• Title/Summary/Keyword: separation analysis

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A rapid separation of Cs, Sr and Ba using gas pressurized extraction chromatography with inductively coupled plasma-mass spectrometry

  • Sojin Jeong;Jihye Kim;Hanul Cho;Hwakyeung Jeong;Byungman Kang;Sang Ho Lim
    • Analytical Science and Technology
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    • v.37 no.2
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    • pp.123-129
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    • 2024
  • We present a rapid method for the determination of Cs, Sr, and Ba, heat generators found in highly active liquid wastes, by gas-pressurized extraction chromatography (GPEC) using a column containing a cation-exchange resin. GPEC is a microscale column chromatographic technique that uses a constant flow rate of solvent (0.07 mL/min) with pressurized nitrogen gas supplied through a valve. In particular, because this method uses a small sample volume (a few hundred microliters), it produces less chemical waste and allows for faster separation compared to traditional column chromatography. In this study, we evaluated the separation of Cs, Sr, and Ba using GPEC. The eluate from the column (GPEC or conventional column chromatography) was quantitatively analyzed using inductively coupled plasma-mass spectrometry to measure the column recovery and precision. The column reproducibility of the proposed GPEC system (RSDs of recoveries) ranged from 2.7 to 4.1 %, and the column recoveries for the three elements ranged from 72 to 98% when aqueous HCl was used as the eluent. The GPEC results are slightly different in efficiency and separation resolution compared to those of conventional column chromatography because of the differences in the eluent flow rate as well as the internal diameter and length of the column. However, the two methods had similar recoveries for Cs and Sr, and the precision of GPEC was improved by two-fold. Remarkably, the solvent volume required for GPEC analysis was five times lower than that of the conventional method, and the total analysis time was 11 times shorter.

Analysis of Broken Rice Separation Efficiency of a Laboratory Indented Cylinder Separator

  • Kim, Myoung Ho;Park, Seung Je
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.95-102
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    • 2013
  • Purpose: Using a laboratory indented cylinder separator, broken rice separation experiments were conducted and the characteristics of the separation process were studied to provide information for developing a prototype indented cylinder broken rice separator. Methods: Rice (Ilmi variety) milled in a local RPC was used for the experiment. Rice kernels were classified into four groups according to their length l; whole kernels (I > 3.75 mm), semi-whole kernels (2.5 < I < 3.75 mm), broken kernels (1.75 < I < 2.5 mm), and foreign matters (I < 1.75 mm). A laboratory grain cleaner, Labofix '90 (Schmidt AG, Germany) was used for the experiments. Experiments were designed as a $4{\times}4$ factorial arrangement in randomized blocks with three replications. Cylinder rotational speeds (17, 34, 51, 68 rpm) and trough angles (15, 37.5, 60, $82.5^{\circ}$) were the two factors and feed rates (25, 50 kg/h), indent shapes (Us, $S_1$ type), and indent sizes (2.5, 3.75 mm) were treated as the blocks. Two 125 g samples and one 125 g sample were taken at the cylinder outlet and from the trough, respectively. The whole, semi-whole, and broken kernel weight ratio of the samples and feed was determined by a rice sizing device. From these weight ratios, purities, degrees of extraction and coefficient of separation efficiency were calculated. Results: Trough angle, cylinder speed, and their interaction on the coefficient of separation efficiency were statistically significant. Cylinder speed of 17, 34, and 51 rpm made the most effective separation when the trough angle was $15^{\circ}$ or $37.5^{\circ}$, $60^{\circ}$, and $82.5^{\circ}$, respectively. Maximum values of coefficient of separation efficiency were in the range of 60 to 70% except when the indent size was 2.5 mm and were recorded for the combinations of low cylinder speed (17 rpm) with medium trough angle ($37.5^{\circ}$ or $60^{\circ}$). Indent shape did not appear to make any noticeable difference in separation efficiency. Conclusions: Due to the interaction effect, the trough angle needs to be increased appropriately when an increase in cylinder speed is made if a rapid drop of effectiveness of separation should be avoided. In commercial applications, $S_1$ type indents are preferred because of their better manufacturability and easier maintenance. For successful separation of broken kernels, the indent size should be set slightly bigger than the actual sizes of broken kernels: an indent size of 3.0 mm for separating broken kernels shorter than 2.5 mm.

