method using hydrophoretic size-based separation. By exploiting slanted obstacles in a microchannel, we can generate a lateral pressure gradient so that microparticles can be deflected and arranged along lateral flows induced by the gradient. Using such movement of particles, we discriminated 8 to 15 μm-sized beads. We measured the size of U937 cells by comparing the hydrophoretic response of the cells to those of the size-standard beads whose diameters are known. Due to its simple design and fabrication, the sizing method can be easily integrated with other microfluidic components such as cell culture chambers conducting on-chip sizing and sorting.

Development of Automatic Sorting System for Black Plastics Using Laser Induced Breakdown Spectroscopy (LIBS) (LIBS를 이용한 흑색 플라스틱의 자동선별 시스템 개발)

  • Park, Eun Kyu;Jung, Bam Bit;Choi, Woo Zin;Oh, Sung Kwun
    • Resources Recycling
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    • v.26 no.6
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    • pp.73-83
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    • 2017
  • Used small household appliances have a wide variety of product types and component materials, and contain high percentage of black plastics. However, they are not being recycled efficiently as conventional sensors such as near-infrared ray (NIR), etc. are not able to detect black plastic by types. In the present study, an automatic sorting system was developed based on laser-induced breakdown spectroscopy (LIBS) to promote the recycling of waste plastics. The system we developed mainly consists of sample feeder, automatic position recognition system, LIBS device, separator and control unit. By applying laser pulse on the target sample, characteristic spectral data can be obtained and analyzed by using CCD detectors. The obtained data was then treated by using a classifier, which was developed based on artificial intelligent algorithm. The separation tests on waste plastics also were carried out by using a lab-scale automatic sorting system and the test results will be discussed. The classification rate of the radial basis neural network (RBFNNs) classifier developed in this study was about > 97%. The recognition rate of the black plastic by types with the automatic sorting system was more than 94.0% and the sorting efficiency was more than 80.0%. Automatic sorting system based on LIBS technology is in its infant stage and it has a high potential for utilization in and outside Korea due to its excellent economic efficiency.

The Electrode Shape for the Efficient Separation of Cell in Dielectrophoresis-Activated Cell Sorter (유전영동을 이용한 입자분리기의 효율적인 분리를 위한 전극 형태)

  • An, Jae-Min;Chae, Seung-Yeub;Park, Seok-Ho;Kim, Byung-Kyu
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.33 no.1
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    • pp.49-55
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    • 2009
  • This paper presents the optimal shape of microelectrode that generates dielectrophoretic(DEP) force to separate particles in homogeneous medium. The principle of the particles sorting is based on the use of the relative strengths of negative DEP (nDEP) and drag forces, as in a general DEP-activated cell sorter (DACS). To numerically calculate the DEP force and drag force, the simulation is implemented in MATLAB 7.0. The properties of particles, which are used in simulation, are similarly selected as those of cells to apply cell separation. The most optimized shape of electrode is selected by numerical simulation according to a variety of electrode shape such as rectangle, trapezoidal, and right-triangle. Through, in addition, parameter study, we found that applied frequency is more significant factor on the separation than various parameters, such as applied voltage and permittivity of medium, that decide on the strength of DEP force.

Postal Envelope Image Recognition System for Postal Automation (서장 우편물 자동처리를 위한 우편영상 인식 시스템)

  • Kim, Ho-Yon;Lim, Kil-Taek;Kim, Doo-Sik;Nam, Yun-Seok
    • The KIPS Transactions:PartB
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    • v.10B no.4
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    • pp.429-442
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    • 2003
  • In this paper, we describe an address image recognition system for automatic processing of standard- size letter mail. The inputs to the system are gray-level mail piece images and the outputs are delivery point codes with which a delivery sequence of carrier can be generated. The system includes five main modules; destination address block location, text line separation, character segmentation, character recognition and finally address interpretation. The destination address block is extracted on the basis of experimental knowledge and the line separation and character segmentation is done through the analysis of connected components and vortical runs. For recognizing characters, we developed MLP-based recognizers and dynamical programming technique for interpretation. Since each module has been implemented in an independent way, the system has a benefit that the optimization of each module is relatively easy. We have done the experiment with live mail piece images directly sampled from mail sorting machine in Yuseong post office. The experimental results prove the feasibility of our system.

Separation of wasted plastics by thermal adhesion (열 점착을 이용한 혼합 폐 플라스틱의 선택적 분리)

  • Lim Seok ki;Cho Hee chan
    • Resources Recycling
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    • v.12 no.4
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    • pp.44-50
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    • 2003
  • Separation of mixed plastics is not a simple task due to similarities of specific gravity and limitations of sizes, etc. In this study, a new technique was developed to separate plastics selectively by a thermal adhesion method using the different softening or melting temperatures of various types of plastics. Tests were conducted to investigate softening or melting temperatures and adhesion probabilities of plastics with change in temperature. Tests also were conducted to analyze the operating variables, including contact times, size and color pigment. It was founded that LDPE, HDPE and PVC, PET, etc among mixed plastics could be separated selectively according to the change of temperature.

A Study on Physical Properties of Recyclables obtained from MSW (재활용(再活用) 대상(對象) 폐자원( 廢資源)의 물리적(物理的) 성상(性狀) 분석(分析)에 관한 연구(硏究))

  • Choi, Woo Zin;Yoon, Kun Duk
    • Resources Recycling
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    • v.21 no.5
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    • pp.72-78
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    • 2012
  • In general, recyclable items are separately discharged from households and then, hand-sorted into different types of valuables at materials recovery facilities (MRF). However, most of residues after hand-sorting are finally end up with landfill and/or incineration due to lack of separation technologies and economical reasons. In the present study, physical properties on recyclable items obtained from an MRF are investigated to improve the recovery of valuable items. The results of physical compositions based on the sizing tests are also presented.

An Analysis of Voltage Characteristics for LC Resonant Frequency Band of Capacitor Compensation According to Moving of Electrical Separation Equipment of AF Track Circuit (AF궤도회로의 전기적 구분 장치 설치이전에 따른 커패시터 보상으로 LC공진 주파수 대역의 전압특성 분석)

  • Won, Seo-Yeon;Choi, Jae-Sik;Park, Hun-Jue;Kim, Hie-Sik
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.8
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    • pp.1466-1477
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    • 2016
  • This paper analyzes the electrical characteristic such as the impedance(Z), inductance(L), and cable resistance($R_p$) according to the change of cable length in order to move the electrical sorting device for distinguishing between AF non-insulated track circuits from the center of railway to outside railway. The simulation is performed to check the voltage difference between the voltage of sender and the voltage of receiver and determine the possibility of the voltage restoration availability in the frequency filter band through the capacitor compensation. It was applied to the results of the simulation to the sorting devices installed in the actual field. It is proved the availability by checking the measured voltage characteristic according to the capacitor compensating change of $10{\mu}F$ and $16{\mu}F$ before, and after the length of cable is increased with 6 meters. Through this, the prevention of breakdown and damage to facilities and the prevention the safety-related accidents of line workers from the train are expected according to moving the sorting devices of AR non-insulated track circuits to outside railway.

Dried pepper sorting using independent component analysis on RGB images (RGB영상의 독립성분분석을 이용한 건고추영상 분류)

  • Kwon, Ki-Hyeon;Lim, Jung-Dae
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.4
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    • pp.59-65
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    • 2012
  • Hot pepper can be easily faded or discolored in drying process, so we need to use the sorting technique to improve the quality for dried hot pepper. Independent Component Analysis (ICA) is one of the most widely used methods for blind source separation. In this paper we use this technique to get a concentration image of the most important component which plays a role in the dried pepper. This concentration image is different from the binary image and it reflects the characteristics of major components, so that we know the distribution and quality of the component and how to sort the dried pepper. Also, the size of the concentration image can tell the relation with capsaicinoids which make hot taste. We propose a sorting method of the dried hot pepper that is faded or discolored and lacked a major component likes capsaicin in drying process using ICA concentration image.

Magnetic-activated cell sorting improves high-quality spermatozoa in bovine semen

  • de Assumpcao, Teresinha Ines;Severo, Neimar Correa;Zandonaide, Joao Pedro Brandao;Macedo, Gustavo Guerino
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.2
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    • pp.91-98
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    • 2021
  • The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 106 cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 106 cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.

Behavior-induced Disposable Cup Automatic Separation X-Bin Study

  • LEE, Su-Min;KIM, Seo-Hyun;KIM, Ye-Na;BYUN, Hyo-Jeong;KWON, Lee-Seung;JUNG, Min-Jae;KWON, Woo-Taeg
    • Journal of Wellbeing Management and Applied Psychology
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    • v.4 no.1
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    • pp.19-25
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    • 2021
  • Purpose: This study seeks to develop behavior-induced automatic separation X-Bin for disposable cup speculators. Some students throw away the contents. Research design, data and methodology: Therefore, environmental beautification workers spend a lot of time sorting disposable cups at school garbage collection sites. Disposable cups that have been thrown away like this are bad in terms of appearance and smell. In addition, cleaning inside garbage affects recycling rates and is closely related to environmental problems. Results: Clean cleaning reduces the amount of garbage incinerated without being recycled and reduces the cost of recycling. According to Korean Social Trends 2020, COVID-19 began to spread, and recycling of disposable items such as disposable cups increased by about 15 percent from the same period last year. Conclusions: These are classified for recycling, and the amount of garbage thrown on the streets is estimated to be larger. In order to prevent COVID-19s, the use of disposable goods is inevitable, but facilities are needed to dispose of them properly.