Effects of Column Length and Particle Diameter on Phospholipid Analysis by Nanoflow Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

  • Lee, Ju-Yong;Lim, Sang-Soo;Moon, Myeong-Hee
    • Mass Spectrometry Letters
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    • v.2 no.3
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    • pp.65-68
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    • 2011
  • The effects of column length and particle size on the efficiency of separation and characterization of phospholipids (PLs) are investigated using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS-MS). Since PLs are associated with cell proliferation, apoptosis, and signal transduction, it is of increasing interests in lipidomics to establish reliable analytical methods for the qualitative and quantitative profiling of PLs related to biomarker development in adult diseases. Due to the complexity of PLs, the preliminary separation of PLs is necessary prior to MS analysis. In this study, length of capillary column and the particle size of reversed phase ($C_{18}$) packing materials are varied to find a reliable condition for the high speed and high resolution separation using 8 PL standard mixtures. From experiments, it was found that a capillary column of nLC-ESI-MS-MS analysis for PL mixtures can be minimized to a 5 cm long pulled tip column packed with 3 ${\mu}m$ $C_{18}$ particles without losing resolution.

Comparison of Independent Component Analysis and Blind Source Separation Algorithms for Noisy Data (잡음환경에서 독립성분 분석과 암묵신호분리 알고리즘의 성능비교)

  • O, Sang-Hun;Cichocki, Andrzej;Choe, Seung-Jin;Lee, Su-Yeong
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.39 no.2
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    • pp.10-20
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    • 2002
  • Various blind source separation (BSS) and independent component analysis (ICA) algorithms have been developed. However, comparison study for BSS/ICA algorithms has not been extensively carried out yet. The main objective of this paper is to compare various promising BSS/ICA algorithms in terms of several factors such as robustness to sensor noise, computational complexity, the conditioning of the mixing matrix, the number of sensors, and the number of training patterns. We propose several benchmarks which are useful for the evaluation of the algorithm. This comparison study will be useful for real-world applications, especially EEG/MEG analysis and separation of miked speech signals.

An Improved Multiplicative Updating Algorithm for Nonnegative Independent Component Analysis

  • Li, Hui;Shen, Yue-Hong;Wang, Jian-Gong
    • ETRI Journal
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    • v.35 no.2
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    • pp.193-199
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    • 2013
  • This paper addresses nonnegative independent component analysis (NICA), with the aim to realize the blind separation of nonnegative well-grounded independent source signals, which arises in many practical applications but is hardly ever explored. Recently, Bertrand and Moonen presented a multiplicative NICA (M-NICA) algorithm using multiplicative update and subspace projection. Based on the principle of the mutual correlation minimization, we propose another novel cost function to evaluate the diagonalization level of the correlation matrix, and apply the multiplicative exponentiated gradient (EG) descent update to it to maintain nonnegativity. An efficient approach referred to as the EG-NICA algorithm is derived and its validity is confirmed by numerous simulations conducted on different types of source signals. Results show that the separation performance of the proposed EG-NICA algorithm is superior to that of the previous M-NICA algorithm, with a better unmixing accuracy. In addition, its convergence speed is adjustable by an appropriate user-defined learning rate.

Fatigue life prediction for radial truck tires using a global-local finite element method

  • Jeong, Kyoung Moon;Beom, Hyeon Gyu;Kim, Kee-Woon;Cho, Jin-Rae
    • Interaction and multiscale mechanics
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    • v.4 no.1
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    • pp.35-47
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    • 2011
  • A global-local finite element modeling technique is employed in this paper to predict the fatigue life of radial truck tires. This paper assumes that a flaw exists inside the tire, in the local model. The local model uses an FEM fracture analysis in conjunction with a global-local technique in ABAQUS. A 3D finite element local model calculates the energy release rate at the belt edge. Using the analysis of the local model, a study of the energy release rate is performed in the crack region and used to determine the crack growth rate analysis. The result considers how different driving conditions contribute to the detrimental effects of belt separation in truck tire failure. The calculation of the total mileage on four sizes of radial truck tires has performed on the belt edge separation. The effect of the change of belt width design on the fatigue lifetime of tire belt separation is discussed.

Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis (모세관 전기 영동을 이용한 퀴놀린계 항생제의 광학 이성질체 분석)

  • Gang, Dae Cheon;Jo, Seung Il;Jeong, Du Su;Choe, Gyu Seong;Kim, Yong Seong
    • Journal of the Korean Chemical Society
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    • v.46 no.5
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    • pp.412-429
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    • 2002
  • Chiral separation of gemifloxacin, an quinolone antibacterial agent, using (+)-(18-crown-6)-tetracar-boxylic acid $(18C6H_4)$ as a chiral selector was performed by capillary electrophoresis (CE). Direct analysis of quinolone antibacterial agent in body fluid is beneficial in terms of fast analysis time, multicomponent analysis. However, high con-centration of sodium ion in body fluid can prevent gemifloxacin from interacting with $18C6H_4$ since sodium ion has high affinity with $18C6H_4$ due to the strong charge interaction. Ethylenediaminetetraacetic acid (EDTA), as a chelating ligand, was added in the running buffer in order to reduce the interaction between sodium ion and the chiral selector. Increased separation efficiency and reduced migration time were observed while sodium ion exists in the sample solution at the concentration up to 150 mM.

Transition Flow Analysis According to the Change of Reynolds Number for Supersonic Launch Vehicle Fairing Expansion Area (초음속 발사체 선두 팽창부의 레이놀즈수 변화에 따른 천이 유동 해석)

  • Shin, Ho-Cheol;Park, Soo-Hyung;Byun, Yung-Hwan
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.45 no.5
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    • pp.367-375
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    • 2017
  • RANS computational analysis was performed on the head of the launch vehicle including the hammerhead nose pairing in the supersonic regime. The two-dimensional axisymmetric analysis was performed by using laminar, fully turbulent and transition models and compared with the experimental data. It was observed that different flow phenomena occurred depending on the Reynolds number. Under the high Reynolds number condition, the boundary layer becomes turbulent, which is not separated from the surface of the launch vehicle. With the low Reynolds number condition, laminar separation bubble was produced due to the separation and reattachment of the boundary layer on the expansion-compression edge of the hammerhead type nose fairing. The three-dimensional computations with the angle of attack showed a fully detached vortical structure due to the laminar separation bubble. It is proved that the turbulent transition should be considered to predict the separation bubble with the Reynolds number.

Effect of Clearance on Clamping Characteristics of Segmented Nuts in Pyrotechnic Separation Nut (파이로테크닉 분리 너트 내부 분할 너트 체결 특성에 대한 틈새의 영향)

  • Woo, Jeongmin;Kang, Dahoon;Choi, Jaeyoung;Kim, Jeong Ho;Cho, Jin Yeon;Jang, Seung-gyo;Yang, Hee Won
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.46 no.12
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    • pp.994-1003
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    • 2018
  • The clamping characteristics of segmented nuts in pyrotechnic separation nut are investigated using finite element analysis. When the segmented nuts are used to clamp objects, a constraint cylinder is necessary to prevent the segmented nuts from spreading out to the radial direction. During the manufacturing process, a clearance is usually introduced between the outer diameter of the segmented nuts and the inner diameter of the constraint cylinder. Therefore to find out the effect of the clearance, proper finite element modeling method is suggested to describe the clamping procedure appropriately. In addition, finite element analysis with the clearance of 0.00, 0.03, 0.06, and 0.10 mm are performed. From the analysis results, the clamping characteristics of segmented nuts are investigated according to the clearance, and several factors which should be considered to design a reliable pyrotechnic separation nut are figured out.

The Study on Phase Separation Development by Curing Reaction Rate for Unsaturated Polyester/Polyvinylacetate Semi-IPN (Unsaturated Polyester/Polyvinylacetate Semi-IPN의 경화반응속도에 따른 상분리현상 연구)

  • Chang, Won-Young;Kim, Moo-Sool;Kim, Jin-Hwan;Nam, Jae-Do
    • Polymer(Korea)
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    • v.25 no.1
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    • pp.78-89
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    • 2001
  • Morphological changes of unsaturated polyester/polyvinylacetate semi-IPN were studied while the phase separation and the cure reaction occurred in a competing fashion. The light scattering and thermal analysis techniques were used to investigate the phase separation rates and mechanical properties resultantly induced by molecular diffusion of thermoplastic polymer during the curing process of thermosetting polymer. The reaction activation energy was calculated by using Flynn-Wall method and the semi-IPN structure exhibited various phase-separation morphological characteristics. When PVAc composition was 10 wt%, the phase separation was not observed during the curing reaction, but the phase separation occurred in a similar fashion to nucleation and growth(NG) mechanism at room temperature. On the other hand, when PVAc composition was over 11.65 wt%, the phase separation was generated in the middle of the curing process. Consequently, the phase separation seemed to influence the curing reaction rate, which was also supported by the changing activation energy with conversion and PVAc composition. Finally, the total scattered intensity was measured at various temperature, and subsequently the diffusion rates of phase separation R(${\beta}m$) were evaluated.

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